Thermococcus alcaliphilus (DSM 10322, AEDII12)

Name: Thermococcus alcaliphilus (DSM 10322, AEDII12)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16867

Type strain

Strain Designation AEDII12

Culture col. no. DSM 10322 AEDII12

NCBI tax ID(s) 139207

Special Collections DSMZ

History DSMZ <- M. Keller and K. O. Stetter; AEDII12

Links

Thermococcus alcaliphilus AEDII12 is an anaerobe archaeon that was isolated from shallow marine hydrothermal system at Vulcano.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 101005

DSM 10322

@ref 20215

Last LPSN update 2026-05-29 08:28:52

Domain Archaea

Phylum Methanobacteriota

Class Thermococci

Order Thermococcales

Family Thermococcaceae

Genus Thermococcus

Species Thermococcus alcaliphilus

Full scientific name Thermococcus alcaliphilus Keller et al. 1997

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3906	MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)		Medium recipe at MediaDive	Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
3906	ALKALIPHILIC THERMOCOCCUS MEDIUM (DSMZ Medium 926)		Medium recipe at MediaDive	Name: ALKALIPHILIC THERMOCOCCUS MEDIUM (DSMZ Medium 926) Composition: MgCl2 x 6 H2O 2.75 g/l K2HPO4 0.5 g/l KCl 0.325 g/l NaHCO3 0.16 g/l NaBr 0.05 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l H3BO3 0.015 g/l NaCl 0.01 g/l KI 0.0075 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
3906	positive	growth	85

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
3906	anaerobe
125439	anaerobe	98.522

Spore formation

@ref	Spore formation	Confidence
125438		90.377

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Volcanic
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
3906	shallow marine hydrothermal system at Vulcano	Italy	ITA	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3906	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2405453v1 assembly for Thermococcus alcaliphilus AEDII12	contig	GCA_024054535	139207.4	8045828371	139207	74.19

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Thermococcus alcaliphilus gene for 16S rRNA, partial sequence, strain:DSM 10322	AB055121	1420		139207
20218	Thermococcus alcaliphilus 16S rRNA gene, strain DSM 10322 Type	AJ291812	1306		139207

GC content

3906

GC-content (mol%)42

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2405453v1 assembly for Thermococcus alcaliphilus AEDII12
NCBI: GCA_024054535

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	98.52	no
125439	gram_stain	BacteriaNetⓘ	variable	74.65	no
125439	motility	BacteriaNetⓘ	yes	43.65	no
125439	spore_formation	BacteriaNetⓘ	yes	46.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thermococcus alcaliphilus AEDII12
NCBI: GCA_024054535.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	69.88	no
125438	anaerobic	anaerobicⓘ	yes	87.47	yes
125438	aerobic	aerobicⓘ	no	86.99	no
125438	spore-forming	spore-formingⓘ	no	90.38	no
125438	thermophilic	thermophileⓘ	yes	83.66	yes
125438	flagellated	motile2+ⓘ	no	79.58	no

Literature

Title	Authors	Journal	DOI	Year	Topic
Thermococcus argininiproducens sp. nov., an arginine biosynthesis archaeal species isolated from the Central Indian Ocean ridge.	Park YJ, Lim JK, Kim YJ, Yang SH, Lee HS, Kang SG, Lee JH, Yang Y, Kwon KK.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005760	2023
Thermococcus alcaliphilus sp. nov., a new hyperthermophilic archaeum growing on polysulfide at alkaline pH.	Keller M, Braun FJ, Dirmeier R, Hafenbradl D, Burggraf S, Rachel R, Stetter KO	Arch Microbiol		1995	Phylogeny

References

#3906	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 10322
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thermococcus alcaliphilus (DSM 10322, AEDII12)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_024054535

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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