Name: Ottowia oryzae KADR8-3
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 159829

Type strain:

Species: Ottowia oryzae

Strain Designation: KADR8-3

Culture col. no.: KACC 19325, NBRC 113109

NCBI tax ID(s): 2109914 (species)

Special Collections

Literature strains

Section

Ottowia oryzae KADR8-3 is a Gram-negative, rod-shaped bacterium that was isolated from Sikhye from Andongsi.

Gram-negative
rod-shaped
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Betaproteobacteria*
	Order	*Burkholderiales*
	Family	*Comamonadaceae*
	Genus	*Ottowia*
	Species	**Ottowia oryzae**
	Full Scientific Name (LPSN)	Ottowia oryzae Heo et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66664]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98

[Ref.: #66664]	Cell length	1.4-1.8 µm

[Ref.: #66664]	Cell width	0.9 µm

[Ref.: #66664]	Cell shape	rod-shaped

[Ref.: #66664]	Motility	no

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

R2A broth

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66664]			growth	10-37 °C
[Ref.: #66664]			optimum	28-37 °C

	pH	Kind of pH		pH
[Ref.: #66664]			optimum	7.0-8.0
[Ref.: #66664]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66664]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %91.187
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66664]		NaCl		growth	0-3	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66664]	3-hydroxybenzoate ChEBI	-	assimilation
[Ref.: #66664]	3-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #66664]	4-hydroxybenzoate ChEBI	+	assimilation
[Ref.: #66664]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #66664]	casein	+	hydrolysis
[Ref.: #66664]	D-glucose ChEBI	-	assimilation
[Ref.: #66664]	D-mannitol ChEBI	-	assimilation
[Ref.: #66664]	D-ribose ChEBI	-	assimilation
[Ref.: #66664]	D-sorbitol ChEBI	-	assimilation
[Ref.: #66664]	decanoate ChEBI	-	assimilation
[Ref.: #66664]	dna ChEBI	-	hydrolysis
[Ref.: #66664]	esculin ChEBI	+	hydrolysis
[Ref.: #66664]	gelatin ChEBI	+	hydrolysis
[Ref.: #66664]	glycogen ChEBI	-	assimilation
[Ref.: #66664]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #66664]	itaconate ChEBI	-	assimilation
[Ref.: #66664]	L-alanine ChEBI	+	assimilation
[Ref.: #66664]	L-arabinose ChEBI	-	assimilation
[Ref.: #66664]	L-fucose ChEBI	-	assimilation
[Ref.: #66664]	L-histidine ChEBI	-	assimilation
[Ref.: #66664]	L-proline ChEBI	+	assimilation
[Ref.: #66664]	L-rhamnose ChEBI	-	assimilation
[Ref.: #66664]	L-serine ChEBI	-	assimilation
[Ref.: #66664]	lactate ChEBI	+	assimilation
[Ref.: #66664]	maltose ChEBI	-	assimilation
[Ref.: #66664]	melibiose ChEBI	-	assimilation
[Ref.: #66664]	myo-inositol ChEBI	-	assimilation
[Ref.: #66664]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #66664]	nitrate ChEBI	+	reduction
[Ref.: #66664]	potassium 2-dehydro-D-gluconate	-	assimilation
[Ref.: #66664]	potassium 5-dehydro-D-gluconate	-	assimilation
[Ref.: #66664]	propionate ChEBI	+	assimilation
[Ref.: #66664]	salicin ChEBI	-	assimilation
[Ref.: #66664]	sodium acetate ChEBI	+	assimilation
[Ref.: #66664]	sodium citrate ChEBI	+	assimilation
[Ref.: #66664]	sodium malonate ChEBI	-	assimilation
[Ref.: #66664]	starch ChEBI	-	hydrolysis
[Ref.: #66664]	suberic acid ChEBI	+	assimilation
[Ref.: #66664]	sucrose ChEBI	-	assimilation
[Ref.: #66664]	tween 80 ChEBI	+	hydrolysis
[Ref.: #66664]	tyrosine ChEBI	+	hydrolysis
[Ref.: #66664]	valerate ChEBI	+	assimilation
[Ref.: #66664]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #66664]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #66664]	acid phosphatase	+	3.1.3.2
[Ref.: #66664]	alkaline phosphatase	+	3.1.3.1
[Ref.: #66664]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #66664]	alpha-fucosidase	-	3.2.1.51
[Ref.: #66664]	alpha-galactosidase	-	3.2.1.22
[Ref.: #66664]	alpha-glucosidase	-	3.2.1.20
[Ref.: #66664]	alpha-mannosidase	-	3.2.1.24
[Ref.: #66664]	arginine dihydrolase	-	3.5.3.6
[Ref.: #66664]	beta-galactosidase	-	3.2.1.23
[Ref.: #66664]	beta-glucosidase	-	3.2.1.21
[Ref.: #66664]	beta-glucuronidase	-	3.2.1.31
[Ref.: #66664]	catalase	+	1.11.1.6
[Ref.: #66664]	cystine arylamidase	-	3.4.11.3
[Ref.: #66664]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66664]	esterase (C 4)	+
[Ref.: #66664]	esterase Lipase (C 8)	+
[Ref.: #66664]	leucine arylamidase	+	3.4.11.1
[Ref.: #66664]	lipase	-
[Ref.: #66664]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #66664]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #66664]	trypsin	-	3.4.21.4
[Ref.: #66664]	urease	+	3.5.1.5
[Ref.: #66664]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #66664]

Fatty acid	Percentage
C16:1 w6c / C16:1 w7c	30.6
C16:0	29.7
C18:1 w6c / C18:1 w7c	19.2
C17:0 cyclo	7.8
C14:0	3.5
C12:0	3
C10:0 3OH	2.3
C12:0 3OH	1.6
C14:0 2OH	0.9

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66664]	Sample type/isolated from	Sikhye from Andongsi
[Ref.: #66664]	Geographic location (country and/or sea, region)	Andongsi, Gyeongsangbuk-do
[Ref.: #66664]	Country	Republic of Korea
[Ref.: #66664]	Country ISO 3 Code	KOR
[Ref.: #66664]	Continent	Asia
[Ref.: #66664]	Enrichment culture	Reasoner's 2A agar
[Ref.: #66664]	Enrichment culture composition	supplemented with cycloheximide (200 µg ml-1)
[Ref.: #66664]	Enrichment culture duration	3 days
[Ref.: #66664]	Enrichment culture temperature	28 ̊C
[Ref.: #66664]	Isolation/enrichment procedure	The sikhye sample was transferred into sterile homogenization bag, and the homogenate was serial diluted, and spread on Reasoner's 2A agar and incubated for 3days at 28°C.
* marker position based on {}

Isolation sources categories

#Engineered

#Food production

#Beverage

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence MH031704 (>99% sequence identity) for Ottowia oryzae subclade from Microbeatlas

Aquatic Samples	691
Soil Samples	2514
Animal Samples	260
Plant Samples	495
Total Samples	3960

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66664]	Ottowia oryzae strain KADR8-3 16S ribosomal RNA gene, partial sequence	MH031704	1525	GenBank ENA	2109914 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Ottowia oryzae KADR8-3	GCA_003008535	complete	GenBank ENA	2109914 tax ID	*
[Ref.: #66792]	Ottowia sp. KADR8-3	2109914.3	complete	PATRIC	2109914 tax ID	*

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GC-content

66.8 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Ottowia oryzae KADR8-3 NCBI: GCA_003008535.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Ottowia oryzae KADR8-3 NCBI: GCA_003008535.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98	yes
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	97.753	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	84.839	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	91.187	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	95.219	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	58.852	no

Availability in culture collections External links

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Culture collection no.

KACC 19325, NBRC 113109

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Ottowia oryzae sp. nov., isolated from Andong sikhye, a Korean traditional rice beverage.	Heo J, Cho H, Hong SB, Kim JS, Kwon SW, Kim SJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002935	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66664

Jun Heo, Hayoung Cho, Seung-Beom Hong, Jeong-Seon Kim, Soon-Wo Kwon, Soo-Jin Kim: Ottowia oryzae sp. nov., isolated from Andong sikhye, a Korean traditional rice beverage. IJSEM 68: 3096 - 3100 2018 ( DOI 10.1099/ijsem.0.002935 , PubMed 30102146 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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