Name: Brevibacterium siliguriense MB18
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 22937

Type strain:

Species: Brevibacterium siliguriense

Strain Designation: MB18

Culture col. no.: DSM 23676, LMG 25772, JCM 30994

Strain history: LMG 25772 <-- R. Chakraborty and A. Kumar; Dept. of Biotechnol., Univ. of North Bengal, India; MB18.

NCBI tax ID(s): 1136497 (species)

SI-ID 369732

LMG 25772, DSM 23676, JCM 30994

Section

Brevibacterium siliguriense MB18 is an aerobe, Gram-positive, rod-shaped bacterium that was isolated from River water.

Gram-positive
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Brevibacteriaceae*
	Genus	*Brevibacterium*
	Species	**Brevibacterium siliguriense**
	Full Scientific Name (LPSN)	Brevibacterium siliguriense Kumar et al. 2013

Information on morphological and physiological properties Morphology

[Ref.: #30536]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #30536]	Cell length	2 µm

[Ref.: #30536]	Cell width	0.4 µm

[Ref.: #30536]	Cell shape	rod-shaped

[Ref.: #30536]	Motility	no

Information on culture and growth conditions Culture and growth conditions

		Temperature
[Ref.: #30536]	growth	20-37 °C
[Ref.: #30536]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #30536]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #30536]			optimum	7
[Ref.: #30536]			growth	05-12

Information on physiology and metabolism Physiology and metabolism

[Ref.: #30536]

Oxygen tolerance

aerobe

[Ref.: #30536]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.979

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #30536]		NaCl		growth	0-15	%
[Ref.: #30536]		NaCl		optimum	2	%

[Ref.: #67770]

Observation

quinones: MK-8(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #30536]	alanine ChEBI	+	carbon source
[Ref.: #30536]	arginine ChEBI	+	carbon source
[Ref.: #30536]	leucine ChEBI	+	carbon source
[Ref.: #30536]	nitrate ChEBI	+	reduction
[Ref.: #30536]	proline ChEBI	+	carbon source

	Enzyme	Enzyme activity	EC number
[Ref.: #30536]	alpha-galactosidase	+	3.2.1.22
[Ref.: #30536]	gelatinase	+
[Ref.: #30536]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #19217]	Sample type/isolated from	River water
[Ref.: #19217]	Geographic location (country and/or sea, region)	West Bengal, River Mahananda at Siliguri (26°44'23.20"N, 88°25'22.89"E)
[Ref.: #19217]	Country	India
[Ref.: #19217]	Country ISO 3 Code	IND
[Ref.: #19217]	Continent	Asia
[Ref.: #19217]	Geographic location	26.7398°/88.4230°

[Ref.: #67770]	Sample type/isolated from	Water sample from Mahananda River
[Ref.: #67770]	Geographic location (country and/or sea, region)	Siliguri, Darjeeling District, West Bengal
[Ref.: #67770]	Country	India
[Ref.: #67770]	Country ISO 3 Code	IND
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#River (Creek)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19217]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Brevibacterium siliguriense gene for 16S ribosomal RNA, partial sequence, strain: JCM 30994	LC082101	1464	ENA	1136497 tax ID
[Ref.: #19217]	Brevibacterium siliguriense partial 16S rRNA gene, type strain MB18T	AM937247	1433	ENA	1136497 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Brevibacterium siliguriense DSM 23676	GCA_900105315	chromosome	GenBank	1136497 tax ID	*
[Ref.: #66792]	Brevibacterium siliguriense strain DSM 23676	1136497.4	complete	PATRIC	1136497 tax ID	*
[Ref.: #66792]	Brevibacterium siliguriense DSM 23676	2634166531	draft	JGI IMG/M	1136497 tax ID	*

[Ref.: #19217]	GC-content	64.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #30536]	GC-content	64.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Brevibacterium siliguriense DSM 23676 NCBI: GCA_900105315.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Brevibacterium siliguriense strain DSM 23676 PATRIC: 1136497.4
[Ref.: #69480]	motile	no	93.271	no
[Ref.: #69480]	flagellated	no	97.325	yes
[Ref.: #69480]	gram-positive	yes	89.546	yes
[Ref.: #69480]	anaerobic	no	99.524	yes
[Ref.: #69480]	aerobic	yes	95.974	no
[Ref.: #69480]	halophile	yes	75.912	no
[Ref.: #69480]	spore-forming	no	91.992	no
[Ref.: #69480]	glucose-util	yes	89.513	no
[Ref.: #69480]	thermophile	no	98.639	yes
[Ref.: #69480]	glucose-ferment	no	91.996	no

Availability in culture collections External links

[Ref.: #19217]

Culture collection no.

DSM 23676, LMG 25772, JCM 30994

[Ref.: #87185]

SI-ID 369732

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brevibacterium siliguriense sp. nov., a facultatively oligotrophic bacterium isolated from river water.	Kumar A, Ince IA, Kati A, Chakraborty R	Int J Syst Evol Microbiol	10.1099/ijs.0.038281-0	2012	*

References

#19217

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23676

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#30536

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26867 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87185

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID369732.1 )

#26867

IJSEM 511 2013 ( DOI 10.1099/ijs.0.038281-0 , PubMed 22523163 )

* These data were automatically processed and therefore are not curated

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Brevibacterium siliguriense DSM 23676

Brevibacterium siliguriense strain DSM 23676

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