Name: Ligilactobacillus pobuzihii E100301
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 24127

Type strain:

Species: Ligilactobacillus pobuzihii

Strain Designation: E100301

Culture col. no.: DSM 28122, KCTC 13174, NBRC 103219, RIFY 6501, JCM 18084, CIP 110289

Strain history: CIP <- 2011, KCTC

NCBI tax ID(s): 449659 (species)

SI-ID 370396

KCTC 13174, NBRC 103219, JCM 18084, LMG 28475, CIP 110289

Section

Ligilactobacillus pobuzihii E100301 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from fermented cummingcordia .

Gram-positive
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Ligilactobacillus*
	Species	**Ligilactobacillus pobuzihii**
	Full Scientific Name (LPSN)	Ligilactobacillus pobuzihii (Chen et al. 2010) Zheng et al. 2020

Synonym

Lactobacillus pobuzihii

Information on morphological and physiological properties Morphology

[Ref.: #29607]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #121918]	Gram stain	positive

[Ref.: #29607]	Cell length	2.2-3.9 µm

[Ref.: #29607]	Cell width	0.5 µm

[Ref.: #29607]	Cell shape	rod-shaped
[Ref.: #121918]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #121918]	Motility	no

[Ref.: #29607]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20987]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #20987]

Culture medium growth

[Ref.: #20987]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20987]

Culture medium composition

expand / minimize

[Ref.: #37969]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #37969]

Culture medium growth

[Ref.: #37969]

Culture medium composition

expand / minimize

[Ref.: #121918]

Culture medium

CIP Medium 40

[Ref.: #121918]

Culture medium growth

[Ref.: #121918]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #20987]	growth	37 °C
[Ref.: #29607]	growth	30-37 °C
[Ref.: #29607]	optimum	37 °C
[Ref.: #37969]	growth	37 °C
[Ref.: #67770]	growth	37 °C
[Ref.: #67771]	growth	37 °C

[Ref.: #20987]	Temperature range	mesophilic
[Ref.: #29607]	Temperature range	mesophilic
[Ref.: #37969]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #29607]			optimum	7
[Ref.: #29607]			growth	7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #20987]	Oxygen tolerance	microaerophile
[Ref.: #29607]	Oxygen tolerance	facultative anaerobe
[Ref.: #67771]	Oxygen tolerance	facultative anaerobe
[Ref.: #121918]	Oxygen tolerance	facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #29607]		NaCl		growth	10	%
[Ref.: #29607]		NaCl		optimum	10	%

[Ref.: #29607]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #29607]	5-dehydro-D-gluconate ChEBI	+	carbon source
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29607]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #29607]	fructose ChEBI	+	carbon source
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29607]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29607]	lactose ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #29607]	maltose ChEBI	+	carbon source
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #121918]	nitrate ChEBI	-	reduction
[Ref.: #121918]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29607]	rhamnose ChEBI	+	carbon source
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29607]	ribose ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #29607]	trehalose ChEBI	+	carbon source
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #121918]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121918]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121918]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121918]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #20987]	14838	-	-	-	-	+	+	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	+	-	+	-	-	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121918]	12492	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20987]	Sample type/isolated from	fermented cummingcordia (pobuzihi)
[Ref.: #20987]	Country	Taiwan
[Ref.: #20987]	Country ISO 3 Code	TWN
[Ref.: #20987]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Fermented cummingcordia (pobuzihi)
[Ref.: #67770]	Country	Taiwan
[Ref.: #67770]	Country ISO 3 Code	TWN
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From pobuzih, fermented cummingcordia
[Ref.: #67771]	Geographic location (country and/or sea, region)	Taichung city
[Ref.: #67771]	Country	Taiwan
[Ref.: #67771]	Country ISO 3 Code	TWN
[Ref.: #67771]	Continent	Asia

[Ref.: #121918]	Sample type/isolated from	Food, Fermented cummingcordia, food
[Ref.: #121918]	Country	Taiwan
[Ref.: #121918]	Country ISO 3 Code	TWN
[Ref.: #121918]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Vegetable (incl. Grains)
#Engineered	#Food production	#Food
#Engineered	#Food production	#Fermented

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC521982 (>99% sequence identity) for Ligilactobacillus pobuzihii from Microbeatlas

Aquatic Samples	41
Soil Samples	81
Animal Samples	2303
Plant Samples	41
Total Samples	2466

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20987]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121918]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Lactobacillus pobuzihii JCM 18084 gene for 16S rRNA, partial sequence	LC521982	1500	ENA	449659 tax ID
[Ref.: #20987]	Lactobacillus pobuzihii gene for 16S rRNA, partial sequence, strain: NBRC 103219	AB326358	1489	ENA	1242253 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Ligilactobacillus pobuzihii NBRC 103219	GCA_001438865	scaffold	GenBank	449659 tax ID
[Ref.: #67771]	Ligilactobacillus pobuzihii E100301 = KCTC 13174	GCA_000349725	scaffold	GenBank	1242253 tax ID
[Ref.: #67771]	Ligilactobacillus pobuzihii E100301 = KCTC 13174	GCA_001433815	contig	GenBank	1242253 tax ID
[Ref.: #66792]	Lactobacillus pobuzihii strain NBRC 103219	449659.4	wgs	PATRIC	449659 tax ID	*
[Ref.: #66792]	Ligilactobacillus pobuzihii E100301	2554235039	draft	JGI IMG/M	1242253 tax ID	*
[Ref.: #66792]	Ligilactobacillus pobuzihii NBRC 103219	2667528017	draft	JGI IMG/M	449659 tax ID	*

[Ref.: #20987]	GC-content	37.2 mol%
[Ref.: #67770]	GC-content	37.7 mol%	genome sequence analysis
[Ref.: #67771]	GC-content	37.2 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus pobuzihii E100301 = KCTC 13174 PATRIC: 1242253.4
[Ref.: #69480]	motile	no	93.882	no
[Ref.: #69480]	gram-positive	yes	96.858	no
[Ref.: #69480]	anaerobic	no	90.807	yes
[Ref.: #69480]	aerobic	no	95.934	no
[Ref.: #69480]	halophile	yes	95.166	no
[Ref.: #69480]	spore-forming	no	95.639	no
[Ref.: #69480]	glucose-util	yes	91.656	yes
[Ref.: #69480]	flagellated	no	97.789	no
[Ref.: #69480]	thermophile	no	99.518	yes
[Ref.: #69480]	glucose-ferment	yes	90.299	no

Availability in culture collections External links

[Ref.: #20987]

Culture collection no.

DSM 28122, KCTC 13174, NBRC 103219, RIFY 6501, JCM 18084, CIP 110289

[Ref.: #88086]

SI-ID 370396

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Lactobacillus salitolerans sp. nov., a novel lactic acid bacterium isolated from spent mushroom substrates.	Tohno M, Tanizawa Y, Kojima Y, Sakamoto M, Nakamura Y, Ohkuma M, Kobayashi H	Int J Syst Evol Microbiol	10.1099/ijsem.0.003224	2019	*
Genetics	Draft Genome Sequence of Lactobacillus pobuzihii E100301T.	Chiu CM, Chang CH, Pan SF, Wu HC, Li SW, Chang CH, Lee YS, Chiang CM, Chen YS	Genome Announc	10.1128/genomeA.00185-13	2013	*
Phylogeny	Lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented cummingcordia).	Chen YS, Miyashita M, Suzuki KI, Sato H, Hsu JS, Yanagida F	Int J Syst Evol Microbiol	10.1099/ijs.0.016873-0	2009	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20987

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28122

#29607

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25997 (see below)

#37969 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#88086

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID370396.1 )

#121918 Collection of Institut Pasteur ; Curators of the CIP; CIP 110289

#25997

IJSEM 1914 2010 ( DOI 10.1099/ijs.0.016873-0 , PubMed 19783610 )

* These data were automatically processed and therefore are not curated

Change proposal

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Search for species Ligilactobacillus pobuzihii in external resources:
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Lactobacillus pobuzihii E100301 = KCTC 13174

Change proposal

Change proposal