Name: Clostridium tetani E4222
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2791

Type strain:

Species: Clostridium tetani

Strain Designation: E4222

Culture col. no.: DSM 11745

Strain history: <- G. Schallehn, E4222

NCBI tax ID(s): 1513 (species)

SI-ID 49166

DSM 11745

Other strains from Clostridium tetani

C. tetani CCUG 4220, ATCC 19406, NCTC 279 (type strain)
C. tetani 34946A, DSM 11744
C. tetani 407-86, CIP 108174
C. tetani CIP 108172
C. tetani 358-99, CIP 108173
C. tetani 132CV, CIP 108175
C. tetani CCUG 1408
C. tetani CCUG 52330
C. tetani Harvard, CIP 60.37

Section

Clostridium tetani E4222 is an anaerobe, mesophilic human pathogen that was isolated from human clinical specimens.

anaerobe
human pathogen
mesophilic
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Clostridium*
	Species	**Clostridium tetani**
	Full Scientific Name (LPSN)	Clostridium tetani (Flügge 1886) Bergey et al. 1923 (Approved Lists 1980)

Synonym

"Bacillus tetani"

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4470]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

FASTIDIOUS ANAEROBE BROTH (DSMZ Medium 1203a)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #4470]

Culture medium composition

expand / minimize

[Ref.: #4470]

Culture medium

FASTIDIOUS ANAEROBE AGAR (DSMZ Medium 1203)

[Ref.: #4470]

Culture medium growth

[Ref.: #4470]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4470]

Culture medium composition

expand / minimize

[Ref.: #4470]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #4470]

Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #4470]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #4470]			growth	37 °C

[Ref.: #4470]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4470]

Oxygen tolerance

anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68367]	cellobiose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-glucose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-mannose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	D-xylose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	esculin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #68367]	gelatin ChEBI	+	hydrolysis	* from API 20A
[Ref.: #68367]	glycerol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-arabinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	L-rhamnose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	lactose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	maltose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	melezitose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	raffinose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	salicin ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sorbitol ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	sucrose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	trehalose ChEBI	-	builds acid from	* from API 20A
[Ref.: #68367]	tryptophan ChEBI	-	energy source	* from API 20A
[Ref.: #68367]	urea ChEBI	-	hydrolysis	* from API 20A
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68367]		indole ChEBI	no	* from API 20A

	Physiological tests	Metabolite	Indole test
[Ref.: #68367]		indole ChEBI	-	* from API 20A

	Enzyme	Enzyme activity	EC number
[Ref.: #68367]	beta-glucosidase	+	3.2.1.21	* from API 20A
[Ref.: #68367]	gelatinase	+		* from API 20A
[Ref.: #68367]	urease	-	3.5.1.5	* from API 20A

API® 20A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Indole productionIND	Urease/urea hydrolysisURE	Acid from D-glucoseGLU	Acid from D-mannitolMAN	Acid from lactoseLAC	Acid from sucroseSAC	Acid from maltoseMAL	Acid from salicinSAL	Acid from D-xyloseXYL	Acid from L-arabinoseARA	Gelatinase/gelatin hydrolysisGEL	Esculin hydrolysis/beta-glucosidaseESC	Acid from glycerolGLY	Acid from cellobioseCEL	Acid from D-mannoseMNE	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from sorbitolSOR	Acid from L-rhamnoseRHA	Acid from trehaloseTRE	CatalaseCAT	Spores presentSPOR	GramGRAM	Morphology coccus="+" rod="-"COCC
[Ref.: #4470]	2204	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4470]	Sample type/isolated from	human clinical specimens
* marker position based on {}

Isolation sources categories

#Infection	#Medical environment	#Clinic
#Infection	#Patient	#Specimen
#Host	#Human	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4470]	Pathogenicity (human)	yes
[Ref.: #4470]	Pathogenicity (animal)	yes
[Ref.: #4470]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

[Ref.: #4470]

GC-content

26 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #4470]

Culture collection no.

DSM 11745

[Ref.: #72318]

SI-ID 49166

References

#4470

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11745

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#68367

Automatically annotated from API 20A .

#72318

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49166.1 )

* These data were automatically processed and therefore are not curated

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