Strain identifier

BacDive ID: 2076

Type strain: Yes

Species: Polynucleobacter cosmopolitanus

Strain Designation: MWH-MoIso2

Strain history: CIP <- 2008, DSMZ <- M.W. Hahn, Austrian Science Academy, Mondsee, Austria : strain MWH-MoIso2

NCBI tax ID(s): 351345 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 15741

BacDive-ID: 2076

DSM-Number: 21490

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganotroph, mesophilic, rod-shaped, colony-forming

description: Polynucleobacter cosmopolitanus MWH-MoIso2 is an aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from lake water.

NCBI tax id

  • NCBI tax id: 351345
  • Matching level: species

strain history

@refhistory
15741<- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MoIso2
118763CIP <- 2008, DSMZ <- M.W. Hahn, Austrian Science Academy, Mondsee, Austria : strain MWH-MoIso2

doi: 10.13145/bacdive2076.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Polynucleobacter
  • species: Polynucleobacter cosmopolitanus
  • full scientific name: Polynucleobacter cosmopolitanus Hahn et al. 2010

@ref: 15741

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Polynucleobacter

species: Polynucleobacter cosmopolitanus

full scientific name: Polynucleobacter cosmopolitanus Hahn et al. 2010

strain designation: MWH-MoIso2

type strain: yes

Morphology

cell morphology

@refcell lengthcell widthcell shapemotilitygram stain
231630.5-1.4 µm0.3-0.5 µmrod-shapedno
118763rod-shapednonegative

colony morphology

  • @ref: 23163
  • colony size: 1 mm
  • colony shape: circular
  • incubation period: 5-10 days
  • medium used: NSY agar

pigmentation

  • @ref: 23163
  • production: no
  • name: no pigments

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15741R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
23163NSY agaryes
33329MEDIUM 706- for Polynucleobacteryes
118763CIP Medium 706yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=706

culture temp

@refgrowthtypetemperaturerange
15741positivegrowth28mesophilic
23163positivegrowth10-35
23163positivemaximum35mesophilic
23163positiveminimum5psychrophilic
33329positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 23163
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 23163
  • type: chemoorganotroph

halophily

@refsaltgrowthtested relationconcentration
23163NaClpositivegrowth0 %
23163NaClpositivemaximum0.3-0.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2316329991L-aspartate-carbon source
2316317750betaine-carbon source
2316315824D-fructose-carbon source
2316328847D-fucose-carbon source
2316362318D-lyxose-carbon source
2316317924D-sorbitol-carbon source
2316315740formate-carbon source
2316329805glycolate-carbon source
2316317196L-asparagine-carbon source
2316317115L-serine-carbon source
2316330623oxalate-carbon source
2316329991L-aspartate-growth
2316329985L-glutamate-growth
2316317750betaine-growth
2316316947citrate-growth
2316315824D-fructose-growth
2316328847D-fucose-growth
2316317634D-glucose-growth
2316362318D-lyxose-growth
2316316024D-mannose-growth
2316317924D-sorbitol-growth
2316315740formate-growth
2316336655glyoxylate-growth
2316317196L-asparagine-growth
2316315792malonate-growth
2316330089acetate+carbon source
2316312936D-galactose+carbon source
2316318024D-galacturonic acid+carbon source
2316329806fumarate+carbon source
2316316977L-alanine+carbon source
2316317561L-cysteine+carbon source
2316325115malate+carbon source
2316316452oxaloacetate+carbon source
2316317272propionate+carbon source
2316315361pyruvate+carbon source
2316330031succinate+carbon source
2316312936D-galactose+growth
2316318024D-galacturonic acid+growth
2316329806fumarate+growth
2316316977L-alanine+growth
2316317561L-cysteine+growth
23163391504-oxopentanoate+growth
2316325115malate+growth
2316316452oxaloacetate+growth
2316317272propionate+growth
2316316094thiosulfate+growth
2316316199urea+growth
11876317632nitrate-reduction
11876316301nitrite-reduction

metabolite production

  • @ref: 118763
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)-
68382alkaline phosphatase-3.1.3.1
118763oxidase-
118763catalase-1.11.1.6
118763urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118763-----+-----+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
15741lake waterLake MondseeAustriaAUTEurope
23163water column (pelagic zone)
118763Environment, Water, lakeMondseeAustriaAUTEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Lake (large)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
157411Risk group (German classification)
1187631Risk group (French classification)

Sequence information

16S sequences

  • @ref: 15741
  • description: Polynucleobacter cosmopolitanus partial 16S rRNA gene and ITS1, strain MWH-MoIso2
  • accession: AJ550672
  • length: 2016
  • database: ena
  • NCBI tax ID: 351345

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Polynucleobacter cosmopolitanus MWH-MoIso2GCA_002224225contigncbi351345
66792Polynucleobacter cosmopolitanus MWH-MoIso22642422582completeimg351345
66792Polynucleobacter cosmopolitanus MWH-MoIso22773857926completeimg351345

GC content

@refGC-contentmethod
1574144.9
2316344.9high performance liquid chromatography (HPLC)

External links

@ref: 15741

culture collection no.: DSM 21490, CIP 109840, LMG 25212

straininfo link

  • @ref: 71723
  • straininfo: 366455

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19648339Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting freshwater lakes and rivers.Hahn MW, Lang E, Brandt U, Lunsdorf H, Wu QL, Stackebrandt EInt J Syst Evol Microbiol10.1099/ijs.0.010595-02009Base Composition, Burkholderiaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, Plankton/*classification/genetics/*isolation & purification/metabolism, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny29068276Reclassification of a Polynucleobacter cosmopolitanus strain isolated from tropical Lake Victoria as Polynucleobacter victoriensis sp. nov.Hahn MW, Schmidt J, Asiyo GS, Kyrpides NC, Woyke T, Whitman WBInt J Syst Evol Microbiol10.1099/ijsem.0.0024212017Africa, Eastern, Bacterial Typing Techniques, Burkholderiaceae/*classification, DNA, Bacterial/genetics, Lakes/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15741Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21490)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21490
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23163Martin W. Hahn, Elke Lang, Ulrike Brandt, Heinrich Lünsdorf, Qinglong L. Wu, Erko Stackebrandt10.1099/ijs.0.010595-0Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting freshwater lakes and riversIJSEM 60: 166-173 201019648339
33329Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/7630
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
71723Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID366455.1StrainInfo: A central database for resolving microbial strain identifiers
118763Curators of the CIPCollection of Institut Pasteur (CIP 109840)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109840