Strain identifier

BacDive ID: 1894

Type strain: Yes

Species: Brucella rhizosphaerae

Strain Designation: PR17

Strain history: <- P. Kämpfer; PR17 <- H. Scholz;

NCBI tax ID(s): 571254 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8336

BacDive-ID: 1894

DSM-Number: 19824

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped, colony-forming

description: Brucella rhizosphaerae PR17 is a mesophilic, Gram-negative, motile bacterium that forms circular colonies and was isolated from rhizosphere .

NCBI tax id

  • NCBI tax id: 571254
  • Matching level: species

strain history

  • @ref: 8336
  • history: <- P. Kämpfer; PR17 <- H. Scholz;

doi: 10.13145/bacdive1894.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Brucellaceae
  • genus: Brucella
  • species: Brucella rhizosphaerae
  • full scientific name: Brucella rhizosphaerae (Kämpfer et al. 2008) Hördt et al. 2020
  • synonyms

    • @ref: 20215
    • synonym: Ochrobactrum rhizosphaerae

@ref: 8336

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Brucellaceae

genus: Brucella

species: Brucella rhizosphaerae

full scientific name: Brucella rhizosphaerae (Kämpfer et al. 2008) Hördt et al. 2020

strain designation: PR17

type strain: yes

Morphology

cell morphology

  • @ref: 23342
  • gram stain: negative
  • cell length: 2 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 23342
  • colony size: 2 mm
  • colony color: beige, translucent
  • colony shape: circular
  • incubation period: 1 day

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8336TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23342MacConkeyyes
23342Nutrient agar (NA)yes
23342PYE agaryes
23342Reasoner's 2A agar (R2A)yes
23342Trypticase Soy Agar (TSA)yes

culture temp

@refgrowthtypetemperaturerange
8336positivegrowth28mesophilic
23342growth25-30mesophilic

Physiology and metabolism

spore formation

  • @ref: 23342
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23342178794-hydroxybenzoate-assimilation
233428391D-gluconate-assimilation
233429300suberic acid-assimilation
23342gamma-L-glutamate-4-nitroanilide-hydrolysis
2334216865gamma-aminobutyric acid+assimilation
2334215963ribitol+assimilation
2334216958beta-alanine+assimilation
2334217057cellobiose+assimilation
2334216383cis-aconitate+assimilation
2334216947citrate+assimilation
2334215824D-fructose+assimilation
2334217924D-sorbitol+assimilation
23342370543-hydroxybutyrate+assimilation
2334229991L-aspartate+assimilation
2334215971L-histidine+assimilation
2334215603L-leucine+assimilation
2334262345L-rhamnose+assimilation
2334268428maltitol+assimilation
2334217306maltose+assimilation
2334217268myo-inositol+assimilation
2334228037N-acetylgalactosamine+assimilation
2334217992sucrose+assimilation
2334215708trans-aconitate+assimilation
2334227082trehalose+assimilation
23342L-proline-4-nitroanilide+hydrolysis
2334291050p-nitrophenyl phenyl phosphonate+hydrolysis
23342506227N-acetylglucosamine+/-assimilation
6836925115malate+assimilation
6836917128adipate-assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
23342cytochrome oxidase+1.9.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
8336--------+++++++/-+/--++-+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
8336rhizosphere (potato)AustriaAUTEurope
60577Potato rhizosphereAustriaAUTEurope2000

isolation source categories

Cat1Cat2Cat3
#Environmental
#Host Body-Site#Plant#Rhizosphere

taxonmaps

  • @ref: 69479
  • File name: preview.99_5582.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_45;97_1426;98_4165;99_5582&stattab=map
  • Last taxonomy: Ochrobactrum
  • 16S sequence: AM490632
  • Sequence Identity:
  • Total samples: 892
  • soil counts: 290
  • aquatic counts: 117
  • animal counts: 411
  • plant counts: 74

Safety information

risk assessment

  • @ref: 8336
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 8336
  • description: Ochrobactrum rhizosphaerae partial 16S rRNA gene, type strain PR17
  • accession: AM490632
  • length: 1387
  • database: ena
  • NCBI tax ID: 571254

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brucella rhizosphaerae PR17GCA_002252475contigncbi571254
66792Brucella rhizosphaerae PR172854869019draftimg571254

External links

@ref: 8336

culture collection no.: DSM 19824, CCM 7493, CCUG 55411

straininfo link

  • @ref: 71538
  • straininfo: 402054

literature

  • topic: Phylogeny
  • Pubmed-ID: 18523190
  • title: Ochrobactrum rhizosphaerae sp. nov. and Ochrobactrum thiophenivorans sp. nov., isolated from the environment.
  • authors: Kampfer P, Sessitsch A, Schloter M, Huber B, Busse HJ, Scholz HC
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65407-0
  • year: 2008
  • mesh: Bacterial Typing Techniques, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Ochrobactrum/*classification/genetics/isolation & purification/physiology, Phenotype, Phylogeny, Plant Roots/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Solanum tuberosum/*microbiology, Species Specificity, Waste Disposal, Fluid/*methods
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8336Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19824)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19824
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23342Peter Kämpfer, Angela Sessitsch, Michael Schloter, Birgit Huber,Hans-Jürgen Busse, Holger C. Scholz10.1099/ijs.0.65407-0Ochrobactrum rhizosphaerae sp. nov. and Ochrobactrum thiophenivorans sp. nov., isolated from the environmentIJSEM 58: 1426-1431 200818523190
60577Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55411)https://www.ccug.se/strain?id=55411
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71538Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID402054.1StrainInfo: A central database for resolving microbial strain identifiers