Name: Campylobacter gracilis DSM 19528
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 2133

Type strain:

Species: Campylobacter gracilis

Culture col. no.: DSM 19528, ATCC 33236, JCM 8538, CCUG 27720, NCTC 12738, FDC 1084, KCTC 15224

Strain history: ATCC 33236 <-- A. C. R. Tanner FDC 1084.

NCBI tax ID(s): 824 (species)

Other strains from Campylobacter gracilis

C. gracilis CCUG 12632
C. gracilis CCUG 13143
C. gracilis CCUG 15803
C. gracilis CCUG 22762, LMG 7616
C. gracilis CCUG 27721
C. gracilis CCUG 42204 B
C. gracilis JCM 10958
C. gracilis JCM 10959

Section

Campylobacter gracilis DSM 19528 is an anaerobe, mesophilic bacterium that was isolated from human gingival sulcus.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Epsilonproteobacteria*
	Order	*Campylobacterales*
	Family	*Campylobacteraceae*
	Genus	*Campylobacter*
	Species	**Campylobacter gracilis**
	Full Scientific Name (LPSN)	Campylobacter gracilis (Tanner et al. 1981) Vandamme et al. 1995

Synonym

Bacteroides gracilis

Information on morphological and physiological properties Morphology

[Ref.: #69480]

Gram stain

negative Confidence in %99.992

[Ref.: #8151]

Incubation period

3-7 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8151]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #8151]

Culture medium growth

[Ref.: #8151]

Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

[Ref.: #8151]

Culture medium

CHOPPED MEAT MEDIUM (DSMZ Medium 78)

[Ref.: #8151]

Culture medium growth

[Ref.: #8151]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8151]

Culture medium composition

expand / minimize

[Ref.: #8151]

Culture medium

BTU MEDIUM (DSMZ Medium 413)

[Ref.: #8151]

Culture medium growth

[Ref.: #8151]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8151]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #8151]	growth	37 °C
[Ref.: #48907]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #8151]	Temperature range	mesophilic
[Ref.: #48907]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #8151]	Oxygen tolerance	anaerobe
[Ref.: #48907]	Oxygen tolerance	anaerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.999

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68373]	acetate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	citrate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	D-glucose ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	hippurate ChEBI	-	hydrolysis	* from API CAM
[Ref.: #68373]	propionate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	succinate ChEBI	-	assimilation	* from API CAM
[Ref.: #68373]	urea ChEBI	-	hydrolysis	* from API CAM

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68373]	cefazolin ChEBI	yes	224	µg	* from API CAM
[Ref.: #68373]	erythromycin ChEBI	yes	14	µg	* from API CAM
[Ref.: #68373]	nalidixic acid ChEBI	yes	84	µg	* from API CAM

	Enzyme	Enzyme activity	EC number
[Ref.: #68373]	alkaline phosphatase	-	3.1.3.1	* from API CAM
[Ref.: #68373]	catalase	+	1.11.1.6	* from API CAM
[Ref.: #68373]	gamma-glutamyltransferase	-	2.3.2.2	* from API CAM
[Ref.: #68373]	L-aspartate arylamidase	+	3.4.11.21	* from API CAM
[Ref.: #68373]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API CAM
[Ref.: #68373]	urease	-	3.5.1.5	* from API CAM

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #48907]

Fatty acid	Percentage	ECL
C17:1 ISO I/C16:0 DMA	19.9	16.481
C14:0	15.2	14
C18:1 ω7c /12t/9t	12.9	17.824
C12:0	12.7	12
C16:0	11.1	16
C18:1 ω7c DMA	5.3	18.282
C16:1 ω7c	5.2	15.819
C14:0 3OH/C16:1 ISO I	4.6	15.485
C16:0 aldehyde	4	14.949
C16:0 3OH	2.5	17.52
Unidentified	1.5	16.804
C13:0 3OH/C15:1 i I/H	1	14.469
Unidentified	0.9	16.286
C13:0 ISO 2OH	0.6	13.814
C19:1 ISO I	0.5	18.473
Unidentified	0.4	13.933
C10:0	0.4	10
C18:2 ω6,9c/C18:0 ANTE	0.4	17.724
C18:0	0.3	18
Unidentified	0.2	12.471
C13:1 at 12-13	0.2	12.931
Unidentified	0.2	14.889
C13:0	0.2	13
Unidentified	0.1	14.779

Metadata FA analysis

Reference

[Ref.: #48907]

Fatty acid	Percentage	ECL
C17:1 ISO I/C16:0 DMA	22.6	16.481
C14:0	16.6	14
C18:1 ω7c /12t/9t	14.5	17.824
C12:0	13.7	12
C16:0	12.6	16
C18:1 ω7c DMA	5.8	18.282
C16:1 ω7c	4.9	15.819
C14:0 3OH/C16:1 ISO I	4.8	15.485
C16:0 aldehyde	4.5	14.949

API® CAM:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Reduction of nitrateNIT	EsteraseEST	Hippuric acidHIP	gamma-Glutamyl transferaseGGT	Reduction of Triphenyl tetrazolium chlorideTTC	Pyrrolidonyl arylamidasePYRA	L-arginine arylamidaseArgA	L-aspartic acid arylamidaseAspA	Alkaline phosphatasePAL	H2S productionH2S	Assimilation of D-glucoseGLU	Assimilation of succinateSUT	Growth inhibitionNAL	Growth inhibitionCFZ	Assimilation of acetateACE	Assimilation of propionic acidPROP	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Erythromycin resistance (+) sensitivity (-)ERO	CatalaseCAT
[Ref.: #8151]	3591	-	-	+	-	-	-	-	-	+	-	+	-	-	-	-	-	-	+/-	-	-	+
[Ref.: #8151]	3439	-	+	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	-	+
[Ref.: #8151]	3438	-	-	-	-	-	-	-	+/-	+	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8151]	Sample type/isolated from	human gingival sulcus
[Ref.: #8151]	Country	USA
[Ref.: #8151]	Country ISO 3 Code	USA
[Ref.: #8151]	Continent	North America

[Ref.: #48907]	Sample type/isolated from	Human gingival sulcus
[Ref.: #48907]	Sampling date	1977
[Ref.: #48907]	Country	USA
[Ref.: #48907]	Country ISO 3 Code	USA
[Ref.: #48907]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Human gingival sulcus
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Oral cavity and airways	#Gingiva
#Infection	#Patient	-
#Host Body-Site	#Other	#Wound

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence JX912515 (>99% sequence identity) for Campylobacter gracilis subclade from Microbeatlas

Aquatic Samples	933
Soil Samples	708
Animal Samples	47229
Plant Samples	309
Total Samples	49179

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8151]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Campylobacter gracilis gene for 16S ribosomal RNA, partial sequence, strain: JCM 8538	AB971815	1474	ENA	824 tax ID
[Ref.: #8151]	Campylobacter gracilis strain ATCC 33236 16S ribosomal RNA gene, partial sequence	JX912515	1435	ENA	824 tax ID
[Ref.: #20218]	Bacteroides gracilis, complete 16S ribosomal RNA	L04320	1450	ENA	824 tax ID	*
[Ref.: #20218]	Campylobacter gracilis strain ATCC 33236 16S ribosomal RNA (rrs) gene, partial sequence	DQ174168	1341	ENA	824 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Campylobacter gracilis strain ATCC 33236, complete genome	CP012196		ENA	824 tax ID
[Ref.: #67770]	Campylobacter gracilis NCTC12738	GCA_900446335	contig	GenBank	824 tax ID
[Ref.: #66792]	Campylobacter gracilis strain ATCC 33236	824.5	complete	PATRIC	824 tax ID	*
[Ref.: #66792]	Campylobacter gracilis strain FDAARGOS_1548	824.193	complete	PATRIC	824 tax ID	*
[Ref.: #66792]	Campylobacter gracilis ATCC 33236	2645727670	complete	JGI IMG/M	824 tax ID	*
[Ref.: #66792]	Campylobacter gracilis NCTC 12738	2808606742	draft	JGI IMG/M	824 tax ID	*

[Ref.: #8151]	GC-content	44 mol%
[Ref.: #67770]	GC-content	45 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Campylobacter gracilis ATCC 33236 NCBI: GCA_001190745.1
[Ref.: #69480]	gram-positive	no	99.992	no
[Ref.: #69480]	anaerobic	no	78.235	yes
[Ref.: #69480]	spore-forming	no	99.999	no
[Ref.: #69480]	thermophile	no	99.934	yes
[Ref.: #69480]	motile	yes	63.681	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Campylobacter gracilis ATCC 33236 NCBI: GCA_001190745.1
[Ref.: #69481]	spore-forming	no	82	no

Availability in culture collections External links

[Ref.: #8151]

Culture collection no.

DSM 19528, ATCC 33236, JCM 8538, CCUG 27720, NCTC 12738, FDC 1084, KCTC 15224

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Campylobacter gracilis ATCC 33236T.	Miller WG, Yee E	Genome Announc	10.1128/genomeA.01087-15	2015	*
Phylogeny	Comparison of strains of gram-negative, anaerobic, agar-corroding rods isolated from soft tissue infections in cats and dogs with type strains of Bacteroides gracilis, Wolinella recta, Wolinella succinogenes, and Campylobacter concisus.	Love DN, Jones RF, Bailey M, Calverley A	J Clin Microbiol	10.1128/jcm.20.4.747-750.1984	1984	*

References

#8151

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19528

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#48907 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 27720

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68373

Automatically annotated from API CAM .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Campylobacter gracilis ATCC 33236

Campylobacter gracilis ATCC 33236

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