Archive BacDiveID:10.13145/bacdive16867.20230509.8.1

Strain identifier

BacDive ID: 16867

Type strain:

Species: Thermococcus alcaliphilus

Strain Designation: AEDII12

Strain history: <- M. Keller and K. O. Stetter; AEDII12

NCBI tax ID(s): 139207 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 3906

BacDive-ID: 16867

DSM-Number: 10322

keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Thermococcus alcaliphilus AEDII12 is an anaerobe, hyperthermophilic archaeon that was isolated from shallow marine hydrothermal system at Vulcano.

NCBI tax id

NCBI tax id: 139207
Matching level: species

strain history

@ref: 3906
history: <- M. Keller and K. O. Stetter; AEDII12

doi: 10.13145/bacdive16867.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Thermococci
order: Thermococcales
family: Thermococcaceae
genus: Thermococcus
species: Thermococcus alcaliphilus
full scientific name: Thermococcus alcaliphilus Keller et al. 1997

@ref: 3906

domain: Archaea

phylum: Euryarchaeota

class: Thermococci

order: Thermococcales

family: Thermococcaceae

genus: Thermococcus

species: Thermococcus alcaliphilus

full scientific name: Thermococcus alcaliphilus Keller et al. 1997

strain designation: AEDII12

type strain: yes

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
3906	MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760)	yes	https://mediadive.dsmz.de/medium/760	Name: MARINE THERMOCOCCUS MEDIUM (DSMZ Medium 760) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
3906	ALKALIPHILIC THERMOCOCCUS MEDIUM (DSMZ Medium 926)	yes	https://mediadive.dsmz.de/medium/926	Name: ALKALIPHILIC THERMOCOCCUS MEDIUM (DSMZ Medium 926) Composition: MgCl2 x 6 H2O 2.75 g/l K2HPO4 0.5 g/l KCl 0.325 g/l NaHCO3 0.16 g/l NaBr 0.05 g/l MgSO4 x 7 H2O 0.03 g/l Nitrilotriacetic acid 0.015 g/l H3BO3 0.015 g/l NaCl 0.01 g/l KI 0.0075 g/l MnSO4 x H2O 0.005 g/l CoSO4 x 7 H2O 0.0018 g/l ZnSO4 x 7 H2O 0.0018 g/l FeSO4 x 7 H2O 0.001 g/l CaCl2 x 2 H2O 0.001 g/l NiCl2 x 6 H2O 0.0003 g/l AlK(SO4)2 x 12 H2O 0.0002 g/l CuSO4 x 5 H2O 0.0001 g/l Na2MoO4 x 2 H2O 0.0001 g/l Na2WO4 x 2 H2O 4e-06 g/l Na2SeO3 x 5 H2O 3e-06 g/l Distilled water

culture temp

@ref: 3906
growth: positive
type: growth
temperature: 85
range: hyperthermophilic

Physiology and metabolism

oxygen tolerance

@ref: 3906
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref: 3906
sample type: shallow marine hydrothermal system at Vulcano
country: Italy
origin.country: ITA
continent: Europe

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Volcanic
#Condition	#Thermophilic (>45°C)

Safety information

risk assessment

@ref: 3906
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref	description	accession	length	database	NCBI tax ID
20218	Thermococcus alcaliphilus gene for 16S rRNA, partial sequence, strain:DSM 10322	AB055121	1420	ena	139207
20218	Thermococcus alcaliphilus 16S rRNA gene, strain DSM 10322 Type	AJ291812	1306	ena	139207

Genome sequences

@ref: 66792
description: Thermococcus alcaliphilus AEDII12
accession: GCA_024054535
assembly level: contig
database: ncbi
NCBI tax ID: 139207

GC content

@ref: 3906
GC-content: 42

Genome-based predictions

predictions

trait	prediction	confidence	training_data
flagellated	no	95.591	no
gram-positive	no	95.27	no
anaerobic	yes	98.772	yes
aerobic	no	97.569	no
halophile	no	65.457	no
spore-forming	no	96.295	no
motile	no	77.741	no
glucose-ferment	no	64.431	no
thermophile	yes	100	no
glucose-util	yes	81.506	no

External links

@ref: 3906

culture collection no.: DSM 10322

straininfo link

@ref: 85875
straininfo: 101005

literature

topic: Phylogeny
Pubmed-ID: 8588740
title: Thermococcus alcaliphilus sp. nov., a new hyperthermophilic archaeum growing on polysulfide at alkaline pH.
authors: Keller M, Braun FJ, Dirmeier R, Hafenbradl D, Burggraf S, Rachel R, Stetter KO
journal: Arch Microbiol
year: 1995
mesh: Archaea/genetics/*metabolism/ultrastructure, Base Sequence, DNA Primers/genetics, DNA, Bacterial/genetics, Freeze Fracturing, Hot Temperature, Hydrogen-Ion Concentration, Lipids/analysis, Microscopy, Electron, Molecular Sequence Data, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sulfides/*metabolism
topic2: Metabolism

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
3906	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10322)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-10322
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
85875	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID101005.1	StrainInfo: A central database for resolving microbial strain identifiers