Methanosarcina soligelidi (DSM 26065, JCM 18468, SMA-21)

Name: Methanosarcina soligelidi (DSM 26065, JCM 18468, SMA-21)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24170

Type strain

Strain Designation SMA-21

Culture col. no. DSM 26065 JCM 18468 SMA-21

NCBI tax ID(s) 1036677

Special Collections DSMZ JCM

Links

Methanosarcina soligelidi SMA-21 is an anaerobe, mesophilic prokaryote that was isolated from permafrost-affected soil .

anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 9425

LMG 15061,DSM 20065,NCFB 2477,NCIMB 702477,NCDO2477,CIP 107090,DSM 26065,JCM 18468

@ref 20215

Last LPSN update 2025-12-16 09:52:47

Domain Methanobacteriati

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanosarcinales

Family Methanosarcinaceae

Genus Methanosarcina

Species Methanosarcina soligelidi

Full scientific name Methanosarcina soligelidi Wagner et al. 2013

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	97.6

Colony morphology

21023

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21023	METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a)		Medium recipe at MediaDive	Name: METHANOSARCINA BARKERI MEDIUM (DSMZ Medium 120a) Composition: Methanol 15.4688 g/l NaCl 2.19727 g/l Yeast extract 1.95312 g/l Casitone 1.95312 g/l NaHCO3 0.830078 g/l NH4Cl 0.488281 g/l MgSO4 x 7 H2O 0.488281 g/l K2HPO4 0.341797 g/l Na2S x 9 H2O 0.292969 g/l L-Cysteine HCl x H2O 0.292969 g/l CaCl2 x 2 H2O 0.244141 g/l KH2PO4 0.224609 g/l HCl 0.00244141 g/l FeSO4 x 7 H2O 0.00195312 g/l FeCl2 x 4 H2O 0.00146484 g/l Sodium resazurin 0.000488281 g/l CoCl2 x 6 H2O 0.000185547 g/l Pyridoxine hydrochloride 9.76563e-05 g/l MnCl2 x 4 H2O 9.76563e-05 g/l ZnCl2 6.83594e-05 g/l Thiamine HCl 4.88281e-05 g/l Riboflavin 4.88281e-05 g/l Calcium D-(+)-pantothenate 4.88281e-05 g/l Nicotinic acid 4.88281e-05 g/l p-Aminobenzoic acid 4.88281e-05 g/l (DL)-alpha-Lipoic acid 4.88281e-05 g/l Na2MoO4 x 2 H2O 3.51562e-05 g/l NiCl2 x 6 H2O 2.34375e-05 g/l Folic acid 1.95312e-05 g/l Biotin 1.95312e-05 g/l H3BO3 5.85938e-06 g/l CuCl2 x 2 H2O 1.95313e-06 g/l Vitamin B12 9.76563e-07 g/l H2SO4 Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21023	positive	growth	28	mesophilic

Physiology and metabolism

Oxygen tolerance

21023

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125439		95.7

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Permafrost
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude
21023	permafrost-affected soil (Psammentic Aquorthel)	Psammentic Aquorthel	Siberia, Lena Delta, Samoylov Island (72° 22' N 126° 28' E),	Russia	RUS	Asia	72.3667	126.467 72.3667/126.467

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21023	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM74431v1 assembly for Methanosarcina soligelidi SMA-21	contig	GCA_000744315	1036677.3	2576861399	1036677	78.31

Genome browser: GCA_000744315

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21023	Methanosarcina soligelidi strain SMA-21 16S ribosomal RNA gene, partial sequence	JF812255	1337		1036677
124043	Methanosarcina soligelidi gene for 16S ribosomal RNA, partial sequence, strain: DSM 26065.	AB973359	1406		1036677

GC content

@ref	GC-content (mol%)	Method
21023	40.9	high performance liquid chromatography (HPLC)
21023	41.5	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanosarcina soligelidi SMA-21
NCBI: GCA_000744315

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	95.70	no
125439	motility	BacteriaNetⓘ	no	80.10	no
125439	gram_stain	BacteriaNetⓘ	negative	97.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	81.70	no

Literature

Title	Authors	Journal	DOI	Year
Method for Indirect Quantification of CH4 Production via H2O Production Using Hydrogenotrophic Methanogens.	Taubner RS, Rittmann SK.	Front Microbiol	10.3389/fmicb.2016.00532	2016
Methanosarcina Spherical Virus, a Novel Archaeal Lytic Virus Targeting Methanosarcina Strains.	Weidenbach K, Nickel L, Neve H, Alkhnbashi OS, Kunzel S, Kupczok A, Bauersachs T, Cassidy L, Tholey A, Backofen R, Schmitz RA.	J Virol	10.1128/jvi.00955-17	2017
Assessing the Ecophysiology of Methanogens in the Context of Recent Astrobiological and Planetological Studies.	Taubner RS, Schleper C, Firneis MG, Rittmann SK.	Life (Basel)	10.3390/life5041652	2015
Response of Methanogenic Archaea from Siberian Permafrost and Non-permafrost Environments to Simulated Mars-like Desiccation and the Presence of Perchlorate.	Serrano P, Alawi M, de Vera JP, Wagner D	Astrobiology	10.1089/ast.2018.1877	2019
Enhanced Radiation Resistance of Methanosarcina soligelidi SMA-21, a New Methanogenic Archaeon Isolated from a Siberian Permafrost-Affected Soil in Direct Comparison to Methanosarcina barkeri.	Morozova D, Moeller R, Rettberg P, Wagner D	Astrobiology	10.1089/ast.2015.1319	2015
Genome Sequence of Methanosarcina soligelidi SMA-21, Isolated from Siberian Permafrost-Affected Soil.	Alawi M, Shapiro N, Woyke T, Horn F, Bakermans C, Wagner D	Genome Announc	10.1128/genomeA.00270-15	2015
Methanosarcina soligelidi sp. nov., a desiccation- and freeze-thaw-resistant methanogenic archaeon from a Siberian permafrost-affected soil.	Wagner D, Schirmack J, Ganzert L, Morozova D, Mangelsdorf K	Int J Syst Evol Microbiol	10.1099/ijs.0.046565-0	2013

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21023	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26065
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanosarcina soligelidi (DSM 26065, JCM 18468, SMA-21)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000744315

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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