Strain identifier

BacDive ID: 3094

Type strain: No

Species: Corynebacterium glutamicum

Strain Designation: 2247

Strain history: K. Suzuki CNF 014 <-- AJ 1510 <-- ATCC 14067 <-- Ajinomoto Co., Inc., Japan; 2247.

NCBI tax ID(s): 1079988 (strain), 1718 (species)

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General

@ref: 8797

BacDive-ID: 3094

DSM-Number: 20411

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, amino acid production

description: Corynebacterium glutamicum 2247 is a mesophilic bacterium that produces amino acids.

NCBI tax id

NCBI tax idMatching level
1079988strain
1718species

strain history

@refhistory
8797<- H. Seiler <- ATCC <- Ajinomoto Co., Inc., 2247 (Brevibacterium flavum)
67770K. Suzuki CNF 014 <-- AJ 1510 <-- ATCC 14067 <-- Ajinomoto Co., Inc., Japan; 2247.

doi: 10.13145/bacdive3094.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium glutamicum
  • full scientific name: Corynebacterium glutamicum (Kinoshita et al. 1958) Abe et al. 1967 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Micrococcus glutamicus
    20215Brevibacterium divaricatum
    20215Corynebacterium lilium

@ref: 8797

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium glutamicum

full scientific name: Corynebacterium glutamicum (Kinoshita et al. 1958) Abe et al. 1967 emend. Nouioui et al. 2018

strain designation: 2247

type strain: no

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.291
69480100positive

multimedia

  • @ref: 8797
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_20411.jpg
  • caption: Medium 535a 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8797COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8797TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yeshttps://mediadive.dsmz.de/medium/535aName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
8797TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf

culture temp

@refgrowthtypetemperaturerange
8797positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no99
69480no99.899

compound production

@refcompound
8797L glutamic acid
8797glutamate synthase

murein

  • @ref: 8797
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
6777029985L-glutamateyes

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382alkaline phosphatase-3.1.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
8797--+/---+--------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
8797+-+-+---+-++/--++-+/-+/-++/-

Safety information

risk assessment

  • @ref: 8797
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Corynebacterium glutamicum ATCC 14067 16S ribosomal RNA gene, partial sequence
  • accession: AY017108
  • length: 633
  • database: ena
  • NCBI tax ID: 1079988

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium glutamicum ATCC 14067GCA_002243555completencbi1079988
66792Corynebacterium glutamicum ATCC 140671079988.5completepatric1079988
66792Corynebacterium glutamicum ATCC 140671079988.3wgspatric1079988
66792Corynebacterium glutamicum ATCC 140672848153583completeimg1079988
66792Corynebacterium glutamicum ATCC 140672531839299draftimg1079988
67770Corynebacterium glutamicum ATCC 14067GCA_000233355contigncbi1079988

GC content

@refGC-contentmethod
879754.0
6777054.1thermal denaturation, midpoint method (Tm)
6777056.8

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
flagellatedno97.356no
gram-positiveyes88.157no
anaerobicno99.186no
aerobicyes74.775no
halophileyes64.834no
spore-formingno90.652no
glucose-utilyes87.327yes
thermophileno97.643yes
motileno93.078no
glucose-fermentyes72.726yes

External links

@ref: 8797

culture collection no.: DSM 20411, ATCC 14067, DSM 1413, DSM 778, NCIB 9565, JCM 1308, BCRC 11383, CCM 3600, IAM 12433, IFO 12153, KCCM 11318, KCTC 1738, NBRC 12153, NCIMB 9565

straininfo link

  • @ref: 72612
  • straininfo: 35961

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Metabolism6459575[Nicotinamide-adenine dinucleotide synthesis by microorganisms].Kutseva LS, Bazdyreva NM, Ivanova LBPrikl Biokhim Mikrobiol1981Acetone/pharmacology, Adenine/metabolism, Adenosine Triphosphate/metabolism, Brevibacterium/*metabolism, Corynebacterium/*metabolism, Dose-Response Relationship, Drug, Fermentation/drug effects, Micrococcus/*metabolism, NAD/*biosynthesis, Niacinamide/metabolismPathogenicity
Metabolism11762601H+-ATPase defect in Corynebacterium glutamicum abolishes glutamic acid production with enhancement of glucose consumption rate.Sekine H, Shimada T, Hayashi C, Ishiguro A, Tomita F, Yokota AAppl Microbiol Biotechnol2001Corynebacterium/*metabolism, Dicyclohexylcarbodiimide/pharmacology, Ethanol/pharmacology, Glucose/*metabolism, Glutamic Acid/*metabolism, Operon, Proton-Translocating ATPases/*physiologyBiotechnology10.1007/s002530100778
Metabolism16116273Enhanced glutamic acid production by a H+-ATPase-defective mutant of Corynebacterium glutamicum.Aoki R, Wada M, Takesue N, Tanaka K, Yokota ABiosci Biotechnol Biochem2005Biotin/metabolism, Corynebacterium glutamicum/enzymology/genetics/growth & development/*metabolism, Fermentation, Glutamic Acid/*biosynthesis, Proton-Translocating ATPases/genetics/*metabolismBiotechnology10.1271/bbb.69.1466
Enzymology16233457Purification and characterization of malate dehydrogenase from Corynebacterium glutamicum.Genda T, Nakamatsu T, Ozak HJ Biosci Bioeng2003S1389-1723(03)80162-7
Enzymology16549657The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032.Hansmeier N, Albersmeier A, Tauch A, Damberg T, Ros R, Anselmetti D, Puhler A, Kalinowski JMicrobiology (Reading)2006Bacterial Proteins/*biosynthesis/*genetics, Corynebacterium glutamicum/*genetics/metabolism, DNA, Bacterial/chemistry/genetics, DNA-Binding Proteins/genetics/isolation & purification/physiology, Electrophoresis, Polyacrylamide Gel, *Gene Expression Regulation, Bacterial, Gene Library, *Genomic Islands, Mass Spectrometry, Microscopy, Atomic Force, Molecular Sequence Data, Open Reading Frames, Polymerase Chain Reaction, Promoter Regions, Genetic, Proteome/analysis, Recombination, Genetic, Repetitive Sequences, Nucleic Acid, Repressor Proteins/genetics/isolation & purification/*physiology, Sequence Analysis, DNA, Sequence Homology, Synteny, Terminal Repeat Sequences, Trans-Activators/genetics/isolation & purification/*physiology, *Transcription, GeneticPhylogeny10.1099/mic.0.28673-0
Enzymology16717409Purification and characterization of fumarase from Corynebacterium glutamicum.Genda T, Watabe S, Ozaki HBiosci Biotechnol Biochem2006Bacterial Proteins/antagonists & inhibitors/*chemistry/isolation & purification, Benzoates/chemistry, Corynebacterium glutamicum/*enzymology, Enzyme Stability, Fumarate Hydratase/antagonists & inhibitors/*chemistry/isolation & purification, Hydrogen-Ion Concentration, Molecular Weight, Protein Subunits/antagonists & inhibitors/chemistry/isolation & purification, Substrate Specificity, Sulfhydryl Compounds/chemistry, Tartrates/chemistryPhylogeny10.1271/bbb.70.1102
Proteome17849411A comparative proteomic approach to understand the adaptations of an H+ -ATPase-defective mutant of Corynebacterium glutamicum ATCC14067 to energy deficiencies.Li L, Wada M, Yokota AProteomics2007Citric Acid Cycle, Corynebacterium glutamicum/enzymology/*genetics/growth & development, Fermentation, Glycolysis, *Mutation, NAD/metabolism, *Proteome, Proton-Translocating ATPases/*geneticsEnzymology10.1002/pmic.200700287
Metabolism18040683Microbial production of L -glutamate and L -glutamine by recombinant Corynebacterium glutamicum harboring Vitreoscilla hemoglobin gene vgb.Liu Q, Zhang J, Wei XX, Ouyang SP, Wu Q, Chen GQAppl Microbiol Biotechnol2007Bacterial Proteins/*biosynthesis/*genetics, Biomass, Bioreactors, Corynebacterium glutamicum/*genetics/metabolism, Glutamic Acid/*metabolism, Glutamine/*metabolism, Kinetics, Mutagenesis, Site-Directed, Promoter Regions, Genetic, Protein Engineering/*methods, Recombinant Proteins/biosynthesis/genetics, Truncated Hemoglobins/*biosynthesis/*geneticsEnzymology10.1007/s00253-007-1254-8
Proteome18040983Cytoplasmic proteome reference map for a glutamic acid-producing Corynebacterium glutamicum ATCC 14067.Li L, Wada M, Yokota AProteomics2007Bacterial Proteins/*analysis, Corynebacterium glutamicum/*chemistry/metabolism, Cytoplasm/chemistry, Databases, Protein, Electrophoresis, Gel, Two-Dimensional, Glutamic Acid/*metabolism, Proteome/*analysis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationMetabolism10.1002/pmic.200700269
Metabolism18997402Enhanced valine production in Corynebacterium glutamicum with defective H+-ATPase and C-terminal truncated acetohydroxyacid synthase.Wada M, Hijikata N, Aoki R, Takesue N, Yokota ABiosci Biotechnol Biochem2008Acetolactate Synthase/*chemistry/*genetics/metabolism, Corynebacterium glutamicum/enzymology/*genetics/*metabolism, *Gene Deletion, Genetic Engineering, Ketol-Acid Reductoisomerase/genetics, Proton-Translocating ATPases/genetics/*metabolism, Valine/*biosynthesisBiotechnology10.1271/bbb.80434
Metabolism22188772Mechanism of increased respiration in an H+-ATPase-defective mutant of Corynebacterium glutamicum.Sawada K, Kato Y, Imai K, Li L, Wada M, Matsushita K, Yokota AJ Biosci Bioeng2011Corynebacterium glutamicum/enzymology/*genetics/*metabolism, Electron Transport/genetics, Fermentation, Gene Expression Regulation, Bacterial, Mutation, NAD/metabolism, Oxidoreductases/metabolism, Proton-Translocating ATPases/*geneticsEnzymology10.1016/j.jbiosc.2011.11.021
Genetics22247536Genome sequence of Corynebacterium glutamicum ATCC 14067, which provides insight into amino acid biosynthesis in coryneform bacteria.Lv Y, Liao J, Wu Z, Han S, Lin Y, Zheng SJ Bacteriol2012Amino Acids/*biosynthesis, Base Sequence, Corynebacterium/genetics/metabolism, Corynebacterium glutamicum/*genetics/*metabolism, *Genome, Bacterial, Molecular Sequence DataMetabolism10.1128/JB.06514-11
Enzymology25108235Construction of a novel expression system for use in Corynebacterium glutamicum.Hu J, Li Y, Zhang H, Tan Y, Wang XPlasmid2014Cloning, Molecular, Corynebacterium glutamicum/*genetics, DNA Copy Number Variations, DNA, Bacterial/genetics, Fermentation, Gene Deletion, *Gene Expression Regulation, Bacterial, Genes, Bacterial, Genetic Vectors/*genetics, Plasmids/*genetics, Valine/biosynthesis10.1016/j.plasmid.2014.07.005
Metabolism25750030Mutational analysis to identify the residues essential for the inhibition of N-acetyl glutamate kinase of Corynebacterium glutamicum.Huang Y, Zhang H, Tian H, Li C, Han S, Lin Y, Zheng SAppl Microbiol Biotechnol2015*Allosteric Regulation, Arginine/*metabolism, Binding Sites, Corynebacterium glutamicum/*enzymology, DNA Mutational Analysis, Enzyme Inhibitors/*metabolism, Molecular Docking Simulation, Mutagenesis, Site-Directed, Phosphotransferases (Carboxyl Group Acceptor)/*antagonists & inhibitors, Protein BindingEnzymology10.1007/s00253-015-6469-5
Metabolism28801604Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette.Huang Y, Li L, Xie S, Zhao N, Han S, Lin Y, Zheng SSci Rep2017Corynebacterium glutamicum/*genetics, Exodeoxyribonucleases/metabolism, Gene Knockout Techniques/*methods, Genetics, Microbial/*methods, Recombinases/metabolism10.1038/s41598-017-08352-9
Metabolism31307581The 138(th) residue of acetohydroxyacid synthase in Corynebacterium glutamicum is important for the substrate binding specificity.Liu Y, Wang X, Zhan J, Hu JEnzyme Microb Technol2019Acetolactate Synthase/*chemistry/genetics/metabolism, Amino Acid Motifs, Bacterial Proteins/*chemistry/genetics/metabolism, Butyrates/metabolism, Corynebacterium glutamicum/chemistry/*enzymology/genetics, Isoleucine/metabolism, Pyruvic Acid/metabolism, Substrate Specificity, Valine/metabolismEnzymology10.1016/j.enzmictec.2019.06.001
Enzymology32876764Multiplex gene editing and large DNA fragment deletion by the CRISPR/Cpf1-RecE/T system in Corynebacterium glutamicum.Zhao N, Li L, Luo G, Xie S, Lin Y, Han S, Huang Y, Zheng SJ Ind Microbiol Biotechnol2020CRISPR-Associated Proteins/*genetics, CRISPR-Cas Systems/*genetics, Clustered Regularly Interspaced Short Palindromic Repeats, Corynebacterium glutamicum/enzymology/*genetics/metabolism, DNA, Bacterial/*genetics, *Gene Deletion, Gene Editing/*methods, Metabolic Engineering/methodsMetabolism10.1007/s10295-020-02304-5
Enzymology32936374Application of RecET-Cre/loxP system in Corynebacterium glutamicum ATCC14067 for L-leucine production.Luo G, Zhao N, Jiang S, Zheng SBiotechnol Lett2020Cloning, Molecular, *Corynebacterium glutamicum/genetics/metabolism, Integrases/genetics, Leucine/*metabolism, Metabolic Engineering/*methods, Promoter Regions, Genetic/geneticsMetabolism10.1007/s10529-020-03000-1
35223787Transcriptional Regulation of the Creatine Utilization Genes of Corynebacterium glutamicum ATCC 14067 by AmtR, a Central Nitrogen Regulator.Zhang H, Ouyang Z, Zhao N, Han S, Zheng SFront Bioeng Biotechnol202210.3389/fbioe.2022.816628

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8797Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20411)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20411
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
72612Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35961.1StrainInfo: A central database for resolving microbial strain identifiers