Name: Methanospirillum stamsii ps
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 24171

Type strain:

Species: Methanospirillum stamsii

Strain Designation: ps, Pt1

Culture col. no.: DSM 26304, VKM B-2808

Strain history: <- S. N. Parshina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; Pt1 <- S. N. Parshina; ps {2011}

NCBI tax ID(s): 1277351 (species)

SI-ID 398829

DSM 26304

Special Collections

Section

When using BacDive for research please cite our paper

Methanospirillum stamsii ps is an anaerobe, mesophilic, Gram-negative archaeon that was isolated from granulated biomass of an anaerobic expanded granular sludge bed bioreactor operated at low temperature.

Gram-negative
motile
rod-shaped
anaerobe
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Archaea*
	Phylum	*Methanobacteriota*
	Class	"Methanomicrobia"
	Order	*Methanomicrobiales*
	Family	*Methanospirillaceae*
	Genus	*Methanospirillum*
	Species	**Methanospirillum stamsii**
	Full Scientific Name (LPSN)	Methanospirillum stamsii Parshina et al. 2014

Information on morphological and physiological properties Morphology

[Ref.: #31177]	Gram stain	negative

[Ref.: #31177]	Cell length	7-25 µm

[Ref.: #31177]	Cell width	0.4-0.5 µm

[Ref.: #31177]	Cell shape	rod-shaped

[Ref.: #31177]	Motility	yes

[Ref.: #21024]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #21024]

Culture medium

METHANOBACTERIUM MEDIUM (DSMZ Medium 119)

[Ref.: #21024]

Culture medium growth

[Ref.: #21024]

Culture medium link

Medium recipe at MediaDive

[Ref.: #21024]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #21024]	growth	30 °C
[Ref.: #31177]	growth	05-37 °C
[Ref.: #31177]	optimum	20-30 °C

	pH	Kind of pH		pH
[Ref.: #31177]			optimum	7.0-7.5
[Ref.: #31177]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #21024]	Oxygen tolerance	anaerobe
[Ref.: #31177]	Oxygen tolerance	anaerobe
[Ref.: #125438]	Oxygen tolerance	anaerobe Confidence in %91.639

[Ref.: #31177]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31177]		NaCl		growth	0-0.3	%

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #31177]		acetate ChEBI	+	carbon source

	Metabolite production	Metabolite	Production
[Ref.: #31177]		methane ChEBI	yes

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #21024]	Sample type/isolated from	granulated biomass of an anaerobic expanded granular sludge bed bioreactor operated at low temperature
[Ref.: #21024]	Geographic location (country and/or sea, region)	Wageningen
[Ref.: #21024]	Country	Netherlands
[Ref.: #21024]	Country ISO 3 Code	NLD
[Ref.: #21024]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Bioreactor	-
#Engineered	#Waste	#Activated sludge
#Condition	#Anoxic (anaerobic)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HF569045 (>99% sequence identity) for Methanospirillum stamsii subclade from Microbeatlas

Aquatic Samples	450
Soil Samples	17
Animal Samples	25
Plant Samples	75
Total Samples	567

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #21024]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #21024]	Methanospirillum stamsii partial 16S rRNA gene, strain DSM 26304	HF569045	1408	GenBank ENA	1277351 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methanospirillum stamsii Pt1	GCA_003173335	contig	GenBank ENA	1277351 tax ID	*

[Ref.: #21024]	GC-content	40 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #31177]	GC-content	40 mol%

Data predicted using genome information as a basis Genome-based predictions

	Model	Trait	Prediction	Confidence in %	In training data
	Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanospirillum stamsii Pt1 NCBI: GCA_003173335.1
[Ref.: #125438]	gram-positive	gram-positiveⓘ	no	70.836	yes
[Ref.: #125438]	anaerobic	anaerobicⓘ	yes	91.639	yes
[Ref.: #125438]	aerobic	aerobicⓘ	no	92.917	no
[Ref.: #125438]	spore-forming	spore-formingⓘ	no	86.324	yes
[Ref.: #125438]	thermophile	thermophilicⓘ	no	84.398	yes
[Ref.: #125438]	motile2+	flagellatedⓘ	no	85.034	no

Availability in culture collections External links

[Ref.: #21024]

Culture collection no.

DSM 26304, VKM B-2808

[Ref.: #88126]

SI-ID 398829

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanospirillum stamsii sp. nov., a psychrotolerant, hydrogenotrophic, methanogenic archaeon isolated from an anaerobic expanded granular sludge bed bioreactor operated at low temperature.	Parshina SN, Ermakova AV, Bomberg M, Detkova EN	Int J Syst Evol Microbiol	10.1099/ijs.0.056218-0	2013	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#21024

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26304

#31177

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27504 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#88126

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID398829.1 )

#125438

Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )

#27504

IJSEM 180 2014 ( DOI 10.1099/ijs.0.056218-0 , PubMed 24048867 )

* These data were automatically processed and therefore are not curated

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Methanospirillum stamsii Pt1

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