Streptomyces thinghirensis (DSM 41919, CCMM B35, JCM 18306)

Name: Streptomyces thinghirensis (DSM 41919, CCMM B35, JCM 18306)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 16465

Type strain

Strain Designation S10

Culture col. no. DSM 41919 CCMM B35 JCM 18306 S10

NCBI tax ID(s) 551547

Special Collections DSMZ JCM Wink compendium Literature strains

Links

Streptomyces thinghirensis S10 is a spore-forming, mesophilic prokaryote that builds an aerial mycelium and was isolated from rhizosphere soil of wild Vitis vinifera.

spore-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 24056

JCM 18306,LMG 21089,DSM 41919,CCMM B35

@ref 20215

Last LPSN update 2025-12-16 09:50:51

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Kitasatosporales

Family Streptomycetaceae

Genus Streptomyces

Species Streptomyces thinghirensis

Full scientific name Streptomyces thinghirensis Loqman et al. 2009

Morphology

Colony morphology

@ref	Colony color	Incubation period	Medium used
19829	Yellow orange	10-14 days	ISP 2
19829	Ochre yellow	10-14 days	ISP 3
19829	Green brown	10-14 days	ISP 4
19829	Ochre yellow	10-14 days	ISP 5
19829	Chrome yellow	10-14 days	ISP 6
19829	Brown beige	10-14 days	ISP 7

Multicellular morphology

@ref	Complex name	Complex color	Medium name
19829	Aerial Mycelium	Silver grey	ISP 2
19829	Aerial Mycelium	Silver grey	ISP 3
19829	Aerial Mycelium	Silver grey	ISP 4
19829	Aerial Mycelium	Light ivory	ISP 5
19829			ISP 6
19829	Aerial Mycelium	Light ivory	ISP 7

Pigmentation

29226

Productionyes

Multimedia

@ref:	10659
multimedia content:	DSM_41919.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_41919.jpg
caption:	Medium 65 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
10659	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19829	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19829	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19829	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19829	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19829	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19829	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
10659	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
10659	positive	growth	28	mesophilic
19829	positive	optimum	28	mesophilic
29226	positive	growth	28-42
29226	positive	optimum	35	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
29226	positive	optimum	7.5
29226	positive	growth	05-10	alkaliphile

Physiology and metabolism

Spore formation

29226

Spore formationyes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29226	NaCl	positive	growth	0-7 %

Observation

29226

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29226	17057 ChEBI	cellobiose	+	carbon source
29226	4853 ChEBI	esculin	+	hydrolysis
29226	28757 ChEBI	fructose	+	carbon source
29226	28260 ChEBI	galactose	+	carbon source
29226	17234 ChEBI	glucose	+	carbon source
29226	17716 ChEBI	lactose	+	carbon source
29226	17306 ChEBI	maltose	+	carbon source
29226	29864 ChEBI	mannitol	+	carbon source
29226	37684 ChEBI	mannose	+	carbon source
29226	17268 ChEBI	myo-inositol	+	carbon source
29226	17632 ChEBI	nitrate	+	reduction
29226	26546 ChEBI	rhamnose	+	carbon source
29226	30911 ChEBI	sorbitol	+	carbon source
29226	17992 ChEBI	sucrose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	+	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	+		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19829	not determinedn.d.	+	+	+	+	+	-	-	-	-	+	+	-	+	-	+	+	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Rhizosphere

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
10659	rhizosphere soil of wild Vitis vinifera	Vitis vinifera		Morocco	MAR	Africa
67770	Rhizosphere soil of wild healthy Vitis vinifera plants	Vitis vinifera	Thinghir, Ouarzazate Province, southern Morocco	Morocco	MAR	Africa

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
10659	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
124043	ASM3954340v1 assembly for Streptomyces thinghirensis JCM 18306	scaffold	GCA_039543405	551547	63.03

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
10659	Streptomyces thinghirensis partial 16S rRNA gene, strain S10	FM202482	1462		551547

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Streptomyces thinghirensis sp. nov., isolated from rhizosphere soil of Vitis vinifera.	Loqman S, Bouizgarne B, Barka EA, Clement C, von Jan M, Sproer C, Klenk HP, Ouhdouch Y	Int J Syst Evol Microbiol	10.1099/ijs.0.008946-0	2009

References

#10659	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 41919
#19829	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#25647	IJSEM 3063 2009 ( DOI 10.1099/ijs.0.008946-0 , PubMed 19643894 )
#29226	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25647
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Streptomyces thinghirensis (DSM 41919, CCMM B35, JCM 18306)

Name and taxonomic classification

Morphology

Colony morphology

Multicellular morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Spore formation

Halophily

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_039543405

16S sequences

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media