Name: Ralstonia insidiosa DSM 17714
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2089

Type strain:

Species: Ralstonia insidiosa

Culture col. no.: DSM 17714, CCM 7174, CCUG 46789, CIP 108091, LMG 21421

Strain history: CIP <- 2003, CCUG <- LMG <- T. Coenye <- J. LiPuma, Ann Arbor, USA <- D. Shelly, Philadelphia, USA

NCBI tax ID(s): 190721 (species)

SI-ID 94678

LMG 21421, CCUG 46789, CIP 108091, CCM 7174, DSM 17714

Special Collections

Other strains from Ralstonia insidiosa

Section

Ralstonia insidiosa DSM 17714 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from human sputum of patient with leukemia.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Ralstonia*
	Species	**Ralstonia insidiosa**
	Full Scientific Name (LPSN)	Ralstonia insidiosa Coenye et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #120135]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %97.5

[Ref.: #120135]	Cell shape	rod-shaped

[Ref.: #120135]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7156]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #7156]

Culture medium growth

[Ref.: #7156]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7156]

Culture medium composition

expand / minimize

[Ref.: #38255]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38255]

Culture medium growth

[Ref.: #38255]

Culture medium composition

expand / minimize

[Ref.: #120135]

Culture medium

CIP Medium 328

[Ref.: #120135]

Culture medium growth

[Ref.: #120135]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #120135]

Culture medium

CIP Medium 72

[Ref.: #120135]

Culture medium growth

[Ref.: #120135]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7156]		growth	28 °C
[Ref.: #38255]		growth	37 °C
[Ref.: #57347]		growth	30-42 °C
[Ref.: #120135]		growth	10-37 °C
[Ref.: #120135]	no	growth	5 °C
[Ref.: #120135]	no	growth	41 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #57347]	Oxygen tolerance	aerobe
[Ref.: #120135]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #120135]	NaCl		growth	0-2	%
[Ref.: #120135]	NaCl	no	growth	4	%
[Ref.: #120135]	NaCl	no	growth	6	%
[Ref.: #120135]	NaCl	no	growth	8	%
[Ref.: #120135]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #120135]	citrate ChEBI	+	carbon source
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #120135]	esculin ChEBI	-	hydrolysis
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #120135]	nitrate ChEBI	-	reduction
[Ref.: #120135]	nitrate ChEBI	-	respiration
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #120135]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	+	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120135]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120135]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #120135]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120135]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #120135]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API 20NE
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120135]	caseinase	-	3.4.21.50
[Ref.: #120135]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #120135]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120135]	gelatinase	-
[Ref.: #120135]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #120135]	lipase	+
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #120135]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120135]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120135]	oxidase	+
[Ref.: #120135]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #120135]	tryptophan deaminase	-
[Ref.: #120135]	tween esterase	+
[Ref.: #120135]	urease	+	3.5.1.5
[Ref.: #68369]	urease	+	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #7156]	27976	-	-	-	-	+	-	-	-	+	-	-	-	-	-	+	+	+	+	+	+	+
[Ref.: #7156]	25995	-	-	+	-	+	-	+	-	+	-	-	-	-	-	+	+	+	+	+	+	+

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #120135]	2574	not determinedn.d.	+	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	+	-	+	+	+	-	+	+	-	-	-	+	+	+	-	+	+	+	+	+	+	-	-	-	-	-	+	-	+	+	+	-	+	+	+	+	+	+	-	+	+	+	+	+	+	+	+	+	+	+	+	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120135]	10875	-	+	+	+	+	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7156]	Sample type/isolated from	human sputum of patient with leukemia
[Ref.: #7156]	Country	USA
[Ref.: #7156]	Country ISO 3 Code	USA
[Ref.: #7156]	Continent	North America

[Ref.: #57347]	Sample type/isolated from	Human sputum,patient with leukemia
[Ref.: #57347]	Country	USA
[Ref.: #57347]	Country ISO 3 Code	USA
[Ref.: #57347]	Continent	North America

[Ref.: #120135]	Sample type/isolated from	Human, Sputum
[Ref.: #120135]	Country	United States of America
[Ref.: #120135]	Country ISO 3 Code	USA
[Ref.: #120135]	Continent	North America
* marker position based on {}

Isolation sources categories

#Infection	#Patient	-
#Host	#Human	-
#Host Body Product	#Fluids	#Sputum

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF488779 (>99% sequence identity) for Ralstonia insidiosa from Microbeatlas

Aquatic Samples	6363
Soil Samples	3681
Animal Samples	18388
Plant Samples	4759
Total Samples	33191

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7156]	Pathogenicity (human)	yes, in single cases
[Ref.: #7156]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120135]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7156]	Ralstonia sp. AU2944 16S ribosomal RNA gene, partial sequence	AF488779	1436	GenBank ENA	190721 tax ID
[Ref.: #20218]	Ralstonia insidiosa 16S rRNA gene, ITS1 and 23S rRNA gene, strain LMG21421	AM501946	826	GenBank ENA	190721 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ralstonia insidiosa CCUG 46789	GCA_008801405	contig	GenBank ENA	190721 tax ID	*
[Ref.: #66792]	Ralstonia insidiosa strain CCUG 46789	190721.30	wgs	PATRIC	190721 tax ID	*

[Ref.: #7156]

GC-content

64 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ralstonia insidiosa strain ATCC 49129 PATRIC: 190721.6
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ralstonia insidiosa strain CCUG 46789 PATRIC: 190721.30
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	97.5	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.999	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.839	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	98.5	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	86.86	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	83.961	no

Availability in culture collections External links

[Ref.: #7156]

Culture collection no.

DSM 17714, CCM 7174, CCUG 46789, CIP 108091, LMG 21421

[Ref.: #71736]

SI-ID 94678

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.	Coenye T, Goris J, De Vos P, Vandamme P, LiPuma JJ	Int J Syst Evol Microbiol	10.1099/ijs.0.02555-0	2003	*

References

#7156

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17714

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38255 ; Curators of the CIP;
#57347 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 46789

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71736

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID94678.1 )

#120135 Collection of Institut Pasteur ; Curators of the CIP; CIP 108091
* These data were automatically processed and therefore are not curated

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Search for species Ralstonia insidiosa in external resources:
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Ralstonia insidiosa strain ATCC 49129

Ralstonia insidiosa strain CCUG 46789

Change proposal

Change proposal