Acidovorax delafieldii (CCUG 1779, LMG 5943, DSM 64)

Name: Acidovorax delafieldii (CCUG 1779, LMG 5943, DSM 64)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2885

Type strain

Strain Designation 133, FD-6

Culture col. no. CCUG 1779 LMG 5943 DSM 64 ATCC 17505 DSM 50263 5 more

NCBI tax ID(s) 47920

Special Collections DSMZ CCUG CIP

History <- IMG <- R.Y. Stanier, 133 <- F. Delafield, FD-6 CIP <- 1989, DSM <- Inst. Mikrobiol. Univ., Göttinger, Germany <- R.Y. Stanier, Inst. Pasteur, Paris, France: strain 133, Pseudomonas delafieldii <- F. Delafield: strain FD-6

Links

Acidovorax delafieldii 133 is an obligate aerobe, Gram-negative, motile bacterium that was isolated from soil, enrichment with poly-ß-hydroxybutyrate.

Gram-negative motile rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 92080

LMG 5943,ATCC 17505,CCUG 1779,DSM 50263,DSM 50403,DSM 64,LMG 6832,NCIB 11476,CIP 103303,NCIMB 11476,CCM 3589,LMD 90.17,CECT 4105,CCRC 13888,CFBP 2442,NCCB 90017,BCRC 13888,CFBP 3609,CGMCC 1.2784

@ref 20215

Last LPSN update 2026-02-09 11:18:22

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Comamonadaceae

Genus Acidovorax

Species Acidovorax delafieldii

Full scientific name Acidovorax delafieldii (Davis 1970) Willems et al. 1990

Synonyms (1)

Pseudomonas delafieldii

BacDive ID	Other strains from **Acidovorax delafieldii** (12)	Type strain
2884	A. delafieldii FD-7, DSM 4762, ATCC 17506, CCUG 14277, LMG ...
136207	A. delafieldii CIP 103454, CCUG 16516, LMG 8905
136209	A. delafieldii CIP 103455, CCUG 18251, LMG 7186
138350	A. delafieldii CIP 106437, CCUG 11056, LMG 7166
138351	A. delafieldii CIP 106438, CCUG 11062, LMG 7168
142545	A. delafieldii CCUG 12929, LMG 7167
142758	A. delafieldii CCUG 14478, ATCC 17508, LMG 5944
143051	A. delafieldii CCUG 16566, LMG 8906
143556	A. delafieldii CCUG 20074, LMG 7679
143828	A. delafieldii CCUG 21358, LMG 8916
144333	A. delafieldii CCUG 24594, LMG 9090
157283	A. delafieldii IMI 359867

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
118877	negative	rod-shaped

Pigmentation

@ref	Production	Name
118877		Pyocyanin

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2047	REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)	Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
2047	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
40594	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
118877	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
2047	positive	growth	30
40594	positive	growth	25
118877	positive	growth	5-37
118877	negative	growth	41

Physiology and metabolism

Oxygen tolerance

118877

Oxygen toleranceobligate aerobe

Halophily

@ref	Salt	Growth	Tested relation	Concentration
118877	NaCl	positive	growth	0-2 %
118877	NaCl		growth	4 %
118877	NaCl		growth	6 %
118877	NaCl		growth	8 %
118877	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
118877	16947 ChEBI	citrate	-	carbon source
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
118877	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
118877	17632 ChEBI	nitrate	+	reduction
118877	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
118877	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
118877	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
118877	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
118877	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
118877	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
118877	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
118877	caseinase	+	3.4.21.50
118877	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
118877	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
118877	gelatinase	-
68369	gelatinase	-		from API 20NE
118877	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
118877	lipase	+
68382	lipase (C 14)	-		from API zym
118877	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
118877	ornithine decarboxylase	-	4.1.1.17
118877	oxidase	+
118877	protease	+
68382	trypsin	-	3.4.21.4	from API zym
118877	tryptophan deaminase	-
118877	tween esterase	+
118877	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

44419

Fatty acid	Percentage	ECL
C16:1 ω7c	47	15.819
C16:0	21.8	16
C18:1 ω7c /12t/9t	15.4	17.824
C14:0	4.5	14
C12:0	3.9	12
C10:0 3OH	3.8	11.423
C18:2 ω6,9c/C18:0 ANTE	2.8	17.724
C8:0 3OH	0.9	9.385

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
44419	-	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
118877	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
2047	+	-	-	-	+	-	-	-	-	+	+	+	-	-	+	-	-	+	-	-	+
44419	+	-	-	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+
44419	+	-	+	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+
2047	+	-	-	-	-	-	-	-	+	+	+	+	-	-	+	-	-	+	-	-	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
118877	not determinedn.d.	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	+	-	-	-	-	+	-	+	-	-	+	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	+	-	-	-	+	-	+	+	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	+	+	-	-	-	-	-	+	-	+	+	+	+	-	+	+	-	-	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
2047	soil, enrichment with poly-ß-hydroxybutyrate
44419	Soil,enrichment with PHB as sole carbon source
118877	Environment, Soil enriched with poly-b-hydroxybuty

Taxonmaps

69479

Global distribution of 16S sequence AF078764 (>99% sequence identity) for Acidovorax from Microbeatlas

Aquatic Samples	155
Soil Samples	75
Animal Samples	38
Plant Samples	904
Total Samples	1172

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
2047	yes, in single cases	1	Risk group (German classification) According to TRBA 466
118877		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM799381v1 assembly for Acidovorax delafieldii DSM 64	scaffold	GCA_007993815	47920.27	2636416192	47920	75.19

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Pseudomonas putida strain 133 16S ribosomal RNA gene, partial sequence	JQ795146	403	303
20218	Acidovorax delafieldii 16S ribosomal RNA gene, partial sequence	AF078764	1515	47920
20218	Acidovorax delafieldii 16S rRNA gene, strain DSM 50263	AJ420323	1483	47920
20218	Acidovorax delafieldii strain LMG 5943 16S ribosomal RNA gene, partial sequence	EU024132	1318	47920

GC content

2047

GC-content (mol%)64.8

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Biostimulation and microbial community profiling reveal insights on RDX transformation in groundwater.	Wang D, Boukhalfa H, Marina O, Ware DS, Goering TJ, Sun F, Daligault HE, Lo CC, Vuyisich M, Starkenburg SR.	Microbiologyopen	10.1002/mbo3.423	2017
Metabolism	Aerobic and oxygen-limited naphthalene-amended enrichments induced the dominance of Pseudomonas spp. from a groundwater bacterial biofilm.	Benedek T, Szentgyorgyi F, Szabo I, Farkas M, Duran R, Kriszt B, Tancsics A.	Appl Microbiol Biotechnol	10.1007/s00253-020-10668-y	2020
Metabolism	The major outer membrane protein of Acidovorax delafieldii is an anion-selective porin.	Brunen M, Engelhardt H, Schmid A, Benz R.	J Bacteriol	10.1128/jb.173.13.4182-4187.1991	1991
Genetics	Discovery of Antimicrobial Phosphonopeptide Natural Products from Bacillus velezensis by Genome Mining.	Wilson J, Cui J, Nakao T, Kwok H, Zhang Y, Kayrouz CM, Pham TM, Roodhouse H, Ju KS.	Appl Environ Microbiol	10.1128/aem.00338-23	2023
	A persistent and diverse airway microbiota present during chronic obstructive pulmonary disease exacerbations.	Huang YJ, Kim E, Cox MJ, Brodie EL, Brown R, Wiener-Kronish JP, Lynch SV.	OMICS	10.1089/omi.2009.0100	2010
Metabolism	Regulation of pyrimidine biosynthesis in Acidovorax delafieldii.	West TP	J Basic Microbiol	10.1002/jobm.200410506	2005
	Acidovorax benzenivorans sp. nov., a novel aromatic hydrocarbon-degrading bacterium isolated from a xylene-degrading enrichment culture.	Bedics A, Tancsics A, Banerjee S, Toth E, Harkai P, Gottschall GG, Boka K, Kriszt B.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006219	2024
Phylogeny	Acidovorax kalamii sp. nov., isolated from a water sample of the river Ganges.	Pal D, Kaur N, Sudan SK, Bisht B, Krishnamurthi S, Mayilraj S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002736	2018
Phylogeny	Acidovorax, a new genus for Pseudomonas facilis, Pseudomonas delafieldii, E. Falsen (EF) group 13, EF group 16, and several clinical isolates, with the species Acidovorax facilis comb. nov., Acidovorax delafieldii comb. nov., and Acidovorax temperans sp. nov.	Willems A, Falsen E, Pot B, Jantzen E, Hoste B, Vandamme P, Gillis M, Kersters K, De Ley J	Int J Syst Bacteriol	10.1099/00207713-40-4-384	1990

References

#2047	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 64
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40594	; Curators of the CIP;
#44419	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 1779
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118877	Collection of Institut Pasteur ; Curators of the CIP; CIP 103303
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acidovorax delafieldii (CCUG 1779, LMG 5943, DSM 64)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_007993815

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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