Name: Thermoanaerobacterium thermosaccharolyticum McClung 2032
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 18044

Type strain:

Species: Thermoanaerobacterium thermosaccharolyticum

Strain Designation: McClung 2032, 2032

Culture col. no.: DSM 571, ATCC 7956, NCA 3814, NCIMB 9385, WDCM 00135

Strain history: <- F. Hollaus, Zuckerforschungs-Institut, Fuchsenbigl, Haringsee, Austria <- NCIB <- ATCC <- National Canner's Association (NCA), Washington, D.C., USA; McClung 2032

NCBI tax ID(s): 580327 (strain), 1517 (species)

SI-ID 1979

LMG 2811, ATCC 7956, CECT 487, NCIB 9385, NCIMB 9385, CCRC 10653, CCRC 14425, CECT 5852, HAMBI 2225, BCRC 14425, VTT E-032438, DSM 571

Other strains from Thermoanaerobacterium thermosaccharolyticum

T. thermosaccharolyticum T9-1, DSM 572, ATCC 27384
T. thermosaccharolyticum T9-3R, DSM 573
T. thermosaccharolyticum 1917, Prévot, 1917, Prévot, DSM ...
T. thermosaccharolyticum Haren, DSM 7416

Section

Thermoanaerobacterium thermosaccharolyticum McClung 2032 is an anaerobe, spore-forming, thermophilic bacterium of the family Thermoanaerobacteraceae.

Gram-positive
spore-forming
anaerobe
thermophilic
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Thermoanaerobacterales*
	Family	*Thermoanaerobacteraceae*
	Genus	*Thermoanaerobacterium*
	Species	**Thermoanaerobacterium thermosaccharolyticum**
	Full Scientific Name (LPSN)	Thermoanaerobacterium thermosaccharolyticum (McClung 1935) Collins et al. 1994

Synonym

Clostridium thermosaccharolyticum

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

Information on culture and growth conditions Culture and growth conditions

[Ref.: #288]

Culture medium

THERMOANAEROBACTER MEDIUM (DSMZ Medium 61)

[Ref.: #288]

Culture medium growth

[Ref.: #288]

Culture medium link

Medium recipe at MediaDive

[Ref.: #288]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #288]			growth	60 °C

[Ref.: #288]	Temperature range	thermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %99.999

Information on physiology and metabolism Physiology and metabolism

[Ref.: #288]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.573

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	cellulose degradation	100	5 of 5
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	propanol degradation	100	7 of 7
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	vitamin B1 metabolism	92.31	12 of 13
[Ref.: #66794]	threonine metabolism	90	9 of 10
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	aspartate and asparagine metabolism	88.89	8 of 9
[Ref.: #66794]	isoleucine metabolism	87.5	7 of 8
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	lipoate biosynthesis	80	4 of 5
[Ref.: #66794]	starch degradation	80	8 of 10
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	vitamin B12 metabolism	76.47	26 of 34
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	sulfopterin metabolism	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	75	6 of 8
[Ref.: #66794]	pyrimidine metabolism	73.33	33 of 45
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	pentose phosphate pathway	72.73	8 of 11
[Ref.: #66794]	metabolism of disaccharids	72.73	8 of 11
[Ref.: #66794]	alanine metabolism	72.41	21 of 29
[Ref.: #66794]	glutamate and glutamine metabolism	71.43	20 of 28
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	ubiquinone biosynthesis	71.43	5 of 7
[Ref.: #66794]	purine metabolism	71.28	67 of 94
[Ref.: #66794]	Entner Doudoroff pathway	70	7 of 10
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	methionine metabolism	69.23	18 of 26
[Ref.: #66794]	degradation of sugar alcohols	68.75	11 of 16
[Ref.: #66794]	CO2 fixation in Crenarchaeota	66.67	6 of 9
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	66.67	4 of 6
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	selenocysteine biosynthesis	66.67	4 of 6
[Ref.: #66794]	methane metabolism	66.67	2 of 3
[Ref.: #66794]	histidine metabolism	65.52	19 of 29
[Ref.: #66794]	isoprenoid biosynthesis	65.38	17 of 26
[Ref.: #66794]	glycolysis	64.71	11 of 17
[Ref.: #66794]	tetrahydrofolate metabolism	64.29	9 of 14
[Ref.: #66794]	citric acid cycle	64.29	9 of 14
[Ref.: #66794]	glutathione metabolism	64.29	9 of 14
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	d-xylose degradation	63.64	7 of 11
[Ref.: #66794]	arginine metabolism	62.5	15 of 24
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	oxidative phosphorylation	60.44	55 of 91
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	factor 420 biosynthesis	60	3 of 5
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	hydrogen production	60	3 of 5
[Ref.: #66794]	degradation of pentoses	57.14	16 of 28
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	tryptophan metabolism	55.26	21 of 38
[Ref.: #66794]	non-pathway related	52.63	20 of 38
[Ref.: #66794]	degradation of sugar acids	52	13 of 25
[Ref.: #66794]	lipid metabolism	51.61	16 of 31
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	aminopropanol phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	tyrosine metabolism	50	7 of 14
[Ref.: #66794]	phenylmercury acetate degradation	50	1 of 2
[Ref.: #66794]	cysteine metabolism	50	9 of 18
[Ref.: #66794]	biotin biosynthesis	50	2 of 4
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	lactate fermentation	50	2 of 4
[Ref.: #66794]	sulfate reduction	46.15	6 of 13
[Ref.: #66794]	leucine metabolism	46.15	6 of 13
[Ref.: #66794]	urea cycle	46.15	6 of 13
[Ref.: #66794]	proline metabolism	45.45	5 of 11
[Ref.: #66794]	lysine metabolism	45.24	19 of 42
[Ref.: #66794]	nitrate assimilation	44.44	4 of 9
[Ref.: #66794]	molybdenum cofactor biosynthesis	44.44	4 of 9
[Ref.: #66794]	degradation of hexoses	44.44	8 of 18
[Ref.: #66794]	reductive acetyl coenzyme A pathway	42.86	3 of 7
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	polyamine pathway	34.78	8 of 23
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	30.77	4 of 13
[Ref.: #66794]	coenzyme M biosynthesis	30	3 of 10
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	benzoyl-CoA degradation	28.57	2 of 7
[Ref.: #66794]	vitamin B6 metabolism	27.27	3 of 11
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	phenylpropanoid biosynthesis	23.08	3 of 13
		Only first 10 entries are displayed. Click here to see all.

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #288]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermoanaerobacterium thermosaccharolyticum DSM 571	GCA_000145615	complete	GenBank	580327 tax ID	*
[Ref.: #66792]	Thermoanaerobacterium thermosaccharolyticum DSM 571	580327.4	complete	PATRIC	580327 tax ID	*
[Ref.: #66792]	Thermoanaerobacterium thermosaccharolyticum DSM 571	648028060	complete	JGI IMG/M	580327 tax ID	*

[Ref.: #288]	GC-content	34 mol%	sequence analysis
[Ref.: #288]	GC-content	29.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermoanaerobacterium thermosaccharolyticum DSM 571 PATRIC: 580327.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermoanaerobacterium thermosaccharolyticum DSM 571 DSM 571 NCBI: GCA_000145615.1
[Ref.: #69480]	motile	yes	86.296	no
[Ref.: #69480]	flagellated	no	83.177	no
[Ref.: #69480]	gram-positive	no	74.237	no
[Ref.: #69480]	anaerobic	yes	99.484	yes
[Ref.: #69480]	aerobic	no	98.83	yes
[Ref.: #69480]	halophile	no	96.855	no
[Ref.: #69480]	spore-forming	yes	95.141	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	90.528	no
[Ref.: #69480]	glucose-ferment	yes	63.563	no

Availability in culture collections External links

[Ref.: #288]

Culture collection no.

DSM 571, ATCC 7956, NCA 3814, NCIMB 9385, WDCM 00135

[Ref.: #87033]

SI-ID 1979

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Stress	Effect of pH on Thermoanaerobacterium thermosaccharolyticum DSM 571 growth, spore heat resistance and recovery.	Mtimet N, Guegan S, Durand L, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O	Food Microbiol	10.1016/j.fm.2015.11.015	2015	*
Metabolism	Driving carbon flux through exogenous butyryl-CoA: Acetate CoA-transferase to produce butyric acid at high titer in Thermobifida fusca.	Deng Y, Mao Y, Zhang X	J Biotechnol	10.1016/j.jbiotec.2015.10.022	2015	*
Metabolism	Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum beta-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose.	Zhao L, Pang Q, Xie J, Pei J, Wang F, Fan S	BMC Biotechnol	10.1186/1472-6750-13-101	2013	*
Metabolism	[Interrelation between the pathways of isoprenoid biosynthesis and carbon source catabolism in anaerobic and facultatively anaerobic bacteria].	Trutko SM, Shcherbakova VA, Ivanova IV, Lysanskaia VIa, Arkhipova OV, Chuvil'skaia NA, Baskunov BP, Ostrovskii DN, Akimenko VK	Mikrobiologiia		2008	*
Enzymology	Purification and properties of an amylopullulanase, a glucoamylase, and an alpha-glucosidase in the amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum.	Ganghofner D, Kellermann J, Staudenbauer WL, Bronnenmeier K	Biosci Biotechnol Biochem	10.1271/bbb.62.302	1998	*
Enzymology	Characterization of two novel thermostable esterases from Thermoanaerobacterium thermosaccharolyticum.	Li W, Shi H, Ding H, Wang L, Zhang Y, Li X, Wang F	Protein Expr Purif	10.1016/j.pep.2018.04.010	2018	*
Enzymology	Characterization of Glycerol Dehydrogenase from Thermoanaerobacterium thermosaccharolyticum DSM 571 and GGG Motif Identification.	Wang L, Wang J, Shi H, Gu H, Zhang Y, Li X, Wang F	J Microbiol Biotechnol	10.4014/jmb.1512.12051	2016	*
Enzymology	Production, purification, and characterization of a cellulase-free thermostable endo-xylanase from Thermoanaerobacterium thermosaccharolyticum DSM 571.	Li X, Shi H, Ding H, Zhang Y, Wang F	Appl Biochem Biotechnol	10.1007/s12010-014-1135-4	2014	*
Enzymology	Characterization of a novel thermostable chitin-binding domain and its application in immobilization of a multifunctional hemicellulase.	Wang Q, Xue Y, Wu X	J Agric Food Chem	10.1021/jf3041275	2013	*
Enzymology	Thermoanaerobacterium thermosaccharolyticum beta-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose.	Pei J, Pang Q, Zhao L, Fan S, Shi H	Biotechnol Biofuels	10.1186/1754-6834-5-31	2012	*
Enzymology	Kinetics of growth and metabolism of Clostridium thermosaccharolyticum culture. Isolation and characteristics of its plasmids.	Belogurova NG, Mosolova TP, Kalyuzhnyy SV, Varfolomeyev SD	Appl Biochem Biotechnol	10.1007/BF02921510	1991	*
Enzymology	Cloning, expression and characterization of a glycoside hydrolase family 51 alpha-l-arabinofuranosidase from Thermoanaerobacterium thermosaccharolyticum DSM 571.	Shi H, Gao F, Yan X, Li Q, Nie X	3 Biotech	10.1007/s13205-022-03254-8	2022	*

References

#288	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 571

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#87033

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1979.1 )

* These data were automatically processed and therefore are not curated

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Thermoanaerobacterium thermosaccharolyticum DSM 571

Thermoanaerobacterium thermosaccharolyticum DSM 571 DSM 571

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