Name: Comamonas nitrativorans 23310
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2929

Type strain:

Species: Comamonas nitrativorans

Strain Designation: 23310

Culture col. no.: DSM 13191, CCT 7062, NCCB 100007, CCUG 45781, CIP 107121

Strain history: CIP <- 2001, C. Etchebehere, Departamento de Microbiologia, Uruguay: strain 23310

NCBI tax ID(s): 1121350 (strain), 108437 (species)

SI-ID 49433

DSM 13191, CCT 7062, NCCB 100007, CCUG 45781, CIP 107121, LMG 20949

Section

Comamonas nitrativorans 23310 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from lab. scale denitrifying reactor treating landfill leachate.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Comamonas*
	Species	**Comamonas nitrativorans**
	Full Scientific Name (LPSN)	Comamonas nitrativorans Etchebehere et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #121308]	Gram stain	negative

[Ref.: #121308]	Cell shape	rod-shaped

[Ref.: #121308]	Motility	yes

[Ref.: #121308]	Pigment production	no
[Ref.: #121308]	Pigment name	Pyocyanin

Multimedia content

[Ref.: #4860]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4860]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #4860]

Culture medium growth

[Ref.: #4860]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4860]

Culture medium composition

expand / minimize

[Ref.: #4860]

Culture medium

BHI MEDIUM (DSMZ Medium 215)

[Ref.: #4860]

Culture medium growth

[Ref.: #4860]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4860]

Culture medium composition

expand / minimize

[Ref.: #39890]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #39890]

Culture medium growth

[Ref.: #39890]

Culture medium composition

expand / minimize

[Ref.: #121308]

Culture medium

CIP Medium 72

[Ref.: #121308]

Culture medium growth

[Ref.: #121308]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #4860]		growth	30 °C
[Ref.: #39890]		growth	30 °C
[Ref.: #121308]		growth	25-41 °C
[Ref.: #121308]	no	growth	5 °C
[Ref.: #121308]	no	growth	10 °C

[Ref.: #4860]	Temperature range	mesophilic
[Ref.: #39890]	Temperature range	mesophilic
[Ref.: #121308]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4860]	Oxygen tolerance	aerobe
[Ref.: #4860]	Oxygen tolerance	microaerophile
[Ref.: #121308]	Oxygen tolerance	obligate aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #121308]		NaCl		growth	0-10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #121308]	citrate ChEBI	-	carbon source
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	-	assimilation	* from API 20NE
[Ref.: #121308]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #121308]	nitrate ChEBI	+	reduction
[Ref.: #121308]	nitrate ChEBI	+	respiration
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #121308]	nitrite ChEBI	+	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121308]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #121308]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121308]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121308]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #121308]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121308]	caseinase	-	3.4.21.50
[Ref.: #121308]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #121308]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #121308]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #121308]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121308]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121308]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121308]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121308]	oxidase	+
[Ref.: #121308]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121308]	tryptophan deaminase	-
[Ref.: #121308]	tween esterase	-
[Ref.: #121308]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #56922]

Fatty acid	Percentage	ECL
C16:1 ω7c	50.8	15.819
C16:0	14.2	16
C18:1 ω7c /12t/9t	10.7	17.824
C10:0 3OH	10	11.423
C12:0	8.7	12
C14:0	5.6	14

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #4860]	23372	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121308]	2013	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	-	-	+	-	-	-	-	+	+	+	+	+	+	-	-	-	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121308]	10081	-	-	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4860]	Sample type/isolated from	lab. scale denitrifying reactor treating landfill leachate
[Ref.: #4860]	Geographic location (country and/or sea, region)	Montevideo
[Ref.: #4860]	Country	Uruguay
[Ref.: #4860]	Country ISO 3 Code	URY
[Ref.: #4860]	Continent	Middle and South America

[Ref.: #56922]	Sample type/isolated from	Landfill leachate,denitrifying reactor

[Ref.: #121308]	Sample type/isolated from	Denitrifying reactor treating landfill leachate
[Ref.: #121308]	Country	Uruguay
[Ref.: #121308]	Country ISO 3 Code	URY
[Ref.: #121308]	Continent	Middle and South America
[Ref.: #121308]	Isolation date	1998
* marker position based on {}

Isolation sources categories

#Engineered	#Bioreactor	-
#Engineered	#Waste	#Landfill
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ251577 (>99% sequence identity) for Comamonas nitrativorans subclade from Microbeatlas

Aquatic Samples	131
Soil Samples	14
Animal Samples	64
Plant Samples	7
Total Samples	216

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4860]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121308]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Comamonas sp. 16S rRNA gene, strain 23310	AJ251577	1498	ENA	108437 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Comamonas nitrativorans DSM 13191	2574180442	draft	JGI IMG/M	1121350 tax ID	*

Availability in culture collections External links

[Ref.: #4860]

Culture collection no.

DSM 13191, CCT 7062, NCCB 100007, CCUG 45781, CIP 107121

[Ref.: #72452]

SI-ID 49433

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Comamonas flocculans sp. nov., a Floc-Forming Bacterium Isolated from Livestock Wastewater.	Kim DH, Han KI, Kwon HJ, Kim MG, Kim YG, Choi DH, Lee KC, Suh MK, Kim HS, Lee JS, Kim JG	Curr Microbiol	10.1007/s00284-020-01940-5	2020	*
Phylogeny	Comamonas faecalis sp. nov., isolated from domestic pig feces.	Kim D, Lee SS	Curr Microbiol	10.1007/s00284-014-0561-4	2014	*
Phylogeny	Comamonas granuli sp. nov., isolated from granules used in a wastewater treatment plant.	Kim KH, Ten LN, Liu QM, Im WT, Lee ST	J Microbiol	10.1007/s12275-008-0019-0	2008	*
Phylogeny	Comamonas nitrativorans sp. nov., a novel denitrifier isolated from a denitrifying reactor treating landfill leachate.	Etchebehere C, Errazquin MI, Dabert P, Moletta R, Muxi L	Int J Syst Evol Microbiol	10.1099/00207713-51-3-977	2001	*

References

#4860

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13191

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39890 ; Curators of the CIP;
#56922 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45781

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#72452

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49433.1 )

#121308 Collection of Institut Pasteur ; Curators of the CIP; CIP 107121
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Comamonas nitrativorans in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF