Strain identifier

BacDive ID: 23306

Type strain: Yes

Species: Streptomyces viridis

Strain Designation: BK199

Strain history: <- S.-W. Kwon, KACC; KACC 21003

NCBI tax ID(s): 887475 (species)

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General

@ref: 18933

BacDive-ID: 23306

DSM-Number: 42078

keywords: 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, rod-shaped

description: Streptomyces viridis BK199 is an aerobe, spore-forming, Gram-positive bacterium that builds an aerial mycelium and was isolated from hay meadow soil.

NCBI tax id

  • NCBI tax id: 887475
  • Matching level: species

strain history

  • @ref: 18933
  • history: <- S.-W. Kwon, KACC; KACC 21003

doi: 10.13145/bacdive23306.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Kitasatosporales
  • family: Streptomycetaceae
  • genus: Streptomyces
  • species: Streptomyces viridis
  • full scientific name: Streptomyces viridis Kim et al. 2013

@ref: 18933

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Streptomycetaceae

genus: Streptomyces

species: Streptomyces viridis

full scientific name: Streptomyces viridis Kim et al. 2013

strain designation: BK199

type strain: yes

Morphology

cell morphology

  • @ref: 30851
  • gram stain: positive
  • cell length: 0.7 µm
  • cell width: 0.65 µm
  • cell shape: rod-shaped

colony morphology

@refcolony colormedium used
69340Ivory (1014), brown beige (1001)ISP 5
69340Ivory (1014), lemon yellow (1012), honey yellow (1005)ISP 2
69340Light ivory (1014), light ivory (1015), sand yellow (1002)ISP 3
69340Sand yellow (1002)ISP 4
69340Sand yellow (1002), brown beige (1011), green brown (8000)ISP 7
69340Sand yellow (1002), brown beige (1011), ivory (1014)suter with tyrosine
69340Sand yellow (1002), brown beige (1011), ivory (1014)suter without tyrosine
69340Sand yellow (1002), golden yellow (1004)ISP 6

multicellular morphology

@refforms multicellular complexcomplex namemedium namecomplex color
69340yesAerial myceliumISP 2
69340yesAerial myceliumISP 3Cement grey (7033)
69340yesAerial myceliumISP 4Signal white (9003)
69340yesAerial myceliumISP 5Signal white (9003)
69340noAerial myceliumISP 6
69340yesAerial myceliumISP 7Signal white (9003), cement grey (7033)
69340yesAerial myceliumsuter with tyrosineSignal white (9003)
69340yesAerial myceliumsuter without tyrosineSignal white (9003)

pigmentation

@refproductionname
30851yes
69340noMelanin
69340nosoluble pigment

multimedia

@refmultimedia contentcaptionintellectual property rights
18933https://www.dsmz.de/microorganisms/photos/DSM_42078.jpgMedium 65 28°C© Leibniz-Institut DSMZ
69340DSM_42078_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69340DSM_42078_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18933GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
18933STARCH - MINERAL SALT - AGAR (STMS) (DSMZ Medium 252)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium252.pdf
18933ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium84.pdf

culture temp

@refgrowthtypetemperature
18933positivegrowth28
30851positivegrowth10-37
30851positiveoptimum30

culture pH

@refabilitytypepHPH range
30851positivegrowth05-09alkaliphile
30851positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 30851
  • oxygen tolerance: aerobe

spore formation

  • @ref: 30851
  • spore formation: yes

halophily

  • @ref: 69340
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 30851
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3085118403L-arabitol+carbon source
3085116947citrate+carbon source
3085124996lactate+carbon source
3085117306maltose+carbon source
3085128053melibiose+carbon source
3085130911sorbitol+carbon source
3085130031succinate+carbon source
308514853esculin+hydrolysis
6934022599arabinose+growth
6934062968cellulose-growth
6934028757fructose+growth
6934017234glucose+growth
6934017268inositol+growth
6934037684mannose+growth
6934016634raffinose-growth
6934026546rhamnose+growth
6934017992sucrose+growth
6934018222xylose+growth
6837917632nitrate-reduction
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose+fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
30851catalase+1.11.1.6
30851gelatinase+
68379gelatinase+
68379urease-3.5.1.5
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69340---+-+/-+-+/--+++/-+/---+/-+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
69340++++/-+++/---++-+/--+----

Isolation, sampling and environmental information

isolation

  • @ref: 18933
  • sample type: hay meadow soil
  • geographic location: Northumberland, Cockle Park Experimental Farm, Palace Leas hay meadow plot 6
  • country: United Kingdom
  • origin.country: GBR
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Meadow
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_3632.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15;96_102;97_1938;98_2384;99_3632&stattab=map
  • Last taxonomy: Streptomyces
  • 16S sequence: FR692117
  • Sequence Identity:
  • Total samples: 145
  • soil counts: 94
  • aquatic counts: 3
  • animal counts: 8
  • plant counts: 40

Safety information

risk assessment

  • @ref: 18933
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 18933
  • description: Streptomyces viridis Kim et al. 2013 partial 16S rRNA gene, type strain BK199T
  • accession: FR692117
  • length: 1458
  • database: nuccore
  • NCBI tax ID: 887475

GC content

@refGC-contentmethod
1893370.2fluorimetric
3085170.2

External links

@ref: 18933

culture collection no.: DSM 42078, CGMCC 4.6824, KACC 21003

straininfo link

  • @ref: 87524
  • straininfo: 402429

literature

  • topic: Phylogeny
  • Pubmed-ID: 22922536
  • title: Streptomyces chlorus sp. nov. and Streptomyces viridis sp. nov., isolated from soil.
  • authors: Kim BY, Rong X, Zucchi TD, Huang Y, Goodfellow M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.045906-0
  • year: 2012
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Streptomyces/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/analysis
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18933Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 42078)https://www.dsmz.de/collection/catalogue/details/culture/DSM-42078
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30851Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2718128776041
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69340Wink, J.https://cdn.dsmz.de/wink/DSM%2042078.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87524Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402429.1StrainInfo: A central database for resolving microbial strain identifiers