Name: Helcococcus kunzii No. 22
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17990

Type strain:

Species: Helcococcus kunzii

Strain Designation: No. 22, 22

Culture col. no.: DSM 10548, ATCC 51366, IFO 15552, NBRC 15552, NCFB 2900, CCUG 32213, CIP 103932

Strain history: CIP <- 1993, K.L. Ruoff, Microbiol. Lab. Massachusetts Gen. Hosp., Boston, USA: strain 22

NCBI tax ID(s): 883114 (strain), 40091 (species)

SI-ID 8997

LMG 15123, ATCC 51366, CCUG 32213, CIP 103932, DSM 10548, IFO 15552, NCFB 2900, NCIMB 702900, CCM4547, NCIMB 13850, NBRC 15552

Other strains from Helcococcus kunzii

H. kunzii CCUG 30187
H. kunzii CCUG 31183 A
H. kunzii CCUG 31225
H. kunzii CCUG 31742
H. kunzii
H. kunzii CCUG 42037
H. kunzii CCUG 42366
H. kunzii CCUG 44832
H. kunzii CCUG 45180 A
H. kunzii CCUG 45180 B
H. kunzii CCUG 45831 B
H. kunzii CCUG 50407
H. kunzii CCUG 50466
H. kunzii CCUG 61088 A
H. kunzii CCUG 69101

Section

Helcococcus kunzii No. 22 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from human wound.

coccus-shaped
Gram-positive
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Peptoniphilaceae*
	Genus	*Helcococcus*
	Species	**Helcococcus kunzii**
	Full Scientific Name (LPSN)	Helcococcus kunzii Collins et al. 1993

Information on morphological and physiological properties Morphology

[Ref.: #120514]	Gram stain	positive

[Ref.: #120514]	Cell shape	coccus-shaped

[Ref.: #120514]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3922]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3922]

Culture medium growth

[Ref.: #3922]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3922]

Culture medium composition

expand / minimize

[Ref.: #40876]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #40876]

Culture medium growth

[Ref.: #40876]

Culture medium composition

expand / minimize

[Ref.: #120514]

Culture medium

CIP Medium 6

[Ref.: #120514]

Culture medium growth

[Ref.: #120514]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3922]	growth	30 °C
[Ref.: #40876]	growth	37 °C
[Ref.: #50902]	growth	37 °C
[Ref.: #120514]	growth	37 °C

[Ref.: #3922]	Temperature range	mesophilic
[Ref.: #120514]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #50902]	Oxygen tolerance	microaerophile
[Ref.: #120514]	Oxygen tolerance	facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #120514]		NaCl		growth	6.5	%

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120514]		nitrate ChEBI	-	reduction
[Ref.: #120514]		nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120514]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #120514]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #120514]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #120514]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #120514]	beta-galactosidase	+	3.2.1.23
[Ref.: #120514]	catalase	-	1.11.1.6
[Ref.: #120514]	lysine decarboxylase	-	4.1.1.18
[Ref.: #120514]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #120514]	urease	-	3.5.1.5

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	ppGpp biosynthesis	100	4 of 4
[Ref.: #66794]	starch degradation	100	10 of 10
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	L-lactaldehyde degradation	100	3 of 3
[Ref.: #66794]	palmitate biosynthesis	81.82	18 of 22
[Ref.: #66794]	glycogen metabolism	80	4 of 5
[Ref.: #66794]	aspartate and asparagine metabolism	77.78	7 of 9
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	peptidoglycan biosynthesis	73.33	11 of 15
[Ref.: #66794]	oxidative phosphorylation	67.03	61 of 91
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	formaldehyde oxidation	66.67	2 of 3
[Ref.: #66794]	serine metabolism	66.67	6 of 9
[Ref.: #66794]	photosynthesis	64.29	9 of 14
[Ref.: #66794]	purine metabolism	61.7	58 of 94
[Ref.: #66794]	vitamin B1 metabolism	61.54	8 of 13
[Ref.: #66794]	NAD metabolism	61.11	11 of 18
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	glycine betaine biosynthesis	60	3 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	60	3 of 5
[Ref.: #66794]	Entner Doudoroff pathway	60	6 of 10
[Ref.: #66794]	methionine metabolism	57.69	15 of 26
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	degradation of hexoses	55.56	10 of 18
[Ref.: #66794]	butanoate fermentation	50	2 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	50	2 of 4
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	glycolate and glyoxylate degradation	50	3 of 6
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	C4 and CAM-carbon fixation	50	4 of 8
[Ref.: #66794]	pyrimidine metabolism	48.89	22 of 45
[Ref.: #66794]	non-pathway related	47.37	18 of 38
[Ref.: #66794]	metabolism of disaccharids	45.45	5 of 11
[Ref.: #66794]	d-xylose degradation	45.45	5 of 11
[Ref.: #66794]	chorismate metabolism	44.44	4 of 9
[Ref.: #66794]	cysteine metabolism	44.44	8 of 18
[Ref.: #66794]	degradation of pentoses	42.86	12 of 28
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	mevalonate metabolism	42.86	3 of 7
[Ref.: #66794]	threonine metabolism	40	4 of 10
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	ketogluconate metabolism	37.5	3 of 8
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
[Ref.: #66794]	gluconeogenesis	37.5	3 of 8
[Ref.: #66794]	pentose phosphate pathway	36.36	4 of 11
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	tetrahydrofolate metabolism	35.71	5 of 14
[Ref.: #66794]	alanine metabolism	34.48	10 of 29
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	33.33	4 of 12
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	lipid metabolism	32.26	10 of 31
[Ref.: #66794]	glutamate and glutamine metabolism	32.14	9 of 28
[Ref.: #66794]	degradation of sugar acids	32	8 of 25
[Ref.: #66794]	ascorbate metabolism	31.82	7 of 22
[Ref.: #66794]	degradation of sugar alcohols	31.25	5 of 16
[Ref.: #66794]	phenylpropanoid biosynthesis	30.77	4 of 13
[Ref.: #66794]	leucine metabolism	30.77	4 of 13
[Ref.: #66794]	sulfate reduction	30.77	4 of 13
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	isoprenoid biosynthesis	26.92	7 of 26
[Ref.: #66794]	flavin biosynthesis	26.67	4 of 15
[Ref.: #66794]	tryptophan metabolism	26.32	10 of 38
[Ref.: #66794]	arginine metabolism	25	6 of 24
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	carnitine metabolism	25	2 of 8
[Ref.: #66794]	histidine metabolism	24.14	7 of 29
[Ref.: #66794]	arachidonic acid metabolism	22.22	4 of 18
[Ref.: #66794]	CO2 fixation in Crenarchaeota	22.22	2 of 9
[Ref.: #66794]	valine metabolism	22.22	2 of 9
[Ref.: #66794]	glutathione metabolism	21.43	3 of 14
[Ref.: #66794]	citric acid cycle	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3922]	Sample type/isolated from	human wound
[Ref.: #3922]	Country	USA
[Ref.: #3922]	Country ISO 3 Code	USA
[Ref.: #3922]	Continent	North America

[Ref.: #120514]	Sample type/isolated from	Infected foot ulcer
[Ref.: #120514]	Geographic location (country and/or sea, region)	Boston, Massachusetts
[Ref.: #120514]	Country	United States of America
[Ref.: #120514]	Country ISO 3 Code	USA
[Ref.: #120514]	Continent	North America
[Ref.: #120514]	Isolation date	1990
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Host Body-Site	#Other	#Wound

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X69837 (>99% sequence identity) for Helcococcus kunzii subclade from Microbeatlas

Aquatic Samples	1
Soil Samples	1
Animal Samples	98
Plant Samples
Total Samples	100

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3922]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120514]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3922]	H.kunzii gene for 16S rRNA, partial	X69837	1465	ENA	40091 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Helcococcus kunzii ATCC 51366	GCA_000245755	scaffold	GenBank	883114 tax ID	*
[Ref.: #66792]	Helcococcus kunzii ATCC 51366	883114.8	wgs	PATRIC	883114 tax ID	*
[Ref.: #66792]	Helcococcus kunzii ATCC 51366	883114.3	wgs	PATRIC	883114 tax ID	*
[Ref.: #66792]	Helcococcus kunzii ATCC 51366	2513237357	draft	JGI IMG/M	883114 tax ID	*

[Ref.: #3922]

GC-content

30 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Helcococcus kunzii ATCC 51366 ATCC 51366 NCBI: GCA_000245755.1
[Ref.: #69480]	motile	no	96.319	no
[Ref.: #69480]	flagellated	no	97.674	no
[Ref.: #69480]	gram-positive	yes	92.531	no
[Ref.: #69480]	anaerobic	no	50.97	yes
[Ref.: #69480]	aerobic	no	98.098	yes
[Ref.: #69480]	halophile	yes	53.919	no
[Ref.: #69480]	spore-forming	no	93.01	no
[Ref.: #69480]	glucose-util	yes	86.805	no
[Ref.: #69480]	thermophile	no	97.878	no
[Ref.: #69480]	glucose-ferment	yes	71.121	no

Availability in culture collections External links

[Ref.: #3922]

Culture collection no.

DSM 10548, ATCC 51366, IFO 15552, NBRC 15552, NCFB 2900, CCUG 32213, CIP 103932

[Ref.: #86977]

SI-ID 8997

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Comparative genomics analysis of two Helcococcus kunzii strains co-isolated with Staphylococcus aureus from diabetic foot ulcers.	Durand BARN, Yahiaoui Martinez A, Baud D, Francois P, Lavigne JP, Dunyach-Remy C	Genomics	10.1016/j.ygeno.2022.110365	2022	*
Pathogenicity	In vitro antimicrobial susceptibility of Helcococcus kunzii and molecular analysis of macrolide and tetracycline resistance.	Vergne A, Guerin F, Lienhard R, Le Coustumier A, Daurel C, Isnard C, Marty N, Poyart C, Cattoir V	Eur J Clin Microbiol Infect Dis	10.1007/s10096-015-2451-5	2015	*
Phylogeny	Phylogenetic analysis of some Aerococcus-like organisms from clinical sources: description of Helcococcus kunzii gen. nov., sp. nov.	Collins MD, Facklam RR, Rodrigues UM, Ruoff KL	Int J Syst Bacteriol	10.1099/00207713-43-3-425	1993	*

References

#3922

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10548

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#40876 ; Curators of the CIP;
#50902 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32213

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#86977

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8997.1 )

#120514 Collection of Institut Pasteur ; Curators of the CIP; CIP 103932
* These data were automatically processed and therefore are not curated

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Helcococcus kunzii ATCC 51366 ATCC 51366

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