Name: Shouchella shacheensis HNA-14
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 23666

Type strain:

Species: Shouchella shacheensis

Strain Designation: HNA-14

Culture col. no.: DSM 26902, CICC 10559, KCTC 33145, SCULCB HNA-14

Strain history: <- Renyuan Ye, China

NCBI tax ID(s): 1649580 (species)

SI-ID 401371

DSM 26902

Section

Shouchella shacheensis HNA-14 is an aerobe, spore-forming, mesophilic bacterium that was isolated from saline-alkali soil.

motile
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Shouchella*
	Species	**Shouchella shacheensis**
	Full Scientific Name (LPSN)	Shouchella shacheensis (Lei et al. 2014) Joshi et al. 2022

	Synonym	Alkalihalobacillus shacheensis
	Synonym	Bacillus shacheensis

Information on morphological and physiological properties Morphology

[Ref.: #67771]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #67771]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %94.66

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20405]

Culture medium

CASO AGAR (MERCK 105458) (DSMZ Medium 220)

[Ref.: #20405]

Culture medium growth

[Ref.: #20405]

Culture medium link

Medium recipe at MediaDive

[Ref.: #20405]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #67771]			growth	30 °C

[Ref.: #67771]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

aerobe

[Ref.: #67771]	Type of spore	spore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %99
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.999

[Ref.: #67771]

Observation

quinones: MK-7

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #20405]	Sample type/isolated from	saline-alkali soil
[Ref.: #20405]	Geographic location (country and/or sea, region)	Xinjiang Province, Shache County
[Ref.: #20405]	Country	China
[Ref.: #20405]	Country ISO 3 Code	CHN
[Ref.: #20405]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From a saline-alkali soil sample collected in Shache County
[Ref.: #67771]	Geographic location (country and/or sea, region)	Xinjiang Province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Condition	#Alkaline	-
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ620634 (>99% sequence identity) for Alkalihalobacillus shacheensis subclade from Microbeatlas

Aquatic Samples	5
Soil Samples	9
Animal Samples	13
Plant Samples	7
Total Samples	34

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #20405]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20405]	Bacillus shacheensis strain HNA-14 16S ribosomal RNA gene, partial sequence	HQ620634	1570	ENA	1649580 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Shouchella shacheensis HNA-14	GCA_001484965	scaffold	GenBank	1649580 tax ID	*

[Ref.: #20405]	GC-content	46.8 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	48.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Alkalihalobacillus shacheensis HNA-14 NCBI: GCA_001484965.1
[Ref.: #69481]	spore-forming	yes	99	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Alkalihalobacillus shacheensis HNA-14 NCBI: GCA_001484965.1
[Ref.: #69480]	flagellated	yes	64.496	no
[Ref.: #69480]	gram-positive	yes	91.035	yes
[Ref.: #69480]	anaerobic	no	99.64	yes
[Ref.: #69480]	aerobic	yes	95.269	no
[Ref.: #69480]	halophile	yes	89.992	no
[Ref.: #69480]	spore-forming	yes	95.153	yes
[Ref.: #69480]	glucose-util	yes	91.076	no
[Ref.: #69480]	motile	yes	87.777	yes
[Ref.: #69480]	thermophile	no	99.242	yes
[Ref.: #69480]	glucose-ferment	no	87.009	no

Availability in culture collections External links

[Ref.: #20405]

Culture collection no.

DSM 26902, CICC 10559, KCTC 33145, SCULCB HNA-14

[Ref.: #87726]

SI-ID 401371

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.	Lei Z, Qiu P, Ye R, Tian J, Liu Y, Wang L, Tang SK, Li WJ, Tian Y	J Gen Appl Microbiol	DN/JST.JSTAGE/jgam/60.101	2014	*
	Adaptations of Bacillus shacheensis HNA-14 required for long-term survival under osmotic challenge: a multi-omics perspective.	Long X, Tian J, Liao X, Tian Y	RSC Adv	10.1039/c8ra05472j	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20405

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 26902

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87726

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID401371.1 )

* These data were automatically processed and therefore are not curated

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Alkalihalobacillus shacheensis HNA-14

Alkalihalobacillus shacheensis HNA-14

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