Brucella cytisi (DSM 19778, CECT 7172, CIP 109590)

Name: Brucella cytisi (DSM 19778, CECT 7172, CIP 109590)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1890

Type strain

Culture col. no. DSM 19778 CECT 7172 CIP 109590 LMG 22713 CCUG 54646 1 more

NCBI tax ID(s) 407152

Special Collections DSMZ CCUG CIP Literature strains

History <- CECT; ESC1 {2007} <- E. Velázquez CIP <- 2007, CECT <- 2006, E. Velazquez, Salamanca Univ., Salamanca, Spain <- J. A. Carrasco, CIFA, Sevilla, Spain: strain ESC1

Links

Brucella cytisi DSM 19778 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from root nodules of Cytisus scoparius.

Gram-negative motile colony-forming aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 292882

LMG 22713,CECT 7172,CCUG 54646,DSM 19778,CIP 109590

@ref 20215

Last LPSN update 2026-02-09 11:18:12

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Brucellaceae

Genus Brucella

Species Brucella cytisi

Full scientific name Brucella cytisi (Zurdo-Piñeiro et al. 2007) Hördt et al. 2020

Synonyms (1)

Ochrobactrum cytisi

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
23339	negative
118836	negative	rod-shaped
125438	negative		100

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
23339	2-3 mm	white to beige	circular	1 day	YMA

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15442	VIBRIO NATRIEGENS MEDIUM (DSMZ Medium 115)	Medium recipe at MediaDive	Name: VIBRIO NATRIEGENS MEDIUM (DSMZ Medium 115) Composition: NaCl 15.0 g/l Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23339	YMA
37777	MEDIUM 328- for nutrient agar		Distilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
118836	CIP Medium 328	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
15442	positive	growth	26
37777	positive	growth	25
60279	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
60279	aerobe
118836	obligate aerobe
125439	obligate aerobe	97.9

Spore formation

@ref	Spore formation	Confidence
23339
125439		95.5
125438		91.378

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23339	16808 ChEBI	2-dehydro-D-gluconate	+	assimilation
23339	17426 ChEBI	5-dehydro-D-gluconate	+	assimilation
23339	17128 ChEBI	adipate	-	assimilation
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
23339	27613 ChEBI	amygdalin	-	assimilation
23339	22605 ChEBI	arabinitol	+	assimilation
23339	18305 ChEBI	arbutin	+	assimilation
68370	29016 ChEBI	arginine	-	hydrolysis	from API 20STR
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23339	17057 ChEBI	cellobiose	+/-	assimilation
23339	16947 ChEBI	citrate	+	assimilation
23339	17108 ChEBI	D-arabinose	+	assimilation
23339	15824 ChEBI	D-fructose	+	assimilation
23339	15824 ChEBI	D-fructose	+/-	assimilation
23339	28847 ChEBI	D-fucose	+	assimilation
23339	17634 ChEBI	D-glucose	+	assimilation
68369	17634 ChEBI	D-glucose	+	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68370	16899 ChEBI	D-mannitol	-	builds acid from	from API 20STR
23339	16024 ChEBI	D-mannose	+	assimilation
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
68370	16988 ChEBI	D-ribose	-	builds acid from	from API 20STR
68370	17924 ChEBI	D-sorbitol	-	builds acid from	from API 20STR
23339	65327 ChEBI	D-xylose	+/-	assimilation
23339	27689 ChEBI	decanoate	-	assimilation
23339	17113 ChEBI	erythritol	+	assimilation
23339	4853 ChEBI	esculin	+	hydrolysis
68370	4853 ChEBI	esculin	-	hydrolysis	from API 20STR
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
23339	16813 ChEBI	galactitol	+	assimilation
23339	16813 ChEBI	galactitol	+/-	assimilation
23339	28260 ChEBI	galactose	+/-	assimilation
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
23339	28066 ChEBI	gentiobiose	-	assimilation
23339	24265 ChEBI	gluconate	+/-	assimilation
23339	17754 ChEBI	glycerol	+/-	assimilation
23339	28087 ChEBI	glycogen	-	assimilation
68370	28087 ChEBI	glycogen	-	builds acid from	from API 20STR
68370	606565 ChEBI	hippurate	+	hydrolysis	from API 20STR
23339	15443 ChEBI	inulin	-	assimilation
68370	15443 ChEBI	inulin	-	builds acid from	from API 20STR
23339	30849 ChEBI	L-arabinose	+	assimilation
68370	30849 ChEBI	L-arabinose	-	builds acid from	from API 20STR
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
23339	18403 ChEBI	L-arabitol	-	assimilation
23339	18287 ChEBI	L-fucose	+	assimilation
23339	62345 ChEBI	L-rhamnose	+	assimilation
23339	17266 ChEBI	L-sorbose	-	assimilation
23339	65328 ChEBI	L-xylose	+/-	assimilation
23339	17716 ChEBI	lactose	+/-	assimilation
68370	17716 ChEBI	lactose	-	builds acid from	from API 20STR
23339	25097 ChEBI	lyxose	+	assimilation
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23339	17306 ChEBI	maltose	+	assimilation
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
23339	29864 ChEBI	mannitol	+	assimilation
23339	6731 ChEBI	melezitose	-	assimilation
23339	28053 ChEBI	melibiose	+/-	assimilation
23339	320061 ChEBI	methyl alpha-D-glucopyranoside	+/-	assimilation
23339	43943 ChEBI	methyl alpha-D-mannoside	-	assimilation
23339	74863 ChEBI	methyl beta-D-xylopyranoside	-	assimilation
23339	17268 ChEBI	myo-inositol	+/-	assimilation
23339	506227 ChEBI	N-acetylglucosamine	+	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
23339	17632 ChEBI	nitrate	+	reduction
118836	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
118836	16301 ChEBI	nitrite	+	reduction
23339	16634 ChEBI	raffinose	-	assimilation
68370	16634 ChEBI	raffinose	-	builds acid from	from API 20STR
23339	15963 ChEBI	ribitol	+	assimilation
23339	33942 ChEBI	ribose	+	assimilation
23339	17814 ChEBI	salicin	-	assimilation
23339	30911 ChEBI	sorbitol	-	assimilation
23339	28017 ChEBI	starch	-	assimilation
68370	28017 ChEBI	starch	-	builds acid from	from API 20STR
23339	17992 ChEBI	sucrose	+	assimilation
23339	33954 ChEBI	tagatose	+	assimilation
23339	27082 ChEBI	trehalose	+/-	assimilation
68370	27082 ChEBI	trehalose	-	builds acid from	from API 20STR
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
23339	32528 ChEBI	turanose	+	assimilation
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE
23339	17151 ChEBI	xylitol	-	assimilation

Antibiotic resistance

@ref	ChEBI	Metabolite
23339	28971	ampicillin
23339	3515	cefuroxime
23339	17698	chloramphenicol
23339	100241	ciprofloxacin
23339	49566	cloxacillin
23339	48923	erythromycin
23339	17833	gentamicin
23339	7507	neomycin
23339	27701	oxytetracycline
23339	17334	penicillin
23339	8309	polymyxin b

Metabolite production

@ref	Chebi-ID	Metabolite
23339	15688 ChEBI	acetoin
68370	15688 ChEBI	acetoin	from API 20STR
23339	35581 ChEBI	indole
68369	35581 ChEBI	indole	from API 20NE
118836	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
23339	15688 ChEBI	acetoin	+
68370	15688 ChEBI	acetoin	+		from API 20STR
68369	35581 ChEBI	indole		-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
23339	Acid phosphatase	+	3.1.3.2
68382	acid phosphatase	+	3.1.3.2	from API zym
23339	Alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68370	alkaline phosphatase	-	3.1.3.1	from API 20STR
23339	alpha-chymotrypsin	+	3.4.21.1
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
23339	alpha-fucosidase	-	3.2.1.51
68382	alpha-fucosidase	-	3.2.1.51	from API zym
23339	alpha-galactosidase	-	3.2.1.22
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68370	alpha-galactosidase	-	3.2.1.22	from API 20STR
23339	alpha-glucosidase	+	3.2.1.20
68382	alpha-glucosidase	-	3.2.1.20	from API zym
23339	alpha-mannosidase	-	3.2.1.24
68382	alpha-mannosidase	-	3.2.1.24	from API zym
23339	arginine dihydrolase	-	3.5.3.6
68370	arginine dihydrolase	-	3.5.3.6	from API 20STR
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
23339	beta-galactosidase	+/-	3.2.1.23
23339	beta-galactosidase	+	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
68370	beta-galactosidase	-	3.2.1.23	from API 20STR
23339	beta-glucosidase	-	3.2.1.21
68382	beta-glucosidase	-	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68370	beta-glucosidase	-	3.2.1.21	from API 20STR
23339	beta-glucuronidase	-	3.2.1.31
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68370	beta-glucuronidase	-	3.2.1.31	from API 20STR
118836	catalase	+	1.11.1.6
23339	cystine arylamidase	+	3.4.11.3
68382	cystine arylamidase	+	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
23339	esterase (C 4)	+
68382	esterase (C 4)	+		from API zym
23339	esterase lipase (C 8)	+
68382	esterase lipase (C 8)	-		from API zym
23339	gelatinase	-
68369	gelatinase	-		from API 20NE
23339	leucine arylamidase	+	3.4.11.1
68382	leucine arylamidase	+	3.4.11.1	from API zym
68370	leucine arylamidase	+	3.4.11.1	from API 20STR
23339	lipase (C 14)	+
68382	lipase (C 14)	-		from API zym
23339	lysine decarboxylase	-	4.1.1.18
23339	N-acetyl-beta-glucosaminidase	-	3.2.1.52
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
23339	naphthol-AS-BI-phosphohydrolase	+
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
23339	ornithine decarboxylase	-	4.1.1.17
118836	oxidase	+
68370	pyrrolidonyl arylamidase	+	3.4.19.3	from API 20STR
23339	trypsin	+	3.4.21.4
68382	trypsin	-	3.4.21.4	from API zym
23339	urease	-	3.5.1.5
118836	urease	+	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
23339	valine arylamidase	+
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118836	-	+	+	-	-	+	-	+	-	-	+	+	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
15442	+	-	-	-	-	-	-	-	+	+	+	+/-	+	+	+/-	+/-	-	+	+	-	+

API 20STR

@ref	Acetoin production (Voges Proskauer test)VP	HIP	ESC	PYRA	alpha GAL	beta GUR	beta GAL	PAL	LAP	ADH	RIB	ARA	MAN	SOR	LAC	TRE	INU	RAF	AMD	GLYG	beta hemolysis presentbeta HEM
15442	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Root nodule

Isolation

@ref	Sample type	Host species	Country	Country ISO 3 Code	Continent
15442	root nodules of Cytisus scoparius	Cytisus scoparius	Spain	ESP	Europe
60279	Nodules from Cytisus scoparius		Spain	ESP	Europe
118836	Legume Cystisus scoparius, nodules		Spain	ESP	Europe

Taxonmaps

69479

Global distribution of 16S sequence AY776289 (>99% sequence identity) for Ochrobactrum from Microbeatlas

Aquatic Samples	339
Soil Samples	432
Animal Samples	1469
Plant Samples	154
Total Samples	2394

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15442	1	Risk group (German classification) According to TRBA 466
118836	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
124043	ASM4193016v1 assembly for Brucella cytisi DSM 19778	scaffold	GCA_041930165		407152	24.5
66792	O.cytisi_LMG_22713.fasta assembly for Brucella cytisi LMG 22713	contig	GCA_012103075	407152.6	407152	9.1

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15442	Ochrobactrum cytisi strain ESC1 16S ribosomal RNA gene, partial sequence	AY776289	1476		407152

GC content

@ref	GC-content (mol%)	Method
15442	56.4	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brucella cytisi LMG 22713
NCBI: GCA_012103075

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	95.50	no
125439	motility	BacteriaNetⓘ	yes	60.20	no
125439	gram_stain	BacteriaNetⓘ	negative	89.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.90	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ochrobactrum cytisi strain LMG 22713
PATRIC: 407152.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	100.00	yes
125438	anaerobic	anaerobicⓘ	no	95.90	yes
125438	spore-forming	spore-formingⓘ	no	91.38	yes
125438	aerobic	aerobicⓘ	yes	86.01	yes
125438	thermophilic	thermophileⓘ	no	98.99	yes
125438	flagellated	motile2+ⓘ	yes	58.89	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020

References

#15442	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19778
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23339	José Luis Zurdo-Pineiro, Raúl Rivas, Martha E. Trujillo, Nieves Vizcaíno, José Antonio Carrasco, Manuel Chamber, Antonio Palomares, Pedro F. Mateos, Eustoquio Martínez-Molina, Encarna Velázquez: Ochrobactrum cytisi sp. nov., isolated from nodules of Cytisus scoparius in Spain. IJSEM 57: 784 - 788 2007 ( DOI 10.1099/ijs.0.64613-0 , PubMed 17392207 )
#37777	; Curators of the CIP;
#60279	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54646
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68369	Automatically annotated from API 20NE .
#68370	Automatically annotated from API 20STR .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118836	Collection of Institut Pasteur ; Curators of the CIP; CIP 109590
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brucella cytisi (DSM 19778, CECT 7172, CIP 109590)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

API 20STR

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_041930165

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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