Name: Brucella pseudintermedia CCUG 55422
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 1891

Type strain:

Species: Brucella pseudintermedia

Culture col. no.: CCUG 55422, DSM 17490, CIP 109116, KCTC 22760, ADV 31

Strain history: 2006, C. Teyssier, Montpellier Univ., Montpellier, France: strain ADV31

NCBI tax ID(s): 370111 (species)

SI-ID 308621

CIP 109116, DSM 17490, VTT E-083043, CCUG 55422

Special Collections

Section

Brucella pseudintermedia CCUG 55422 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from human axillary swab.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Brucellaceae*
	Genus	*Brucella*
	Species	**Brucella pseudintermedia**
	Full Scientific Name (LPSN)	Brucella pseudintermedia (Teyssier et al. 2007) Hördt et al. 2020

Synonym

Ochrobactrum pseudintermedium

Information on morphological and physiological properties Morphology

[Ref.: #23340]	Gram stain	negative

[Ref.: #23340]	Cell length	1.5-1.7 µm

[Ref.: #23340]	Cell width	0.65-0.75 µm

[Ref.: #23340]	Cell shape	rod-shaped

[Ref.: #23340]	Motility	yes

[Ref.: #23340]	Flagellum arrangement	monotrichous, polar

[Ref.: #23340]

Colony color

opaque

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7018]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #7018]

Culture medium growth

[Ref.: #7018]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7018]

Culture medium composition

expand / minimize

[Ref.: #23340]

Culture medium

MacConkey

[Ref.: #23340]

Culture medium growth

[Ref.: #23340]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #23340]

Culture medium growth

[Ref.: #38382]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #38382]

Culture medium growth

[Ref.: #38382]

Culture medium composition

expand / minimize

[Ref.: #7018]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #7018]

Culture medium growth

[Ref.: #7018]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7018]	growth	37 °C
[Ref.: #23340]	growth	25-45 °C
[Ref.: #38382]	growth	37 °C
[Ref.: #60582]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23340]	Oxygen tolerance	aerobe
[Ref.: #60582]	Oxygen tolerance	aerobe

[Ref.: #23340]

Ability of spore formation

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23340]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23340]	adipate ChEBI	-	assimilation
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #23340]	arabinose ChEBI	+	assimilation
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #23340]	cellobiose ChEBI	-	builds acid from
[Ref.: #23340]	coumarate ChEBI	-	builds acid from
[Ref.: #23340]	D-glucose ChEBI	-	builds acid from
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #23340]	D-mannitol ChEBI	-	builds acid from
[Ref.: #23340]	D-mannitol ChEBI	+	assimilation
[Ref.: #68369]	D-mannitol ChEBI	+	assimilation	* from API 20NE
[Ref.: #23340]	D-mannose ChEBI	-	builds acid from
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23340]	D-tagatose ChEBI	+	builds acid from
[Ref.: #23340]	D-trehalose ChEBI	-	builds acid from
[Ref.: #23340]	decanoate ChEBI	+	assimilation
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #23340]	gelatin ChEBI	-	hydrolysis
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23340]	glucose ChEBI	+	assimilation
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23340]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23340]	L-histidine ChEBI	-	assimilation
[Ref.: #23340]	lactate ChEBI	-	assimilation
[Ref.: #23340]	lactate ChEBI	+	builds base from
[Ref.: #23340]	malate ChEBI	+	assimilation
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #23340]	malonate ChEBI	-	builds acid from
[Ref.: #23340]	maltose ChEBI	+	assimilation
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #23340]	mannose ChEBI	+	assimilation
[Ref.: #23340]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #23340]	nitrate ChEBI	+	reduction
[Ref.: #68369]	nitrate ChEBI	+	reduction	* from API 20NE
[Ref.: #23340]	phenylacetate ChEBI	-	assimilation
[Ref.: #23340]	potassium gluconate ChEBI	+	assimilation
[Ref.: #23340]	ribitol ChEBI	+	builds acid from
[Ref.: #23340]	succinate ChEBI	+	builds base from
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23340]	amikacin ChEBI	yes
[Ref.: #23340]	chloramphenicol ChEBI		yes
[Ref.: #23340]	ciprofloxacin ChEBI	yes
[Ref.: #23340]	co-trimoxazole ChEBI	yes
[Ref.: #23340]	colistin ChEBI		yes
[Ref.: #23340]	fosfomycin ChEBI		yes
[Ref.: #23340]	gentamicin ChEBI	yes
[Ref.: #23340]	imipenem ChEBI	yes
[Ref.: #23340]	isepamicin ChEBI	yes
[Ref.: #23340]	nalidixic acid ChEBI	yes
[Ref.: #23340]	netilmycin ChEBI	yes
[Ref.: #23340]	ofloxacin ChEBI	yes
[Ref.: #23340]	pefloxacin ChEBI	yes
[Ref.: #23340]	rifampicin ChEBI	yes
[Ref.: #23340]	tetracycline ChEBI		yes
[Ref.: #23340]	tobramycin ChEBI	yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23340]	acetoin ChEBI	no
[Ref.: #23340]	hydrogen sulfide ChEBI	no
[Ref.: #23340]	indole ChEBI	no
[Ref.: #68369]	indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #23340]		acetoin ChEBI	-
[Ref.: #68369]		indole ChEBI		-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #23340]	alanine arylamidase	-	3.4.11.2
[Ref.: #23340]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23340]	arginine dihydrolase	-	3.5.3.6
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #23340]	beta-galactosidase	-	3.2.1.23
[Ref.: #23340]	beta-glucosidase	-	3.2.1.21
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE
[Ref.: #23340]	beta-glucuronidase	-	3.2.1.31
[Ref.: #23340]	catalase	+	1.11.1.6
[Ref.: #23340]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68369]	cytochrome oxidase	+	1.9.3.1	* from API 20NE
[Ref.: #23340]	DNase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #23340]	leucyl glycin arylamidase	+	3.4.11.1
[Ref.: #23340]	lipase	-
[Ref.: #23340]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23340]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23340]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23340]	phenylalanine deaminase	-	4.3.1.5
[Ref.: #23340]	phosphatase	-
[Ref.: #23340]	proline-arylamidase	+	3.4.11.5
[Ref.: #23340]	pyrrolidonyl arylamidase	+	3.4.19.3
[Ref.: #23340]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23340]	tyrosine arylamidase	+
[Ref.: #23340]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #7018]	26468	+	-	-	-	-	+	-	-	+	+	+	+	+	+	+	+	-	+	-	-	+
[Ref.: #7018]	28416	+	-	-	-	-	+	-	-	-	+	+	+	+	+	+	+	-	+	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7018]	Sample type/isolated from	human axillary swab
[Ref.: #7018]	Geographic location (country and/or sea, region)	Montpellier
[Ref.: #7018]	Country	France
[Ref.: #7018]	Country ISO 3 Code	FRA
[Ref.: #7018]	Continent	Europe

[Ref.: #23340]	Sample type/isolated from	human clinical sample (axillary swab) from a 20 year old man

[Ref.: #60582]	Sample type/isolated from	Human axillary swab,20-yr-old man
[Ref.: #60582]	Sampling date	2002
[Ref.: #60582]	Geographic location (country and/or sea, region)	Montpellier
[Ref.: #60582]	Country	France
[Ref.: #60582]	Country ISO 3 Code	FRA
[Ref.: #60582]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Infection	#Medical environment	#Clinic
#Infection	#Patient	#Swab
#Host	#Human	#Male

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7018]	Pathogenicity (human)	yes, in single cases
[Ref.: #7018]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23340]	Ochrobactrum pseudintermedium ADV31 16S ribosomal RNA gene, partial sequence	DQ365921	1396	GenBank ENA	370111 tax ID
[Ref.: #7018]	Ochrobactrum pseudintermedium ADV43 16S ribosomal RNA gene, partial sequence	DQ365923	1379	GenBank ENA	370111 tax ID

[Ref.: #7018]	GC-content	54.5 mol%
[Ref.: #23340]	GC-content	54.5 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #7018]

Culture collection no.

CCUG 55422, DSM 17490, CIP 109116, KCTC 22760, ADV 31

[Ref.: #71535]

SI-ID 308621

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples.	Teyssier C, Marchandin H, Jean-Pierre H, Masnou A, Dusart G, Jumas-Bilak E	Int J Syst Evol Microbiol	10.1099/ijs.0.64416-0	2007	*

References

#7018

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17490

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23340

Corinne Teyssier, Hélène Marchandin, Hélène Jean-Pierre, Agnès Masnou, Ghislaine Dusart, Estelle Jumas-Bilak: Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples. IJSEM 57: 1007 - 1013 2007 ( DOI 10.1099/ijs.0.64416-0 , PubMed 17473249 )

#38382 ; Curators of the CIP;
#60582 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55422

#68369

Automatically annotated from API 20NE .

#71535

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID308621.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Brucella pseudintermedia in external resources:
SILVA
BRENDA
PANGAEA
GBIF