Name: Winkia neuii subsp. anitrata 50/90
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 166

Type strain:

Species: Winkia neuii subsp. anitrata

Strain Designation: 50/90

Culture col. no.: DSM 8577, CCUG 32253, CIP 104016

Strain history: CIP <- 1994, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain 50/90

NCBI tax ID(s): 29318 (subspecies)

SI-ID 7885

LMG 14788, CCUG 32253, CIP 104016, DSM 8577, ATCC 51849, CCM 4569

Special Collections

Other strains from Winkia neuii subsp. anitrata

Section

Winkia neuii subsp. anitrata 50/90 is an anaerobe, Gram-positive, rod-shaped bacterium that was isolated from blood culture.

Gram-positive
rod-shaped
anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Actinomycetales*
	Family	*Actinomycetaceae*
	Genus	*Winkia*
	Species	**Winkia neuii subsp. anitrata**
	Full Scientific Name (LPSN)	Winkia neuii subsp. anitrata (Funke et al. 1994) Nouioui et al. 2018

Synonym

Actinomyces neuii subsp. anitratus

Information on morphological and physiological properties Morphology

[Ref.: #121280]	Gram stain	positive

[Ref.: #121280]	Cell shape	rod-shaped

[Ref.: #121280]	Motility	no

[Ref.: #50932]

Incubation period

2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3308]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #3308]

Culture medium growth

[Ref.: #3308]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3308]

Culture medium composition

expand / minimize

[Ref.: #23109]

Culture medium

Columbia agar

[Ref.: #23109]

Culture medium growth

[Ref.: #41361]

Culture medium

MEDIUM 6 - Columbia agar with 10 % horse blood

[Ref.: #41361]

Culture medium growth

[Ref.: #41361]

Culture medium composition

expand / minimize

[Ref.: #121280]

Culture medium

CIP Medium 6

[Ref.: #121280]

Culture medium growth

[Ref.: #121280]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3308]		growth	37 °C
[Ref.: #23109]		growth	37 °C
[Ref.: #41361]		growth	37 °C
[Ref.: #50932]		growth	37 °C
[Ref.: #121280]		growth	30-41 °C
[Ref.: #121280]	no	growth	10 °C
[Ref.: #121280]	no	growth	25 °C
[Ref.: #121280]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #50932]	Oxygen tolerance	anaerobe
[Ref.: #50932]	Oxygen tolerance	microaerophile

	Salt	Tested relation		Salt conc.
[Ref.: #121280]	NaCl		growth	0-6	%
[Ref.: #121280]	NaCl	no	growth	8	%
[Ref.: #121280]	NaCl	no	growth	10	%

[Ref.: #3308]	Murein short key	A11.51
[Ref.: #3308]	Murein types	A5alpha L-Lys-L-Lys-D-Glu

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23109]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23109]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #68380]	arginine ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #23109]	cellobiose ChEBI	+/-	builds acid from
[Ref.: #121280]	citrate ChEBI	-	carbon source
[Ref.: #23109]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23109]	D-arabitol ChEBI	+	builds acid from
[Ref.: #23109]	D-fructose ChEBI	+	builds acid from
[Ref.: #23109]	D-fucose ChEBI	+/-	builds acid from
[Ref.: #23109]	D-galactose ChEBI	+	builds acid from
[Ref.: #23109]	D-glucose ChEBI	+	builds acid from
[Ref.: #23109]	D-lyxose ChEBI	+	builds acid from
[Ref.: #23109]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23109]	D-mannose ChEBI	+	builds acid from
[Ref.: #68380]	D-mannose ChEBI	-	fermentation	* from API rID32A
[Ref.: #23109]	D-ribose ChEBI	+	builds acid from
[Ref.: #23109]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23109]	D-tagatose ChEBI	+/-	builds acid from
[Ref.: #23109]	D-xylose ChEBI	+	builds acid from
[Ref.: #23109]	erythritol ChEBI	+	builds acid from
[Ref.: #23109]	esculin ChEBI	-	hydrolysis
[Ref.: #121280]	esculin ChEBI	+	hydrolysis
[Ref.: #23109]	galactitol ChEBI	+/-	builds acid from
[Ref.: #23109]	gelatin ChEBI	-	hydrolysis
[Ref.: #23109]	gentiobiose ChEBI	+/-	builds acid from
[Ref.: #23109]	gluconate ChEBI	+/-	builds acid from
[Ref.: #23109]	glycerol ChEBI	+	builds acid from
[Ref.: #23109]	glycogen ChEBI	+/-	builds acid from
[Ref.: #121280]	hippurate ChEBI	+	hydrolysis
[Ref.: #23109]	inulin ChEBI	-	builds acid from
[Ref.: #23109]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23109]	L-arabitol ChEBI	+/-	builds acid from
[Ref.: #23109]	L-fucose ChEBI	+/-	builds acid from
[Ref.: #68380]	L-glutamate ChEBI	-	degradation	* from API rID32A
[Ref.: #23109]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #23109]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23109]	L-xylose ChEBI	-	builds acid from
[Ref.: #23109]	lactose ChEBI	+	builds acid from
[Ref.: #23109]	maltose ChEBI	+	builds acid from
[Ref.: #23109]	melezitose ChEBI	+	builds acid from
[Ref.: #23109]	melibiose ChEBI	+	builds acid from
[Ref.: #23109]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23109]	methyl D-glucoside ChEBI	+	builds acid from
[Ref.: #23109]	myo-inositol ChEBI	+	builds acid from
[Ref.: #23109]	N-acetylglucosamine ChEBI	+/-	builds acid from
[Ref.: #23109]	nitrate ChEBI	-	reduction
[Ref.: #121280]	nitrate ChEBI	-	reduction
[Ref.: #121280]	nitrate ChEBI	+	respiration
[Ref.: #68380]	nitrate ChEBI	-	reduction	* from API rID32A
[Ref.: #121280]	nitrite ChEBI	-	reduction
[Ref.: #23109]	raffinose ChEBI	+	builds acid from
[Ref.: #68380]	raffinose ChEBI	+	fermentation	* from API rID32A
[Ref.: #23109]	rhamnose ChEBI	+	builds acid from
[Ref.: #23109]	ribitol ChEBI	+	builds acid from
[Ref.: #23109]	salicin ChEBI	+/-	builds acid from
[Ref.: #23109]	sucrose ChEBI	+	builds acid from
[Ref.: #23109]	trehalose ChEBI	+	builds acid from
[Ref.: #68380]	tryptophan ChEBI	-	energy source	* from API rID32A
[Ref.: #23109]	urea ChEBI	-	hydrolysis
[Ref.: #68380]	urea ChEBI	-	hydrolysis	* from API rID32A
[Ref.: #23109]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121280]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #121280]		indole ChEBI	no
[Ref.: #68380]		indole ChEBI	no	* from API rID32A

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #121280]	acetoin ChEBI	-
[Ref.: #121280]	glucose ChEBI		+
[Ref.: #68380]	indole ChEBI			-	* from API rID32A

	Enzyme	Enzyme activity	EC number
[Ref.: #23109]	acid phosphatase	-	3.1.3.2
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68380]	alanine arylamidase	+	3.4.11.2	* from API rID32A
[Ref.: #121280]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #23109]	alkaline phosphatase	+/-	3.1.3.1
[Ref.: #68380]	alkaline phosphatase	+	3.1.3.1	* from API rID32A
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68380]	alpha-arabinosidase	-	3.2.1.55	* from API rID32A
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym, API rID32A
[Ref.: #23109]	alpha-galactosidase	+	3.2.1.22
[Ref.: #68380]	alpha-galactosidase	+	3.2.1.22	* from API rID32A
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #23109]	alpha-glucosidase	+	3.2.1.20
[Ref.: #68380]	alpha-glucosidase	+	3.2.1.20	* from API rID32A
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #23109]	alpha-mannosidase	+	3.2.1.24
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121280]	amylase	-
[Ref.: #68380]	arginine dihydrolase	-	3.5.3.6	* from API rID32A
[Ref.: #23109]	beta-galactosidase	+	3.2.1.23
[Ref.: #121280]	beta-galactosidase	+	3.2.1.23
[Ref.: #68380]	beta-galactosidase	+	3.2.1.23	* from API rID32A
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68380]	beta-Galactosidase 6-phosphate	-		* from API rID32A
[Ref.: #23109]	beta-glucosidase	-	3.2.1.21
[Ref.: #68380]	beta-glucosidase	+	3.2.1.21	* from API rID32A
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #23109]	beta-glucuronidase	-	3.2.1.31
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API rID32A
[Ref.: #121280]	caseinase	+	3.4.21.50
[Ref.: #23109]	catalase	+	1.11.1.6
[Ref.: #121280]	catalase	+	1.11.1.6
[Ref.: #23109]	chymotrypsin	-	3.4.4.5
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121280]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121280]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #121280]	gelatinase	-
[Ref.: #68380]	glutamate decarboxylase	-	4.1.1.15	* from API rID32A
[Ref.: #68380]	glutamyl-glutamate arylamidase	-		* from API rID32A
[Ref.: #68380]	glycin arylamidase	+		* from API rID32A
[Ref.: #68380]	L-arginine arylamidase	+		* from API rID32A
[Ref.: #121280]	lecithinase	-
[Ref.: #23109]	leucine arylamidase	+	3.4.11.1
[Ref.: #68380]	leucine arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68380]	leucyl glycin arylamidase	+	3.4.11.1	* from API rID32A
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121280]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym, API rID32A
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #121280]	ornithine decarboxylase	+	4.1.1.17
[Ref.: #121280]	oxidase	-
[Ref.: #121280]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68380]	phenylalanine arylamidase	+		* from API rID32A
[Ref.: #68380]	proline-arylamidase	+	3.4.11.5	* from API rID32A
[Ref.: #23109]	pyrazinamidase	+	3.5.1.B15
[Ref.: #68380]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API rID32A
[Ref.: #68380]	serine arylamidase	+		* from API rID32A
[Ref.: #23109]	trypsin	-	3.4.21.4
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #121280]	tryptophan deaminase	-
[Ref.: #68380]	tryptophan deaminase	-	4.1.99.1	* from API rID32A
[Ref.: #121280]	tween esterase	+/-
[Ref.: #121280]	urease	-	3.5.1.5
[Ref.: #68380]	urease	-	3.5.1.5	* from API rID32A
[Ref.: #23109]	valine arylamidase	-
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® rID32A:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	alpha-Galactosidasealpha GAL	beta-Galactosidasebeta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha-Glucosidasealpha GLU	beta-Glucosidasebeta GLU	alpha-Arabinosidasealpha ARA	beta-Glucuronidasebeta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	Fermentation of D-mannoseMNE	Fermentation of raffinoseRAF	Glutamate decarboxylaseGDC	alpha-Fucosidasealpha FUC	Reduction of nitrateNIT	Indole productionIND	Alkaline phosphatasePAL	L-arginine arylamidaseArgA	Proline arylamidaseProA	Leucyl glycin arylamidaseLGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	Pyrrolidonyl arylamidasePyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
[Ref.: #3308]	12579	-	-	+	+	-	+	+	-	-	-	-	+	-	-	-	-	+	+	+	+	+	+	-	+/-	+	+	+/-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121280]	7769	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3308]	Sample type/isolated from	blood culture
[Ref.: #3308]	Geographic location (country and/or sea, region)	Zürich
[Ref.: #3308]	Country	Switzerland
[Ref.: #3308]	Country ISO 3 Code	CHE
[Ref.: #3308]	Continent	Europe

[Ref.: #23109]	Sample type/isolated from	abscesses in association with mixed anaerobic flora, blood culture

[Ref.: #50932]	Sample type/isolated from	Human blood culture
[Ref.: #50932]	Geographic location (country and/or sea, region)	Zürich
[Ref.: #50932]	Country	Switzerland
[Ref.: #50932]	Country ISO 3 Code	CHE
[Ref.: #50932]	Continent	Europe

[Ref.: #121280]	Sample type/isolated from	Blood culture
[Ref.: #121280]	Geographic location (country and/or sea, region)	Zurich
[Ref.: #121280]	Country	Switzerland
[Ref.: #121280]	Country ISO 3 Code	CHE
[Ref.: #121280]	Continent	Europe
[Ref.: #121280]	Isolation date	1990
* marker position based on {}

Isolation sources categories

#Host Body-Site	#Other	#Abscess
#Condition	#Anoxic (anaerobic)	-
#Infection	#Patient	#Blood culture

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM084229 (>99% sequence identity) for Winkia neuii subclade from Microbeatlas

Aquatic Samples	890
Soil Samples	753
Animal Samples	28891
Plant Samples	247
Total Samples	30781

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3308]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121280]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #23109]	Actinomyces neuii subsp. anitratus 16S rRNA gene, strain 50/90	X71862	1489	GenBank ENA	29318 tax ID
[Ref.: #20218]	Actinomyces neuii subsp. anitratus partial 16S rRNA gene, isolate DSM 8577T	AM084229	1497	GenBank ENA	29318 tax ID	*

[Ref.: #3308]	GC-content	55 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #23109]	GC-content	55.0 mol%

Availability in culture collections External links

[Ref.: #3308]

Culture collection no.

DSM 8577, CCUG 32253, CIP 104016

[Ref.: #69848]

SI-ID 7885

References

#3308

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8577

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23109

GUIDO FUNKE, SIMON STUBBS, ALEXANDER VON GRAEVENITZ, MATTHEW D. COLLINS: Assignment of Human-Derived CDC Group 1 Coryneform Bacteria and CDC Group 1-Like Coryneform Bacteria to the Genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp. nov., and Actinomyces neuii subsp. anitratus subsp. nov.. IJSEM 44: 167 - 171 1994 ( DOI 10.1099/00207713-44-1-167 , PubMed 8123558 )

#41361 ; Curators of the CIP;
#50932 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 32253

#68380

Automatically annotated from API rID32A .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69848

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7885.1 )

#121280 Collection of Institut Pasteur ; Curators of the CIP; CIP 104016
* These data were automatically processed and therefore are not curated

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