Name: Actinomadura livida DSM 43677
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16930

Type strain:

Species: Actinomadura livida

Culture col. no.: DSM 43677, ATCC 33578, INA 1678, JCM 3387, CPCC 203281, IFO 14682, IMET 9575, IMSNU 20008, IMSNU 22191, MTCC 1382, NBRC 14682, VKM Ac-908

Strain history: DSM 43677 <-- N. S. Agre.

NCBI tax ID(s): 79909 (species)

SI-ID 45959

ATCC 33578, DSM 43677, JCM 3387, IFO 14682, IMET 9575, VKM Ac-908, KCTC 9556, NBRC 14682

Section

Actinomadura livida DSM 43677 is a spore-forming, mesophilic, Gram-positive bacterium of the family Thermomonosporaceae.

Gram-positive
spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Thermomonosporaceae*
	Genus	*Actinomadura*
	Species	**Actinomadura livida**
	Full Scientific Name (LPSN)	Actinomadura livida Lavrova and Preobrazhenskaya 1975 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #19578]	Incubation period	10-14 days
[Ref.: #19578]	Cultivation medium used	ISP 2

[Ref.: #19578]	Incubation period	10-14 days
[Ref.: #19578]	Cultivation medium used	ISP 3

[Ref.: #19578]	Incubation period	10-14 days
[Ref.: #19578]	Cultivation medium used	ISP 4

[Ref.: #19578]	Incubation period	10-14 days
[Ref.: #19578]	Cultivation medium used	ISP 5

[Ref.: #19578]	Incubation period	10-14 days
[Ref.: #19578]	Cultivation medium used	ISP 6

[Ref.: #19578]	Incubation period	10-14 days
[Ref.: #19578]	Cultivation medium used	ISP 7

[Ref.: #19578]	Multicellular complex forming ability	no
[Ref.: #19578]	Medium Name (multicellularity)	ISP 2

[Ref.: #19578]	Multicellular complex forming ability	no
[Ref.: #19578]	Medium Name (multicellularity)	ISP 3

[Ref.: #19578]	Multicellular complex forming ability	no
[Ref.: #19578]	Medium Name (multicellularity)	ISP 4

[Ref.: #19578]	Multicellular complex forming ability	no
[Ref.: #19578]	Medium Name (multicellularity)	ISP 5

[Ref.: #19578]	Multicellular complex forming ability	no
[Ref.: #19578]	Medium Name (multicellularity)	ISP 6

[Ref.: #19578]	Multicellular complex forming ability	no
[Ref.: #19578]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #11240]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #11240]

Culture medium growth

[Ref.: #11240]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #11240]

Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

[Ref.: #11240]

Culture medium growth

[Ref.: #11240]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #19578]

Culture medium

ISP 2

[Ref.: #19578]

Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

ISP 3

[Ref.: #19578]

Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

ISP 4

[Ref.: #19578]

Culture medium growth

[Ref.: #19578]

Culture medium composition

expand / minimize

[Ref.: #19578]

Culture medium

ISP 5

[Ref.: #19578]

Culture medium growth

[Ref.: #19578]

Culture medium composition

expand / minimize

[Ref.: #19578]

Culture medium

ISP 6

[Ref.: #19578]

Culture medium growth

[Ref.: #19578]

Culture medium composition

expand / minimize

[Ref.: #19578]

Culture medium

ISP 7

[Ref.: #19578]

Culture medium growth

[Ref.: #19578]

Culture medium composition

expand / minimize

[Ref.: #11240]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #11240]

Culture medium growth

[Ref.: #11240]

Culture medium link

Medium recipe at MediaDive

[Ref.: #11240]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #11240]	growth	28 °C
[Ref.: #19578]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #11240]	Temperature range	mesophilic
[Ref.: #19578]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #19578]		NaCl		maximum	5	%

[Ref.: #67770]

Observation

quinones: MK-9(H₆), MK-9(H₈), MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #19578]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #19578]	cellulose ChEBI	+
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #19578]	fructose ChEBI	+
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #19578]	glucose ChEBI	+
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #19578]	mannitol ChEBI	+
[Ref.: #19578]	myo-inositol ChEBI	+
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #19578]	raffinose ChEBI	+
[Ref.: #19578]	rhamnose ChEBI	+
[Ref.: #19578]	sucrose ChEBI	+
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #19578]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E, API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #19578]	428	-	-	-	-	-	-	-	-	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #19578]	414	not determinedn.d.	+	+	+	-	+	+	+	+	+	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence AJ293706 (>99% sequence identity) for Actinomadura from Microbeatlas

Aquatic Samples	139
Soil Samples	1327
Animal Samples	132
Plant Samples	159
Total Samples	1757

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #19578]	Biosafety level	1	Risk group (German classification)
[Ref.: #11240]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Actinomadura livida strain ATCC33578 16S ribosomal RNA gene, partial sequence	AF051382	108	ENA	79909 tax ID	*
[Ref.: #20218]	Actinomadura livida strain ATCC33578 16S ribosomal RNA gene, partial sequence	AF051388	191	ENA	79909 tax ID	*
[Ref.: #20218]	Actinomadura livida partial 16S rRNA gene, strain IMSNU 22191T	AJ293706	1525	ENA	79909 tax ID	*
[Ref.: #20218]	Actinomadura livida 16S ribosomal RNA gene, partial sequence	AF163116	1466	ENA	79909 tax ID	*
[Ref.: #20218]	Actinomadura livida 16S-23S ribosomal RNA intergenic spacer sequence	AF163127	464	ENA	79909 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinomadura livida JCM 3387	GCA_014648675	scaffold	GenBank	79909 tax ID	*
[Ref.: #66792]	Actinomadura livida strain JCM 3387	79909.3	wgs	PATRIC	79909 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinomadura livida JCM 3387 NCBI: GCA_014648675.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinomadura livida JCM 3387 NCBI: GCA_014648675.1
[Ref.: #69480]	flagellated	no	98.509	no
[Ref.: #69480]	gram-positive	yes	91.261	no
[Ref.: #69480]	anaerobic	no	98.12	no
[Ref.: #69480]	aerobic	yes	85.735	no
[Ref.: #69480]	halophile	no	81.047	no
[Ref.: #69480]	spore-forming	yes	89.614	no
[Ref.: #69480]	thermophile	no	95.959	no
[Ref.: #69480]	glucose-util	yes	88.322	yes
[Ref.: #69480]	motile	no	92.722	no
[Ref.: #69480]	glucose-ferment	no	90.71	no

Availability in culture collections External links

[Ref.: #11240]

Culture collection no.

DSM 43677, ATCC 33578, INA 1678, JCM 3387, CPCC 203281, IFO 14682, IMET 9575, IMSNU 20008, IMSNU 22191, MTCC 1382, NBRC 14682, VKM Ac-908

[Ref.: #85935]

SI-ID 45959

References

#11240

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43677

#19578

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#85935

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID45959.1 )

* These data were automatically processed and therefore are not curated

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Actinomadura livida JCM 3387

Actinomadura livida JCM 3387

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