Name: Polynucleobacter cosmopolitanus MWH-MoIso2
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 2076

Type strain:

Species: Polynucleobacter cosmopolitanus

Strain Designation: MWH-MoIso2

Culture col. no.: DSM 21490, CIP 109840, LMG 25212

Strain history: CIP <- 2008, DSMZ <- M.W. Hahn, Austrian Science Academy, Mondsee, Austria : strain MWH-MoIso2

NCBI tax ID(s): 351345 (species)

SI-ID 366455

CIP 109840, DSM 21490, LMG 25212

Special Collections

Other strains from Polynucleobacter cosmopolitanus

Section

Polynucleobacter cosmopolitanus MWH-MoIso2 is an aerobe, chemoorganotroph, rod-shaped bacterium that forms circular colonies and was isolated from lake water.

colony-forming
rod-shaped
aerobe
chemoorganotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Burkholderiaceae*
	Genus	*Polynucleobacter*
	Species	**Polynucleobacter cosmopolitanus**
	Full Scientific Name (LPSN)	Polynucleobacter cosmopolitanus Hahn et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #118763]	Gram stain	negative

[Ref.: #118763]	Cell shape	rod-shaped
[Ref.: #23163]	Cell shape	rod-shaped

[Ref.: #118763]	Motility	no
[Ref.: #23163]	Motility	no

[Ref.: #23163]	Cell length	0.5-1.4 µm

[Ref.: #23163]	Cell width	0.3-0.5 µm

[Ref.: #23163]	Colony size	1 mm
[Ref.: #23163]	Colony shape	circular
[Ref.: #23163]	Incubation period	5-10 days
[Ref.: #23163]	Cultivation medium used	NSY agar

[Ref.: #23163]	Pigment production	no
[Ref.: #23163]	Pigment name	no pigments

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15741]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15741]

Culture medium growth

[Ref.: #15741]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15741]

Culture medium composition

expand / minimize

[Ref.: #23163]

Culture medium

NSY agar

[Ref.: #23163]

Culture medium growth

[Ref.: #33329]

Culture medium

MEDIUM 706- for Polynucleobacter

[Ref.: #33329]

Culture medium growth

[Ref.: #118763]

Culture medium

CIP Medium 706

[Ref.: #118763]

Culture medium growth

[Ref.: #118763]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #15741]	growth	28 °C
[Ref.: #23163]	growth	10-35 °C
[Ref.: #23163]	maximum	35 °C
[Ref.: #23163]	minimum	5 °C
[Ref.: #33329]	growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23163]

Oxygen tolerance

aerobe

[Ref.: #23163]

Nutrition type

chemoorganotroph

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23163]		NaCl		growth	0	%
[Ref.: #23163]		NaCl		maximum	0.3-0.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23163]	4-oxopentanoate ChEBI	+	growth
[Ref.: #23163]	acetate ChEBI	+	carbon source
[Ref.: #23163]	betaine ChEBI	-	carbon source
[Ref.: #23163]	betaine ChEBI	-	growth
[Ref.: #23163]	citrate ChEBI	-	growth
[Ref.: #23163]	D-fructose ChEBI	-	carbon source
[Ref.: #23163]	D-fructose ChEBI	-	growth
[Ref.: #23163]	D-fucose ChEBI	-	carbon source
[Ref.: #23163]	D-fucose ChEBI	-	growth
[Ref.: #23163]	D-galactose ChEBI	+	carbon source
[Ref.: #23163]	D-galactose ChEBI	+	growth
[Ref.: #23163]	D-galacturonic acid ChEBI	+	carbon source
[Ref.: #23163]	D-galacturonic acid ChEBI	+	growth
[Ref.: #23163]	D-glucose ChEBI	-	growth
[Ref.: #23163]	D-lyxose ChEBI	-	carbon source
[Ref.: #23163]	D-lyxose ChEBI	-	growth
[Ref.: #23163]	D-mannose ChEBI	-	growth
[Ref.: #23163]	D-sorbitol ChEBI	-	carbon source
[Ref.: #23163]	D-sorbitol ChEBI	-	growth
[Ref.: #23163]	formate ChEBI	-	carbon source
[Ref.: #23163]	formate ChEBI	-	growth
[Ref.: #23163]	fumarate ChEBI	+	carbon source
[Ref.: #23163]	fumarate ChEBI	+	growth
[Ref.: #23163]	glycolate ChEBI	-	carbon source
[Ref.: #23163]	glyoxylate ChEBI	-	growth
[Ref.: #23163]	L-alanine ChEBI	+	carbon source
[Ref.: #23163]	L-alanine ChEBI	+	growth
[Ref.: #23163]	L-asparagine ChEBI	-	carbon source
[Ref.: #23163]	L-asparagine ChEBI	-	growth
[Ref.: #23163]	L-aspartate ChEBI	-	carbon source
[Ref.: #23163]	L-aspartate ChEBI	-	growth
[Ref.: #23163]	L-cysteine ChEBI	+	carbon source
[Ref.: #23163]	L-cysteine ChEBI	+	growth
[Ref.: #23163]	L-glutamate ChEBI	-	growth
[Ref.: #23163]	L-serine ChEBI	-	carbon source
[Ref.: #23163]	malate ChEBI	+	carbon source
[Ref.: #23163]	malate ChEBI	+	growth
[Ref.: #23163]	malonate ChEBI	-	growth
[Ref.: #118763]	nitrate ChEBI	-	reduction
[Ref.: #118763]	nitrite ChEBI	-	reduction
[Ref.: #23163]	oxalate ChEBI	-	carbon source
[Ref.: #23163]	oxaloacetate ChEBI	+	carbon source
[Ref.: #23163]	oxaloacetate ChEBI	+	growth
[Ref.: #23163]	propionate ChEBI	+	carbon source
[Ref.: #23163]	propionate ChEBI	+	growth
[Ref.: #23163]	pyruvate ChEBI	+	carbon source
[Ref.: #23163]	succinate ChEBI	+	carbon source
[Ref.: #23163]	thiosulfate ChEBI	+	growth
[Ref.: #23163]	urea ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #118763]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118763]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118763]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118763]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118763]	12151	-	-	-	-	-	+	-	-	-	-	-	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15741]	Sample type/isolated from	lake water
[Ref.: #15741]	Geographic location (country and/or sea, region)	Lake Mondsee
[Ref.: #15741]	Country	Austria
[Ref.: #15741]	Country ISO 3 Code	AUT
[Ref.: #15741]	Continent	Europe

[Ref.: #23163]	Sample type/isolated from	water column (pelagic zone)

[Ref.: #118763]	Sample type/isolated from	Environment, Water, lake
[Ref.: #118763]	Geographic location (country and/or sea, region)	Mondsee
[Ref.: #118763]	Country	Austria
[Ref.: #118763]	Country ISO 3 Code	AUT
[Ref.: #118763]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Lake (large)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15741]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118763]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15741]	Polynucleobacter cosmopolitanus partial 16S rRNA gene and ITS1, strain MWH-MoIso2	AJ550672	2016	GenBank ENA	351345 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Polynucleobacter cosmopolitanus MWH-MoIso2	GCA_002224225	contig	GenBank ENA	351345 tax ID	*
[Ref.: #66792]	Polynucleobacter cosmopolitanus MWH-MoIso2	2642422582	complete	JGI IMG/M	351345 tax ID	*
[Ref.: #66792]	Polynucleobacter cosmopolitanus MWH-MoIso2	2773857926	complete	JGI IMG/M	351345 tax ID	*

[Ref.: #15741]	GC-content	44.9 mol%
[Ref.: #23163]	GC-content	44.9 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #15741]

Culture collection no.

DSM 21490, CIP 109840, LMG 25212

[Ref.: #71723]

SI-ID 366455

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Reclassification of a Polynucleobacter cosmopolitanus strain isolated from tropical Lake Victoria as Polynucleobacter victoriensis sp. nov.	Hahn MW, Schmidt J, Asiyo GS, Kyrpides NC, Woyke T, Whitman WB	Int J Syst Evol Microbiol	10.1099/ijsem.0.002421	2017	*
Phylogeny	Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting freshwater lakes and rivers.	Hahn MW, Lang E, Brandt U, Lunsdorf H, Wu QL, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.010595-0	2009	*

References

#15741

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21490

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23163

Martin W. Hahn, Elke Lang, Ulrike Brandt, Heinrich Lünsdorf, Qinglong L. Wu, Erko Stackebrandt: Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting freshwater lakes and rivers. IJSEM 60: 166 - 173 2010 ( DOI 10.1099/ijs.0.010595-0 , PubMed 19648339 )

#33329 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#71723

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID366455.1 )

#118763 Collection of Institut Pasteur ; Curators of the CIP; CIP 109840
* These data were automatically processed and therefore are not curated

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