Strain identifier

BacDive ID: 17996

Type strain: Yes

Species: Peptoniphilus asaccharolyticus

Strain Designation: BAI, UW 228

Strain history: CIP <- 1974, M. Véron, Necker Hosp., Paris, France <- ATCC <- H.C. Douglas: strain UW 228, Peptococcus aerogenes <- A.R. Prévot: strain BAI, Staphylococcus aerogenes

NCBI tax ID(s): 573058 (strain), 1258 (species)

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General

@ref: 8834

BacDive-ID: 17996

DSM-Number: 20463

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, coccus-shaped

description: Peptoniphilus asaccharolyticus BAI is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from Human clinical specimen.

NCBI tax id

NCBI tax idMatching level
1258species
573058strain

strain history

@refhistory
8834<- ATCC; ATCC 14963 <- H. C. Douglas; UW 228 (Peptococcus aerogenes) <- A. Prevot; BAI (Staphylococcus aerogenes)
67770DSM 20463 <-- ATCC 14963 <-- H. C. Douglas UW 228 <-- A. Prévot BAI.
116519CIP <- 1974, M. Véron, Necker Hosp., Paris, France <- ATCC <- H.C. Douglas: strain UW 228, Peptococcus aerogenes <- A.R. Prévot: strain BAI, Staphylococcus aerogenes

doi: 10.13145/bacdive17996.20250331.9.3

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Peptoniphilaceae
  • genus: Peptoniphilus
  • species: Peptoniphilus asaccharolyticus
  • full scientific name: Peptoniphilus asaccharolyticus (Distaso 1912) Ezaki et al. 2001

  • synonyms

    @refsynonym
    20215Peptostreptococcus asaccharolyticus
    20215Staphylococcus asaccharolyticus
    20215Schleiferella asaccharolytica
    20215Peptococcus asaccharolyticus

@ref: 8834

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Peptoniphilaceae

genus: Peptoniphilus

species: Peptoniphilus asaccharolyticus

full scientific name: Peptoniphilus asaccharolyticus (Distaso 1912) Ezaki et al. 2001

strain designation: BAI, UW 228

type strain: yes

Morphology

cell morphology

  • @ref: 116519
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

multimedia

  • @ref: 66793
  • multimedia content: EM_DSM_20463_1.jpg
  • caption: electron microscopic image
  • intellectual property rights: © HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8834CHOPPED MEAT MEDIUM (DSMZ Medium 78)yeshttps://mediadive.dsmz.de/medium/78Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water
37334MEDIUM 20 - for Anaerobic bacteriayesAgar (15.000 g);Glucose (5.000 g);Yeast extract (20.000 g);Tryptone (30.000 g);Cysteine hydrochloride (0.500 g);distilled water (1000.000 ml);Hemin solution -M00149 (25.000 ml)
8834COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium693.pdf
116519CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperature
8834positivegrowth37
37334positivegrowth37
45245positivegrowth37
67770positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen toleranceconfidence
8834anaerobe
45245anaerobe
116519anaerobe
125438anaerobe96.87
125439anaerobe99.4

murein

  • @ref: 8834
  • murein short key: A21.05
  • type: A4ß L-Orn-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838017632nitrate-reduction
6838027897tryptophan+energy source
6838029985L-glutamate-degradation
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838016199urea-hydrolysis
11651917632nitrate+reduction
11651916301nitrite-reduction

metabolite production

@refChebi-IDmetaboliteproduction
11651935581indoleyes
6838035581indoleyes

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382lipase (C 14)-
68382esterase (C 4)+
68382esterase lipase (C 8)-
68382alkaline phosphatase+3.1.3.1
116519oxidase-
116519catalase-1.11.1.6
116519urease-3.5.1.5
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68380alanine arylamidase-3.4.11.2
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase+
68380tryptophan deaminase+4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380beta-glucosidase-3.2.1.21
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116519-++--+----+---------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8834-+-------------++/-+-----+-++--
8834---------------++/-+-----+/---+/---

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Human clinical specimen

taxonmaps

  • @ref: 69479
  • File name: preview.99_7205.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_38;96_3367;97_4135;98_5291;99_7205&stattab=map
  • Last taxonomy: Peptoniphilus
  • 16S sequence: AB640690
  • Sequence Identity:
  • Total samples: 1124
  • soil counts: 12
  • aquatic counts: 12
  • animal counts: 1098
  • plant counts: 2

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88342Risk group (German classification)
1165191Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
8834Peptoniphilus asaccharolyticus gene for 16S ribosomal RNA, partial sequence, strain: JCM 1765AB6406901490nuccore1258
67770Peptoniphilus asaccharolyticus strain CCUG 9988 16S ribosomal RNA gene, partial sequenceAF5422281396nuccore573058

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Peptoniphilus asaccharolyticus DSM 20463 strain DSM 20463573058.3wgspatric573058
66792Peptoniphilus asaccharolyticus strain FDAARGOS_11351258.4wgspatric1258
66792Peptoniphilus asaccharolyticus UW 228, DSM 204632503982046draftimg573058
67770Peptoniphilus asaccharolyticus DSM 20463GCA_900176115contigncbi573058

GC content

@refGC-contentmethod
883432.0
6777032thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

@refmodeltraitdescriptionpredictionconfidencetraining_data
125438gram-positivegram-positivePositive reaction to Gram-stainingyes84.583no
125438anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)yes96.87yes
125438spore-formingspore-formingAbility to form endo- or exosporesno67.085no
125438aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no97.743yes
125438thermophilethermophilicAbility to grow at temperatures above or equal to 45°Cno93.901yes
125438motile2+flagellatedAbility to perform flagellated movementno87.143no
125439BacteriaNetspore_formationAbility to form endo- or exosporesno65.8
125439BacteriaNetmotilityAbility to perform movementyes53.1
125439BacteriaNetgram_stainReaction to gram-stainingpositive74.6
125439BacteriaNetoxygen_toleranceOxygenic conditions needed for growthanaerobe99.4

External links

@ref: 8834

culture collection no.: DSM 20463, ATCC 14963, NCIB 10074, CCUG 9988, JCM 1765, CIP 74.17, KCTC 3321, NCIMB 10074, NCTC 11461, VTT E-981052

straininfo link

  • @ref: 86983
  • straininfo: 389401

literature

  • topic: Phylogeny
  • Pubmed-ID: 25319028
  • title: Peptoniphilus mikwangii sp. nov., isolated from a clinical specimen of human origin.
  • authors: Cho E, Park SN, Shin Y, Lim YK, Paek J, Kim HK, Hwang CH, Jo E, Jin D, Chang YH, Kook JK
  • journal: Curr Microbiol
  • DOI: 10.1007/s00284-014-0712-7
  • year: 2014
  • mesh: Base Composition, Fatty Acids/chemistry, Gram-Positive Bacteria/chemistry/*classification/genetics/isolation & purification, Humans, Male, Middle Aged, Phenotype, Phylogeny, RNA, Ribosomal, 16S/*genetics
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8834Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20463)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20463
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37334Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10708
45245Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 9988)https://www.ccug.se/strain?id=9988
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
86983Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID389401.1StrainInfo: A central database for resolving microbial strain identifiers
116519Curators of the CIPCollection of Institut Pasteur (CIP 74.17)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2074.17
125438Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann10.1101/2024.08.12.607695Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets
125439Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardyhttps://github.com/GenomeNet/deepGdeepG: Deep Learning for Genome Sequence Data. R package version 0.3.1