Acrocarpospora macrocephala (DSM 44705, IFO 16266, JCM 10982)

Name: Acrocarpospora macrocephala (DSM 44705, IFO 16266, JCM 10982)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16505

Type strain

Strain Designation R-55

Culture col. no. DSM 44705 IFO 16266 JCM 10982 NBRC 16266 R-55 1 more

NCBI tax ID(s) 150177

Special Collections DSMZ JCM Wink compendium

History DSMZ <- NBRC <- S. Suzuki, Tanabe Seiyaku Co.; R-55 JCM <-- IFO 16266 <-- T. Tamura <-- S. Suzuki R-55.

Links

Acrocarpospora macrocephala R-55 is a bacterium that was isolated from soil.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 62209

IFO 16266,JCM 10982,DSM 44705,NBRC 16266,BCRC 16396,CCRC 16396

@ref 20215

Last LPSN update 2026-05-29 08:28:47

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Streptosporangiales

Family Streptosporangiaceae

Genus Acrocarpospora

Species Acrocarpospora macrocephala

Full scientific name Acrocarpospora macrocephala Tamura et al. 2000

Morphology

Cell morphology

@ref	Gram stain	Confidence	Motility
125439	positive	99.689
125439		98.278

Colony morphology

@ref	Colony color	Incubation period	Medium used
19691	Brown beige	10-14 days	ISP 2
19691	Brown beige	10-14 days	ISP 3
19691	Brown beige	10-14 days	ISP 4
19691	Brown beige	10-14 days	ISP 5
19691	Brown beige	10-14 days	ISP 6
19691	Brown beige	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19691		ISP 2
19691		ISP 3
19691		ISP 4
19691		ISP 5
19691		ISP 6
19691		ISP 7

Multimedia

@ref:	11896
multimedia content:	DSM_44705.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44705.jpg
caption:	Medium 84 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11896	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
11896	ISP 5 MEDIUM (DSMZ Medium 993)	Medium recipe at MediaDive	Name: ISP 5 MEDIUM (DSMZ Medium 993) Composition: Agar 20.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l L-Asparagine 1.0 g/l FeSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l ZnSO4 x 7 H2O 0.001 g/l Distilled water
19691	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19691	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19691	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19691	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19691	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19691	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
11896	positive	growth	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	facultative anaerobe	97.924

Spore formation

@ref	Spore formation	Confidence
125439		96.784

Observation

67770

Observationquinones: MK-9(H₄), MK-9(H₂), MK-9(H₆)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity
19691	22599 ChEBI	arabinose	+
19691	62968 ChEBI	cellulose	+
19691	28757 ChEBI	fructose	+
19691	17234 ChEBI	glucose	-
19691	29864 ChEBI	mannitol	+
19691	17268 ChEBI	myo-inositol	+
19691	16634 ChEBI	raffinose	-
19691	26546 ChEBI	rhamnose	+
19691	17992 ChEBI	sucrose	+
19691	18222 ChEBI	xylose	+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19691	not determinedn.d.	+	+	+	-	+	+	-	-	-	+	-	-	-	-	+	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
11896	soil		Japan	JPN	Asia
67770	Soil	Saitama Pref.	Japan	JPN	Asia

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
11896	1	Risk group (German classification) According to TRBA 466
19691	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM3952538v1 assembly for Acrocarpospora macrocephala JCM 10982	scaffold	GCA_039525385			150177	25.63
66792	ASM968786v1 assembly for Acrocarpospora macrocephala NBRC 16266	scaffold	GCA_009687865	150177.3	2994239733	150177	23.02

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
11896	Acrocarpospora macrocephala gene for 16S ribosomal RNA	AB025318	1449		150177

GC content

@ref	GC-content (mol%)	Method
11896	68
67770	68.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM3952538v1 assembly for Acrocarpospora macrocephala JCM 10982
NCBI: GCA_039525385

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	97.92	no
125439	motility	BacteriaNetⓘ	no	98.28	no
125439	gram_stain	BacteriaNetⓘ	positive	99.69	no
125439	spore_formation	BacteriaNetⓘ	yes	96.78	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Acrocarpospora macrocephala NBRC 16266
NCBI: GCA_009687865.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	89.48	no
125438	anaerobic	anaerobicⓘ	no	93.22	no
125438	aerobic	aerobicⓘ	yes	86.83	no
125438	spore-forming	spore-formingⓘ	yes	86.63	no
125438	thermophilic	thermophileⓘ	no	92.99	yes
125438	flagellated	motile2+ⓘ	no	87.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters in the genus Acrocarpospora.	Komaki H, Oguchi A, Tamura T, Hamada M, Ichikawa N	J Gen Appl Microbiol	10.2323/jgam.2020.01.001	2020
Phylogeny	Acrocarpospora gen. nov., a new genus of the order Actinomycetales.	Tamura T, Suzuki S, Hatano K	Int J Syst Evol Microbiol	10.1099/00207713-50-3-1163	2000
Phylogeny	Acrocarpospora phusangensis sp. nov., isolated from a temperate peat swamp forest soil.	Niemhom N, Suriyachadkun C, Tamura T, Thawai C	Int J Syst Evol Microbiol	10.1099/ijs.0.046227-0	2012
Phylogeny	Acrocarpospora catenulata sp. nov., a novel actinobacterium isolated from lake sediment.	Liu C, Guo Y, Li L, Li J, Wang L, Lin J, Wang X, Chu Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.005526	2022

References

#11896	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44705
#19691	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Acrocarpospora macrocephala (DSM 44705, IFO 16266, JCM 10982)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039525385

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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