Halohasta litchfieldiae (DSM 22187, CGMCC 1.12394, JCM 15066)

Name: Halohasta litchfieldiae (DSM 22187, CGMCC 1.12394, JCM 15066)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 24090

Type strain

Strain Designation t-ADL

Culture col. no. DSM 22187 CGMCC 1.12394 JCM 15066 t-ADL

NCBI tax ID(s) 1073996

Special Collections DSMZ JCM

History <- M. Dyall-Smith, Dept. Membrane Biochemistry, Max Planck Inst. Biochemistry, Martinsried, Germany; t-ADL <- D. Oh and M. Dyall-Smith D. Oh and M. L. Dyall-Smith tADL.

Links

Halohasta litchfieldiae t-ADL is a mesophilic prokaryote that was isolated from surface water.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 407294

JCM 15066,DSM 22187

@ref 20215

Last LPSN update 2026-02-09 11:23:53

Domain Archaea

Phylum Methanobacteriota

Class Halobacteria

Order Halobacteriales

Family Haloferacaceae

Genus Halohasta

Species Halohasta litchfieldiae

Full scientific name Halohasta litchfieldiae Mou et al. 2013

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
21256	HALOQUADRATUM WALSBYI MEDIUM (DSMZ Medium 1091)		Medium recipe at MediaDive	Name: HALOQUADRATUM WALSBYI MEDIUM (DSMZ Medium 1091) Composition: NaCl 195.0 g/l MgSO4 x 7 H2O 50.0 g/l MgCl2 x 6 H2O 35.0 g/l KCl 5.0 g/l Tris buffer 2.4228 g/l Sodium pyruvate 1.0 g/l NaNO3 1.0 g/l CaCl2 x 2 H2O 0.5 g/l NaHCO3 0.25 g/l KH2PO4 0.05 g/l Yeast extract 0.05 g/l NH4Cl 0.03 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
21256	positive	growth	28	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	enterobactin biosynthesis	100	3 of 3
66794	methylglyoxal degradation	100	5 of 5
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	factor 420 biosynthesis	100	5 of 5
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	ceramide biosynthesis	100	1 of 1
66794	denitrification	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	folate polyglutamylation	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	flavin biosynthesis	93.33	14 of 15
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	palmitate biosynthesis	86.36	19 of 22
66794	vitamin B1 metabolism	84.62	11 of 13
66794	NAD metabolism	83.33	15 of 18
66794	vitamin K metabolism	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	starch degradation	80	8 of 10
66794	Entner Doudoroff pathway	80	8 of 10
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	valine metabolism	77.78	7 of 9
66794	allantoin degradation	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	gluconeogenesis	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	glutamate and glutamine metabolism	75	21 of 28
66794	glycogen biosynthesis	75	3 of 4
66794	lipid metabolism	74.19	23 of 31
66794	non-pathway related	73.68	28 of 38
66794	vitamin B12 metabolism	73.53	25 of 34
66794	proline metabolism	72.73	8 of 11
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	photosynthesis	71.43	10 of 14
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	glycolysis	70.59	12 of 17
66794	purine metabolism	70.21	66 of 94
66794	threonine metabolism	70	7 of 10
66794	propionate fermentation	70	7 of 10
66794	pyrimidine metabolism	68.89	31 of 45
66794	acetoin degradation	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	pantothenate biosynthesis	66.67	4 of 6
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	cyanate degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	citric acid cycle	64.29	9 of 14
66794	glutathione metabolism	64.29	9 of 14
66794	degradation of pentoses	64.29	18 of 28
66794	heme metabolism	64.29	9 of 14
66794	carotenoid biosynthesis	63.64	14 of 22
66794	metabolism of disaccharids	63.64	7 of 11
66794	ketogluconate metabolism	62.5	5 of 8
66794	arginine metabolism	62.5	15 of 24
66794	alanine metabolism	62.07	18 of 29
66794	methionine metabolism	61.54	16 of 26
66794	leucine metabolism	61.54	8 of 13
66794	3-chlorocatechol degradation	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	lipoate biosynthesis	60	3 of 5
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	oxidative phosphorylation	59.34	54 of 91
66794	propanol degradation	57.14	4 of 7
66794	mevalonate metabolism	57.14	4 of 7
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	degradation of sugar alcohols	56.25	9 of 16
66794	cysteine metabolism	55.56	10 of 18
66794	nitrate assimilation	55.56	5 of 9
66794	tryptophan metabolism	55.26	21 of 38
66794	histidine metabolism	55.17	16 of 29
66794	pentose phosphate pathway	54.55	6 of 11
66794	lysine metabolism	52.38	22 of 42
66794	sulfopterin metabolism	50	2 of 4
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	CDP-diacylglycerol biosynthesis	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	tyrosine metabolism	50	7 of 14
66794	selenocysteine biosynthesis	50	3 of 6
66794	glycine metabolism	50	5 of 10
66794	biotin biosynthesis	50	2 of 4
66794	kanosamine biosynthesis II	50	1 of 2
66794	degradation of sugar acids	48	12 of 25
66794	sulfate reduction	46.15	6 of 13
66794	urea cycle	46.15	6 of 13
66794	degradation of hexoses	44.44	8 of 18
66794	lipid A biosynthesis	44.44	4 of 9
66794	arachidonic acid metabolism	44.44	8 of 18
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	metabolism of amino sugars and derivatives	40	2 of 5
66794	phenol degradation	40	8 of 20
66794	creatinine degradation	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	d-xylose degradation	36.36	4 of 11
66794	cholesterol biosynthesis	36.36	4 of 11
66794	ascorbate metabolism	36.36	8 of 22
66794	isoprenoid biosynthesis	34.62	9 of 26
66794	IAA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	methanogenesis from CO2	33.33	4 of 12
66794	phosphatidylethanolamine bioynthesis	30.77	4 of 13
66794	aclacinomycin biosynthesis	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	vitamin B6 metabolism	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	lactate fermentation	25	1 of 4
66794	butanoate fermentation	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	ppGpp biosynthesis	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	vitamin E metabolism	25	1 of 4
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	polyamine pathway	21.74	5 of 23

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Surface water

Isolation

@ref	Sample type	Geographic location	Continent	Latitude	Longitude	Country	Country ISO 3 Code
21256	surface water	Deep Lake (68° 33' 35.6'' S, 78° 11' 44.9'' E)	Australia and Oceania	-68.5599	78.1958 -68.5599/78.1958
67770	Deep Lake		Antarctica			Antarctica	ATA

Taxonmaps

69479

Global distribution of 16S sequence JF421969 (>99% sequence identity) for Halohasta from Microbeatlas

Aquatic Samples	120
Soil Samples	84
Animal Samples
Plant Samples
Total Samples	204

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
21256	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM278821v1 assembly for Halohasta litchfieldiae tADL	complete	GCA_002788215	758602.3	2506783051	1073996	98.04
67770	IMG-taxon 2693429898 annotated assembly for Halohasta litchfieldiae DSM 22187	contig	GCA_900109065	1073996.3	2693429898	1073996	62.43

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
21256	Halohasta litchfieldiae strain tADL 16S ribosomal RNA gene, partial sequence	JF421969	1473		1073996
124043	Halohasta litchfieldiae gene for 16S ribosomal RNA, partial sequence, strain: JCM 15066.	AB935408	1476		1073996

GC content

@ref	GC-content (mol%)	Method
21256	62.9	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Halohasta litchfieldiae DSM 22187
NCBI: GCA_900109065

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	89.10	no
125439	motility	BacteriaNetⓘ	no	52.90	no
125439	gram_stain	BacteriaNetⓘ	positive	53.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	63.30	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Characterization of an archaeal virus-host system reveals massive genomic rearrangements in a laboratory strain.	Mercier C, Thies D, Zhong L, Raftery MJ, Erdmann S.	Front Microbiol	10.3389/fmicb.2023.1274068	2023
Genetics	Isolation and Taxonomic Characterization of Novel Haloarchaeal Isolates From Indian Solar Saltern: A Brief Review on Distribution of Bacteriorhodopsins and V-Type ATPases in Haloarchaea.	Verma DK, Chaudhary C, Singh L, Sidhu C, Siddhardha B, Prasad SE, Thakur KG.	Front Microbiol	10.3389/fmicb.2020.554927	2020
Phylogeny	Halohasta litorea gen. nov. sp. nov., and Halohasta litchfieldiae sp. nov., isolated from the Daliang aquaculture farm, China and from Deep Lake, Antarctica, respectively.	Mou YZ, Qiu XX, Zhao ML, Cui HL, Oh D, Dyall-Smith ML	Extremophiles	10.1007/s00792-012-0485-5	2012

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#21256	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22187
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Halohasta litchfieldiae (DSM 22187, CGMCC 1.12394, JCM 15066)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002788215

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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