Strain identifier

BacDive ID: 2089

Type strain: Yes

Species: Ralstonia insidiosa

Strain history: CIP <- 2003, CCUG <- LMG <- T. Coenye <- J. LiPuma, Ann Arbor, USA <- D. Shelly, Philadelphia, USA

NCBI tax ID(s): 190721 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 7156

BacDive-ID: 2089

DSM-Number: 17714

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, motile, human pathogen

description: Ralstonia insidiosa DSM 17714 is an aerobe, mesophilic, motile human pathogen that was isolated from human sputum of patient with leukemia.

NCBI tax id

  • NCBI tax id: 190721
  • Matching level: species

strain history

@refhistory
7156<- CCUG <- T. Coenye, LMG <- J. LiPuma, Ann Arbor, USA <- D. Shelly, Philadelphia, USA
120135CIP <- 2003, CCUG <- LMG <- T. Coenye <- J. LiPuma, Ann Arbor, USA <- D. Shelly, Philadelphia, USA

doi: 10.13145/bacdive2089.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Ralstonia
  • species: Ralstonia insidiosa
  • full scientific name: Ralstonia insidiosa Coenye et al. 2003

@ref: 7156

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Ralstonia

species: Ralstonia insidiosa

full scientific name: Ralstonia insidiosa Coenye et al. 2003 emend. Vaneechoutte et al. 2004

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480yes95.007
6948099.982negative
120135nonegativerod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7156NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
38255MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120135CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328
120135CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
7156positivegrowth28mesophilic
38255positivegrowth37mesophilic
57347positivegrowth30-42
120135positivegrowth10-37
120135nogrowth5psychrophilic
120135nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57347aerobe
120135obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

halophily

@refsaltgrowthtested relationconcentration
120135NaClpositivegrowth0-2 %
120135NaClnogrowth4 %
120135NaClnogrowth6 %
120135NaClnogrowth8 %
120135NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836927897tryptophan-energy source
6836917632nitrate-reduction
12013516947citrate+carbon source
1201354853esculin-hydrolysis
12013517632nitrate-reduction
12013516301nitrite-reduction
12013517632nitrate-respiration

antibiotic resistance

  • @ref: 120135
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12013535581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
120135oxidase+
120135beta-galactosidase-3.2.1.23
120135alcohol dehydrogenase-1.1.1.1
120135gelatinase-
120135amylase-
120135DNase-
120135caseinase-3.4.21.50
120135catalase+1.11.1.6
120135tween esterase+
120135lecithinase+
120135lipase+
120135lysine decarboxylase-4.1.1.18
120135ornithine decarboxylase-4.1.1.17
120135protease+
120135tryptophan deaminase-
120135urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120135-++++++---++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
7156----+---+-----+++++++
7156--+-+-+-+-----+++++++

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120135+++-------+--------------------+-----------+---+-+++-++---+++-++++++-----+-+++-++++++-+++++++++++++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7156human sputum of patient with leukemiaUSAUSANorth America
57347Human sputum,patient with leukemiaUSAUSANorth America
120135Human, SputumUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Infection#Patient
#Host#Human
#Host Body Product#Fluids#Sputum

taxonmaps

  • @ref: 69479
  • File name: preview.99_714.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_51;97_54;98_59;99_714&stattab=map
  • Last taxonomy: Ralstonia insidiosa
  • 16S sequence: AF488779
  • Sequence Identity:
  • Total samples: 33191
  • soil counts: 3681
  • aquatic counts: 6363
  • animal counts: 18388
  • plant counts: 4759

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
7156yes, in single cases1Risk group (German classification)
1201351Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Ralstonia insidiosa 16S rRNA gene, ITS1 and 23S rRNA gene, strain LMG21421AM501946826ena190721
7156Ralstonia sp. AU2944 16S ribosomal RNA gene, partial sequenceAF4887791436ena190721

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ralstonia insidiosa CCUG 46789GCA_008801405contigncbi190721
66792Ralstonia insidiosa strain CCUG 46789190721.30wgspatric190721

GC content

  • @ref: 7156
  • GC-content: 64

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileyes90.192no
gram-positiveno98.699no
anaerobicno99yes
aerobicyes91.867yes
halophileno93.277no
spore-formingno93.989no
glucose-utilyes82.016yes
flagellatedyes61.65no
thermophileno99.339no
glucose-fermentno90.1no

External links

@ref: 7156

culture collection no.: DSM 17714, CCM 7174, CCUG 46789, CIP 108091, LMG 21421

straininfo link

  • @ref: 71736
  • straininfo: 94678

literature

  • topic: Phylogeny
  • Pubmed-ID: 12892129
  • title: Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.
  • authors: Coenye T, Goris J, De Vos P, Vandamme P, LiPuma JJ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02555-0
  • year: 2003
  • mesh: Base Composition, Cystic Fibrosis/microbiology, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Environmental Microbiology, Gram-Negative Aerobic Rods and Cocci/*classification/genetics/*isolation & purification/metabolism, Humans, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Terminology as Topic
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7156Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17714)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17714
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38255Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5675
57347Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46789)https://www.ccug.se/strain?id=46789
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71736Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID94678.1StrainInfo: A central database for resolving microbial strain identifiers
120135Curators of the CIPCollection of Institut Pasteur (CIP 108091)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108091