Strain identifier
BacDive ID: 2076
Type strain:
Species: Polynucleobacter cosmopolitanus
Strain Designation: MWH-MoIso2
Strain history: CIP <- 2008, DSMZ <- M.W. Hahn, Austrian Science Academy, Mondsee, Austria : strain MWH-MoIso2
NCBI tax ID(s): 351345 (species)
General
@ref: 15741
BacDive-ID: 2076
DSM-Number: 21490
keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganotroph, mesophilic, rod-shaped, colony-forming
description: Polynucleobacter cosmopolitanus MWH-MoIso2 is an aerobe, chemoorganotroph, mesophilic bacterium that forms circular colonies and was isolated from lake water.
NCBI tax id
- NCBI tax id: 351345
- Matching level: species
strain history
@ref | history |
---|---|
15741 | <- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MoIso2 |
118763 | CIP <- 2008, DSMZ <- M.W. Hahn, Austrian Science Academy, Mondsee, Austria : strain MWH-MoIso2 |
doi: 10.13145/bacdive2076.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Polynucleobacter
- species: Polynucleobacter cosmopolitanus
- full scientific name: Polynucleobacter cosmopolitanus Hahn et al. 2010
@ref: 15741
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Polynucleobacter
species: Polynucleobacter cosmopolitanus
full scientific name: Polynucleobacter cosmopolitanus Hahn et al. 2010
strain designation: MWH-MoIso2
type strain: yes
Morphology
cell morphology
@ref | cell length | cell width | cell shape | motility | gram stain |
---|---|---|---|---|---|
23163 | 0.5-1.4 µm | 0.3-0.5 µm | rod-shaped | no | |
118763 | rod-shaped | no | negative |
colony morphology
- @ref: 23163
- colony size: 1 mm
- colony shape: circular
- incubation period: 5-10 days
- medium used: NSY agar
pigmentation
- @ref: 23163
- production: no
- name: no pigments
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
15741 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
23163 | NSY agar | yes | ||
33329 | MEDIUM 706- for Polynucleobacter | yes | ||
118763 | CIP Medium 706 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=706 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
15741 | positive | growth | 28 | mesophilic |
23163 | positive | growth | 10-35 | |
23163 | positive | maximum | 35 | mesophilic |
23163 | positive | minimum | 5 | psychrophilic |
33329 | positive | growth | 30 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 23163
- oxygen tolerance: aerobe
nutrition type
- @ref: 23163
- type: chemoorganotroph
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
23163 | NaCl | positive | growth | 0 % |
23163 | NaCl | positive | maximum | 0.3-0.5 %(w/v) |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
23163 | 29991 | L-aspartate | - | carbon source |
23163 | 17750 | betaine | - | carbon source |
23163 | 15824 | D-fructose | - | carbon source |
23163 | 28847 | D-fucose | - | carbon source |
23163 | 62318 | D-lyxose | - | carbon source |
23163 | 17924 | D-sorbitol | - | carbon source |
23163 | 15740 | formate | - | carbon source |
23163 | 29805 | glycolate | - | carbon source |
23163 | 17196 | L-asparagine | - | carbon source |
23163 | 17115 | L-serine | - | carbon source |
23163 | 30623 | oxalate | - | carbon source |
23163 | 29991 | L-aspartate | - | growth |
23163 | 29985 | L-glutamate | - | growth |
23163 | 17750 | betaine | - | growth |
23163 | 16947 | citrate | - | growth |
23163 | 15824 | D-fructose | - | growth |
23163 | 28847 | D-fucose | - | growth |
23163 | 17634 | D-glucose | - | growth |
23163 | 62318 | D-lyxose | - | growth |
23163 | 16024 | D-mannose | - | growth |
23163 | 17924 | D-sorbitol | - | growth |
23163 | 15740 | formate | - | growth |
23163 | 36655 | glyoxylate | - | growth |
23163 | 17196 | L-asparagine | - | growth |
23163 | 15792 | malonate | - | growth |
23163 | 30089 | acetate | + | carbon source |
23163 | 12936 | D-galactose | + | carbon source |
23163 | 18024 | D-galacturonic acid | + | carbon source |
23163 | 29806 | fumarate | + | carbon source |
23163 | 16977 | L-alanine | + | carbon source |
23163 | 17561 | L-cysteine | + | carbon source |
23163 | 25115 | malate | + | carbon source |
23163 | 16452 | oxaloacetate | + | carbon source |
23163 | 17272 | propionate | + | carbon source |
23163 | 15361 | pyruvate | + | carbon source |
23163 | 30031 | succinate | + | carbon source |
23163 | 12936 | D-galactose | + | growth |
23163 | 18024 | D-galacturonic acid | + | growth |
23163 | 29806 | fumarate | + | growth |
23163 | 16977 | L-alanine | + | growth |
23163 | 17561 | L-cysteine | + | growth |
23163 | 39150 | 4-oxopentanoate | + | growth |
23163 | 25115 | malate | + | growth |
23163 | 16452 | oxaloacetate | + | growth |
23163 | 17272 | propionate | + | growth |
23163 | 16094 | thiosulfate | + | growth |
23163 | 16199 | urea | + | growth |
118763 | 17632 | nitrate | - | reduction |
118763 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 118763
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | valine arylamidase | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
68382 | esterase (C 4) | - | |
68382 | alkaline phosphatase | - | 3.1.3.1 |
118763 | oxidase | - | |
118763 | catalase | - | 1.11.1.6 |
118763 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
118763 | - | - | - | - | - | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
15741 | lake water | Lake Mondsee | Austria | AUT | Europe |
23163 | water column (pelagic zone) | ||||
118763 | Environment, Water, lake | Mondsee | Austria | AUT | Europe |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Lake (large)
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
15741 | 1 | Risk group (German classification) |
118763 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 15741
- description: Polynucleobacter cosmopolitanus partial 16S rRNA gene and ITS1, strain MWH-MoIso2
- accession: AJ550672
- length: 2016
- database: ena
- NCBI tax ID: 351345
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Polynucleobacter cosmopolitanus MWH-MoIso2 | GCA_002224225 | contig | ncbi | 351345 |
66792 | Polynucleobacter cosmopolitanus MWH-MoIso2 | 2642422582 | complete | img | 351345 |
66792 | Polynucleobacter cosmopolitanus MWH-MoIso2 | 2773857926 | complete | img | 351345 |
GC content
@ref | GC-content | method |
---|---|---|
15741 | 44.9 | |
23163 | 44.9 | high performance liquid chromatography (HPLC) |
External links
@ref: 15741
culture collection no.: DSM 21490, CIP 109840, LMG 25212
straininfo link
- @ref: 71723
- straininfo: 366455
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 19648339 | Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting freshwater lakes and rivers. | Hahn MW, Lang E, Brandt U, Lunsdorf H, Wu QL, Stackebrandt E | Int J Syst Evol Microbiol | 10.1099/ijs.0.010595-0 | 2009 | Base Composition, Burkholderiaceae/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Fresh Water/*microbiology, Molecular Sequence Data, Phylogeny, Plankton/*classification/genetics/*isolation & purification/metabolism, RNA, Ribosomal, 16S/genetics | Metabolism |
Phylogeny | 29068276 | Reclassification of a Polynucleobacter cosmopolitanus strain isolated from tropical Lake Victoria as Polynucleobacter victoriensis sp. nov. | Hahn MW, Schmidt J, Asiyo GS, Kyrpides NC, Woyke T, Whitman WB | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002421 | 2017 | Africa, Eastern, Bacterial Typing Techniques, Burkholderiaceae/*classification, DNA, Bacterial/genetics, Lakes/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
15741 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21490) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-21490 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
23163 | Martin W. Hahn, Elke Lang, Ulrike Brandt, Heinrich Lünsdorf, Qinglong L. Wu, Erko Stackebrandt | 10.1099/ijs.0.010595-0 | Polynucleobacter cosmopolitanus sp. nov., free-living planktonic bacteria inhabiting freshwater lakes and rivers | IJSEM 60: 166-173 2010 | 19648339 | |
33329 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/7630 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68382 | Automatically annotated from API zym | |||||
71723 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID366455.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
118763 | Curators of the CIP | Collection of Institut Pasteur (CIP 109840) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109840 |