Name: Ruminobacter amylophilus H 18
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 16635

Type strain:

Species: Ruminobacter amylophilus

Strain Designation: H 18

Culture col. no.: DSM 1361, ATCC 29744, VPI 2502 B

Strain history: <- W. Kühn <- M.P. Bryant <- P.N. Hobson, H 18. (VPI 2502 B)

NCBI tax ID(s): 867 (species)

SI-ID 39690

ATCC 29744, DSM 1361

Section

Ruminobacter amylophilus H 18 is an anaerobe, mesophilic bacterium that was isolated from sheep rumen.

anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Aeromonadales*
	Family	*Succinivibrionaceae*
	Genus	*Ruminobacter*
	Species	**Ruminobacter amylophilus**
	Full Scientific Name (LPSN)	Ruminobacter amylophilus (Hamlin and Hungate 1956) Stackebrandt and Hippe 1987

Synonym

Bacteroides amylophilus

Information on culture and growth conditions Culture and growth conditions

[Ref.: #643]

Culture medium

RUMINOBACTER AMYLOPHILUS MEDIUM (DSMZ Medium 147)

[Ref.: #643]

Culture medium growth

[Ref.: #643]

Culture medium link

Medium recipe at MediaDive

[Ref.: #643]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #643]			growth	37 °C

[Ref.: #643]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #643]

Oxygen tolerance

anaerobe

[Ref.: #643]

Name of produced compound

protease

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #68380]	raffinose ChEBI	-	fermentation	* from API rID32A
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A

	Metabolite production	Metabolite	Production
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Physiological tests	Metabolite	Indole test
[Ref.: #68380]		indole ChEBI	-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #68380]	alkaline phosphatase	-	3.1.3.1	* from API rID32A
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68380]	alpha-fucosidase	-	3.2.1.51	* from API rID32A
[Ref.: #68380]	alpha-galactosidase	-	3.2.1.22	* from API rID32A
[Ref.: #68380]	alpha-glucosidase	-	3.2.1.20	* from API rID32A
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #68380]	beta-galactosidase	-	3.2.1.23	* from API rID32A
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #68380]	beta-glucosidase	-	3.2.1.21	* from API rID32A
[Ref.: #68380]	beta-glucuronidase	-	3.2.1.31	* from API rID32A
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	histidine arylamidase	-		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #68380]	leucyl glycin arylamidase	-	3.4.11.1	* from API rID32A
[Ref.: #68380]	phenylalanine arylamidase	-		* from API rID32A
[Ref.: #68380]	proline-arylamidase	-	3.4.11.5	* from API rID32A
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	-		* from API rID32A
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #643]	12521	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	+	-	-	-	+/-	-	+/-	+	+/-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #643]	Sample type/isolated from	sheep rumen
[Ref.: #643]	Geographic location (country and/or sea, region)	Scotland
[Ref.: #643]	Country	United Kingdom
[Ref.: #643]	Country ISO 3 Code	GBR
[Ref.: #643]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Caprinae (Sheep/Goat)
#Host Body-Site	#Organ	#Rumen

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Y15992 (>99% sequence identity) for Ruminobacter amylophilus subclade from Microbeatlas

Aquatic Samples	138
Soil Samples	139
Animal Samples	8089
Plant Samples	363
Total Samples	8729

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #643]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Ruminobacter amylophilus 16S rRNA gene	Y15992	1539	ENA	867 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ruminobacter amylophilus DSM 1361	GCA_900115655	scaffold	GenBank	867 tax ID	*
[Ref.: #66792]	Ruminobacter amylophilus strain DSM 1361	867.3	wgs	PATRIC	867 tax ID	*
[Ref.: #66792]	Ruminobacter amylophilus DSM 1361	2595698241	draft	JGI IMG/M	867 tax ID	*

[Ref.: #643]

GC-content

42.1 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ruminobacter amylophilus DSM 1361 NCBI: GCA_900115655.1
[Ref.: #69480]	motile	no	83.197	no
[Ref.: #69480]	flagellated	no	92.968	no
[Ref.: #69480]	gram-positive	no	96.67	no
[Ref.: #69480]	anaerobic	yes	96.097	yes
[Ref.: #69480]	aerobic	no	96.101	yes
[Ref.: #69480]	halophile	no	73.035	no
[Ref.: #69480]	spore-forming	no	96.524	no
[Ref.: #69480]	glucose-ferment	yes	54.353	no
[Ref.: #69480]	thermophile	no	98.601	yes
[Ref.: #69480]	glucose-util	yes	59.825	no

Availability in culture collections External links

[Ref.: #643]

Culture collection no.

DSM 1361, ATCC 29744, VPI 2502 B

[Ref.: #85656]

SI-ID 39690

References

#643	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1361

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68380

Automatically annotated from API rID32A .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#85656

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID39690.1 )

* These data were automatically processed and therefore are not curated

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Ruminobacter amylophilus DSM 1361

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