Name: Hathewaya proteolytica CG
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2648

Type strain:

Species: Hathewaya proteolytica

Strain Designation: CG

Culture col. no.: DSM 3090, ATCC 49002

Strain history: <- M.K. Jain and J.G. Zeikus, CG

NCBI tax ID(s): 1121331 (strain), 29365 (species)

SI-ID 42239

ATCC 49002, DSM 3090, BCRC 14533, CCRC 14533

Section

Hathewaya proteolytica CG is an anaerobe, spore-forming, mesophilic bacterium that forms circular colonies and was isolated from chicken manure digester.

colony-forming
Gram-positive
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Clostridiaceae*
	Genus	*Hathewaya*
	Species	**Hathewaya proteolytica**
	Full Scientific Name (LPSN)	Hathewaya proteolytica (Jain and Zeikus 1988) Lawson and Rainey 2016

Synonym

Clostridium proteolyticum

Information on morphological and physiological properties Morphology

[Ref.: #43779]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43779]	Cell length	2.2 µm

[Ref.: #43779]	Cell width	0.5 µm

[Ref.: #43779]	Cell shape	rod-shaped

[Ref.: #43779]	Motility	no

[Ref.: #43779]	Type of hemolysis	gamma
[Ref.: #43779]	Colony size	1.5 mm
[Ref.: #43779]	Colony color	Off-white
[Ref.: #43779]	Colony shape	circular

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1296]

Culture medium

WILKINS-CHALGREN ANAEROBE BROTH (DSMZ Medium 339)

[Ref.: #1296]

Culture medium growth

[Ref.: #1296]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1296]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #1296]			growth	37 °C
[Ref.: #43779]			optimum	30-37 °C

[Ref.: #1296]	Temperature range	mesophilic
[Ref.: #43779]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43779]			optimum	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #1296]	Oxygen tolerance	anaerobe
[Ref.: #43779]	Oxygen tolerance	obligate anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #43779]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %94.596

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43779]	casein	+/-	growth
[Ref.: #43779]	collagen ChEBI	+	assimilation
[Ref.: #43779]	gelatin ChEBI	+	assimilation
[Ref.: #43779]	meat extract	+	assimilation
[Ref.: #43779]	peptone	+	assimilation

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #43779]		penicillin ChEBI	yes	100	µg/mL
[Ref.: #43779]		streptomycin ChEBI	yes	100	µg/mL

	Metabolite	Production
[Ref.: #43779]	dihydrogen ChEBI	yes
[Ref.: #43779]	ethanol ChEBI	yes
[Ref.: #43779]	isobutyrate ChEBI	yes
[Ref.: #43779]	isovalerate ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43779]		catalase	-	1.11.1.6
[Ref.: #43779]		cytochrome oxidase	-	1.9.3.1

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	NAD metabolism	88.89	16 of 18
[Ref.: #66794]	vitamin B1 metabolism	84.62	11 of 13
[Ref.: #66794]	selenocysteine biosynthesis	83.33	5 of 6
[Ref.: #66794]	vitamin B12 metabolism	82.35	28 of 34
[Ref.: #66794]	lipoate biosynthesis	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	photosynthesis	78.57	11 of 14
[Ref.: #66794]	heme metabolism	78.57	11 of 14
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	biotin biosynthesis	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	pyrimidine metabolism	73.33	33 of 45
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	purine metabolism	70.21	66 of 94
[Ref.: #66794]	phenylalanine metabolism	69.23	9 of 13
[Ref.: #66794]	d-mannose degradation	66.67	6 of 9
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	glutamate and glutamine metabolism	60.71	17 of 28
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	threonine metabolism	60	6 of 10
[Ref.: #66794]	oxidative phosphorylation	59.34	54 of 91
[Ref.: #66794]	histidine metabolism	58.62	17 of 29
[Ref.: #66794]	reductive acetyl coenzyme A pathway	57.14	4 of 7
[Ref.: #66794]	serine metabolism	55.56	5 of 9
[Ref.: #66794]	proline metabolism	54.55	6 of 11
[Ref.: #66794]	methionine metabolism	53.85	14 of 26
[Ref.: #66794]	gluconeogenesis	50	4 of 8
[Ref.: #66794]	non-pathway related	50	19 of 38
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	starch degradation	50	5 of 10
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	d-xylose degradation	45.45	5 of 11
[Ref.: #66794]	vitamin B6 metabolism	45.45	5 of 11
[Ref.: #66794]	tryptophan metabolism	44.74	17 of 38
[Ref.: #66794]	CO2 fixation in Crenarchaeota	44.44	4 of 9
[Ref.: #66794]	degradation of sugar alcohols	43.75	7 of 16
[Ref.: #66794]	tetrahydrofolate metabolism	42.86	6 of 14
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	ubiquinone biosynthesis	42.86	3 of 7
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	isoprenoid biosynthesis	42.31	11 of 26
[Ref.: #66794]	lipid metabolism	41.94	13 of 31
[Ref.: #66794]	alanine metabolism	41.38	12 of 29
[Ref.: #66794]	ethylmalonyl-CoA pathway	40	2 of 5
[Ref.: #66794]	methylglyoxal degradation	40	2 of 5
[Ref.: #66794]	factor 420 biosynthesis	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	glycogen metabolism	40	2 of 5
[Ref.: #66794]	coenzyme M biosynthesis	40	4 of 10
[Ref.: #66794]	cysteine metabolism	38.89	7 of 18
[Ref.: #66794]	sulfate reduction	38.46	5 of 13
[Ref.: #66794]	lysine metabolism	38.1	16 of 42
[Ref.: #66794]	isoleucine metabolism	37.5	3 of 8
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	pentose phosphate pathway	36.36	4 of 11
[Ref.: #66794]	citric acid cycle	35.71	5 of 14
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	molybdenum cofactor biosynthesis	33.33	3 of 9
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	valine metabolism	33.33	3 of 9
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	1,4-dihydroxy-6-naphthoate biosynthesis	33.33	2 of 6
[Ref.: #66794]	nitrate assimilation	33.33	3 of 9
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	degradation of hexoses	33.33	6 of 18
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	leucine metabolism	30.77	4 of 13
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	arginine metabolism	29.17	7 of 24
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	polyamine pathway	26.09	6 of 23
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	23.08	3 of 13
[Ref.: #66794]	phenylpropanoid biosynthesis	23.08	3 of 13
[Ref.: #66794]	urea cycle	23.08	3 of 13
[Ref.: #66794]	degradation of pentoses	21.43	6 of 28
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1296]	Sample type/isolated from	chicken manure digester
[Ref.: #1296]	Geographic location (country and/or sea, region)	Wisconsin, Madison
[Ref.: #1296]	Country	USA
[Ref.: #1296]	Country ISO 3 Code	USA
[Ref.: #1296]	Continent	North America

[Ref.: #43779]	Sample type/isolated from	Chicken manure
* marker position based on {}

Isolation sources categories

#Host	#Birds	#Chicken
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1296]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1296]	Clostridium proteolyticum 16S rRNA gene, strain DSM 3090	X73448	1506	ENA	29365 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Hathewaya proteolytica DSM 3090	GCA_900142225	scaffold	GenBank	1121331 tax ID	*
[Ref.: #66792]	Clostridium proteolyticum DSM 3090	1121331.3	wgs	PATRIC	1121331 tax ID	*
[Ref.: #66792]	Hathewaya proteolytica DSM 3090	2585428174	draft	JGI IMG/M	1121331 tax ID	*

[Ref.: #1296]

GC-content

29.5 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Hathewaya proteolytica DSM 3090 DSM 3090 NCBI: GCA_900142225.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Hathewaya proteolytica DSM 3090 DSM 3090 NCBI: GCA_900142225.1
[Ref.: #69480]	motile	yes	76.963	no
[Ref.: #69480]	flagellated	no	65.376	yes
[Ref.: #69480]	gram-positive	yes	91.876	yes
[Ref.: #69480]	anaerobic	yes	99.12	yes
[Ref.: #69480]	aerobic	no	96.892	yes
[Ref.: #69480]	halophile	no	88.953	no
[Ref.: #69480]	spore-forming	yes	96.006	yes
[Ref.: #69480]	thermophile	no	99.552	yes
[Ref.: #69480]	glucose-util	yes	76.003	no
[Ref.: #69480]	glucose-ferment	yes	56.547	no

Availability in culture collections External links

[Ref.: #1296]

Culture collection no.

DSM 3090, ATCC 49002

[Ref.: #72179]

SI-ID 42239

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Oceanirhabdus sediminicola gen. nov., sp. nov., an anaerobic bacterium isolated from sea sediment.	Pi RX, Zhang WW, Fang MX, Zhang YZ, Li TT, Wu M, Zhu XF	Int J Syst Evol Microbiol	10.1099/ijs.0.051243-0	2013	*

References

#1296

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3090

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43779

Paul A. Lawson, Fred A. Rainey: Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related species. IJSEM 66: 1009 - 1016 2016 ( DOI 10.1099/ijsem.0.000824 , PubMed 26643615 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72179

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID42239.1 )

* These data were automatically processed and therefore are not curated

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Hathewaya proteolytica DSM 3090 DSM 3090

Hathewaya proteolytica DSM 3090 DSM 3090

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