Brucella rhizosphaerae (DSM 19824, CCM 7493, CCUG 55411)

Name: Brucella rhizosphaerae (DSM 19824, CCM 7493, CCUG 55411)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1894

Type strain

Strain Designation PR17

Culture col. no. DSM 19824 CCM 7493 CCUG 55411 PR17

NCBI tax ID(s) 571254

Special Collections DSMZ CCUG Literature strains

History <- P. Kämpfer; PR17 <- H. Scholz;

Links

Brucella rhizosphaerae PR17 is a mesophilic, Gram-negative, motile prokaryote that forms circular colonies and was isolated from rhizosphere .

Gram-negative motile rod-shaped colony-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 402054

DSM 19824,CCUG 55411

@ref 20215

Last LPSN update 2026-02-09 11:18:12

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Brucellaceae

Genus Brucella

Species Brucella rhizosphaerae

Full scientific name Brucella rhizosphaerae (Kämpfer et al. 2008) Hördt et al. 2020

Synonyms (1)

Ochrobactrum rhizosphaerae

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell shape	Motility	Confidence
23342	negative	2 µm	rod-shaped
125439	negative				99.3

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period
23342	2 mm	beige, translucent	circular	1 day

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8336	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23342	MacConkey
23342	Nutrient agar (NA)
23342	PYE agar
23342	Reasoner's 2A agar (R2A)
23342	Trypticase Soy Agar (TSA)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8336	positive	growth	28	mesophilic
23342		growth	25-30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	95.8

Spore formation

@ref	Spore formation	Confidence
23342
125439		98.9

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23342	37054 ChEBI	3-hydroxybutyrate	+	assimilation
23342	17879 ChEBI	4-hydroxybenzoate	-	assimilation
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23342	16958 ChEBI	beta-alanine	+	assimilation
23342	17057 ChEBI	cellobiose	+	assimilation
23342	16383 ChEBI	cis-aconitate	+	assimilation
23342	16947 ChEBI	citrate	+	assimilation
23342	15824 ChEBI	D-fructose	+	assimilation
23342	8391 ChEBI	D-gluconate	-	assimilation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23342	17924 ChEBI	D-sorbitol	+	assimilation
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
23342	16865 ChEBI	gamma-aminobutyric acid	+	assimilation
23342		gamma-L-glutamate-4-nitroanilide	-	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
23342	29991 ChEBI	L-aspartate	+	assimilation
23342	15971 ChEBI	L-histidine	+	assimilation
23342	15603 ChEBI	L-leucine	+	assimilation
23342		L-proline-4-nitroanilide	+	hydrolysis
23342	62345 ChEBI	L-rhamnose	+	assimilation
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23342	68428 ChEBI	maltitol	+	assimilation
23342	17306 ChEBI	maltose	+	assimilation
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
23342	17268 ChEBI	myo-inositol	+	assimilation
23342	28037 ChEBI	N-acetylgalactosamine	+	assimilation
23342	506227 ChEBI	N-acetylglucosamine	+/-	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
23342	91050 ChEBI	p-nitrophenyl phenyl phosphonate	+	hydrolysis
23342	15963 ChEBI	ribitol	+	assimilation
23342	9300 ChEBI	suberic acid	-	assimilation
23342	17992 ChEBI	sucrose	+	assimilation
23342	15708 ChEBI	trans-aconitate	+	assimilation
23342	27082 ChEBI	trehalose	+	assimilation
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
23342	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68369	gelatinase	-		from API 20NE
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
8336	-	-	-	-	-	-	-	-	+	+	+	+	+	+	+/-	+/-	-	+	+	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	-	-
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Sampling date	Country	Country ISO 3 Code	Continent
8336	rhizosphere (potato)		Austria	AUT	Europe
60577	Potato rhizosphere	2000	Austria	AUT	Europe

Taxonmaps

69479

Global distribution of 16S sequence AM490632 (>99% sequence identity) for Ochrobactrum from Microbeatlas

Aquatic Samples	117
Soil Samples	290
Animal Samples	411
Plant Samples	74
Total Samples	892

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8336	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM225247v1 assembly for Brucella rhizosphaerae PR17	contig	GCA_002252475	571254.3	2854869019	571254	60.8
124043	ASM4268552v1 assembly for Brucella rhizosphaerae CCM 7493	contig	GCA_042685525			571254	42.56
124043	ASM4193000v1 assembly for Brucella rhizosphaerae DSM 19824	scaffold	GCA_041930005			571254	34.79
124043	ASM4266270v1 assembly for Brucella rhizosphaerae CCM 7493	contig	GCA_042662705			571254	29.55

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8336	Ochrobactrum rhizosphaerae partial 16S rRNA gene, type strain PR17	AM490632	1387		571254
124043	Ochrobactrum rhizosphaerae strain CCM 7493 16S ribosomal RNA gene, partial sequence.	MT760146	1328		571254

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brucella rhizosphaerae PR17
NCBI: GCA_002252475

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.90	no
125439	motility	BacteriaNetⓘ	yes	77.40	no
125439	gram_stain	BacteriaNetⓘ	negative	99.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.80	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Phylogeny	Ochrobactrum rhizosphaerae sp. nov. and Ochrobactrum thiophenivorans sp. nov., isolated from the environment.	Kampfer P, Sessitsch A, Schloter M, Huber B, Busse HJ, Scholz HC	Int J Syst Evol Microbiol	10.1099/ijs.0.65407-0	2008

References

#8336	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19824
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23342	Peter Kämpfer, Angela Sessitsch, Michael Schloter, Birgit Huber,Hans-Jürgen Busse, Holger C. Scholz: Ochrobactrum rhizosphaerae sp. nov. and Ochrobactrum thiophenivorans sp. nov., isolated from the environment. IJSEM 58: 1426 - 1431 2008 ( DOI 10.1099/ijs.0.65407-0 , PubMed 18523190 )
#60577	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55411
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brucella rhizosphaerae (DSM 19824, CCM 7493, CCUG 55411)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002252475

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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