Strain identifier
BacDive ID: 23666
Type strain: 
Species: Shouchella shacheensis
Strain Designation: HNA-14
Strain history: <- Renyuan Ye, China
NCBI tax ID(s): 1649580 (species)
version 8.1 (current version)
General
@ref: 20405
BacDive-ID: 23666
DSM-Number: 26902
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, motile, rod-shaped
description: Shouchella shacheensis HNA-14 is an aerobe, spore-forming, mesophilic bacterium that was isolated from saline-alkali soil.
NCBI tax id
- NCBI tax id: 1649580
- Matching level: species
strain history
| @ref | history |
|---|---|
| 20405 | <- R. Ye, Sichuan Univ., College of Light Ind., Textile and Food Eng., Chengdu, China; HNA-14 <- L. Yang |
| 67771 | <- Renyuan Ye, China |
doi: 10.13145/bacdive23666.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Shouchella
- species: Shouchella shacheensis
- full scientific name: Shouchella shacheensis (Lei et al. 2014) Joshi et al. 2022
synonyms
@ref synonym 20215 Alkalihalobacillus shacheensis 20215 Bacillus shacheensis
@ref: 20405
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Caryophanales
family: Bacillaceae
genus: Shouchella
species: Shouchella shacheensis
full scientific name: Shouchella shacheensis (Lei et al. 2014) Joshi et al. 2022
strain designation: HNA-14
type strain: yes
Morphology
cell morphology
| @ref | cell shape | motility | gram stain | confidence |
|---|---|---|---|---|
| 67771 | rod-shaped | yes | ||
| 67771 | positive | |||
| 69480 | yes | 94.66 | ||
| 69480 | positive | 100 |
Culture and growth conditions
culture medium
- @ref: 20405
- name: CASO AGAR (MERCK 105458) (DSMZ Medium 220)
- growth: yes
- link: https://mediadive.dsmz.de/medium/220
- composition: Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 15.0 g/l Casein peptone 15.0 g/l Soy peptone 5.0 g/l Distilled water
culture temp
- @ref: 67771
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
- @ref: 67771
- oxygen tolerance: aerobe
spore formation
| @ref | type of spore | spore formation | confidence |
|---|---|---|---|
| 67771 | spore | yes | |
| 69481 | yes | 99 | |
| 69480 | yes | 99.999 |
observation
- @ref: 67771
- observation: quinones: MK-7
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|
| 20405 | saline-alkali soil | Xinjiang Province, Shache County | China | CHN | Asia |
| 67771 | From a saline-alkali soil sample collected in Shache County | Xinjiang Province | China | CHN | Asia |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Alkaline | |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_1181.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_499;97_803;98_931;99_1181&stattab=map
- Last taxonomy: Alkalihalobacillus shacheensis subclade
- 16S sequence: HQ620634
- Sequence Identity:
- Total samples: 34
- soil counts: 9
- aquatic counts: 5
- animal counts: 13
- plant counts: 7
Safety information
risk assessment
- @ref: 20405
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20405
- description: Bacillus shacheensis strain HNA-14 16S ribosomal RNA gene, partial sequence
- accession: HQ620634
- length: 1570
- database: ena
- NCBI tax ID: 1649580
Genome sequences
- @ref: 66792
- description: Shouchella shacheensis HNA-14
- accession: GCA_001484965
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 1649580
GC content
| @ref | GC-content | method |
|---|---|---|
| 20405 | 46.8 | high performance liquid chromatography (HPLC) |
| 67771 | 48.6 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 99 | no |
| flagellated | yes | 64.496 | no |
| gram-positive | yes | 91.035 | yes |
| anaerobic | no | 99.64 | yes |
| aerobic | yes | 95.269 | no |
| halophile | yes | 89.992 | no |
| spore-forming | yes | 95.153 | yes |
| glucose-util | yes | 91.076 | no |
| motile | yes | 87.777 | yes |
| thermophile | no | 99.242 | yes |
| glucose-ferment | no | 87.009 | no |
External links
@ref: 20405
culture collection no.: DSM 26902, CICC 10559, KCTC 33145, SCULCB HNA-14
straininfo link
- @ref: 87726
- straininfo: 401371
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 25008165 | Bacillus shacheensis sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil. | Lei Z, Qiu P, Ye R, Tian J, Liu Y, Wang L, Tang SK, Li WJ, Tian Y | J Gen Appl Microbiol | DN/JST.JSTAGE/jgam/60.101 | 2014 | Bacillus/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, Cluster Analysis, Cytosol/chemistry, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Hydrogen-Ion Concentration, Molecular Sequence Data, Peptidoglycan/analysis, Phospholipids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, Soil Microbiology, Temperature, Vitamin K 2/analysis | Genetics |
| 35540019 | Adaptations of Bacillus shacheensis HNA-14 required for long-term survival under osmotic challenge: a multi-omics perspective. | Long X, Tian J, Liao X, Tian Y | RSC Adv | 10.1039/c8ra05472j | 2018 |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 20405 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26902 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26902) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 67771 | Curators of the KCTC | https://kctc.kribb.re.kr/En/Kctc | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 | |
| 87726 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID401371.1 |