Brucella pseudintermedia (CCUG 55422, DSM 17490, CIP 109116)

Name: Brucella pseudintermedia (CCUG 55422, DSM 17490, CIP 109116)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1891

Type strain

Culture col. no. CCUG 55422 DSM 17490 CIP 109116 KCTC 22760 ADV 31

NCBI tax ID(s) 370111

Special Collections DSMZ CCUG KCTC CIP Literature strains

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Brucella pseudintermedia CCUG 55422 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from human axillary swab.

Gram-negative motile rod-shaped aerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 308621

CIP 109116,DSM 17490,VTT E-083043,CCUG 55422

@ref 20215

Last LPSN update 2025-12-16 09:47:12

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Brucellaceae

Genus Brucella

Species Brucella pseudintermedia

Full scientific name Brucella pseudintermedia (Teyssier et al. 2007) Hördt et al. 2020

Synonyms (1)

Ochrobactrum pseudintermedium

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
23340	negative	1.5-1.7 µm	0.65-0.75 µm	rod-shaped		monotrichous, polar

Colony morphology

23340

Colony coloropaque

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7018	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
23340	MacConkey
23340	Reasoner's 2A agar (R2A)
38382	MEDIUM 72- for trypto casein soja agar		Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
7018	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
7018	positive	growth	37	mesophilic
23340	positive	growth	25-45
38382	positive	growth	37	mesophilic
60582	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
23340	aerobe
60582	aerobe

Spore formation

23340

Spore formationno

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23340	17426 ChEBI	5-dehydro-D-gluconate	-	builds acid from
23340	17128 ChEBI	adipate	-	assimilation
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
23340	22599 ChEBI	arabinose	+	assimilation
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
23340	17057 ChEBI	cellobiose	-	builds acid from
23340	23399 ChEBI	coumarate	-	builds acid from
23340	17634 ChEBI	D-glucose	-	builds acid from
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
23340	16899 ChEBI	D-mannitol	-	builds acid from
23340	16899 ChEBI	D-mannitol	+	assimilation
68369	16899 ChEBI	D-mannitol	+	assimilation	from API 20NE
23340	16024 ChEBI	D-mannose	-	builds acid from
68369	16024 ChEBI	D-mannose	+	assimilation	from API 20NE
23340	16443 ChEBI	D-tagatose	+	builds acid from
23340	16551 ChEBI	D-trehalose	-	builds acid from
23340	27689 ChEBI	decanoate	+	assimilation
68369	27689 ChEBI	decanoate	+	assimilation	from API 20NE
68369	4853 ChEBI	esculin	+	hydrolysis	from API 20NE
23340	5291 ChEBI	gelatin	-	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	+	assimilation	from API 20NE
23340	17234 ChEBI	glucose	+	assimilation
68369	30849 ChEBI	L-arabinose	+	assimilation	from API 20NE
23340	18403 ChEBI	L-arabitol	-	builds acid from
23340	15971 ChEBI	L-histidine	-	assimilation
23340	24996 ChEBI	lactate	-	assimilation
23340	24996 ChEBI	lactate	+	builds base from
23340	25115 ChEBI	malate	+	assimilation
68369	25115 ChEBI	malate	+	assimilation	from API 20NE
23340	15792 ChEBI	malonate	-	builds acid from
23340	17306 ChEBI	maltose	+	assimilation
68369	17306 ChEBI	maltose	+	assimilation	from API 20NE
23340	37684 ChEBI	mannose	+	assimilation
23340	506227 ChEBI	N-acetylglucosamine	+	assimilation
68369	59640 ChEBI	N-acetylglucosamine	+	assimilation	from API 20NE
23340	17632 ChEBI	nitrate	+	reduction
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
23340	18401 ChEBI	phenylacetate	-	assimilation
23340	32032 ChEBI	potassium gluconate	+	assimilation
23340	15963 ChEBI	ribitol	+	builds acid from
23340	30031 ChEBI	succinate	+	builds base from
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	ChEBI	Metabolite
23340	2637	amikacin
23340	17698	chloramphenicol
23340	100241	ciprofloxacin
23340	3770	co-trimoxazole
23340	37943	colistin
23340	28915	fosfomycin
23340	17833	gentamicin
23340	471744	imipenem
23340	37951	isepamicin
23340	100147	nalidixic acid
23340	7528	netilmycin
23340	7731	ofloxacin
23340	50199	pefloxacin
23340	28077	rifampicin
23340	27902	tetracycline
23340	28864	tobramycin

Metabolite production

@ref	Chebi-ID	Metabolite
23340	15688 ChEBI	acetoin
23340	16136 ChEBI	hydrogen sulfide
23340	35581 ChEBI	indole
68369	35581 ChEBI	indole	from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
23340	15688 ChEBI	acetoin	-
68369	35581 ChEBI	indole		from API 20NE

Enzymes

@ref	Value	Activity	Ec
23340	alanine arylamidase	-	3.4.11.2
23340	alpha-galactosidase	-	3.2.1.22
23340	arginine dihydrolase	-	3.5.3.6
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
23340	beta-galactosidase	-	3.2.1.23
23340	beta-glucosidase	-	3.2.1.21
68369	beta-glucosidase	+	3.2.1.21	from API 20NE
23340	beta-glucuronidase	-	3.2.1.31
23340	catalase	+	1.11.1.6
23340	cytochrome oxidase	+	1.9.3.1
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
23340	DNase	-
68369	gelatinase	-		from API 20NE
23340	leucyl glycin arylamidase	+	3.4.11.1
23340	lipase	-
23340	lysine decarboxylase	-	4.1.1.18
23340	N-acetyl-beta-glucosaminidase	-	3.2.1.52
23340	ornithine decarboxylase	-	4.1.1.17
23340	phenylalanine deaminase	-	4.3.1.5
23340	phosphatase	-
23340	proline-arylamidase	+	3.4.11.5
23340	pyrrolidonyl arylamidase	+	3.4.19.3
23340	tryptophan deaminase	-	4.1.99.1
23340	tyrosine arylamidase	+
23340	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
7018	+	-	-	-	-	+	-	-	+	+	+	+	+	+	+	+	-	+	-	-	+
7018	+	-	-	-	-	+	-	-	-	+	+	+	+	+	+	+	-	+	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Infection	#Medical environment	#Clinic
#Infection	#Patient	#Swab
#Host	#Human	#Male

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
7018	human axillary swab	Montpellier	France	FRA	Europe
23340	human clinical sample (axillary swab) from a 20 year old man
60582	Human axillary swab,20-yr-old man	Montpellier	France	FRA	Europe

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
7018	yes, in single cases	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
23340	Ochrobactrum pseudintermedium ADV31 16S ribosomal RNA gene, partial sequence	DQ365921	1396		370111
7018	Ochrobactrum pseudintermedium ADV43 16S ribosomal RNA gene, partial sequence	DQ365923	1379		370111

GC content

@ref	GC-content (mol%)
7018	54.5
23340	54.5

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Phylogeny	Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples.	Teyssier C, Marchandin H, Jean-Pierre H, Masnou A, Dusart G, Jumas-Bilak E	Int J Syst Evol Microbiol	10.1099/ijs.0.64416-0	2007

References

#7018	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17490
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23340	Corinne Teyssier, Hélène Marchandin, Hélène Jean-Pierre, Agnès Masnou, Ghislaine Dusart, Estelle Jumas-Bilak: Ochrobactrum pseudintermedium sp. nov., a novel member of the family Brucellaceae, isolated from human clinical samples. IJSEM 57: 1007 - 1013 2007 ( DOI 10.1099/ijs.0.64416-0 , PubMed 17473249 )
#38382	; Curators of the CIP;
#60582	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55422
#68369	Automatically annotated from API 20NE .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brucella pseudintermedia (CCUG 55422, DSM 17490, CIP 109116)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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