Ralstonia insidiosa (DSM 17714, CCM 7174, CCUG 46789)

Name: Ralstonia insidiosa (DSM 17714, CCM 7174, CCUG 46789)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 2089

Type strain

Culture col. no. DSM 17714 CCM 7174 CCUG 46789 CIP 108091 LMG 21421

NCBI tax ID(s) 190721

Special Collections DSMZ CCUG CIP

History DSMZ <- CCUG <- T. Coenye, LMG <- J. LiPuma, Ann Arbor, USA <- D. Shelly, Philadelphia, USA CIP <- 2003, CCUG <- LMG <- T. Coenye <- J. LiPuma, Ann Arbor, USA <- D. Shelly, Philadelphia, USA

Links

Ralstonia insidiosa DSM 17714 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from human sputum of patient with leukemia.

Gram-negative rod-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 94678

LMG 21421,CCUG 46789,CIP 108091,CCM 7174,DSM 17714

@ref 20215

Last LPSN update 2026-05-29 08:25:24

Domain Bacteria

Phylum Pseudomonadota

Class Betaproteobacteria

Order Burkholderiales

Family Burkholderiaceae

Genus Ralstonia

Species Ralstonia insidiosa

Full scientific name Ralstonia insidiosa Coenye et al. 2003

BacDive ID	Other strains from **Ralstonia insidiosa** (18)	Type strain
135388	R. insidiosa CIP 104062, ATCC 49129
149956	R. insidiosa CCUG 38754, LMG 18101
150082	R. insidiosa CCUG 38964
150903	R. insidiosa CCUG 42677
152251	R. insidiosa CCUG 46212
152252	R. insidiosa CCUG 46213
152304	R. insidiosa CCUG 46387
152305	R. insidiosa CCUG 46388
152306	R. insidiosa CCUG 46389
152607	R. insidiosa CCUG 47187
152703	R. insidiosa CCUG 47416
152706	R. insidiosa CCUG 47426
154008	R. insidiosa CCUG 51942
154110	R. insidiosa CCUG 52392
154791	R. insidiosa CCUG 55194
155484	R. insidiosa CCUG 57845
155994	R. insidiosa CCUG 59950
175155	R. insidiosa 114805, DSM 114805

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120135	negative	rod-shaped
125439	negative		90.071
125438	negative		97.5

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7156	NUTRIENT AGAR (DSMZ Medium 1)	Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
38255	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120135	CIP Medium 328	Medium recipe at CIP
120135	CIP Medium 72	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
7156	positive	growth	28
38255	positive	growth	37
57347	positive	growth	30-42
120135	positive	growth	10-37
120135	negative	growth	5
120135	negative	growth	41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
57347	aerobe
120135	obligate aerobe
125439	obligate aerobe	97.9

Spore formation

@ref	Spore formation	Confidence
125439		99.29

Halophily

@ref	Salt	Growth	Tested relation	Concentration
120135	NaCl	positive	growth	0-2 %
120135	NaCl		growth	4 %
120135	NaCl		growth	6 %
120135	NaCl		growth	8 %
120135	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	+	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
120135	16947 ChEBI	citrate	+	carbon source
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
120135	4853 ChEBI	esculin	-	hydrolysis
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
120135	17632 ChEBI	nitrate	-	reduction
120135	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
120135	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
120135	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE
120135	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120135	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
120135	amylase	-
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
120135	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120135	caseinase	-	3.4.21.50
120135	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
120135	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
120135	gelatinase	-
120135	lecithinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
120135	lipase	+
68382	lipase (C 14)	+		from API zym
120135	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120135	ornithine decarboxylase	-	4.1.1.17
120135	oxidase	+
120135	protease	+
68382	trypsin	-	3.4.21.4	from API zym
120135	tryptophan deaminase	-
120135	tween esterase	+
120135	urease	+	3.5.1.5
68369	urease	+	3.5.1.5	from API 20NE
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120135	-	+	+	+	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
7156	-	-	-	-	+	-	-	-	+	-	-	-	-	-	+	+	+	+	+	+	+
7156	-	-	+	-	+	-	+	-	+	-	-	-	-	-	+	+	+	+	+	+	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
120135	not determinedn.d.	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	+	+	+	-	+	+	-	-	-	+	+	+	-	+	+	+	+	+	+	-	-	-	-	-	+	-	+	+	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Patient	-
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent
7156	human sputum of patient with leukemia	Homo sapiens	USA	USA	North America
57347	Human sputum,patient with leukemia	Homo sapiens	USA	USA	North America
120135	Human, Sputum	Homo sapiens	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AF488779 (>99% sequence identity) for Ralstonia insidiosa from Microbeatlas

Aquatic Samples	6363
Soil Samples	3681
Animal Samples	18388
Plant Samples	4759
Total Samples	33191

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
7156	yes, in single cases	1	Risk group (German classification) According to TRBA 466
120135		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM880140v1 assembly for Ralstonia insidiosa CCUG 46789	contig	GCA_008801405	190721.30	8115762638	190721	76.67

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Ralstonia insidiosa 16S rRNA gene, ITS1 and 23S rRNA gene, strain LMG21421	AM501946	826		190721
7156	Ralstonia sp. AU2944 16S ribosomal RNA gene, partial sequence	AF488779	1436		190721

GC content

7156

GC-content (mol%)64

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM880140v1 assembly for Ralstonia insidiosa CCUG 46789
NCBI: GCA_008801405

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.90	no
125439	gram_stain	BacteriaNetⓘ	negative	90.07	no
125439	motility	BacteriaNetⓘ	yes	72.31	no
125439	spore_formation	BacteriaNetⓘ	no	99.29	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ralstonia insidiosa strain CCUG 46789
PATRIC: 190721.30

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	97.50	no
125438	anaerobic	anaerobicⓘ	no	98.00	yes
125438	spore-forming	spore-formingⓘ	no	89.84	no
125438	thermophilic	thermophileⓘ	no	98.50	no
125438	aerobic	aerobicⓘ	yes	86.86	yes
125438	flagellated	motile2+ⓘ	yes	83.96	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.	Coenye T, Goris J, De Vos P, Vandamme P, LiPuma JJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02555-0	2003

References

#7156	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17714
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#38255	; Curators of the CIP;
#57347	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46789
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120135	Collection of Institut Pasteur ; Curators of the CIP; CIP 108091
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Ralstonia insidiosa (DSM 17714, CCM 7174, CCUG 46789)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_008801405

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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