Name: Corynebacterium glutamicum 2247
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 3094

Type strain:

Species: Corynebacterium glutamicum

Strain Designation: 2247

Culture col. no.: DSM 20411, ATCC 14067, DSM 1413, DSM 778, NCIB 9565, JCM 1308, BCRC 11383, CCM 3600, IAM 12433, IFO 12153, KCCM 11318, KCTC 1738, NBRC 12153, NCIMB 9565

Strain history: K. Suzuki CNF 014 <-- AJ 1510 <-- ATCC 14067 <-- Ajinomoto Co., Inc., Japan; 2247.

NCBI tax ID(s): 1079988 (strain), 1718 (species)

SI-ID 35961

ATCC 14067, DSM 20411, DSM 1413, DSM 778, NCIB 9565, JCM 1308, CCRC 11383, IAM 12433, KCTC 1738, NCIMB 9565, CCM 3600, IFO 12153, NBRC 12153, BCRC 11383, NCAIM B.01726

Other strains from Corynebacterium glutamicum

Section

Corynebacterium glutamicum 2247 is a mesophilic bacterium that produces amino acids.

amino acid production
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium glutamicum**
	Full Scientific Name (LPSN)	Corynebacterium glutamicum (Kinoshita et al. 1958) Abe et al. 1967 (Approved Lists 1980)

	Synonym	"Micrococcus* glutamicus"*
	Synonym	Brevibacterium divaricatum
	Synonym	Corynebacterium lilium

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %92.291

Multimedia content

[Ref.: #8797]

Caption:

Medium 535a 28°C

[Ref.: #8797]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8797]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8797]

Culture medium growth

[Ref.: #8797]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8797]

Culture medium composition

expand / minimize

[Ref.: #8797]

Culture medium

TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)

[Ref.: #8797]

Culture medium growth

[Ref.: #8797]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8797]

Culture medium composition

expand / minimize

[Ref.: #8797]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #8797]

Culture medium growth

[Ref.: #8797]

Culture medium link

Medium recipe provided by DSMZ

	Temperatures	Kind of temperature		Temperature
[Ref.: #8797]			growth	28 °C
[Ref.: #67770]			growth	28 °C

[Ref.: #8797]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	no Confidence in %99
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.899

[Ref.: #8797]	Name of produced compound	L glutamic acid
[Ref.: #8797]	Name of produced compound	glutamate synthase

[Ref.: #8797]	Murein short key	A31
[Ref.: #8797]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-9(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	fermentation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #67770]		L-glutamate ChEBI	yes
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #8797]	24959	+	-	+	-	+	-	-	-	+	-	+	+/-	-	+	+	-	+/-	+/-	+	+/-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #8797]	14225	-	-	+/-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8797]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Corynebacterium glutamicum ATCC 14067 16S ribosomal RNA gene, partial sequence	AY017108	633	ENA	1079988 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Corynebacterium glutamicum ATCC 14067	GCA_000233355	contig	GenBank	1079988 tax ID
[Ref.: #66792]	Corynebacterium glutamicum ATCC 14067	GCA_002243555	complete	GenBank	1079988 tax ID	*
[Ref.: #66792]	Corynebacterium glutamicum ATCC 14067	1079988.5	complete	PATRIC	1079988 tax ID	*
[Ref.: #66792]	Corynebacterium glutamicum ATCC 14067	1079988.3	wgs	PATRIC	1079988 tax ID	*
[Ref.: #66792]	Corynebacterium glutamicum ATCC 14067	2848153583	complete	JGI IMG/M	1079988 tax ID	*
[Ref.: #66792]	Corynebacterium glutamicum ATCC 14067	2531839299	draft	JGI IMG/M	1079988 tax ID	*

[Ref.: #8797]	GC-content	54.0 mol%
[Ref.: #67770]	GC-content	54.1 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	56.8 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium glutamicum ATCC 14067 PATRIC: 1079988.5
[Ref.: #69481]	spore-forming	no	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium glutamicum ATCC 14067 ATCC 14067 NCBI: GCA_002243555.1
[Ref.: #69480]	flagellated	no	97.356	no
[Ref.: #69480]	gram-positive	yes	88.157	no
[Ref.: #69480]	anaerobic	no	99.186	no
[Ref.: #69480]	aerobic	yes	74.775	no
[Ref.: #69480]	halophile	yes	64.834	no
[Ref.: #69480]	spore-forming	no	90.652	no
[Ref.: #69480]	glucose-util	yes	87.327	yes
[Ref.: #69480]	thermophile	no	97.643	yes
[Ref.: #69480]	motile	no	93.078	no
[Ref.: #69480]	glucose-ferment	yes	72.726	yes

Availability in culture collections External links

[Ref.: #8797]

Culture collection no.

DSM 20411, ATCC 14067, DSM 1413, DSM 778, NCIB 9565, JCM 1308, BCRC 11383, CCM 3600, IAM 12433, IFO 12153, KCCM 11318, KCTC 1738, NBRC 12153, NCIMB 9565

[Ref.: #72612]

SI-ID 35961

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Multiplex gene editing and large DNA fragment deletion by the CRISPR/Cpf1-RecE/T system in Corynebacterium glutamicum.	Zhao N, Li L, Luo G, Xie S, Lin Y, Han S, Huang Y, Zheng S	J Ind Microbiol Biotechnol	10.1007/s10295-020-02304-5	2020	*
Metabolism	The 138(th) residue of acetohydroxyacid synthase in Corynebacterium glutamicum is important for the substrate binding specificity.	Liu Y, Wang X, Zhan J, Hu J	Enzyme Microb Technol	10.1016/j.enzmictec.2019.06.001	2019	*
Metabolism	Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette.	Huang Y, Li L, Xie S, Zhao N, Han S, Lin Y, Zheng S	Sci Rep	10.1038/s41598-017-08352-9	2017	*
Metabolism	Mutational analysis to identify the residues essential for the inhibition of N-acetyl glutamate kinase of Corynebacterium glutamicum.	Huang Y, Zhang H, Tian H, Li C, Han S, Lin Y, Zheng S	Appl Microbiol Biotechnol	10.1007/s00253-015-6469-5	2015	*
Enzymology	Construction of a novel expression system for use in Corynebacterium glutamicum.	Hu J, Li Y, Zhang H, Tan Y, Wang X	Plasmid	10.1016/j.plasmid.2014.07.005	2014	*
Genetics	Genome sequence of Corynebacterium glutamicum ATCC 14067, which provides insight into amino acid biosynthesis in coryneform bacteria.	Lv Y, Liao J, Wu Z, Han S, Lin Y, Zheng S	J Bacteriol	10.1128/JB.06514-11	2012	*
Metabolism	Microbial production of L -glutamate and L -glutamine by recombinant Corynebacterium glutamicum harboring Vitreoscilla hemoglobin gene vgb.	Liu Q, Zhang J, Wei XX, Ouyang SP, Wu Q, Chen GQ	Appl Microbiol Biotechnol	10.1007/s00253-007-1254-8	2007	*
Proteome	A comparative proteomic approach to understand the adaptations of an H+ -ATPase-defective mutant of Corynebacterium glutamicum ATCC14067 to energy deficiencies.	Li L, Wada M, Yokota A	Proteomics	10.1002/pmic.200700287	2007	*
Enzymology	Purification and characterization of fumarase from Corynebacterium glutamicum.	Genda T, Watabe S, Ozaki H	Biosci Biotechnol Biochem	10.1271/bbb.70.1102	2006	*
Enzymology	Purification and characterization of malate dehydrogenase from Corynebacterium glutamicum.	Genda T, Nakamatsu T, Ozak H	J Biosci Bioeng	S1389-1723(03)80162-7	2003	*
Metabolism	Enhanced glutamic acid production by a H+-ATPase-defective mutant of Corynebacterium glutamicum.	Aoki R, Wada M, Takesue N, Tanaka K, Yokota A	Biosci Biotechnol Biochem	10.1271/bbb.69.1466	2005	*
Metabolism	H+-ATPase defect in Corynebacterium glutamicum abolishes glutamic acid production with enhancement of glucose consumption rate.	Sekine H, Shimada T, Hayashi C, Ishiguro A, Tomita F, Yokota A	Appl Microbiol Biotechnol	10.1007/s002530100778	2001	*
Enzymology	Application of RecET-Cre/loxP system in Corynebacterium glutamicum ATCC14067 for L-leucine production.	Luo G, Zhao N, Jiang S, Zheng S	Biotechnol Lett	10.1007/s10529-020-03000-1	2020	*
Metabolism	Mechanism of increased respiration in an H+-ATPase-defective mutant of Corynebacterium glutamicum.	Sawada K, Kato Y, Imai K, Li L, Wada M, Matsushita K, Yokota A	J Biosci Bioeng	10.1016/j.jbiosc.2011.11.021	2011	*
Metabolism	Enhanced valine production in Corynebacterium glutamicum with defective H+-ATPase and C-terminal truncated acetohydroxyacid synthase.	Wada M, Hijikata N, Aoki R, Takesue N, Yokota A	Biosci Biotechnol Biochem	10.1271/bbb.80434	2008	*
Proteome	Cytoplasmic proteome reference map for a glutamic acid-producing Corynebacterium glutamicum ATCC 14067.	Li L, Wada M, Yokota A	Proteomics	10.1002/pmic.200700269	2007	*
Enzymology	The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032.	Hansmeier N, Albersmeier A, Tauch A, Damberg T, Ros R, Anselmetti D, Puhler A, Kalinowski J	Microbiology (Reading)	10.1099/mic.0.28673-0	2006	*
Metabolism	[Nicotinamide-adenine dinucleotide synthesis by microorganisms].	Kutseva LS, Bazdyreva NM, Ivanova LB	Prikl Biokhim Mikrobiol		1981	*
	Transcriptional Regulation of the Creatine Utilization Genes of Corynebacterium glutamicum ATCC 14067 by AmtR, a Central Nitrogen Regulator.	Zhang H, Ouyang Z, Zhao N, Han S, Zheng S	Front Bioeng Biotechnol	10.3389/fbioe.2022.816628	2022	*

References

#8797

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20411

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72612

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35961.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Corynebacterium glutamicum in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Corynebacterium glutamicum ATCC 14067

Corynebacterium glutamicum ATCC 14067 ATCC 14067

Change proposal

Change proposal