Name: Actinomadura pelletieri A19
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 16950

Type strain:

Species: Actinomadura pelletieri

Strain Designation: A19

Culture col. no.: DSM 43383, ATCC 33385, JCM 3388, NCTC 4162, CCUG 38891, CIP 105483, HUT 6549, IAM 12634, IMET 9693, IMSNU 22169, KCTC 9110, NBRC 103052, NRRL B-3997

Strain history: CIP <- 1998, JCM <- DSM <- M. Goodfellow: strain A19 <- NCTC, Nocardia pelletieri <- J.T. Duncan <- E.C. Smith

NCBI tax ID(s): 1120940 (strain), 111805 (species)

SI-ID 40471

ATCC 33385, NCTC 4162, CCUG 38891, DSM 43383, CIP 105483, HUT 6549, IAM 12634, IMET 9693, JCM 3388, NRRL B-3997, KCTC 9110, NBRC 103052

Special Collections

Other strains from Actinomadura pelletieri

Section

Actinomadura pelletieri A19 is an obligate aerobe, spore-forming bacterium that was isolated from mycetoma of arm.

spore-forming
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura pelletieri**
	Full Scientific Name (LPSN)	Actinomadura pelletieri (Laveran 1906) Lechevalier and Lechevalier 1970 (Approved Lists 1980)

Synonym

"Micrococcus pelletieri"

Information on morphological and physiological properties Morphology

[Ref.: #121655]	Gram stain	negative

[Ref.: #121655]	Cell shape	rod-shaped

[Ref.: #121655]	Motility	no

Multimedia content

[Ref.: #11000]

Intellectual property rights:

[Ref.: #11000]

Caption:

Medium 554 28°C

[Ref.: #11000]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #39301]

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

[Ref.: #39301]

Culture medium growth

[Ref.: #39301]

Culture medium composition

expand / minimize

[Ref.: #11000]

Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

[Ref.: #11000]

Culture medium growth

[Ref.: #11000]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11000]

Culture medium composition

expand / minimize

[Ref.: #121655]

Culture medium

CIP Medium 57

[Ref.: #121655]

Culture medium growth

[Ref.: #121655]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #121655]

Culture medium

CIP Medium 236

[Ref.: #121655]

Culture medium growth

[Ref.: #121655]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #11000]		growth	28 °C
[Ref.: #18490]		optimum	28 °C
[Ref.: #39301]		growth	30 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #121655]		growth	22-41 °C
[Ref.: #121655]	no	growth	5 °C
[Ref.: #121655]	no	growth	15 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121655]	Oxygen tolerance	obligate aerobe
[Ref.: #69480]	Oxygen tolerance	aerobe Confidence in %93.065

[Ref.: #69481]

Ability of spore formation

yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #18490]	NaCl		maximum	2.5	%
[Ref.: #121655]	NaCl		growth	0	%
[Ref.: #121655]	NaCl	no	growth	2	%
[Ref.: #121655]	NaCl	no	growth	4	%
[Ref.: #121655]	NaCl	no	growth	6	%
[Ref.: #121655]	NaCl	no	growth	8	%
[Ref.: #121655]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-9(H₈), MK-9(H₁₀), MK-9(H₆), MK-9(H₈), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18490]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #18490]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #121655]	esculin ChEBI	-	hydrolysis
[Ref.: #18490]	fructose ChEBI	-
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18490]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #18490]	mannitol ChEBI	-
[Ref.: #18490]	myo-inositol ChEBI	-
[Ref.: #121655]	nitrate ChEBI	+	reduction
[Ref.: #121655]	nitrate ChEBI	-	respiration
[Ref.: #121655]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #18490]	raffinose ChEBI	-
[Ref.: #18490]	rhamnose ChEBI	-
[Ref.: #18490]	sucrose ChEBI	-
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E
[Ref.: #18490]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #121655]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #121655]	acetoin ChEBI	-
[Ref.: #121655]	glucose ChEBI		-
[Ref.: #68368]	acetoin ChEBI	+			* from API 20E
[Ref.: #68368]	indole ChEBI			-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121655]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121655]	amylase	-
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #121655]	beta-galactosidase	-	3.2.1.23
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E, API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121655]	caseinase	+	3.4.21.50
[Ref.: #121655]	catalase	+	1.11.1.6
[Ref.: #121655]	DNase	-
[Ref.: #121655]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #121655]	gelatinase	+
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #121655]	lecithinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121655]	lipase	+
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121655]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121655]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #121655]	oxidase	-
[Ref.: #121655]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #121655]	protease	+
[Ref.: #121655]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #121655]	tween esterase	+
[Ref.: #121655]	urease	-	3.5.1.5
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #54533]

Fatty acid	Percentage	ECL
C16:0 iso	74.7	15.626
C17:0 10-methyl	5.8	17.41
C16:0	5.5	16
C18:0 ISO	5.5	17.632
C18:1 ω9c	4.7	17.769
TBSA 10Me18:0	3.9	18.392

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18490]	72	-	+	+	+	+	-	+	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18490]	187	not determinedn.d.	+	-	-	-	+	+	+	+	+	+	+	-	-	-	-	-	+	-	-
[Ref.: #121655]	8736	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11000]	Sample type/isolated from	mycetoma of arm

[Ref.: #54533]	Sample type/isolated from	Mycetoma of arm

[Ref.: #67770]	Sample type/isolated from	Mycetoma of the arm

[Ref.: #121655]	Sample type/isolated from	Mycetoma of arm
[Ref.: #121655]	Country	Nigeria
[Ref.: #121655]	Country ISO 3 Code	NGA
[Ref.: #121655]	Continent	Africa
* marker position based on {}

Isolation sources categories

#Infection	#Disease	-
#Host Body-Site	#Limb	#Arm

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ293710 (>99% sequence identity) for Actinomadura pelletieri subclade from Microbeatlas

Aquatic Samples	4
Soil Samples	109
Animal Samples	5
Plant Samples	31
Total Samples	149

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18490]	Biosafety level	2	German classification
[Ref.: #11000]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121655]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinomadura pelletieri partial 16S rRNA gene, strain IMSNU 22169T	AJ293710	1479	GenBank ENA	111805 tax ID	*
[Ref.: #20218]	Actinomadura pelletieri 16S ribosomal RNA gene, partial sequence	AF163119	1451	GenBank ENA	111805 tax ID	*
[Ref.: #20218]	Actinomadura pelletieri 16S-23S ribosomal RNA intergenic spacer sequence	AF163130	467	GenBank ENA	111805 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Actinomadura pelletieri DSM 43383	GCA_003634705	scaffold	GenBank ENA	1120940 tax ID
[Ref.: #66792]	Actinomadura pelletieri DSM 43383	1120940.3	wgs	PATRIC	1120940 tax ID	*
[Ref.: #66792]	Actinomadura pelletieri DSM 43383	2728369498	draft	JGI IMG/M	111805 tax ID	*

[Ref.: #67770]	GC-content	70.4 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	66 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinomadura pelletieri DSM 43383 DSM 43383 NCBI: GCA_003634705.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomadura pelletieri DSM 43383 PATRIC: 1120940.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	87.628	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	94.371	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	85.416	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	93.065	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	88.8	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	85.524	no

Availability in culture collections External links

[Ref.: #11000]

Culture collection no.

DSM 43383, ATCC 33385, JCM 3388, NCTC 4162, CCUG 38891, CIP 105483, HUT 6549, IAM 12634, IMET 9693, IMSNU 22169, KCTC 9110, NBRC 103052, NRRL B-3997

[Ref.: #85955]

SI-ID 40471

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura soli sp. nov., isolated from the top soil layer on basaltic material in Turkey.	Saricaoglu S, Saygin H, Topkara AR, Gencbay T, Guven K, Cetin D, Sahin N, Isik K	Int J Syst Evol Microbiol	10.1099/ijsem.0.005062	2021	*
Phylogeny	Actinomadura meridiana sp. nov., isolated from mountain soil.	Lee SD	Int J Syst Evol Microbiol	10.1099/ijs.0.029546-0	2011	*
Phylogeny	Actinomadura spongiicola sp. nov., isolated from the marine sponge Leucetta chagosensis.	Qin Q, Jiang L, Zhang D, Li L, Lin HW	Int J Syst Evol Microbiol	10.1099/ijsem.0.005506	2022	*

References

#11000

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43383

#18490

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#39301 ; Curators of the CIP;
#54533 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38891

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#85955

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40471.1 )

#121655 Collection of Institut Pasteur ; Curators of the CIP; CIP 105483
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Actinomadura pelletieri in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Actinomadura pelletieri DSM 43383 DSM 43383

Actinomadura pelletieri DSM 43383

Change proposal

Change proposal