Winkia neuii subsp. anitrata (DSM 8577, CCUG 32253, 50/90)

Name: Winkia neuii subsp. anitrata (DSM 8577, CCUG 32253, 50/90)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 166

Type strain

Strain Designation 50/90

Culture col. no. DSM 8577 CCUG 32253 50/90 CIP 104016

NCBI tax ID(s) 29318

Special Collections DSMZ CCUG Literature strains CIP

Links

Winkia neuii subsp. anitrata 50/90 is an anaerobe, mesophilic, Gram-positive prokaryote that was isolated from blood culture.

Gram-positive rod-shaped anaerobe mesophilic 16S sequence

Name and taxonomic classification

SI-ID 7885

LMG 14788,CCUG 32253,CIP 104016,DSM 8577,ATCC 51849,CCM 4569

@ref 20215

Last LPSN update 2025-12-16 09:46:47

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Actinomycetales

Family Actinomycetaceae

Genus Winkia

Species Winkia neuii subsp. anitrata

Full scientific name Winkia neuii subsp. anitrata (Funke et al. 1994) Nouioui et al. 2018

Synonyms (1)

Actinomyces neuii subsp. anitratus

BacDive ID	Other strains from **Winkia neuii subsp. anitrata** (22)	Type strain
135360	W. neuii subsp. anitrata 219/92, CIP 104018, ATCC 700050
144652	W. neuii subsp. anitrata CCUG 26086
144871	W. neuii subsp. anitrata CCUG 26929
146870	W. neuii subsp. anitrata CCUG 32337
150575	W. neuii subsp. anitrata CCUG 41640
150728	W. neuii subsp. anitrata CCUG 42098
150972	W. neuii subsp. anitrata CCUG 42913
151317	W. neuii subsp. anitrata CCUG 43734
152293	W. neuii subsp. anitrata CCUG 46366
152881	W. neuii subsp. anitrata CCUG 47863
153392	W. neuii subsp. anitrata CCUG 49426
153487	W. neuii subsp. anitrata CCUG 49728
153571	W. neuii subsp. anitrata CCUG 50221
153745	W. neuii subsp. anitrata CCUG 50850
153854	W. neuii subsp. anitrata CCUG 51375
154174	W. neuii subsp. anitrata CCUG 52662
154266	W. neuii subsp. anitrata CCUG 52928
155426	W. neuii subsp. anitrata CCUG 57708
155570	W. neuii subsp. anitrata CCUG 58185
155702	W. neuii subsp. anitrata CCUG 59054
155841	W. neuii subsp. anitrata CCUG 59461
155937	W. neuii subsp. anitrata CCUG 59688

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121280	positive	rod-shaped

Colony morphology

@ref	Incubation period
50932	2 days
121280

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3308	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23109	Columbia agar
41361	MEDIUM 6 - Columbia agar with 10 % horse blood		Distilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121280	CIP Medium 6	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3308	positive	growth	37	mesophilic
23109		growth	37	mesophilic
41361	positive	growth	37	mesophilic
50932	positive	growth	37	mesophilic
121280	positive	growth	30-41
121280	negative	growth	10
121280	negative	growth	25
121280	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
50932	anaerobe
50932	microaerophile

Halophily

@ref	Salt	Growth	Tested relation	Concentration
121280	NaCl	positive	growth	0-6 %
121280	NaCl		growth	8 %
121280	NaCl		growth	10 %

Murein

@ref	Murein short key	Type
3308	A11.51	A5alpha L-Lys-L-Lys-D-Glu

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23109	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
23109	17426 ChEBI	5-dehydro-D-gluconate	+	builds acid from
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
23109	17057 ChEBI	cellobiose	+/-	builds acid from
121280	16947 ChEBI	citrate	-	carbon source
23109	17108 ChEBI	D-arabinose	-	builds acid from
23109	18333 ChEBI	D-arabitol	+	builds acid from
23109	15824 ChEBI	D-fructose	+	builds acid from
23109	28847 ChEBI	D-fucose	+/-	builds acid from
23109	12936 ChEBI	D-galactose	+	builds acid from
23109	17634 ChEBI	D-glucose	+	builds acid from
23109	62318 ChEBI	D-lyxose	+	builds acid from
23109	16899 ChEBI	D-mannitol	+	builds acid from
23109	16024 ChEBI	D-mannose	+	builds acid from
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
23109	16988 ChEBI	D-ribose	+	builds acid from
23109	17924 ChEBI	D-sorbitol	-	builds acid from
23109	16443 ChEBI	D-tagatose	+/-	builds acid from
23109	65327 ChEBI	D-xylose	+	builds acid from
23109	17113 ChEBI	erythritol	+	builds acid from
23109	4853 ChEBI	esculin	-	hydrolysis
121280	4853 ChEBI	esculin	+	hydrolysis
23109	16813 ChEBI	galactitol	+/-	builds acid from
23109	5291 ChEBI	gelatin	-	hydrolysis
23109	28066 ChEBI	gentiobiose	+/-	builds acid from
23109	24265 ChEBI	gluconate	+/-	builds acid from
23109	17754 ChEBI	glycerol	+	builds acid from
23109	28087 ChEBI	glycogen	+/-	builds acid from
121280	606565 ChEBI	hippurate	+	hydrolysis
23109	15443 ChEBI	inulin	-	builds acid from
23109	30849 ChEBI	L-arabinose	-	builds acid from
23109	18403 ChEBI	L-arabitol	+/-	builds acid from
23109	18287 ChEBI	L-fucose	+/-	builds acid from
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
23109	62345 ChEBI	L-rhamnose	-	builds acid from
23109	17266 ChEBI	L-sorbose	-	builds acid from
23109	65328 ChEBI	L-xylose	-	builds acid from
23109	17716 ChEBI	lactose	+	builds acid from
23109	17306 ChEBI	maltose	+	builds acid from
23109	6731 ChEBI	melezitose	+	builds acid from
23109	28053 ChEBI	melibiose	+	builds acid from
23109	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
23109	37657 ChEBI	methyl D-glucoside	+	builds acid from
23109	17268 ChEBI	myo-inositol	+	builds acid from
23109	506227 ChEBI	N-acetylglucosamine	+/-	builds acid from
23109	17632 ChEBI	nitrate	-	reduction
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
121280	17632 ChEBI	nitrate	-	reduction
121280	17632 ChEBI	nitrate	+	respiration
121280	16301 ChEBI	nitrite	-	reduction
23109	16634 ChEBI	raffinose	+	builds acid from
68380	16634 ChEBI	raffinose	+	fermentation	from API rID32A
23109	26546 ChEBI	rhamnose	+	builds acid from
23109	15963 ChEBI	ribitol	+	builds acid from
23109	17814 ChEBI	salicin	+/-	builds acid from
23109	17992 ChEBI	sucrose	+	builds acid from
23109	27082 ChEBI	trehalose	+	builds acid from
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
23109	16199 ChEBI	urea	-	hydrolysis
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A
23109	17151 ChEBI	xylitol	-	builds acid from

Antibiotic resistance

@ref	Metabolite	Is antibiotic	Is sensitive	Is resistant
121280	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A
121280	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
121280	15688 ChEBI	acetoin	-
121280	17234 ChEBI	glucose
68380	35581 ChEBI	indole		from API rID32A

Enzymes

@ref	Value	Activity	Ec
23109	acid phosphatase	-	3.1.3.2
68382	acid phosphatase	-	3.1.3.2	from API zym
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
121280	alcohol dehydrogenase	-	1.1.1.1
23109	alkaline phosphatase	+/-	3.1.3.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68380	alkaline phosphatase	+	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
23109	alpha-galactosidase	+	3.2.1.22
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	+	3.2.1.22	from API rID32A
23109	alpha-glucosidase	+	3.2.1.20
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
23109	alpha-mannosidase	+	3.2.1.24
68382	alpha-mannosidase	-	3.2.1.24	from API zym
121280	amylase	-
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
23109	beta-galactosidase	+	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
121280	beta-galactosidase	+	3.2.1.23
68380	beta-galactosidase	+	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
23109	beta-glucosidase	-	3.2.1.21
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
23109	beta-glucuronidase	-	3.2.1.31
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
121280	caseinase	+	3.4.21.50
23109	catalase	+	1.11.1.6
121280	catalase	+	1.11.1.6
23109	chymotrypsin	-	3.4.4.5
68382	cystine arylamidase	-	3.4.11.3	from API zym
121280	DNase	-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
121280	gamma-glutamyltransferase	-	2.3.2.2
121280	gelatinase	-
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	+		from API rID32A
68380	L-arginine arylamidase	+		from API rID32A
121280	lecithinase	-
23109	leucine arylamidase	+	3.4.11.1
68382	leucine arylamidase	-	3.4.11.1	from API zym
68380	leucine arylamidase	+	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	+	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
121280	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
121280	ornithine decarboxylase	+	4.1.1.17
121280	oxidase	-
121280	phenylalanine ammonia-lyase	-	4.3.1.24
68380	phenylalanine arylamidase	+		from API rID32A
68380	proline-arylamidase	+	3.4.11.5	from API rID32A
23109	pyrazinamidase	+	3.5.1.B15
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	+		from API rID32A
23109	trypsin	-	3.4.21.4
68382	trypsin	-	3.4.21.4	from API zym
121280	tryptophan deaminase	-
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
121280	tween esterase	+/-
121280	urease	-	3.5.1.5
68380	urease	-	3.5.1.5	from API rID32A
23109	valine arylamidase	-
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121280	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
3308	-	-	+	+	-	+	+	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	-	+/-	+	+	+/-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body-Site	#Other	#Abscess
#Condition	#Anoxic (anaerobic)	-
#Infection	#Patient	#Blood culture

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3308	blood culture	Zürich	Switzerland	CHE	Europe
23109	abscesses in association with mixed anaerobic flora, blood culture
50932	Human blood culture	Zürich	Switzerland	CHE	Europe
121280	Blood culture	Zurich	Switzerland	CHE	Europe

Taxonmaps

69479

Global distribution of 16S sequence AM084229 (>99% sequence identity) for Winkia neuii subclade from Microbeatlas

Aquatic Samples	890
Soil Samples	753
Animal Samples	28891
Plant Samples	247
Total Samples	30781

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3308	2	Risk group (German classification) According to TRBA 466
121280	2	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Actinomyces neuii subsp. anitratus partial 16S rRNA gene, isolate DSM 8577T	AM084229	1497		29318
23109	Actinomyces neuii subsp. anitratus 16S rRNA gene, strain 50/90	X71862	1489		29318

GC content

@ref	GC-content (mol%)	Method
3308	55	thermal denaturation, midpoint method (Tm)
23109	55.0

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018

References

#3308	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8577
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23109	GUIDO FUNKE, SIMON STUBBS, ALEXANDER VON GRAEVENITZ, MATTHEW D. COLLINS: Assignment of Human-Derived CDC Group 1 Coryneform Bacteria and CDC Group 1-Like Coryneform Bacteria to the Genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp. nov., and Actinomyces neuii subsp. anitratus subsp. nov.. IJSEM 44: 167 - 171 1994 ( DOI 10.1099/00207713-44-1-167 , PubMed 8123558 )
#41361	; Curators of the CIP;
#50932	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32253
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121280	Collection of Institut Pasteur ; Curators of the CIP; CIP 104016
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Winkia neuii subsp. anitrata (DSM 8577, CCUG 32253, 50/90)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Murein

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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