Peptoniphilus asaccharolyticus (DSM 20463, ATCC 14963, NCIB 10074)

Name: Peptoniphilus asaccharolyticus (DSM 20463, ATCC 14963, NCIB 10074)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17996

Type strain

Strain Designation BAI, UW 228

Culture col. no. DSM 20463 ATCC 14963 NCIB 10074 CCUG 9988 BAI 7 more

NCBI tax ID(s) 573058 1258

Special Collections DSMZ CCUG JCM KCTC CIP

History <- ATCC; ATCC 14963 <- H. C. Douglas; UW 228 (Peptococcus aerogenes) <- A. Prevot; BAI (Staphylococcus aerogenes) DSM 20463 <-- ATCC 14963 <-- H. C. Douglas UW 228 <-- A. Prévot BAI. CIP <- 1974, M. Véron, Necker Hosp., Paris, France <- ATCC <- H.C. Douglas: strain UW 228, Peptococcus aerogenes <- A.R. Prévot: strain BAI, Staphylococcus aerogenes

Links

Peptoniphilus asaccharolyticus BAI is an anaerobe, mesophilic, Gram-positive prokaryote that was isolated from Human clinical specimen.

Gram-positive coccus-shaped anaerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 389401

ATCC 14963,DSM 20463,NCIB 10074,NCTC 11461,CCUG 9988,JCM 8143,NCIMB 10074,CIP 74.17,KCTC 3321,NCTC 13151,VTT E-981052,JCM 1765

@ref 20215

Last LPSN update 2026-02-09 11:22:21

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Tissierellales

Family Peptoniphilaceae

Genus Peptoniphilus

Species Peptoniphilus asaccharolyticus

Full scientific name Peptoniphilus asaccharolyticus (Distaso 1912) Ezaki et al. 2001

Synonyms (4)

Peptococcus asaccharolyticus
"Schleiferella asaccharolytica"
"Staphylococcus asaccharolyticus"
Peptostreptococcus asaccharolyticus

BacDive ID	Other strains from **Peptoniphilus asaccharolyticus** (5)	Type strain
148273	P. asaccharolyticus CCUG 35131
150894	P. asaccharolyticus CCUG 42643
151245	P. asaccharolyticus CCUG 43533
151398	P. asaccharolyticus CCUG 44041 A
151462	P. asaccharolyticus CCUG 44107 A

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
116519	positive	coccus-shaped

Multimedia

@ref:	66793
multimedia content:	EM_DSM_20463_1.jpg
multimedia.multimedia content:	EM_DSM_20463_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8834	CHOPPED MEAT MEDIUM (DSMZ Medium 78)	Medium recipe at MediaDive	Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
37334	MEDIUM 20 - for Anaerobic bacteria		Agar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
8834	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
116519	CIP Medium 20	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8834	positive	growth	37	mesophilic
37334	positive	growth	37	mesophilic
45245	positive	growth	37	mesophilic
67770	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
8834	anaerobe
45245	anaerobe
116519	anaerobe
125438	anaerobe	96.87
125439	anaerobe	99.4

Murein

@ref	Murein short key	Type
8834	A21.05	A4ß L-Orn-D-Glu

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
116519	17632 ChEBI	nitrate	+	reduction
116519	16301 ChEBI	nitrite	-	reduction
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
68380	27897 ChEBI	tryptophan	+	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A
116519	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	+	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68380	alpha-glucosidase	-	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
116519	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	-		from API zym
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	L-arginine arylamidase	+		from API rID32A
68382	leucine arylamidase	+	3.4.11.1	from API zym
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
116519	oxidase	-
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	-	3.4.21.4	from API zym
68380	tryptophan deaminase	+	4.1.99.1	from API rID32A
116519	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
116519	-	+	+	-	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
8834	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+/-	+	-	-	-	-	-	+	-	+	+	-	-
8834	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+/-	+	-	-	-	-	-	+/-	-	-	+/-	-	-

Isolation, sampling and environmental information

Isolation

67770

Sample typeHuman clinical specimen

Taxonmaps

69479

Global distribution of 16S sequence AB640690 (>99% sequence identity) for Peptoniphilus from Microbeatlas

Aquatic Samples	12
Soil Samples	12
Animal Samples	1098
Plant Samples	2
Total Samples	1124

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8834	2	Risk group (German classification) According to TRBA 466
116519	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2503982046 annotated assembly for Peptoniphilus asaccharolyticus DSM 20463	contig	GCA_900176115	573058.3	2503982046	573058	75.16
124043	ASM1679160v1 assembly for Peptoniphilus asaccharolyticus FDAARGOS_1135	contig	GCA_016791605	1258.4	8067610335	1258	68.76

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8834	Peptoniphilus asaccharolyticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1765	AB640690	1490		1258
67770	Peptoniphilus asaccharolyticus strain CCUG 9988 16S ribosomal RNA gene, partial sequence	AF542228	1396		573058

GC content

@ref	GC-content (mol%)	Method
8834	32.0
67770	32	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Peptoniphilus asaccharolyticus DSM 20463
NCBI: GCA_900176115

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	65.80	no
125439	motility	BacteriaNetⓘ	yes	53.10	no
125439	gram_stain	BacteriaNetⓘ	positive	74.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Peptoniphilus asaccharolyticus DSM 20463 strain DSM 20463
PATRIC: 573058.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	84.58	no
125438	anaerobic	anaerobicⓘ	yes	96.87	yes
125438	spore-forming	spore-formingⓘ	no	67.09	no
125438	aerobic	aerobicⓘ	no	97.74	yes
125438	thermophilic	thermophileⓘ	no	93.90	yes
125438	flagellated	motile2+ⓘ	no	87.14	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	A reductive uric acid degradation pathway in anaerobic bacteria.	Li Z, Meng W, Gao Z, Peng W, Hu Z, Zhang J, Wang Y, Wu X, Zhao Z, Zhang C, Tang Z, Nie Z, Wu S, Wu B, Zheng H, Luo D, Tong Y, Hu Y, Hu Z, Wei Y, Zhang Y.	Life Metab	10.1093/lifemeta/loaf031	2025
Phylogeny	The completely annotated genome and comparative genomics of the Peptoniphilaceae bacterium str. ING2-D1G, a novel acidogenic bacterium isolated from a mesophilic biogas reactor.	Tomazetto G, Hahnke S, Langer T, Wibberg D, Blom J, Maus I, Puhler A, Klocke M, Schluter A.	J Biotechnol	10.1016/j.jbiotec.2017.05.027	2017
	Conversion of Ultrasmall Glutathione-Coated Silver Nanoparticles during Dispersion in Water into Ultrasmall Silver Sulfide Nanoparticles.	Wolff N, Prymak O, Bialas N, Schaller T, Loza K, Niemeyer F, Heggen M, Weidenthaler C, Oliveira CLP, Epple M.	Nanomaterials (Basel)	10.3390/nano14171449	2024
	Increased Cytotoxicity of Bimetallic Ultrasmall Silver-Platinum Nanoparticles (2 nm) on Cells and Bacteria in Comparison to Silver Nanoparticles of the Same Size.	Wolff N, Bialas N, Loza K, Heggen M, Schaller T, Niemeyer F, Weidenthaler C, Beuck C, Bayer P, Prymak O, Oliveira CLP, Epple M.	Materials (Basel)	10.3390/ma17153702	2024
Metabolism	Phenotypic and genotypic properties of Staphylococcus aureus subsp. anaerobius isolated from lymph node abscesses of goats.	Szalus-Jordanow O, Kanbar T, Soedarmanto I, Eissa N, Alber J, Lammler C, Zschock M, Weiss R, Kaba J, Frymus T.	Berl Munch Tierarztl Wochenschr		2011
	Gene Expression Correlates with Process Rates Quantified for Sulfate- and Fe(III)-Reducing Bacteria in U(VI)-Contaminated Sediments.	Akob DM, Lee SH, Sheth M, Kusel K, Watson DB, Palumbo AV, Kostka JE, Chin KJ.	Front Microbiol	10.3389/fmicb.2012.00280	2012
Phylogeny	Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages.	Cocolin L, Manzano M, Cantoni C, Comi G.	Appl Environ Microbiol	10.1128/aem.67.11.5113-5121.2001	2001
Enzymology	Time-to-detection of bacteria and yeast with the BACTEC FX versus BacT/Alert Virtuo blood culture systems.	Somily AM, Habib HA, Torchyan AA, Sayyed SB, Absar M, Al-Aqeel R, Binkhamis AK.	Ann Saudi Med	10.5144/0256-4947.2018.194	2018
	Quantitative survival of aerobic and anaerobic microorganisms in Port-A-Cul and Copan transport systems.	Stoner KA, Rabe LK, Austin MN, Meyn LA, Hillier SL.	J Clin Microbiol	10.1128/jcm.00161-08	2008
Pathogenicity	In Vitro Antibacterial Properties of Cefiderocol, a Novel Siderophore Cephalosporin, against Gram-Negative Bacteria.	Ito A, Sato T, Ota M, Takemura M, Nishikawa T, Toba S, Kohira N, Miyagawa S, Ishibashi N, Matsumoto S, Nakamura R, Tsuji M, Yamano Y.	Antimicrob Agents Chemother	10.1128/aac.01454-17	2018
Phylogeny	Development of a flow chart for identification of gram-positive anaerobic cocci in the clinical laboratory.	Song Y, Liu C, Finegold SM.	J Clin Microbiol	10.1128/jcm.01872-06	2007
Phylogeny	Bacterial communities in women with bacterial vaginosis: high resolution phylogenetic analyses reveal relationships of microbiota to clinical criteria.	Srinivasan S, Hoffman NG, Morgan MT, Matsen FA, Fiedler TL, Hall RW, Ross FJ, McCoy CO, Bumgarner R, Marrazzo JM, Fredricks DN.	PLoS One	10.1371/journal.pone.0037818	2012
	Peptoniphilus equinus sp. nov., a novel Gram-stain-positive anaerobic coccus isolated from the faeces of a thoroughbred racehorse.	Jung HS, Yun JU, Jung MJ, Song HS, Kim YB, Kim Y, Kim JG, Jeon CO, Roh SW, Whon TW, Lee SH.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006053	2023
Genetics	Peptoniphilus coli sp. nov. and Peptoniphilus urinae sp. nov., isolated from humans.	Mbaye B, Lo CI, Dione N, Benabdelkader S, Tidjani Alou M, Brahimi S, Armstrong N, Alibar S, Raoult D, Moal V, Million M, Fournier PE, Fenollar F.	Arch Microbiol	10.1007/s00203-022-03044-z	2022
Phylogeny	Description of three new Peptoniphilus species cultured in the vaginal fluid of a woman diagnosed with bacterial vaginosis: Peptoniphilus pacaensis sp. nov., Peptoniphilus raoultii sp. nov., and Peptoniphilus vaginalis sp. nov.	Diop K, Diop A, Michelle C, Richez M, Rathored J, Bretelle F, Fournier PE, Fenollar F.	Microbiologyopen	10.1002/mbo3.661	2019
Genetics	Urinicoccus massiliensis gen. nov., sp. nov., a new bacterium isolated from a human urine sample from a 7-year-old boy hospitalized for dental care.	Yimagou EK, Anani H, Yacouba A, Hasni I, Baudoin JP, Raoult D, Bou Khalil JY.	New Microbes New Infect	10.1016/j.nmni.2019.100615	2019
Phylogeny	Peptoniphilus mikwangii sp. nov., isolated from a clinical specimen of human origin.	Cho E, Park SN, Shin Y, Lim YK, Paek J, Kim HK, Hwang CH, Jo E, Jin D, Chang YH, Kook JK	Curr Microbiol	10.1007/s00284-014-0712-7	2014

References

#8834	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20463
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#37334	; Curators of the CIP;
#45245	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 9988
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116519	Collection of Institut Pasteur ; Curators of the CIP; CIP 74.17
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Peptoniphilus asaccharolyticus (DSM 20463, ATCC 14963, NCIB 10074)

Name and taxonomic classification

Morphology

Cell morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API rID32A

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900176115

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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