Microbispora rosea subsp. rosea (DSM 43839, ATCC 12950, CBS 189.57)

Cell morphology

Colony morphology

Multimedia

• @ref:	11323
  multimedia content:	DSM_43839.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43839.jpg
  caption:	Medium 553 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
11323	GPHF-MEDIUM (DSMZ Medium 553)		Medium recipe at MediaDive	Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water
19964	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19964	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19964	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19964	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
19964	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19964	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
11323	N-Z-AMINE-MEDIUM (DSMZ Medium 554)		Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water

Culture temp

Oxygen tolerance

Halophily

Observation

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
19964	22599 ChEBI	arabinose	+
68368	29016 ChEBI	arginine	-	from API 20E
19964	62968 ChEBI	cellulose	+
68368	16947 ChEBI	citrate	-	from API 20E
19964	28757 ChEBI	fructose	+
68368	5291 ChEBI	gelatin	-	from API 20E
19964	17234 ChEBI	glucose	+
68368	25094 ChEBI	lysine	-	from API 20E
19964	29864 ChEBI	mannitol	+
19964	17268 ChEBI	myo-inositol	+
68368	18257 ChEBI	ornithine	-	from API 20E
19964	16634 ChEBI	raffinose	+
19964	26546 ChEBI	rhamnose	+
19964	17992 ChEBI	sucrose	+
68368	27897 ChEBI	tryptophan	-	from API 20E
68368	16199 ChEBI	urea	-	from API 20E
19964	18222 ChEBI	xylose	+

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68368	15688 ChEBI	acetoin	-	from API 20E
68368	35581 ChEBI	indole		from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68368	beta-galactosidase	+	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	-		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions	external links
66794	molybdenum cofactor biosynthesis	100	9 of 9
66794	resorcinol degradation	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	ceramide biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	enterobactin biosynthesis	100	3 of 3
66794	starch degradation	100	10 of 10
66794	biotin biosynthesis	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	gallate degradation	100	5 of 5
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	adipate degradation	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	sulfopterin metabolism	100	4 of 4
66794	hydrogen production	100	5 of 5
66794	vitamin K metabolism	100	5 of 5
66794	cellulose degradation	100	5 of 5
66794	3-chlorocatechol degradation	100	5 of 5
66794	acetate fermentation	100	4 of 4
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	palmitate biosynthesis	100	22 of 22
66794	cardiolipin biosynthesis	100	7 of 7
66794	coenzyme A metabolism	100	4 of 4
66794	aerobactin biosynthesis	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	Entner Doudoroff pathway	100	10 of 10
66794	methylglyoxal degradation	100	5 of 5
66794	flavin biosynthesis	93.33	14 of 15
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	degradation of aromatic, nitrogen containing compounds	91.67	11 of 12
66794	pentose phosphate pathway	90.91	10 of 11
66794	phenol degradation	90	18 of 20
66794	4-hydroxyphenylacetate degradation	90	9 of 10
66794	threonine metabolism	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	dTDPLrhamnose biosynthesis	87.5	7 of 8
66794	isoleucine metabolism	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	photosynthesis	85.71	12 of 14
66794	heme metabolism	85.71	12 of 14
66794	glutamate and glutamine metabolism	85.71	24 of 28
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	glutathione metabolism	85.71	12 of 14
66794	urea cycle	84.62	11 of 13
66794	tryptophan metabolism	84.21	32 of 38
66794	glycolate and glyoxylate degradation	83.33	5 of 6
66794	NAD metabolism	83.33	15 of 18
66794	histidine metabolism	82.76	24 of 29
66794	glycolysis	82.35	14 of 17
66794	metabolism of disaccharids	81.82	9 of 11
66794	purine metabolism	80.85	76 of 94
66794	myo-inositol biosynthesis	80	8 of 10
66794	factor 420 biosynthesis	80	4 of 5
66794	glycogen metabolism	80	4 of 5
66794	propionate fermentation	80	8 of 10
66794	phenylacetate degradation (aerobic)	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	vitamin B12 metabolism	79.41	27 of 34
66794	citric acid cycle	78.57	11 of 14
66794	degradation of pentoses	78.57	22 of 28
66794	allantoin degradation	77.78	7 of 9
66794	d-mannose degradation	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	leucine metabolism	76.92	10 of 13
66794	non-pathway related	76.32	29 of 38
66794	degradation of sugar acids	76	19 of 25
66794	alanine metabolism	75.86	22 of 29
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	butanoate fermentation	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	toluene degradation	75	3 of 4
66794	cyclohexanol degradation	75	3 of 4
66794	arginine metabolism	75	18 of 24
66794	oxidative phosphorylation	74.73	68 of 91
66794	lipid metabolism	74.19	23 of 31
66794	3-phenylpropionate degradation	73.33	11 of 15
66794	cholesterol biosynthesis	72.73	8 of 11
66794	propanol degradation	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	tyrosine metabolism	71.43	10 of 14
66794	pyrimidine metabolism	71.11	32 of 45
66794	sulfate reduction	69.23	9 of 13
66794	isoprenoid biosynthesis	69.23	18 of 26
66794	androgen and estrogen metabolism	68.75	11 of 16
66794	methane metabolism	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	degradation of hexoses	66.67	12 of 18
66794	octane oxidation	66.67	2 of 3
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	IAA biosynthesis	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	methionine metabolism	65.38	17 of 26
66794	proline metabolism	63.64	7 of 11
66794	d-xylose degradation	63.64	7 of 11
66794	degradation of sugar alcohols	62.5	10 of 16
66794	ketogluconate metabolism	62.5	5 of 8
66794	lysine metabolism	61.9	26 of 42
66794	phosphatidylethanolamine bioynthesis	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	polyamine pathway	60.87	14 of 23
66794	D-cycloserine biosynthesis	60	3 of 5
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	carotenoid biosynthesis	59.09	13 of 22
66794	aclacinomycin biosynthesis	57.14	4 of 7
66794	nitrate assimilation	55.56	5 of 9
66794	CMP-KDO biosynthesis	50	2 of 4
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	ribulose monophosphate pathway	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	glycine metabolism	50	5 of 10
66794	ethanol fermentation	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	grixazone biosynthesis	50	1 of 2
66794	catecholamine biosynthesis	50	2 of 4
66794	bile acid biosynthesis, neutral pathway	47.06	8 of 17
66794	ascorbate metabolism	45.45	10 of 22
66794	lipid A biosynthesis	44.44	4 of 9
66794	arachidonic acid metabolism	44.44	8 of 18
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	coenzyme M biosynthesis	40	4 of 10
66794	bacilysin biosynthesis	40	2 of 5
66794	lipoate biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	glycine betaine biosynthesis	40	2 of 5
66794	chlorophyll metabolism	38.89	7 of 18
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	carnitine metabolism	37.5	3 of 8
66794	vitamin B6 metabolism	36.36	4 of 11
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	pantothenate biosynthesis	33.33	2 of 6
66794	sphingosine metabolism	33.33	2 of 6
66794	sulfoquinovose degradation	33.33	1 of 3
66794	methanogenesis from CO2	33.33	4 of 12
66794	mevalonate metabolism	28.57	2 of 7
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	daunorubicin biosynthesis	22.22	2 of 9

API 20E

API zym

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	IMG-taxon 2681812892 annotated assembly for Microbispora rosea ATCC 12950	scaffold	GCA_900156315	58117.3	2681812892	58117	52.44
66792	ASM1686305v1 assembly for Microbispora rosea subsp. rosea NBRC 14044	contig	GCA_016863055	58118.5	8117694810	58118	43.69

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Microbispora rosea strain ATCC12950 16S ribosomal RNA gene, partial sequence	AF051394	192		58117
20218	Microbispora rosea gene for 16S ribosomal RNA, partial sequence	D86936	1473		58117

GC content

Predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Phylogenetic and Functional Characterization of Culturable Endophytic Actinobacteria Associated With Camellia spp. for Growth Promotion in Commercial Tea Cultivars.	Borah A, Thakur D.	Front Microbiol	10.3389/fmicb.2020.00318	2020
	Transduction of plasmid DNA in Streptomyces spp. and related genera by bacteriophage FP43.	McHenney MA, Baltz RH.	J Bacteriol	10.1128/jb.170.5.2276-2282.1988	1988
	CHEMICAL COMPOSITION OF CELL-WALL PREPARATIONS FROM STRAINS OF VARIOUS FORM-GENERA OF AEROBIC ACTINOMYCETES.	BECKER B, LECHEVALIER MP, LECHEVALIER HA.	Appl Microbiol	10.1128/am.13.2.236-243.1965	1965
Phylogeny	A taxonomic review of the genus Microbispora and a proposal to transfer two species to the genus Actinomadura and to combine ten species into Microbispora rosea.	Miyadoh S, Amano S, Tohyama H, Shomura T	J Gen Microbiol	10.1099/00221287-136-9-1905	1990
Phylogeny	Microbispora maris sp. nov., a novel actinobacterium isolated from the gill of the leopard coral grouper (Plectropomus leopardus).	Xie H, Xie QY, Ma QY, Yang L, Dai HF, Zhao YX, Hao YE.	Int J Syst Evol Microbiol	10.1099/ijsem.0.006568	2024
Phylogeny	Microbispora oryzae sp. nov., isolated from leaves of rice plant (Oryza sativa L.).	Muangham S, Duangmal K	J Antibiot (Tokyo)	10.1038/s41429-021-00470-x	2021
Phylogeny	Microbispora cellulosiformans sp. nov., a novel actinomycete with cellulase activity isolated from soil in the cold region.	Gong X, Xiang W, Cao X, Yu Y, Hao Y, Li L, Wang Q, Zou H, Qian C	Antonie Van Leeuwenhoek	10.1007/s10482-020-01477-4	2020
Phylogeny	Microbispora fusca sp. nov., a novel actinomycete isolated from the ear of wheat (Triticum aestivum L.).	Zhao J, Yu B, Han C, Cao P, Yu Z, Ju H, Xiang W, Wang X	Int J Syst Evol Microbiol	10.1099/ijsem.0.003725	2020
Phylogeny	Microbispora tritici sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.).	Han C, Zhao J, Yu B, Shi H, Zhang C, Guo X, Xiang W, Wang X	Antonie Van Leeuwenhoek	10.1007/s10482-019-01246-y	2019
Phylogeny	Microbispora camponoti sp. nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr.	Han C, Liu C, Zhao J, Guo L, Lu C, Li J, Jia F, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-015-0625-x	2015
Phylogeny	Microbispora bryophytorum sp. nov., an actinomycete isolated from moss (Bryophyta).	Li C, Zhang Y, Liu C, Wang H, Zhao J, Li L, Zhang Z, Wang X, Xiang W	Int J Syst Evol Microbiol	10.1099/ijs.0.000095	2015