Name: Methylotenera versatilis 301
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 23100

Type strain:

Species: Methylotenera versatilis

Strain Designation: 301

Culture col. no.: DSM 24635, JCM 17579, VKM B-2679

Strain history: A. Vorobev 301 <-- M. Kalyuzhnaya.

NCBI tax ID(s): 1055487 (species)

SI-ID 400810

DSM 24635

Special Collections

Section

Methylotenera versatilis 301 is a facultative anaerobe, Gram-negative, motile bacterium that was isolated from freshwater sediment.

Gram-negative
motile
ovoid-shaped
facultative anaerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Nitrosomonadales*
	Family	*Methylophilaceae*
	Genus	*Methylotenera*
	Species	**Methylotenera versatilis**
	Full Scientific Name (LPSN)	Methylotenera versatilis Kalyuzhnaya et al. 2012

Information on morphological and physiological properties Morphology

[Ref.: #30081]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98

[Ref.: #30081]	Cell length	0.8-1.2 µm

[Ref.: #30081]	Cell width	0.3-0.7 µm

[Ref.: #30081]	Cell shape	ovoid-shaped

[Ref.: #30081]	Motility	yes

[Ref.: #19131]

Incubation period

1-2 days

[Ref.: #30081]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #19131]

Culture medium

HYPHOMICROBIUM MEDIUM (DSMZ Medium 1355)

[Ref.: #19131]

Culture medium growth

[Ref.: #19131]

Culture medium link

Medium recipe at MediaDive

[Ref.: #19131]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #19131]	growth	25 °C
[Ref.: #30081]	optimum	18-21 °C
[Ref.: #67770]	growth	25 °C

	pH	Kind of pH		pH
[Ref.: #30081]			optimum	5.8-6.5
[Ref.: #30081]			growth	4.2-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30081]

Oxygen tolerance

facultative anaerobe

[Ref.: #30081]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %92.23

[Ref.: #30081]

Observation

aggregates in clumps

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30081]	ethanol ChEBI	+	carbon source
[Ref.: #30081]	fructose ChEBI	+	carbon source
[Ref.: #30081]	nitrate ChEBI	+	reduction
[Ref.: #30081]	pyruvate ChEBI	+	carbon source

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #30081]		urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19131]	Sample type/isolated from	freshwater sediment
[Ref.: #19131]	Geographic location (country and/or sea, region)	Lake Washington
[Ref.: #19131]	Country	USA
[Ref.: #19131]	Country ISO 3 Code	USA
[Ref.: #19131]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Sediment of Lake Washington
[Ref.: #67770]	Geographic location (country and/or sea, region)	Seattle, WA
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence NR_074693 (>99% sequence identity) for Methylotenera versatilis subclade from Microbeatlas

Aquatic Samples	1731
Soil Samples	549
Animal Samples	80
Plant Samples	199
Total Samples	2559

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19131]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Methylotenera versatilis strain 301 16S ribosomal RNA, partial sequence	NR_074693	1526	GenBank ENA	1055487 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Methylotenera versatilis 301	GCA_000093025	complete	GenBank ENA	666681 tax ID	*
[Ref.: #66792]	Methylotenera versatilis 301	666681.3	complete	PATRIC	666681 tax ID	*
[Ref.: #66792]	Methylotenera versatilis 301, JCM 17579	646564551	complete	JGI IMG/M	666681 tax ID	*

[Ref.: #19131]	GC-content	42.6 mol%	sequence analysis
[Ref.: #30081]	GC-content	42.6 mol%
[Ref.: #67770]	GC-content	42.6 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Methylotenera versatilis 301 301 NCBI: GCA_000093025.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.426	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	70.389	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	92.23	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.395	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	68.29	no

Availability in culture collections External links

[Ref.: #19131]

Culture collection no.

DSM 24635, JCM 17579, VKM B-2679

[Ref.: #87338]

SI-ID 400810

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Novel methylotrophic isolates from lake sediment, description of Methylotenera versatilis sp. nov. and emended description of the genus Methylotenera.	Kalyuzhnaya MG, Beck DAC, Vorobev A, Smalley N, Kunkel DD, Lidstrom ME, Chistoserdova L	Int J Syst Evol Microbiol	10.1099/ijs.0.029165-0	2011	*

References

#19131

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24635

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30081

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26438 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#87338

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400810.1 )

#26438

IJSEM 106 2012 ( DOI 10.1099/ijs.0.029165-0 , PubMed 21335496 )

* These data were automatically processed and therefore are not curated

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Methylotenera versatilis 301 301

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