Actinomadura viridis (DSM 43175, ATCC 27103, CBS 833.70)

Name: Actinomadura viridis (DSM 43175, ATCC 27103, CBS 833.70)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16945

Type strain

Strain Designation Mt-1, A-0325

Culture col. no. DSM 43175 ATCC 27103 CBS 833.70 IMET 9546 JCM 3112 11 more

NCBI tax ID(s) 58110

Special Collections DSMZ JCM KCTC CIP Wink compendium

History <- KCC <- H. Nonomura, FYU, Mt-1 KCC A-0112 <-- H. Nonomura Mt-1. CIP <- 1998, JCM <- KCC: strain A-0325 <- H. Nonomura: strain Mt-1, Microtetraspora viridis

Links

Actinomadura viridis Mt-1 is a spore-forming, Gram-positive, rod-shaped bacterium that builds an aerial mycelium and was isolated from soil.

spore-forming Gram-positive rod-shaped genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 38923

ATCC 27103,JCM 3112,CIP 105478,CCRC 13398,DSM 43175,IFO 15238,IMET 9546,VKM Ac-1315,CBS 833.70,KCTC 9290,NBRC 15238,BCRC 13398

@ref 20215

Last LPSN update 2026-02-09 11:22:05

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Thermomonosporaceae

Genus Actinomadura

Species Actinomadura viridis

Full scientific name Actinomadura viridis (Nonomura and Ohara 1971) Miyadoh et al. 1989

Synonyms (2)

Microtetraspora viridis
Actinomadura malachitica

BacDive ID	Other strains from **Actinomadura viridis** (1)	Type strain
16946	A. viridis DSM 43462, ATCC 27888, IMET 9581, INA 1920, ...

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
125439	positive			99.6
123867	positive	rod-shaped

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19555	Aerial Mycelium	Bright green	ISP 2
19555	Aerial Mycelium	Bright green	ISP 3
19555	Aerial Mycelium	Bright green	ISP 4
19555	Aerial Mycelium	White	ISP 5
19555			ISP 6
19555			ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
10843	CZAPEK PEPTONE AGAR (DSMZ Medium 83)	Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water
40171	MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum		Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
10843	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
123867	CIP Medium 236	Medium recipe at CIP
123867	CIP Medium 57	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
10843	positive	growth	37
40171	positive	growth	30
67770	positive	growth	28
123867	positive	growth	5-41

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	91.323
125439	obligate aerobe	97.3

Spore formation

@ref	Spore description	Type of spore	Spore formation
19555	Formation of spore chains: short chains ("four spores"), spore surface: smooth	spore

Halophily

@ref	Salt	Tested relation	Concentration
123867	NaCl	growth	0 %
123867	NaCl	growth	2 %
123867	NaCl	growth	4 %
123867	NaCl	growth	6 %
123867	NaCl	growth	8 %
123867	NaCl	growth	10 %

Observation

67770

Observationquinones: MK-9(H₆), MK-9(H₄)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123867	4853 ChEBI	esculin	-	hydrolysis
123867	17632 ChEBI	nitrate	-	reduction
123867	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123867	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123867	15688 ChEBI	acetoin	-
123867	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123867	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123867	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
123867	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123867	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
123867	gamma-glutamyltransferase	-	2.3.2.2
123867	gelatinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
123867	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123867	ornithine decarboxylase	-	4.1.1.17
123867	oxidase	-
123867	phenylalanine ammonia-lyase	-	4.3.1.24
68382	trypsin	-	3.4.21.4	from API zym
123867	tryptophan deaminase	-
123867	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123867	-	+	-	-	-	+	+	-	-	+	+	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
10843	soil
67770	Soil
123867	Environment, Soil	Leicester	United Kingdom	GBR	Europe

Taxonmaps

69479

Global distribution of 16S sequence D85467 (>99% sequence identity) for Actinomadura from Microbeatlas

Aquatic Samples	2
Soil Samples	33
Animal Samples
Plant Samples	6
Total Samples	41

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
10843	1	Risk group (German classification) According to TRBA 466
19555	1	Risk group (German classification) According to TRBA 466
123867	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1575175v1 assembly for Actinomadura viridis DSM 43175	contig	GCA_015751755	58110.3	2880505610	58110	73.73
124043	ASM3954027v1 assembly for Actinomadura viridis JCM 3112	scaffold	GCA_039540275			58110	46.47

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Actinomadura viridis strain ATCC27103 16S ribosomal RNA gene, partial sequence	AF051385	105	58110
20218	Actinomadura viridis strain ATCC27103 16S ribosomal RNA gene, partial sequence	AF051387	196	58110
20218	Actinomadura viridis 16S rRNA gene, strain DSM 43175T	AJ420141	1446	58110
20218	Actinomadura viridis gene for 16S ribosomal RNA, partial sequence	D85467	1470	58110
20218	Actinomadura viridis 16S-23S ribosomal RNA intergenic spacer region, partial sequence	AF134108	441	58110

GC content

@ref	GC-content (mol%)	Method
67770	67	thermal denaturation, midpoint method (Tm)
67770	72	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actinomadura viridis DSM 43175
NCBI: GCA_015751755

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	87.70	no
125439	motility	BacteriaNetⓘ	no	88.40	no
125439	gram_stain	BacteriaNetⓘ	positive	99.60	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actinomadura viridis strain DSM 43175
PATRIC: 58110.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.65	no
125438	anaerobic	anaerobicⓘ	no	91.05	no
125438	spore-forming	spore-formingⓘ	yes	88.58	no
125438	aerobic	aerobicⓘ	yes	91.32	no
125438	thermophilic	thermophileⓘ	no	94.00	no
125438	flagellated	motile2+ⓘ	no	87.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Characterization of a novel aromatic substrate-processing microcompartment in Actinobacteria.	Doron L, Sutter M, Kerfeld CA.	mBio	10.1128/mbio.01216-23	2023
Enzymology	Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors.	Heuer H, Kroppenstedt RM, Lottmann J, Berg G, Smalla K.	Appl Environ Microbiol	10.1128/aem.68.3.1325-1335.2002	2002
Genetics	Cell wall teichoic acids of Actinomadura viridis VKM Ac-1315T.	Shashkov AS, Potekhina NV, Naumova IB, Evtushenko LI, Widmalm G	Eur J Biochem	10.1046/j.1432-1327.1999.00412.x	1999
Phylogeny	Actinomadura jiaoheensis sp. nov. and Actinomadura sporangiiformans sp. nov., two novel actinomycetes isolated from muddy soil and emended description of the genus Actinomadura.	Zhao J, Guo L, Sun P, Han C, Bai L, Liu C, Li Y, Xiang W, Wang X	Antonie Van Leeuwenhoek	10.1007/s10482-015-0585-1	2015

References

#10843	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43175
#19555	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40171	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123867	Collection of Institut Pasteur ; Curators of the CIP; CIP 105478
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Actinomadura viridis (DSM 43175, ATCC 27103, CBS 833.70)

Name and taxonomic classification

Morphology

Cell morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_015751755

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media