Methanocella conradii (DSM 24694, CGMCC 1.5162, JCM 17849)

Name: Methanocella conradii (DSM 24694, CGMCC 1.5162, JCM 17849)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 17669

Type strain

Strain Designation HZ254

Culture col. no. DSM 24694 CGMCC 1.5162 JCM 17849 HZ254

NCBI tax ID(s) 1175444

Special Collections DSMZ JCM

History DSMZ <- L. YaHai, China Agricultural University, Beijing, China; HZ254 <- Z. Lü; {2009}

Links

Methanocella conradii HZ254 is an anaerobe archaeon that was isolated from rice paddies.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 401278

DSM 24694

@ref 20215

Last LPSN update 2026-05-29 08:29:04

Domain Archaea

Phylum Methanobacteriota

Class Methanosarcinia

Order Methanocellales

Family Methanocellaceae

Genus Methanocella

Species Methanocella conradii

Full scientific name Methanocella conradii Lü and Lu 2012

Morphology

Colony morphology

17731

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17731	METHANOCELLA CONRADII MEDIUM (DSMZ Medium 1318)		Medium recipe at MediaDive	Name: METHANOCELLA CONRADII MEDIUM (DSMZ Medium 1318) Composition: Na2CO3 0.996016 g/l KCl 0.498008 g/l MgCl2 x 6 H2O 0.398406 g/l DL-Dithiothreitol 0.298805 g/l L-Cysteine HCl x H2O 0.298805 g/l Yeast extract 0.199203 g/l KH2PO4 0.199203 g/l CaCl2 x 2 H2O 0.0996016 g/l NH4Cl 0.0996016 g/l Na-acetate 0.0896414 g/l HCl 0.00249004 g/l FeCl2 x 4 H2O 0.00149402 g/l Sodium resazurin 0.000498008 g/l NaOH 0.000498008 g/l Pyridoxine hydrochloride 0.000298805 g/l Nicotinic acid 0.000199203 g/l Thiamine-HCl x 2 H2O 0.000199203 g/l CoCl2 x 6 H2O 0.000189243 g/l Vitamin B12 9.96016e-05 g/l MnCl2 x 4 H2O 9.96016e-05 g/l Calcium pantothenate 9.96016e-05 g/l p-Aminobenzoic acid 7.96813e-05 g/l ZnCl2 6.97211e-05 g/l Calcium D-(+)-pantothenate 4.98008e-05 g/l (DL)-alpha-Lipoic acid 4.98008e-05 g/l Riboflavin 4.98008e-05 g/l Thiamine HCl 4.98008e-05 g/l Na2MoO4 x 2 H2O 3.58566e-05 g/l NiCl2 x 6 H2O 2.39044e-05 g/l Biotin 1.99203e-05 g/l D-(+)-biotin 1.99203e-05 g/l Folic acid 1.99203e-05 g/l H3BO3 5.9761e-06 g/l Na2WO4 x 2 H2O 3.98406e-06 g/l Na2SeO3 x 5 H2O 2.98805e-06 g/l CuCl2 x 2 H2O 1.99203e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
17731	positive	growth	50

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
17731	anaerobe
125439	obligate anaerobe	99.871

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Agriculture	#Field
#Host	#Plants	#Herbaceous plants (Grass,Crops)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
17731	rice paddies	Hangzhou, China National Rice Res. Institute	China	CHN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17731	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM25110v1 assembly for Methanocella conradii HZ254	complete	GCA_000251105	1041930.6	2512564055	1041930	99.15

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17731	Methanocella conradii HZ254 16S ribosomal RNA gene, partial sequence	JN048683	1422		1041930

GC content

@ref	GC-content (mol%)	Method
17731	52.7	sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM25110v1 assembly for Methanocella conradii HZ254
BV-BRC: 1041930.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate anaerobe	99.87	no
125439	motility	BacteriaNetⓘ	no	70.88	no
125439	gram_stain	BacteriaNetⓘ	positive	45.67	no
125439	spore_formation	BacteriaNetⓘ	no	72.46	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanocella conradii HZ254 HZ254
NCBI: GCA_000251105.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	64.24	no
125438	anaerobic	anaerobicⓘ	yes	84.97	yes
125438	aerobic	aerobicⓘ	no	87.56	no
125438	spore-forming	spore-formingⓘ	no	84.61	no
125438	thermophilic	thermophileⓘ	no	58.36	no
125438	flagellated	motile2+ⓘ	no	86.38	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Stimulatory Effect of Magnetite Nanoparticles on a Highly Enriched Butyrate-Oxidizing Consortium.	Fu L, Song T, Zhang W, Zhang J, Lu Y.	Front Microbiol	10.3389/fmicb.2018.01480	2018
Metabolism	Response of a rice paddy soil methanogen to syntrophic growth as revealed by transcriptional analyses.	Liu P, Yang Y, Lu Z, Lu Y	Appl Environ Microbiol	10.1128/AEM.01259-14	2014
Genetics	Complete genome sequence of a thermophilic methanogen, Methanocella conradii HZ254, isolated from Chinese rice field soil.	Lu Z, Lu Y	J Bacteriol	10.1128/JB.00207-12	2012
Phylogeny	Methanocella conradii sp. nov., a thermophilic, obligate hydrogenotrophic methanogen, isolated from Chinese rice field soil.	Lu Z, Lu Y	PLoS One	10.1371/journal.pone.0035279	2012

References

#17731	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24694
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanocella conradii (DSM 24694, CGMCC 1.5162, JCM 17849)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000251105

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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