Name: Actinomadura viridis Mt-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 16945

Type strain:

Species: Actinomadura viridis

Strain Designation: Mt-1, A-0325

Culture col. no.: DSM 43175, ATCC 27103, CBS 833.70, IMET 9546, JCM 3112, KCC A-0112, BCRC 13398, CIP 105478, IFO 15238, IMSNU 20019, KCTC 9290, NBRC 15238, VKM Ac-1315, CCRC 13398

Strain history: CIP <- 1998, JCM <- KCC: strain A-0325 <- H. Nonomura: strain Mt-1, Microtetraspora viridis

NCBI tax ID(s): 58110 (species)

SI-ID 38923

ATCC 27103, JCM 3112, CIP 105478, CCRC 13398, DSM 43175, IFO 15238, IMET 9546, VKM Ac-1315, CBS 833.70, KCTC 9290, NBRC 15238, BCRC 13398

Special Collections

Other strains from Actinomadura viridis

Section

Actinomadura viridis Mt-1 is a spore-forming bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura viridis**
	Full Scientific Name (LPSN)	Actinomadura viridis (Nonomura and Ohara 1971) Miyadoh et al. 1989

	Synonym	Actinomadura malachitica
	Synonym	Microtetraspora viridis

Information on morphological and physiological properties Morphology

[Ref.: #123867]	Gram stain	positive

[Ref.: #123867]	Cell shape	rod-shaped

[Ref.: #123867]	Motility	no

[Ref.: #19555]	Multicellular complex forming ability	yes
[Ref.: #19555]	Multicellular complex name	Aerial Mycelium
[Ref.: #19555]	Multicellular complex color	Bright green
[Ref.: #19555]	Medium Name (multicellularity)	ISP 2

[Ref.: #19555]	Multicellular complex forming ability	yes
[Ref.: #19555]	Multicellular complex name	Aerial Mycelium
[Ref.: #19555]	Multicellular complex color	Bright green
[Ref.: #19555]	Medium Name (multicellularity)	ISP 3

[Ref.: #19555]	Multicellular complex forming ability	yes
[Ref.: #19555]	Multicellular complex name	Aerial Mycelium
[Ref.: #19555]	Multicellular complex color	Bright green
[Ref.: #19555]	Medium Name (multicellularity)	ISP 4

[Ref.: #19555]	Multicellular complex forming ability	yes
[Ref.: #19555]	Multicellular complex name	Aerial Mycelium
[Ref.: #19555]	Multicellular complex color	White
[Ref.: #19555]	Medium Name (multicellularity)	ISP 5

[Ref.: #19555]	Multicellular complex forming ability	no
[Ref.: #19555]	Medium Name (multicellularity)	ISP 6

[Ref.: #19555]	Multicellular complex forming ability	no
[Ref.: #19555]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #10843]

Culture medium

CZAPEK PEPTONE AGAR (DSMZ Medium 83)

[Ref.: #10843]

Culture medium growth

[Ref.: #10843]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10843]

Culture medium composition

expand / minimize

[Ref.: #40171]

Culture medium

MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum

[Ref.: #40171]

Culture medium growth

[Ref.: #40171]

Culture medium composition

expand / minimize

[Ref.: #10843]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #10843]

Culture medium growth

[Ref.: #10843]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10843]

Culture medium composition

expand / minimize

[Ref.: #123867]

Culture medium

CIP Medium 236

[Ref.: #123867]

Culture medium growth

[Ref.: #123867]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #123867]

Culture medium

CIP Medium 57

[Ref.: #123867]

Culture medium growth

[Ref.: #123867]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #10843]	growth	37 °C
[Ref.: #40171]	growth	30 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #123867]	growth	5-41 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]

Oxygen tolerance

aerobe Confidence in %91.323

[Ref.: #19555]	Spore description	Formation of spore chains: short chains ("four spores"), spore surface: smooth
[Ref.: #19555]	Type of spore	spore
[Ref.: #19555]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #123867]	NaCl	no	growth	0	%
[Ref.: #123867]	NaCl	no	growth	2	%
[Ref.: #123867]	NaCl	no	growth	4	%
[Ref.: #123867]	NaCl	no	growth	6	%
[Ref.: #123867]	NaCl	no	growth	8	%
[Ref.: #123867]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: MK-9(H₆), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #123867]	esculin ChEBI	-	hydrolysis
[Ref.: #123867]	nitrate ChEBI	-	reduction
[Ref.: #123867]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #123867]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #123867]		acetoin ChEBI	-
[Ref.: #123867]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #123867]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #123867]	amylase	+
[Ref.: #123867]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #123867]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #123867]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #123867]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #123867]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #123867]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #123867]	oxidase	-
[Ref.: #123867]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #123867]	tryptophan deaminase	-
[Ref.: #123867]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #123867]	8731	-	+	-	-	-	+	+	-	-	+	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #10843]	Sample type/isolated from	soil

[Ref.: #67770]	Sample type/isolated from	Soil

[Ref.: #123867]	Sample type/isolated from	Environment, Soil
[Ref.: #123867]	Geographic location (country and/or sea, region)	Leicester
[Ref.: #123867]	Country	United Kingdom
[Ref.: #123867]	Country ISO 3 Code	GBR
[Ref.: #123867]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence D85467 (>99% sequence identity) for Actinomadura from Microbeatlas

Aquatic Samples	2
Soil Samples	33
Animal Samples
Plant Samples	6
Total Samples	41

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19555]	Biosafety level	1	Risk group (German classification)
[Ref.: #10843]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #123867]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinomadura viridis strain ATCC27103 16S ribosomal RNA gene, partial sequence	AF051385	105	GenBank ENA	58110 tax ID	*
[Ref.: #20218]	Actinomadura viridis strain ATCC27103 16S ribosomal RNA gene, partial sequence	AF051387	196	GenBank ENA	58110 tax ID	*
[Ref.: #20218]	Actinomadura viridis 16S rRNA gene, strain DSM 43175T	AJ420141	1446	GenBank ENA	58110 tax ID	*
[Ref.: #20218]	Actinomadura viridis gene for 16S ribosomal RNA, partial sequence	D85467	1470	GenBank ENA	58110 tax ID	*
[Ref.: #20218]	Actinomadura viridis 16S-23S ribosomal RNA intergenic spacer region, partial sequence	AF134108	441	GenBank ENA	58110 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinomadura viridis DSM 43175	GCA_015751755	contig	GenBank ENA	58110 tax ID	*
[Ref.: #66792]	Actinomadura viridis strain DSM 43175	58110.3	wgs	PATRIC	58110 tax ID	*
[Ref.: #66792]	Actinomadura viridis DSM 43175	2880505610	draft	JGI IMG/M	58110 tax ID	*

[Ref.: #67770]	GC-content	67 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	72 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinomadura viridis strain DSM 43175 PATRIC: 58110.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomadura viridis strain DSM 43175 PATRIC: 58110.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	87.65	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	91.05	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	88.576	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	91.323	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	94	no
[Ref.: #69480]	flagellated	motile2+ⓘ	no	87.5	no

Availability in culture collections External links

[Ref.: #10843]

Culture collection no.

DSM 43175, ATCC 27103, CBS 833.70, IMET 9546, JCM 3112, KCC A-0112, BCRC 13398, CIP 105478, IFO 15238, IMSNU 20019, KCTC 9290, NBRC 15238, VKM Ac-1315, CCRC 13398

[Ref.: #85950]

SI-ID 38923

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actinomadura jiaoheensis sp. nov. and Actinomadura sporangiiformans sp. nov., two novel actinomycetes isolated from muddy soil and emended description of the genus Actinomadura.	Zhao J, Guo L, Sun P, Han C, Bai L, Liu C, Li Y, Xiang W, Wang X	Antonie Van Leeuwenhoek	10.1007/s10482-015-0585-1	2015	*
Genetics	Cell wall teichoic acids of Actinomadura viridis VKM Ac-1315T.	Shashkov AS, Potekhina NV, Naumova IB, Evtushenko LI, Widmalm G	Eur J Biochem	10.1046/j.1432-1327.1999.00412.x	1999	*

References

#10843

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43175

#19555

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40171 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#85950

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID38923.1 )

#123867 Collection of Institut Pasteur ; Curators of the CIP; CIP 105478
* These data were automatically processed and therefore are not curated

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Actinomadura viridis strain DSM 43175

Actinomadura viridis strain DSM 43175

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