[Ref.: #10657] |
Culture medium |
GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | |
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[Ref.: #10657] |
Culture medium growth |
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[Ref.: #10657] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #10657] |
Culture medium composition |
expand / minimize |
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Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
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[Ref.: #10657] |
Culture medium |
BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | |
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[Ref.: #10657] |
Culture medium growth |
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[Ref.: #10657] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #10657] |
Culture medium composition |
expand / minimize |
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Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
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[Ref.: #19826] |
Culture medium |
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[Ref.: #19826] |
Culture medium growth |
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[Ref.: #19826] |
Culture medium composition |
expand / minimize |
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Name: ISP 2 / Yeast Malt Agar (5265); 5265
Composition
Malt extract 10.0 g/l
Yeast extract 4.0 g/l
Glucose 4.0 g/l
Agar 15.0 g/l
Preparation:
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.0
Usage: Maintenance and Taxonomy
Organisms: All Actinomycetes |
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[Ref.: #19826] |
Culture medium |
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[Ref.: #19826] |
Culture medium growth |
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[Ref.: #19826] |
Culture medium composition |
expand / minimize |
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Name: ISP 3; 5315
Composition
Dog oat flakes 20.0 g/l
Trace element solution (5314) 2.5 ml/l
Agar 18.0 g/l
Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated).
Sterilisation: 20 minutes at 121°C
pH before sterilisation: 7.8
Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production)
Organisms: All Actinomycetes
Trace element solution 5314
Name: Trace element solution 5314; 5314
Composition
CaCl2 x H2O 3.0 g/l
Fe-III-citrate 1.0 g/l
MnSO4 0.2 g/l
ZnCl2 0.1 g/l
CuSO4 x 5 H2O 0.025 g/l
Sodium tetra borate 0.2 g/l
CoCl2 x 6 H2O 0.004 g/l
Sodium molybdate 0.01 g/l
Preparation: Use double destillated water.
Sterilisation: 20 minutes at 121°C
pH before sterilisation:
Usage: Trace element solution for different media
Organisms: |
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[Ref.: #19826] |
Culture medium |
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[Ref.: #19826] |
Culture medium growth |
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[Ref.: #19826] |
Culture medium composition |
expand / minimize |
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Name: ISP 4; DSM 547
Solution I:
Difco soluble starch, 10.0 g.
Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml.
Solution II:
CaCO3 2.0 g
K2HPO4 (anhydrous) 1.0 g
MgSO4 x 7 H2O 1.0 g
NaCl 1.0 g
(NH4)2SO4 2.0 g
Distilled water 500.0 ml
Trace salt solution (see below) 1.0 ml
The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min.
Trace element solution:
FeSO4 x 7 H2O 0.1 g
MnCl2 x 4 H2O 0.1 g
ZnSO4 x 7 H2O 0.1 g
Distilled water 100.0 ml |
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[Ref.: #19826] |
Culture medium |
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[Ref.: #19826] |
Culture medium growth |
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[Ref.: #19826] |
Culture medium composition |
expand / minimize |
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Name: ISP 5 (5323)
Composition
L-Asparagine 1.0 g/l
Glycerol 10.0 g/l
K2HPO4 1.0 g/l
Salt solution (see preparation) 1.0 ml/l
Agar 20.0 g/l
Preparation:
Salt solution
1.0 g FeSO4 x 7 H2O
1.0 g MnCl2 x 4 H2O
1.0 g ZNSO4 x 7 H2O
in 100 ml water
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.2
Usage:
Maintenance and taxonomy
Organisms:
All Actinomycetes |
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[Ref.: #19826] |
Culture medium |
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[Ref.: #19826] |
Culture medium growth |
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[Ref.: #19826] |
Culture medium composition |
expand / minimize |
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Name: ISP 6 (5318)
Composition
Peptone 15.0 g/l
Proteose peptose 5.0 g/l
Ferric ammonium citrate 0.5 g/l
Sodium glycerophosphate 1.0 g/l
Sodium thiosulfate 0.08 g/l
Yeast extract 1.0 g/l
Agar 15.0 g/l
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
Usage: Production of melanoid pigments
Organisms:
All Actinomycetes |
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[Ref.: #19826] |
Culture medium |
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[Ref.: #19826] |
Culture medium growth |
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[Ref.: #19826] |
Culture medium composition |
expand / minimize |
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Name: ISP 7 (5322)
Composition
Glycerol 15.0 g/l
L-Tyrosine 0.5 g/l
L-Asparagine 1.0 g/l
K2HPO4 0.5 g/l
NaCl 0.5 g/l
FeSO4 x 7 H2O 0.01 g/l
Trace element solution 5343 1.0 ml/l
Agar 20.0
Sterilisation:
20 minutes at 121°C
pH before sterilisation:
7.3
Usage:
Production of melanoid pigments
Organisms:
All Actinomycetes |
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[Ref.: #37842] |
Culture medium |
MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulum | |
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[Ref.: #37842] |
Culture medium growth |
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[Ref.: #37842] |
Culture medium composition |
expand / minimize |
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Distilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g) |
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[Ref.: #10657] |
Culture medium |
TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | |
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[Ref.: #10657] |
Culture medium growth |
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[Ref.: #10657] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #10657] |
Culture medium composition |
expand / minimize |
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Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
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[Ref.: #10657] |
Culture medium |
GPHF-MEDIUM (DSMZ Medium 553) | |
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[Ref.: #10657] |
Culture medium growth |
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[Ref.: #10657] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #10657] |
Culture medium composition |
expand / minimize |
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Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water |
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[Ref.: #10657] |
Culture medium |
BHI MEDIUM (DSMZ Medium 215) | |
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[Ref.: #10657] |
Culture medium growth |
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[Ref.: #10657] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #10657] |
Culture medium composition |
expand / minimize |
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Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water |
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[Ref.: #10657] |
Culture medium |
ISP MEDIUM 4 (DSMZ Medium 547) | |
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[Ref.: #10657] |
Culture medium growth |
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[Ref.: #10657] |
Culture medium link |
Medium recipe at MediaDive |
[Ref.: #10657] |
Culture medium composition |
expand / minimize |
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Name: ISP MEDIUM 4 (DSMZ Medium 547; with strain-specific modifications) Composition: NaCl 30.0 g/l Agar 19.98 g/l Starch 9.99001 g/l (NH4)2SO4 1.998 g/l CaCO3 1.998 g/l K2HPO4 0.999002 g/l MgSO4 x 7 H2O 0.999002 g/l FeSO4 x 7 H2O 0.000999001 g/l MnCl2 x 4 H2O 0.000999001 g/l ZnSO4 x 7 H2O 0.000999001 g/l Distilled water |
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[Ref.: #123399] |
Culture medium |
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[Ref.: #123399] |
Culture medium growth |
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[Ref.: #123399] |
Culture medium link |
Medium recipe provided by DSMZ |