Name: Pseudonocardia kujensis A4038
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 24450

Type strain:

Species: Pseudonocardia kujensis

Strain Designation: A4038, 5DSM 45670, 5NRRL B-24890

Culture col. no.: DSM 45670, KCTC 29062, NRRL B-24890

Strain history: <- Nevzat Sahin, Ondokuz Mayis Univ., Turkey

NCBI tax ID(s): 1128675 (species)

SI-ID 404428

DSM 45670

Special Collections

Section

Pseudonocardia kujensis A4038 is an aerobe, Gram-positive bacterium that builds an aerial mycelium and was isolated from soil.

Gram-positive
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Pseudonocardiales*
	Family	*Pseudonocardiaceae*
	Genus	*Pseudonocardia*
	Species	**Pseudonocardia kujensis**
	Full Scientific Name (LPSN)	Pseudonocardia kujensis Sahin et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	positive

[Ref.: #69419]	Colony color	Brown beige (1011)
[Ref.: #69419]	Cultivation medium used	ISP 6

[Ref.: #69419]	Colony color	Brown beige (1011)
[Ref.: #69419]	Cultivation medium used	suter with tyrosine

[Ref.: #69419]	Colony color	Brown beige (1011)
[Ref.: #69419]	Cultivation medium used	suter without tyrosine

[Ref.: #69419]	Colony color	Ochre brown (8001)
[Ref.: #69419]	Cultivation medium used	ISP 2

[Ref.: #69419]	Colony color	Ochre brwon (8001)
[Ref.: #69419]	Cultivation medium used	ISP 4

[Ref.: #69419]	Colony color	Sand yellow (1002)
[Ref.: #69419]	Cultivation medium used	ISP 3

[Ref.: #69419]	Colony color	Sand yellow (1002)
[Ref.: #69419]	Cultivation medium used	ISP 5

[Ref.: #69419]	Colony color	Sand yellow (1002)
[Ref.: #69419]	Cultivation medium used	ISP 7

[Ref.: #69419]	Pigment production	no
[Ref.: #69419]	Pigment name	soluble pigment

[Ref.: #69419]	Multicellular complex forming ability	no
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Medium Name (multicellularity)	ISP 2

[Ref.: #69419]	Multicellular complex forming ability	yes
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Multicellular complex color	Cream (9001)
[Ref.: #69419]	Medium Name (multicellularity)	ISP 3

[Ref.: #69419]	Multicellular complex forming ability	no
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Medium Name (multicellularity)	ISP 4

[Ref.: #69419]	Multicellular complex forming ability	yes
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Multicellular complex color	Spars, cream (9001)
[Ref.: #69419]	Medium Name (multicellularity)	ISP 5

[Ref.: #69419]	Multicellular complex forming ability	no
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Medium Name (multicellularity)	ISP 6

[Ref.: #69419]	Multicellular complex forming ability	no
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Medium Name (multicellularity)	ISP 7

[Ref.: #69419]	Multicellular complex forming ability	no
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69419]	Multicellular complex forming ability	no
[Ref.: #69419]	Multicellular complex name	Aerial mycelium
[Ref.: #69419]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #69419]

Caption:

Plates (553, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69419]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69419]

Caption:

Plates (553, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69419]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20752]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #20752]

Culture medium growth

[Ref.: #20752]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20752]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #20752]			growth	28 °C
[Ref.: #67771]			growth	28 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69419]		NaCl		growth	0-5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69419]	arabinose ChEBI	-	growth
[Ref.: #69419]	cellulose ChEBI	-	growth
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69419]	fructose ChEBI	+/-	growth
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69419]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69419]	inositol ChEBI	+	growth
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69419]	mannose ChEBI	-	growth
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69419]	raffinose ChEBI	-	growth
[Ref.: #69419]	rhamnose ChEBI	+/-	growth
[Ref.: #69419]	sucrose ChEBI	+/-	growth
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69419]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	-	3.2.1.20	* from API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne, API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne, API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69419]	3912	-	-	-	+/-	-	-	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69419]	6096	not determinedn.d.	-	+/-	-	-	+	+	+/-	+/-	-	+	+	-	-	-	+/-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20752]	Sample type/isolated from	soil
[Ref.: #20752]	Geographic location (country and/or sea, region)	Abuja, Kuje, Jabi
[Ref.: #20752]	Country	Nigeria
[Ref.: #20752]	Country ISO 3 Code	NGA
[Ref.: #20752]	Continent	Africa

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Arid estate, Abuja city
[Ref.: #67771]	Country	Nigeria
[Ref.: #67771]	Country ISO 3 Code	NGA
[Ref.: #67771]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JN989287 (>99% sequence identity) for Pseudonocardia kujensis from Microbeatlas

Aquatic Samples	16
Soil Samples	155
Animal Samples	23
Plant Samples	21
Total Samples	215

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20752]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20752]	Pseudonocardia kujensis strain A4038 16S ribosomal RNA gene, partial sequence	JN989287	1465	GenBank ENA	1128675 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Pseudonocardia kujensis KCTC 29062	GCA_021283305	contig	GenBank ENA	1128675 tax ID	*

[Ref.: #20752]

GC-content

71.9 mol%

fluorimetric

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Pseudonocardia kujensis KCTC 29062 NCBI: GCA_021283305.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.596	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.646	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	88.277	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	71.657	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.45	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	88.471	no

Availability in culture collections External links

[Ref.: #20752]

Culture collection no.

DSM 45670, KCTC 29062, NRRL B-24890

[Ref.: #88327]

SI-ID 404428

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Pseudonocardia thailandensis sp. nov., an actinomycete isolated from a subterranean termite nest.	Sujarit K, Sujada N, Kudo T, Ohkuma M, Pathom-Aree W, Lumyong S	Int J Syst Evol Microbiol	10.1099/ijsem.0.002017	2017	*
Phylogeny	Pseudonocardia terrae sp. nov., an actinobacterium isolated from rice rhizosphere soil in Thailand.	Muangham S, Duangmal K	Int J Syst Evol Microbiol	10.1099/ijsem.0.005430	2022	*
Phylogeny	Pseudonocardia cypriaca sp. nov., Pseudonocardia salamisensis sp. nov., Pseudonocardia hierapolitana sp. nov. and Pseudonocardia kujensis sp. nov., isolated from soil.	Sahin N, Veyisoglu A, Tatar D, Sproer C, Cetin D, Guven K, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijs.0.059824-0	2014	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20752

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45670

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69419

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88327

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID404428.1 )

* These data were automatically processed and therefore are not curated

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Pseudonocardia kujensis KCTC 29062

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