Name: Streptomyces viridis BK199
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 23306

Type strain:

Species: Streptomyces viridis

Strain Designation: BK199

Culture col. no.: DSM 42078, CGMCC 4.6824, KACC 21003

Strain history: <- S.-W. Kwon, KACC; KACC 21003

NCBI tax ID(s): 887475 (species)

SI-ID 402429

DSM 42078

Special Collections

Section

Streptomyces viridis BK199 is an aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from hay meadow soil.

Gram-positive
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kitasatosporales*
	Family	*Streptomycetaceae*
	Genus	*Streptomyces*
	Species	**Streptomyces viridis**
	Full Scientific Name (LPSN)	Streptomyces viridis Kim et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30851]	Gram stain	positive

[Ref.: #30851]	Cell length	0.7 µm

[Ref.: #30851]	Cell width	0.65 µm

[Ref.: #30851]	Cell shape	rod-shaped

[Ref.: #69340]	Colony color	Ivory (1014), brown beige (1001)
[Ref.: #69340]	Cultivation medium used	ISP 5

[Ref.: #69340]	Colony color	Ivory (1014), lemon yellow (1012), honey yellow (1005)
[Ref.: #69340]	Cultivation medium used	ISP 2

[Ref.: #69340]	Colony color	Light ivory (1014), light ivory (1015), sand yellow (1002)
[Ref.: #69340]	Cultivation medium used	ISP 3

[Ref.: #69340]	Colony color	Sand yellow (1002)
[Ref.: #69340]	Cultivation medium used	ISP 4

[Ref.: #69340]	Colony color	Sand yellow (1002), brown beige (1011), green brown (8000)
[Ref.: #69340]	Cultivation medium used	ISP 7

[Ref.: #69340]	Colony color	Sand yellow (1002), brown beige (1011), ivory (1014)
[Ref.: #69340]	Cultivation medium used	suter with tyrosine

[Ref.: #69340]	Colony color	Sand yellow (1002), brown beige (1011), ivory (1014)
[Ref.: #69340]	Cultivation medium used	suter without tyrosine

[Ref.: #69340]	Colony color	Sand yellow (1002), golden yellow (1004)
[Ref.: #69340]	Cultivation medium used	ISP 6

[Ref.: #30851]	Pigment production	yes
[Ref.: #69340]	Pigment production	no
[Ref.: #69340]	Pigment name	Melanin
[Ref.: #69340]	Pigment production	no
[Ref.: #69340]	Pigment name	soluble pigment

[Ref.: #69340]	Multicellular complex forming ability	yes
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Medium Name (multicellularity)	ISP 2

[Ref.: #69340]	Multicellular complex forming ability	yes
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Multicellular complex color	Cement grey (7033)
[Ref.: #69340]	Medium Name (multicellularity)	ISP 3

[Ref.: #69340]	Multicellular complex forming ability	yes
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Multicellular complex color	Signal white (9003)
[Ref.: #69340]	Medium Name (multicellularity)	ISP 4

[Ref.: #69340]	Multicellular complex forming ability	yes
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Multicellular complex color	Signal white (9003)
[Ref.: #69340]	Medium Name (multicellularity)	ISP 5

[Ref.: #69340]	Multicellular complex forming ability	no
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Medium Name (multicellularity)	ISP 6

[Ref.: #69340]	Multicellular complex forming ability	yes
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Multicellular complex color	Signal white (9003), cement grey (7033)
[Ref.: #69340]	Medium Name (multicellularity)	ISP 7

[Ref.: #69340]	Multicellular complex forming ability	yes
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Multicellular complex color	Signal white (9003)
[Ref.: #69340]	Medium Name (multicellularity)	suter with tyrosine

[Ref.: #69340]	Multicellular complex forming ability	yes
[Ref.: #69340]	Multicellular complex name	Aerial mycelium
[Ref.: #69340]	Multicellular complex color	Signal white (9003)
[Ref.: #69340]	Medium Name (multicellularity)	suter without tyrosine

Multimedia content

[Ref.: #18933]

Caption:

Medium 65 28°C

[Ref.: #18933]

Intellectual property rights:

[Ref.: #69340]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69340]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

[Ref.: #69340]

Caption:

Plates (65, ISP2, ISP3, ISP4, ISP5, ISP7)

[Ref.: #69340]

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18933]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #18933]

Culture medium growth

[Ref.: #18933]

Culture medium link

Medium recipe at MediaDive

[Ref.: #18933]

Culture medium composition

expand / minimize

[Ref.: #18933]

Culture medium

STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)

[Ref.: #18933]

Culture medium growth

[Ref.: #18933]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #18933]

Culture medium

ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)

[Ref.: #18933]

Culture medium growth

[Ref.: #18933]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #18933]	growth	28 °C
[Ref.: #30851]	growth	10-37 °C
[Ref.: #30851]	optimum	30 °C

	pH	Kind of pH		pH
[Ref.: #30851]			optimum	7
[Ref.: #30851]			growth	05-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30851]

Oxygen tolerance

aerobe

[Ref.: #30851]

Ability of spore formation

yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #69340]		NaCl		growth	0-10	%

[Ref.: #30851]

Observation

aggregates in chains

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #69340]	arabinose ChEBI	+	growth
[Ref.: #69340]	cellulose ChEBI	-	growth
[Ref.: #30851]	citrate ChEBI	+	carbon source
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #30851]	esculin ChEBI	+	hydrolysis
[Ref.: #69340]	fructose ChEBI	+	growth
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #69340]	glucose ChEBI	+	growth
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69340]	inositol ChEBI	+	growth
[Ref.: #30851]	L-arabitol ChEBI	+	carbon source
[Ref.: #30851]	lactate ChEBI	+	carbon source
[Ref.: #30851]	maltose ChEBI	+	carbon source
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69340]	mannose ChEBI	+	growth
[Ref.: #30851]	melibiose ChEBI	+	carbon source
[Ref.: #68379]	nitrate ChEBI	-	reduction	* from API Coryne
[Ref.: #69340]	raffinose ChEBI	-	growth
[Ref.: #69340]	rhamnose ChEBI	+	growth
[Ref.: #30851]	sorbitol ChEBI	+	carbon source
[Ref.: #30851]	succinate ChEBI	+	carbon source
[Ref.: #69340]	sucrose ChEBI	+	growth
[Ref.: #68379]	sucrose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #69340]	xylose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #30851]	catalase	+	1.11.1.6
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #30851]	gelatinase	+
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	-	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69340]	3833	-	-	-	+	-	+/-	+	-	+/-	-	+	not determinedn.d.	+	+/-	+/-	-	-	+/-	+	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69340]	6017	not determinedn.d.	+	+	+	+/-	+	+	+/-	-	-	+	+	-	+/-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #18933]	Sample type/isolated from	hay meadow soil
[Ref.: #18933]	Geographic location (country and/or sea, region)	Northumberland, Cockle Park Experimental Farm, Palace Leas hay meadow plot 6
[Ref.: #18933]	Country	United Kingdom
[Ref.: #18933]	Country ISO 3 Code	GBR
[Ref.: #18933]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Meadow
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence FR692117 (>99% sequence identity) for Streptomyces from Microbeatlas

Aquatic Samples	3
Soil Samples	94
Animal Samples	8
Plant Samples	40
Total Samples	145

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18933]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #18933]	Streptomyces viridis Kim et al. 2013 partial 16S rRNA gene, type strain BK199T	FR692117	1458	GenBank ENA	887475 tax ID

[Ref.: #18933]	GC-content	70.2 mol%	fluorimetric
[Ref.: #30851]	GC-content	70.2 mol%

Availability in culture collections External links

[Ref.: #18933]

Culture collection no.

DSM 42078, CGMCC 4.6824, KACC 21003

[Ref.: #87524]

SI-ID 402429

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces chlorus sp. nov. and Streptomyces viridis sp. nov., isolated from soil.	Kim BY, Rong X, Zucchi TD, Huang Y, Goodfellow M	Int J Syst Evol Microbiol	10.1099/ijs.0.045906-0	2012	*

References

#18933

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 42078

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30851

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27181 (see below)

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69340

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#87524

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID402429.1 )

#27181

IJSEM 1728 2013 ( DOI 10.1099/ijs.0.045906-0 , PubMed 22922536 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Streptomyces viridis in external resources:
SILVA
BRENDA
PANGAEA
GBIF