Brevibacterium linens (DSM 20158, ATCC 19391, IAM 13190)

Name: Brevibacterium linens (DSM 20158, ATCC 19391, IAM 13190)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1841

Type strain

Culture col. no. DSM 20158 ATCC 19391 IAM 13190 NCIB 11438 KY 3469 8 more

NCBI tax ID(s) 1703

Special Collections DSMZ JCM KCTC

History <- ATCC <- Kyowa Ferm. Ind. Co., Ltd., KY 3469 DSM 20158 <-- ATCC 19391 <-- Kyowa Hakko Kogyo Co., Ltd.; KY 3469.

Links

Brevibacterium linens DSM 20158 is a bacterium of the family Brevibacteriaceae.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 36968

ATCC 19391,CCM 47,NCIB 11438,IAM 13190,JCM 6200,CCRC 10041,IMET 10776,NCIMB 11438,CECT 75,KCTC 1843,BCRC 10041,IAM 12347,DSM 20158

@ref 20215

Last LPSN update 2026-02-09 11:18:11

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Brevibacteriaceae

Genus Brevibacterium

Species Brevibacterium linens

Full scientific name Brevibacterium linens (Wolff 1910) Breed 1953 (Approved Lists 1980)

Synonyms (1)

"Bacterium linens"

BacDive ID	Other strains from **Brevibacterium linens** (6)	Type strain
1842	B. linens 56b, 56-B, DSM 20425, ATCC 9172, NCIB 9909, ... (type strain)
135980	B. linens 1045-85, CIP 102069
142385	B. linens CCUG 12168
144230	B. linens CCUG 23846, CCM 2782
144240	B. linens CCUG 23896, LMG 3915
161336	B. linens JCM 1328, ATCC 8377, BCRC 11884, IAM 12436, ...

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	positive	91.817
125439	positive	90.1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8582	CORYNEBACTERIUM AGAR (DSMZ Medium 53)		Medium recipe at MediaDive	Name: CORYNEBACTERIUM AGAR (DSMZ Medium 53) Composition: Agar 15.0 g/l Casein peptone 10.0 g/l NaCl 5.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
8582	positive	growth	30
67770	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	90.492

Spore formation

@ref	Spore formation	Confidence
125439		94

Compound production

@ref	Compound
8582	L lysine
8582	lytic enzymes
20216	L-Lysin

Murein

@ref	Murein short key	Type
8582	A31	A1gamma m-Dpm-direct

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	adipate degradation	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	acetate fermentation	100	4 of 4
66794	phenylmercury acetate degradation	100	2 of 2
66794	allantoin degradation	100	9 of 9
66794	ethylmalonyl-CoA pathway	100	5 of 5
66794	lipoate biosynthesis	100	5 of 5
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	propanol degradation	100	7 of 7
66794	valine metabolism	100	9 of 9
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	ethanol fermentation	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	gallate degradation	100	5 of 5
66794	glycolate and glyoxylate degradation	100	6 of 6
66794	reductive acetyl coenzyme A pathway	100	7 of 7
66794	metabolism of amino sugars and derivatives	100	5 of 5
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	methylglyoxal degradation	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	cyanate degradation	100	3 of 3
66794	biotin biosynthesis	100	4 of 4
66794	teichoic acid biosynthesis	100	1 of 1
66794	denitrification	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	peptidoglycan biosynthesis	93.33	14 of 15
66794	phenylalanine metabolism	92.31	12 of 13
66794	vitamin B1 metabolism	92.31	12 of 13
66794	leucine metabolism	92.31	12 of 13
66794	threonine metabolism	90	9 of 10
66794	glutamate and glutamine metabolism	89.29	25 of 28
66794	chorismate metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	NAD metabolism	88.89	16 of 18
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	isoleucine metabolism	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	citric acid cycle	85.71	12 of 14
66794	phenol degradation	85	17 of 20
66794	urea cycle	84.62	11 of 13
66794	proline metabolism	81.82	9 of 11
66794	creatinine degradation	80	4 of 5
66794	3-chlorocatechol degradation	80	4 of 5
66794	Entner Doudoroff pathway	80	8 of 10
66794	purine metabolism	79.79	75 of 94
66794	photosynthesis	78.57	11 of 14
66794	heme metabolism	78.57	11 of 14
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	glycolysis	76.47	13 of 17
66794	alanine metabolism	75.86	22 of 29
66794	histidine metabolism	75.86	22 of 29
66794	pyrimidine metabolism	75.56	34 of 45
66794	lactate fermentation	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	ketogluconate metabolism	75	6 of 8
66794	gluconeogenesis	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	tryptophan metabolism	73.68	28 of 38
66794	vitamin B6 metabolism	72.73	8 of 11
66794	pentose phosphate pathway	72.73	8 of 11
66794	cysteine metabolism	72.22	13 of 18
66794	tetrahydrofolate metabolism	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	arginine metabolism	70.83	17 of 24
66794	propionate fermentation	70	7 of 10
66794	methionine metabolism	69.23	18 of 26
66794	methane metabolism	66.67	2 of 3
66794	enterobactin biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	d-mannose degradation	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	oxidative phosphorylation	64.84	59 of 91
66794	tyrosine metabolism	64.29	9 of 14
66794	lysine metabolism	64.29	27 of 42
66794	carotenoid biosynthesis	63.64	14 of 22
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	non-pathway related	60.53	23 of 38
66794	glycine betaine biosynthesis	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	degradation of aromatic, nitrogen containing compounds	58.33	7 of 12
66794	lipid metabolism	58.06	18 of 31
66794	glutathione metabolism	57.14	8 of 14
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	nitrate assimilation	55.56	5 of 9
66794	degradation of hexoses	55.56	10 of 18
66794	metabolism of disaccharids	54.55	6 of 11
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	toluene degradation	50	2 of 4
66794	butanoate fermentation	50	2 of 4
66794	pantothenate biosynthesis	50	3 of 6
66794	cyclohexanol degradation	50	2 of 4
66794	kanosamine biosynthesis II	50	1 of 2
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	myo-inositol biosynthesis	50	5 of 10
66794	glycine metabolism	50	5 of 10
66794	quinate degradation	50	1 of 2
66794	resorcinol degradation	50	1 of 2
66794	sulfate reduction	46.15	6 of 13
66794	degradation of sugar acids	44	11 of 25
66794	degradation of sugar alcohols	43.75	7 of 16
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	degradation of pentoses	42.86	12 of 28
66794	mevalonate metabolism	42.86	3 of 7
66794	bile acid biosynthesis, neutral pathway	41.18	7 of 17
66794	cellulose degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	glycogen metabolism	40	2 of 5
66794	chlorophyll metabolism	38.89	7 of 18
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	carnitine metabolism	37.5	3 of 8
66794	d-xylose degradation	36.36	4 of 11
66794	polyamine pathway	34.78	8 of 23
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	sulfoquinovose degradation	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	starch degradation	30	3 of 10
66794	arachidonic acid metabolism	27.78	5 of 18
66794	ascorbate metabolism	27.27	6 of 22
66794	cholesterol biosynthesis	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	catecholamine biosynthesis	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	daunorubicin biosynthesis	22.22	2 of 9

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8582	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM399933v1 assembly for Brevibacterium linens ATCC 19391	complete	GCA_003999335	1703.50	2843156836	1703	95.83

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Brevibacterium linens strain DSM 20158 16S-23S ribosomal intergenic spacer sequence	U59265	404		1703

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Brevibacterium linens strain ATCC 19391
PATRIC: 1703.50

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.00	no
125439	motility	BacteriaNetⓘ	no	50.10	no
125439	gram_stain	BacteriaNetⓘ	positive	90.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	83.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Brevibacterium linens ATCC 19391
NCBI: GCA_003999335.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.82	no
125438	anaerobic	anaerobicⓘ	no	96.94	no
125438	aerobic	aerobicⓘ	yes	90.49	no
125438	spore-forming	spore-formingⓘ	no	76.81	no
125438	thermophilic	thermophileⓘ	no	96.50	no
125438	flagellated	motile2+ⓘ	no	89.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Optimized production, purification and characterization of alpha amylase by Brevibacterium linens DSM 20158, using bio-statistical approach	Shabbiri K, `Adnan A, Noor B, Jamil S.	Ann Microbiol	10.1007/s13213-011-0286-6	2012
	Phylogenetic analysis of the coryneform bacteria by 5S rRNA sequences.	Park YH, Hori H, Suzuki K, Osawa S, Komagata K.	J Bacteriol	10.1128/jb.169.5.1801-1806.1987	1987
Phylogeny	Differentiation of Brevibacterium spp. encountered in clinical specimens.	Funke G, Carlotti A.	J Clin Microbiol	10.1128/jcm.32.7.1729-1732.1994	1994
	High-frequency conjugal plasmid transfer from gram-negative Escherichia coli to various gram-positive coryneform bacteria.	Schafer A, Kalinowski J, Simon R, Seep-Feldhaus AH, Puhler A.	J Bacteriol	10.1128/jb.172.3.1663-1666.1990	1990
Genetics	Draft genome sequence of Brevibacterium linens MA5, an isolate from Danbo cheese that carries the bacteriocin gene cluster.	Zhao S, Tadesse BT, Gu L, Solem C.	Microbiol Resour Announc	10.1128/mra.00963-25	2025
Genetics	Mobilome of Brevibacterium aurantiacum Sheds Light on Its Genetic Diversity and Its Adaptation to Smear-Ripened Cheeses.	Levesque S, de Melo AG, Labrie SJ, Moineau S.	Front Microbiol	10.3389/fmicb.2019.01270	2019
Genetics	Identification, characterization, and genome sequencing of Brevibacterium sediminis MG-1 isolate with growth-promoting properties.	Lutfullin MT, Lutfullina GF, Pudova DS, Akosah YA, Shagimardanova EI, Vologin SG, Sharipova MR, Mardanova AM.	3 Biotech	10.1007/s13205-022-03392-z	2022
Phylogeny	Comprehensive analysis of genomic variation, pan-genome and biosynthetic potential of Corynebacterium glutamicum strains.	Rahman MS, Shimul MEK, Parvez MAK.	PLoS One	10.1371/journal.pone.0299588	2024
	Designing primers and evaluation of the efficiency of propidium monoazide - Quantitative polymerase chain reaction for counting the viable cells of Lactobacillus gasseri and Lactobacillus salivarius.	Lai CH, Wu SR, Pang JC, Ramireddy L, Chiang YC, Lin CK, Tsen HY.	J Food Drug Anal	10.1016/j.jfda.2016.10.004	2017
Enzymology	Human infections caused by Brevibacterium casei, formerly CDC groups B-1 and B-3.	Gruner E, Steigerwalt AG, Hollis DG, Weyant RS, Weaver RE, Moss CW, Daneshvar M, Brown JM, Brenner DJ.	J Clin Microbiol	10.1128/jcm.32.6.1511-1518.1994	1994
Enzymology	Distribution and persistence of Staphylococcus and Micrococcus species and other aerobic bacteria on human skin.	Kloos WE, Musselwhite MS.	Appl Microbiol	10.1128/am.30.3.381-395.1975	1975
Enzymology	An investigation into membrane bound redox carriers involved in energy transduction mechanism in Brevibacterium linens DSM 20158 with unsequenced genome.	Shabbiri K, Botting CH, Adnan A, Fuszard M, Naseem S, Ahmed S, Shujaat S, Syed Q, Ahmad W	J Membr Biol	10.1007/s00232-014-9641-4	2014
Metabolism	Charting the cellular and extracellular proteome analysis of Brevibacterium linens DSM 20158 with unsequenced genome by mass spectrometry-driven sequence similarity searches.	Shabbiri K, Botting CH, Adnan A, Fuszard M	J Proteomics	10.1016/j.jprot.2013.02.029	2013
Biotechnology	Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach.	Shabbiri K, Adnan A, Jamil S, Ahmad W, Noor B, Rafique HM	Braz J Microbiol	10.1590/S1517-838220120003000031	2012
Metabolism	Carnitine acts as a compatible solute in Brevibacterium linens.	Jebbar M, Champion C, Blanco C, Bonnassie S	Res Microbiol	10.1016/s0923-2508(98)80081-8	1998

References

#8582	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20158
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI):
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Brevibacterium linens (DSM 20158, ATCC 19391, IAM 13190)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Murein

Pathway annotation

Isolation, sampling and environmental information

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003999335

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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