Name: Vibrio ichthyoenteri F-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 17289

Type strain:

Species: Vibrio ichthyoenteri

Strain Designation: F-2

Culture col. no.: DSM 14397, CECT 5675, IFO 15847, NBRC 15847, CCUG 57206, NCIMB 13441, LMG 19664, CIP 104815, ATCC 700023

Strain history: CIP <- 1996, K. Muroga, Hiroshima Univ., Hiroshima, Japan: strain F-2 <- K. Muroga

NCBI tax ID(s): 870968 (strain), 142461 (species)

SI-ID 12997

LMG 19147, IFO 15847, ATCC 700023, CIP 104815, DSM 14397, NCIMB 13441, LMG 19664, CECT 5675, NBRC 15847

Section

Vibrio ichthyoenteri F-2 is a facultative anaerobe, mesophilic, Gram-negative animal pathogen that was isolated from gut of diseased Japanese flounder larvae.

Gram-negative
motile
rod-shaped
animal pathogen
facultative anaerobe
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	"Vibrionales"
	Family	*Vibrionaceae*
	Genus	*Vibrio*
	Species	**Vibrio ichthyoenteri**
	Full Scientific Name (LPSN)	Vibrio ichthyoenteri Ishimaru et al. 1996

Information on morphological and physiological properties Morphology

[Ref.: #120040]	Gram stain	negative

[Ref.: #120040]	Cell shape	rod-shaped

[Ref.: #120040]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5334]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #5334]

Culture medium growth

[Ref.: #5334]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5334]

Culture medium composition

expand / minimize

[Ref.: #36940]

Culture medium

Marine agar (MA)

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Culture medium growth

[Ref.: #36940]

Culture medium composition

expand / minimize

[Ref.: #120040]

Culture medium

CIP Medium 13

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5334]		growth	28 °C
[Ref.: #36940]		growth	25 °C
[Ref.: #120040]		growth	15-30 °C
[Ref.: #120040]	no	growth	5 °C
[Ref.: #120040]	no	growth	37 °C
[Ref.: #120040]	no	growth	41 °C

[Ref.: #5334]	Temperature range	mesophilic
[Ref.: #36940]	Temperature range	mesophilic
[Ref.: #120040]	Temperature range	psychrophilic
[Ref.: #120040]	Temperature range	mesophilic
[Ref.: #120040]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #120040]

Oxygen tolerance

facultative anaerobe

	Salt	Tested relation		Salt conc.
[Ref.: #120040]	NaCl		growth	2-6	%
[Ref.: #120040]	NaCl	no	growth	0	%
[Ref.: #120040]	NaCl	no	growth	8	%
[Ref.: #120040]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #120040]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E
[Ref.: #68368]	D-mannitol ChEBI	+	fermentation	* from API 20E
[Ref.: #120040]	esculin ChEBI	-	hydrolysis
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #120040]	glucose ChEBI	-	fermentation
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #120040]	lactose ChEBI	-	fermentation
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #120040]	nitrate ChEBI	+	reduction
[Ref.: #120040]	nitrate ChEBI	+	respiration
[Ref.: #120040]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #120040]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #120040]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite	Production
[Ref.: #120040]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #120040]	glucose ChEBI		-
[Ref.: #68368]	acetoin ChEBI	-			* from API 20E
[Ref.: #68368]	indole ChEBI			-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #120040]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #120040]	amylase	+
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #120040]	beta-galactosidase	-	3.2.1.23
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #120040]	caseinase	-	3.4.21.50
[Ref.: #120040]	catalase	+	1.11.1.6
[Ref.: #68368]	cytochrome oxidase	+	1.9.3.1	* from API 20E
[Ref.: #120040]	DNase	-
[Ref.: #120040]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #120040]	gelatinase	-
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #120040]	lecithinase	-
[Ref.: #120040]	lipase	-
[Ref.: #120040]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #120040]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #120040]	oxidase	+
[Ref.: #120040]	phenylalanine ammonia-lyase	+	4.3.1.24
[Ref.: #120040]	protease	-
[Ref.: #120040]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #120040]	tween esterase	-
[Ref.: #120040]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #5334]	16589	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5334]	Sample type/isolated from	gut of diseased Japanese flounder larvae
[Ref.: #5334]	Geographic location (country and/or sea, region)	western part
[Ref.: #5334]	Country	Japan
[Ref.: #5334]	Country ISO 3 Code	JPN
[Ref.: #5334]	Continent	Asia

[Ref.: #120040]	Sample type/isolated from	Japanese flounder, Paralichthys olivaceus
[Ref.: #120040]	Geographic location (country and/or sea, region)	Hiroshima
[Ref.: #120040]	Country	Japan
[Ref.: #120040]	Country ISO 3 Code	JPN
[Ref.: #120040]	Continent	Asia
[Ref.: #120040]	Isolation date	1986
* marker position based on {}

Isolation sources categories

#Host	#Fishes	-
#Host Body-Site	#Gastrointestinal tract	#Stomach

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HM771339 (>99% sequence identity) for Vibrio from Microbeatlas

Aquatic Samples	1316
Soil Samples	44
Animal Samples	788
Plant Samples	11
Total Samples	2159

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5334]	Pathogenicity (animal)	yes
[Ref.: #5334]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120040]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Vibrio ichthyoenteri ATCC 700023 16S ribosomal RNA gene, partial sequence	HM771339	1465	ENA	870968 tax ID	*
[Ref.: #20218]	Vibrio ichthyoenteri 16S rRNA gene, strain DSM 14397T	AJ421445	1367	ENA	870968 tax ID	*
[Ref.: #20218]	Vibrio ichthyoenteri DNA, 16S-23S rRNA intergenic spacer, partial sequence, strain: IFO 15847	AB255722	227	ENA	870968 tax ID	*
[Ref.: #20218]	Vibrio ichthyoenteri partial 16S rRNA gene, strain LMG 19664T	AJ437192	1462	ENA	870968 tax ID	*
[Ref.: #20218]	Vibrio ichthyoenteri gene for 16S rRNA, partial sequence, strain: NBRC 15847	AB680983	1472	ENA	870968 tax ID	*

[Ref.: #5334]

GC-content

43-44 mol%

Availability in culture collections External links

[Ref.: #5334]

Culture collection no.

DSM 14397, CECT 5675, IFO 15847, NBRC 15847, CCUG 57206, NCIMB 13441, LMG 19664, CIP 104815, ATCC 700023

[Ref.: #86289]	SI-ID 12997	*
[Ref.: #86290]	SI-ID 409729	*

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Detection of quorum sensing signal molecules and mutation of luxS gene in Vibrio ichthyoenteri.	Li X, Han Y, Yang Q, Zhang XH	Res Microbiol	10.1016/j.resmic.2009.10.004	2009	*
Phylogeny	Vibrio sinensis sp. nov. and Vibrio viridaestus sp. nov., two marine bacteria isolated from the East China Sea.	Li Y, Liang J, Liu R, Xue CX, Zhou S, He X, Li B, Wang X, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijsem.0.003842	2020	*

References

#5334

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14397

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36940 ; Curators of the CIP;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#86289

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID12997.1 )

#86290

#120040 Collection of Institut Pasteur ; Curators of the CIP; CIP 104815
* These data were automatically processed and therefore are not curated

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