Strain identifier
BacDive ID: 18044
Type strain: 
Species: Thermoanaerobacterium thermosaccharolyticum
Strain Designation: McClung 2032, 2032
Strain history: <- F. Hollaus, Zuckerforschungs-Institut, Fuchsenbigl, Haringsee, Austria <- NCIB <- ATCC <- National Canner's Association (NCA), Washington, D.C., USA; McClung 2032
NCBI tax ID(s): 580327 (strain), 1517 (species)
version 8.1 (current version)
General
@ref: 288
BacDive-ID: 18044
DSM-Number: 571
keywords: genome sequence, Bacteria, anaerobe, spore-forming, thermophilic, Gram-positive
description: Thermoanaerobacterium thermosaccharolyticum McClung 2032 is an anaerobe, spore-forming, thermophilic bacterium of the family Thermoanaerobacteraceae.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1517 | species |
| 580327 | strain |
strain history
- @ref: 288
- history: <- F. Hollaus, Zuckerforschungs-Institut, Fuchsenbigl, Haringsee, Austria <- NCIB <- ATCC <- National Canner's Association (NCA), Washington, D.C., USA; McClung 2032
doi: 10.13145/bacdive18044.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Thermoanaerobacterium
- species: Thermoanaerobacterium thermosaccharolyticum
- full scientific name: Thermoanaerobacterium thermosaccharolyticum (McClung 1935) Collins et al. 1994
synonyms
- @ref: 20215
- synonym: Clostridium thermosaccharolyticum
@ref: 288
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Thermoanaerobacterium
species: Thermoanaerobacterium thermosaccharolyticum
full scientific name: Thermoanaerobacterium thermosaccharolyticum (McClung 1935) Collins et al. 1994
strain designation: McClung 2032, 2032
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 100
Culture and growth conditions
culture medium
- @ref: 288
- name: THERMOANAEROBACTER MEDIUM (DSMZ Medium 61)
- growth: yes
- link: https://mediadive.dsmz.de/medium/61
- composition: Name: THERMOANAEROBACTER MEDIUM (DSMZ Medium 61) Composition: Sucrose 10.0 g/l Tryptone 10.0 g/l Yeast extract 2.0 g/l Na2SO3 0.2 g/l FeSO4 x 7 H2O 0.2 g/l Na2S2O3 x 5 H2O 0.08 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 288 | positive | growth | 60 | thermophilic | |
| 69480 | thermophilic | 99.999 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 288 | anaerobe | |
| 69480 | anaerobe | 100 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 99.573 |
Safety information
risk assessment
- @ref: 288
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Thermoanaerobacterium thermosaccharolyticum DSM 571 | GCA_000145615 | complete | ncbi | 580327 |
| 66792 | Thermoanaerobacterium thermosaccharolyticum DSM 571 | 580327.4 | complete | patric | 580327 |
| 66792 | Thermoanaerobacterium thermosaccharolyticum DSM 571 | 648028060 | complete | img | 580327 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 288 | 34 | sequence analysis |
| 288 | 29.0 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 86.296 | no |
| flagellated | no | 83.177 | no |
| gram-positive | no | 74.237 | no |
| anaerobic | yes | 99.484 | yes |
| aerobic | no | 98.83 | yes |
| halophile | no | 96.855 | no |
| spore-forming | yes | 95.141 | no |
| thermophile | yes | 100 | yes |
| glucose-util | yes | 90.528 | no |
| glucose-ferment | yes | 63.563 | no |
External links
@ref: 288
culture collection no.: DSM 571, ATCC 7956, NCA 3814, NCIMB 9385, WDCM 00135
straininfo link
- @ref: 87033
- straininfo: 1979
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Enzymology | 2024977 | Kinetics of growth and metabolism of Clostridium thermosaccharolyticum culture. Isolation and characteristics of its plasmids. | Belogurova NG, Mosolova TP, Kalyuzhnyy SV, Varfolomeyev SD | Appl Biochem Biotechnol | 10.1007/BF02921510 | 1991 | Anti-Bacterial Agents/pharmacology, Clostridium/genetics/*growth & development/metabolism, DNA, Bacterial/genetics, Glucose/metabolism, Hydrogen-Ion Concentration, Kinetics, Plasmids, Restriction Mapping | Metabolism |
| Enzymology | 9532787 | Purification and properties of an amylopullulanase, a glucoamylase, and an alpha-glucosidase in the amylolytic enzyme system of Thermoanaerobacterium thermosaccharolyticum. | Ganghofner D, Kellermann J, Staudenbauer WL, Bronnenmeier K | Biosci Biotechnol Biochem | 10.1271/bbb.62.302 | 1998 | Amino Acid Sequence, Chemical Fractionation, Chromatography, Agarose, Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Clostridium/*enzymology, Electrophoresis, Polyacrylamide Gel, Glucan 1,4-alpha-Glucosidase/chemistry/*isolation & purification/metabolism, Glucans/metabolism, Glucose/metabolism, Glycoside Hydrolases/chemistry/*isolation & purification/metabolism, Hydrogen-Ion Concentration, Hydrolysis, Isoelectric Focusing, Molecular Sequence Data, Molecular Weight, Sequence Alignment, Starch/metabolism, Substrate Specificity, Temperature, Ultrafiltration, alpha-Glucosidases/chemistry/*isolation & purification/metabolism | Metabolism |
| Metabolism | 18683645 | [Interrelation between the pathways of isoprenoid biosynthesis and carbon source catabolism in anaerobic and facultatively anaerobic bacteria]. | Trutko SM, Shcherbakova VA, Ivanova IV, Lysanskaia VIa, Arkhipova OV, Chuvil'skaia NA, Baskunov BP, Ostrovskii DN, Akimenko VK | Mikrobiologiia | 2008 | Bacteria, Anaerobic/growth & development/*metabolism, Carbon/metabolism, Clostridium/metabolism, Glucose/*metabolism, Lactobacillus/metabolism, Mevalonic Acid/metabolism, Pentose Phosphate Pathway, Substrate Specificity, Terpenes/*metabolism, Xylose/*metabolism | ||
| Enzymology | 22571470 | Thermoanaerobacterium thermosaccharolyticum beta-glucosidase: a glucose-tolerant enzyme with high specific activity for cellobiose. | Pei J, Pang Q, Zhao L, Fan S, Shi H | Biotechnol Biofuels | 10.1186/1754-6834-5-31 | 2012 | ||
| Enzymology | 23470102 | Characterization of a novel thermostable chitin-binding domain and its application in immobilization of a multifunctional hemicellulase. | Wang Q, Xue Y, Wu X | J Agric Food Chem | 10.1021/jf3041275 | 2013 | Amino Acid Sequence, Binding Sites, Chitin/*metabolism, Cloning, Molecular, Enzyme Stability, Enzymes, Immobilized/*chemistry, Glycoside Hydrolases/*chemistry/genetics/*metabolism, *Hot Temperature, Hydrogen-Ion Concentration, Molecular Sequence Data, Multifunctional Enzymes/chemistry/metabolism, Recombinant Fusion Proteins/chemistry/genetics/metabolism, Thermoanaerobacterium/enzymology, Xylose/biosynthesis | Metabolism |
| Metabolism | 24228818 | Enzymatic properties of Thermoanaerobacterium thermosaccharolyticum beta-glucosidase fused to Clostridium cellulovorans cellulose binding domain and its application in hydrolysis of microcrystalline cellulose. | Zhao L, Pang Q, Xie J, Pei J, Wang F, Fan S | BMC Biotechnol | 10.1186/1472-6750-13-101 | 2013 | Cellulase/*metabolism, Cellulose/*metabolism, Cloning, Molecular, Clostridium cellulovorans/*genetics/metabolism, DNA, Bacterial/genetics, Escherichia coli/genetics, Hydrolysis, Multienzyme Complexes/*metabolism, Plasmids/genetics, Protein Binding, Recombinant Proteins/genetics/metabolism, Thermoanaerobacterium/*enzymology, beta-Glucosidase/*metabolism | Enzymology |
| Enzymology | 25261357 | Production, purification, and characterization of a cellulase-free thermostable endo-xylanase from Thermoanaerobacterium thermosaccharolyticum DSM 571. | Li X, Shi H, Ding H, Zhang Y, Wang F | Appl Biochem Biotechnol | 10.1007/s12010-014-1135-4 | 2014 | Cloning, Molecular, *Endo-1,4-beta Xylanases/biosynthesis/chemistry/genetics/isolation & purification, *Gene Expression, Hydrogen-Ion Concentration, Metals/chemistry, Recombinant Proteins/biosynthesis/classification/genetics/isolation & purification, Substrate Specificity, Thermoanaerobacterium/*enzymology/genetics, Xylans/*chemistry | Phylogeny |
| Metabolism | 26535965 | Driving carbon flux through exogenous butyryl-CoA: Acetate CoA-transferase to produce butyric acid at high titer in Thermobifida fusca. | Deng Y, Mao Y, Zhang X | J Biotechnol | 10.1016/j.jbiotec.2015.10.022 | 2015 | Actinomycetales/drug effects/*metabolism, Acyl Coenzyme A/*metabolism, Aerobiosis/drug effects, Batch Cell Culture Techniques, Bioreactors/microbiology, Butyric Acid/*metabolism, Carbon/*metabolism, Cellulose/pharmacology, Chromosomes, Bacterial/genetics, Coenzyme A-Transferases/genetics/*metabolism, Fermentation/drug effects, Genetic Engineering, Mutagenesis, Insertional/genetics, Waste Products, Zea mays/chemistry | Pathogenicity |
| Stress | 26742617 | Effect of pH on Thermoanaerobacterium thermosaccharolyticum DSM 571 growth, spore heat resistance and recovery. | Mtimet N, Guegan S, Durand L, Mathot AG, Venaille L, Leguerinel I, Coroller L, Couvert O | Food Microbiol | 10.1016/j.fm.2015.11.015 | 2015 | Colony Count, Microbial, Hot Temperature, Hydrogen-Ion Concentration, Microbial Viability, Spores, Bacterial/chemistry/*growth & development, Thermoanaerobacterium/chemistry/*growth & development | |
| Enzymology | 26975764 | Characterization of Glycerol Dehydrogenase from Thermoanaerobacterium thermosaccharolyticum DSM 571 and GGG Motif Identification. | Wang L, Wang J, Shi H, Gu H, Zhang Y, Li X, Wang F | J Microbiol Biotechnol | 10.4014/jmb.1512.12051 | 2016 | Amino Acid Motifs, Catalysis, Cloning, Molecular, Escherichia coli/genetics, Glycerol/metabolism, Hydrogen-Ion Concentration, Kinetics, Manganese/metabolism, Models, Molecular, Mutagenesis, Site-Directed, Oxidation-Reduction, Phylogeny, Recombinant Proteins/chemistry/metabolism, Sequence Alignment, Sugar Alcohol Dehydrogenases/*chemistry/genetics/*metabolism, Thermoanaerobacterium/*enzymology | Metabolism |
| Enzymology | 29684442 | Characterization of two novel thermostable esterases from Thermoanaerobacterium thermosaccharolyticum. | Li W, Shi H, Ding H, Wang L, Zhang Y, Li X, Wang F | Protein Expr Purif | 10.1016/j.pep.2018.04.010 | 2018 | Acyclic Monoterpenes, Bacterial Proteins/genetics/isolation & purification/*metabolism, Caprylates/metabolism, Chromatography, Affinity, Cloning, Molecular, Enzyme Assays, Enzyme Stability, Escherichia coli/enzymology/*genetics, Esterases/genetics/isolation & purification/*metabolism, Gene Expression, Genetic Vectors/chemistry/metabolism, Hot Temperature, Hydrogen-Ion Concentration, Hydrolysis, Isoenzymes/genetics/isolation & purification/metabolism, Kinetics, Monoterpenes/*metabolism, Recombinant Proteins/genetics/isolation & purification/metabolism, Substrate Specificity, Thermoanaerobacterium/chemistry/*enzymology | Metabolism |
| Enzymology | 35855476 | Cloning, expression and characterization of a glycoside hydrolase family 51 alpha-l-arabinofuranosidase from Thermoanaerobacterium thermosaccharolyticum DSM 571. | Shi H, Gao F, Yan X, Li Q, Nie X | 3 Biotech | 10.1007/s13205-022-03254-8 | 2022 |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 288 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 571) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-571 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 87033 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID1979.1 | StrainInfo: A central database for resolving microbial strain identifiers |