Strain identifier

BacDive ID: 166

Type strain: Yes

Species: Winkia neuii subsp. anitrata

Strain Designation: 50/90

Strain history: CIP <- 1994, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain 50/90

NCBI tax ID(s): 29318 (subspecies)

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General

@ref: 3308

BacDive-ID: 166

DSM-Number: 8577

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Winkia neuii subsp. anitrata 50/90 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from blood culture.

NCBI tax id

  • NCBI tax id: 29318
  • Matching level: subspecies

strain history

@refhistory
3308<- G. Funke, 97/90
413611994, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain 50/90
121280CIP <- 1994, G. Funke, Inst. Med. Mikrobiol., Zürich, Switzerland: strain 50/90

doi: 10.13145/bacdive166.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Winkia
  • species: Winkia neuii subsp. anitrata
  • full scientific name: Winkia neuii subsp. anitrata (Funke et al. 1994) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces neuii subsp. anitratus

@ref: 3308

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Winkia

species: Winkia neuii subsp. anitrata

full scientific name: Winkia neuii subsp. anitrata (Funke et al. 1994) Nouioui et al. 2018

strain designation: 50/90

type strain: yes

Morphology

cell morphology

  • @ref: 121280
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refincubation period
509322 days
121280

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3308COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23109Columbia agaryes
41361MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121280CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
3308positivegrowth37mesophilic
23109growth37mesophilic
41361positivegrowth37mesophilic
50932positivegrowth37mesophilic
121280positivegrowth30-41
121280nogrowth10psychrophilic
121280nogrowth25mesophilic
121280nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
50932anaerobe
50932microaerophile

halophily

@refsaltgrowthtested relationconcentration
121280NaClpositivegrowth0-6 %
121280NaClnogrowth8 %
121280NaClnogrowth10 %

murein

  • @ref: 3308
  • murein short key: A11.51
  • type: A5alpha L-Lys-L-Lys-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23109168082-dehydro-D-gluconate-builds acid from
2310917108D-arabinose-builds acid from
2310917924D-sorbitol-builds acid from
2310915443inulin-builds acid from
2310930849L-arabinose-builds acid from
2310962345L-rhamnose-builds acid from
2310917266L-sorbose-builds acid from
2310965328L-xylose-builds acid from
2310943943methyl alpha-D-mannoside-builds acid from
2310917151xylitol-builds acid from
231094853esculin-hydrolysis
231095291gelatin-hydrolysis
2310916199urea-hydrolysis
2310917632nitrate-reduction
23109174265-dehydro-D-gluconate+builds acid from
2310915963ribitol+builds acid from
2310918333D-arabitol+builds acid from
2310915824D-fructose+builds acid from
2310912936D-galactose+builds acid from
2310917634D-glucose+builds acid from
2310962318D-lyxose+builds acid from
2310916899D-mannitol+builds acid from
2310916024D-mannose+builds acid from
2310916988D-ribose+builds acid from
2310965327D-xylose+builds acid from
2310917113erythritol+builds acid from
2310917754glycerol+builds acid from
2310917268myo-inositol+builds acid from
2310917716lactose+builds acid from
2310917306maltose+builds acid from
231096731melezitose+builds acid from
2310928053melibiose+builds acid from
2310937657methyl D-glucoside+builds acid from
2310916634raffinose+builds acid from
2310926546rhamnose+builds acid from
2310917992sucrose+builds acid from
2310927082trehalose+builds acid from
2310917057cellobiose+/-builds acid from
2310928847D-fucose+/-builds acid from
2310916443D-tagatose+/-builds acid from
2310916813galactitol+/-builds acid from
2310928066gentiobiose+/-builds acid from
2310924265gluconate+/-builds acid from
2310928087glycogen+/-builds acid from
2310918403L-arabitol+/-builds acid from
2310918287L-fucose+/-builds acid from
23109506227N-acetylglucosamine+/-builds acid from
2310917814salicin+/-builds acid from
12128016947citrate-carbon source
1212804853esculin+hydrolysis
121280606565hippurate+hydrolysis
12128017632nitrate-reduction
12128016301nitrite-reduction
12128017632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose+fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

antibiotic resistance

  • @ref: 121280
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
12128035581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
12128015688acetoin-
12128017234glucose+

enzymes

@refvalueactivityec
23109acid phosphatase-3.1.3.2
23109alkaline phosphatase+/-3.1.3.1
23109alpha-galactosidase+3.2.1.22
23109alpha-glucosidase+3.2.1.20
23109alpha-mannosidase+3.2.1.24
23109beta-galactosidase+3.2.1.23
23109beta-glucosidase-3.2.1.21
23109beta-glucuronidase-3.2.1.31
23109catalase+1.11.1.6
23109chymotrypsin-3.4.4.5
23109leucine arylamidase+3.4.11.1
23109pyrazinamidase+3.5.1.B15
23109trypsin-3.4.21.4
23109valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase+3.4.11.1
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase+3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase+3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5
121280oxidase-
121280beta-galactosidase+3.2.1.23
121280alcohol dehydrogenase-1.1.1.1
121280gelatinase-
121280amylase-
121280DNase-
121280caseinase+3.4.21.50
121280catalase+1.11.1.6
121280tween esterase+/-
121280gamma-glutamyltransferase-2.3.2.2
121280lecithinase-
121280lysine decarboxylase-4.1.1.18
121280ornithine decarboxylase+4.1.1.17
121280phenylalanine ammonia-lyase-4.3.1.24
121280tryptophan deaminase-
121280urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121280--++----------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
3308--++-++----+----++++++-+/-+++/--+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
3308blood cultureZürichSwitzerlandCHEEurope
23109abscesses in association with mixed anaerobic flora, blood culture
50932Human blood cultureZürichSwitzerlandCHEEurope
121280Blood cultureZurichSwitzerlandCHEEurope1990

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Abscess
#Condition#Anoxic (anaerobic)
#Infection#Patient#Blood culture

taxonmaps

  • @ref: 69479
  • File name: preview.99_2565.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16309;96_1377;97_1637;98_1988;99_2565&stattab=map
  • Last taxonomy: Winkia neuii subclade
  • 16S sequence: AM084229
  • Sequence Identity:
  • Total samples: 30781
  • soil counts: 753
  • aquatic counts: 890
  • animal counts: 28891
  • plant counts: 247

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
33082Risk group (German classification)
1212802Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Actinomyces neuii subsp. anitratus partial 16S rRNA gene, isolate DSM 8577TAM0842291497ena29318
23109Actinomyces neuii subsp. anitratus 16S rRNA gene, strain 50/90X718621489nuccore29318

GC content

@refGC-contentmethod
330855thermal denaturation, midpoint method (Tm)
2310955.0

External links

@ref: 3308

culture collection no.: DSM 8577, CCUG 32253, CIP 104016

straininfo link

  • @ref: 69848
  • straininfo: 7885

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3308Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 8577)https://www.dsmz.de/collection/catalogue/details/culture/DSM-8577
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
23109GUIDO FUNKE, SIMON STUBBS, ALEXANDER VON GRAEVENITZ, MATTHEW D. COLLINS10.1099/00207713-44-1-167Assignment of Human-Derived CDC Group 1 Coryneform Bacteria and CDC Group 1-Like Coryneform Bacteria to the Genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp. nov., and Actinomyces neuii subsp. anitratus subsp. nov.IJSEM 44: 167-171 19948123558
41361Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15877
50932Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 32253)https://www.ccug.se/strain?id=32253
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69848Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID7885.1StrainInfo: A central database for resolving microbial strain identifiers
121280Curators of the CIPCollection of Institut Pasteur (CIP 104016)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104016