Name: Clostridium laramiense NK1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2807

Type strain:

Species: Clostridium laramiense

Strain Designation: NK1

Culture col. no.: DSM 14864, ATCC 51254

Strain history: <- ATCC <- B. Ray, University of Wyoming, Laramie, USA; NK1 <- N. Kalchayanand {1989}

NCBI tax ID(s): 205326 (subspecies)

SI-ID 265711

ATCC 51254, DSM 14864

Section

Clostridium laramiense NK1 is an anaerobe, spore-forming, psychrophilic bacterium that was isolated from spoiled umcooked beef.

motile
spore-forming
anaerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	Clostridium laramiense
	Full Scientific Name (LPSN)	Clostridium laramiense corrig. Kalchayanand et al. 1993

	Synonym	Clostridium laramie
	Synonym	**Clostridium estertheticum subsp. laramiense**

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %93.092

[Ref.: #5584]

Incubation period

>14 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5584]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)

[Ref.: #5584]

Culture medium growth

[Ref.: #5584]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5584]

Culture medium composition

expand / minimize

[Ref.: #5584]

Culture medium

CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)

[Ref.: #5584]

Culture medium growth

[Ref.: #5584]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5584]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #5584]			growth	10 °C

[Ref.: #5584]

Temperature range

psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #5584]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %99.491

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5584]	Sample type/isolated from	spoiled umcooked beef
[Ref.: #5584]	Country	USA
[Ref.: #5584]	Country ISO 3 Code	USA
[Ref.: #5584]	Continent	North America
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Meat
#Host	#Other	#Decomposing animal

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ506115 (>99% sequence identity) for Clostridium from Microbeatlas

Aquatic Samples	5428
Soil Samples	9246
Animal Samples	1548
Plant Samples	2245
Total Samples	18467

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5584]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5584]	Clostridium estertheticum subsp. laramiense 16S rRNA gene, strain DSM 14864	AJ506115	1473	ENA	205326 tax ID
[Ref.: #20218]	Clostridium estertheticum subsp. laramiense strain DSM 14864 16S ribosomal RNA gene, partial sequence	DQ911251	327	ENA	205326 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Clostridium estertheticum subsp. laramiense DSM 14864	GCA_008933175	contig	GenBank	205326 tax ID	*
[Ref.: #66792]	Clostridium estertheticum subsp. laramiense strain DSM 14864	205326.4	wgs	PATRIC	205326 tax ID	*
[Ref.: #66792]	Clostridium estertheticum laramiense DSM 14864	2860493848	draft	JGI IMG/M	205326 tax ID	*

[Ref.: #5584]	GC-content	32 mol%	high performance liquid chromatography (HPLC)
[Ref.: #5584]	GC-content	26 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Clostridium estertheticum subsp. laramiense strain DSM 14864 PATRIC: 205326.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Clostridium estertheticum subsp. laramiense strain DSM 14864 PATRIC: 205326.4
[Ref.: #69480]	motile	yes	81.979	no
[Ref.: #69480]	gram-positive	yes	69.701	no
[Ref.: #69480]	anaerobic	yes	95.946	no
[Ref.: #69480]	aerobic	no	96.585	yes
[Ref.: #69480]	halophile	no	96.821	no
[Ref.: #69480]	spore-forming	yes	94.251	no
[Ref.: #69480]	glucose-util	yes	91.943	no
[Ref.: #69480]	flagellated	yes	58.507	no
[Ref.: #69480]	thermophile	no	98.847	yes
[Ref.: #69480]	glucose-ferment	yes	66.958	no

Availability in culture collections External links

[Ref.: #5584]

Culture collection no.

DSM 14864, ATCC 51254

[Ref.: #72333]

SI-ID 265711

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov.	Spring S, Merkhoffer B, Weiss N, Kroppenstedt RM, Hippe H, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.02554-0	2003	*

References

#5584

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14864

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72333

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID265711.1 )

* These data were automatically processed and therefore are not curated

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Clostridium estertheticum subsp. laramiense strain DSM 14864

Clostridium estertheticum subsp. laramiense strain DSM 14864

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