Ahniella affigens (KACC 19270, JCM 31634, D13)

Name: Ahniella affigens (KACC 19270, JCM 31634, D13)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 159867

Type strain

Strain Designation D13

Culture col. no. KACC 19270 JCM 31634 D13

NCBI tax ID(s) 2021234

Special Collections JCM Literature strains

Links

Ahniella affigens D13 is an aerobe, mesophilic, Gram-negative prokaryote that was isolated from sandy soil from a stream.

Gram-negative rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 133724

JCM 31634

@ref 20215

Last LPSN update 2025-12-16 10:08:21

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Lysobacterales

Family Rhodanobacteraceae

Genus Ahniella

Species Ahniella affigens

Full scientific name Ahniella affigens Hwang et al. 2018

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility	Confidence
66605	negative	rod-shaped
125438	negative			98

Colony morphology

@ref	Colony color	Incubation period	Medium used
66605	yellow	3 days	R2A medium

Culture and growth conditions

Culture medium

@ref	Name	Growth
66605	Nutrient agar (NA)
66605	Reasoner's 2A agar (R2A)
66605	tryptic soy agar (TSA)

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
66605	positive	optimum	30	mesophilic
66605	negative	growth	40
66605	positive	growth	15-35
67770	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH
66605	positive	optimum	7
66605	positive	growth	6.5-8.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
66605	aerobe
125439	obligate aerobe	99.5

Spore formation

@ref	Spore formation	Confidence
125438		91.262

Halophily

@ref	Salt	Growth	Tested relation	Concentration
66605	NaCl	positive	growth	0.5 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
66605	85146 ChEBI	carboxymethylcellulose	+	hydrolysis
66605		casein	+	hydrolysis
66605	27689 ChEBI	decanoate	+/-	assimilation
66605	4853 ChEBI	esculin	-	hydrolysis
66605	28757 ChEBI	fructose	-	builds acid from
66605	5291 ChEBI	gelatin	+	hydrolysis
66605	17234 ChEBI	glucose	-	builds acid from
66605	17306 ChEBI	maltose	-	builds acid from
66605	29864 ChEBI	mannitol	-	builds acid from
66605	17632 ChEBI	nitrate	-	reduction
66605	30911 ChEBI	sorbitol	-	builds acid from
66605	28017 ChEBI	starch	+	hydrolysis
66605	17992 ChEBI	sucrose	-	builds acid from
66605	53426 ChEBI	tween 80	+	hydrolysis
66605	18186 ChEBI	tyrosine	-	hydrolysis
66605	18222 ChEBI	xylose	-	builds acid from

Metabolite production

@ref	Chebi-ID	Metabolite	Production
66605	16136 ChEBI	hydrogen sulfide

Enzymes

@ref	Value	Activity	Ec
66605	acid phosphatase	-	3.1.3.2
66605	alanine arylamidase	+	3.4.11.2
66605	alkaline phosphatase	+	3.1.3.1
66605	alpha-chymotrypsin	+	3.4.21.1
66605	alpha-fucosidase	-	3.2.1.51
66605	alpha-galactosidase	-	3.2.1.22
66605	alpha-glucosidase	-	3.2.1.20
66605	alpha-mannosidase	-	3.2.1.24
66605	beta-galactosidase	-	3.2.1.23
66605	beta-glucosidase	-	3.2.1.21
66605	beta-glucuronidase	-	3.2.1.31
66605	catalase	-	1.11.1.6
66605	cystine arylamidase	-	3.4.11.3
66605	cytochrome oxidase	+	1.9.3.1
66605	esterase (C 4)	-
66605	esterase Lipase (C 8)	+/-
66605	leucine arylamidase	+	3.4.11.1
66605	lipase (C 14)	-
66605	N-acetyl-beta-glucosaminidase	+/-	3.2.1.52
66605	naphthol-AS-BI-phosphohydrolase	+
66605	phenylalanine arylamidase	+
66605	proline-arylamidase	+	3.4.11.5
66605	trypsin	+	3.4.21.4
66605	valine arylamidase	+/-

Fatty acid profile

Metadata FA analysis

@ref

66605

Fatty acid	Percentage
C16:0 iso	35
C15:0 iso	16.5
C17:1 iso w9c / C16:0 10-methyl	12.9
C15:1 iso H / C13:0 3OH	8.2
C16:1 iso H	5.6
C11:0 iso 3OH	3.9
C11:0 iso	3.5
C14:0 iso	3.2
C16:1 w6c / C16:1 w7c	2.6
C17:0 iso	2.6
C18:0 iso	0.7
C10:0 iso	0.6
C18:1 w7c / C18:1 w6c	0.5
C12:0 iso	0.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Sandy
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture duration	Enrichment culture temperature
66605	sandy soil from a stream	Sinan-gun	Republic of Korea	KOR	Asia	34.95	126.133 34.95/126.133	R2A agar	5 days	30
67770	Sandy soil from a stream in Sinan-gun		Republic of Korea	KOR	Asia

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM301518v1 assembly for Ahniella affigens D13	complete	GCA_003015185	2021234.3	2833611726	2021234	97.5

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
66605	Ahniella affigens strain D13 16S ribosomal RNA gene, partial sequence	KY649437	1476		2021234

GC content

@ref	GC-content (mol%)	Method
66605	57.70	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Xanthomonadales bacterium D13
PATRIC: 2021234.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	85.70	no
125439	motility	BacteriaNetⓘ	no	60.60	no
125439	gram_stain	BacteriaNetⓘ	negative	68.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.50	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Ahniella affigens D13
NCBI: GCA_003015185.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.00	yes
125438	anaerobic	anaerobicⓘ	no	95.21	yes
125438	aerobic	aerobicⓘ	yes	84.51	no
125438	spore-forming	spore-formingⓘ	no	91.26	no
125438	thermophilic	thermophileⓘ	no	97.40	yes
125438	flagellated	motile2+ⓘ	no	61.43	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ahniella affigens gen. nov., sp. nov., a gammaproteobacterium isolated from sandy soil near a stream.	Hwang WM, Ko Y, Kim JH, Kang K	Int J Syst Evol Microbiol	10.1099/ijsem.0.002859	2018

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66605	Woon Mo Hwang, Yongseok Ko, Jae-Heon Kim and Keunsoo Kang: Ahniella affigens gen. nov., sp. nov., a gammaproteobacterium isolated from sandy soil near a stream. IJSEM 68: 2478 - 2484 2018 ( DOI 10.1099/ijsem.0.002859 , PubMed 29923816 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Ahniella affigens (KACC 19270, JCM 31634, D13)

Name and taxonomic classification