Name: Microbispora soli RTBAU4-9
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 159813

Type strain:

Species: Microbispora soli

Strain Designation: RTBAU4-9

Culture col. no.: TBRC 7648, NBRC 113147

NCBI tax ID(s): 2053007 (species)

Section

Microbispora soli RTBAU4-9 is a spore-forming, thermophilic, Gram-positive bacterium that builds aerial mycelia and was isolated from hot spring soil sample.

Gram-positive
spore-forming
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Microbispora*
	Species	**Microbispora soli**
	Full Scientific Name (LPSN)	Microbispora soli Kittisrisopit et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #66778]	Gram stain	positive

[Ref.: #66778]	Colony color	pale yellowish brown
[Ref.: #66778]	Cultivation medium used	ISP 2

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Pigment production

[Ref.: #66778]	Multicellular complex forming ability	yes
[Ref.: #66778]	Multicellular complex name	aerial mycelia
[Ref.: #66778]	Multicellular complex further description	White to yellowish white aerial mycelium was produced easily on ISP5 and ISP7 agar medium, but not on ISP2, ISP3, ISP4, ISP6, Czapek's sucrose or nutrient media.

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Czapek sucrose

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Culture medium growth

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Culture medium

glucose asparagine

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Culture medium growth

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

ISP 3

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Culture medium growth

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Culture medium

ISP 4

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Culture medium growth

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Culture medium

ISP 5

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Culture medium growth

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Culture medium

ISP 6

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Culture medium growth

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Culture medium

nutrient media

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #66778]			optimum	40 °C
[Ref.: #66778]			growth	25-55 °C

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Temperature range

thermophilic

	pH	Kind of pH		pH
[Ref.: #66778]			optimum	6-7
[Ref.: #66778]			growth	5-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #66778]	Spore description	possess longitudinally paired spores (0.8–1.0 0.9–1.2 µm) borne directly on aerial mycelia. The surface of spores was smooth.
[Ref.: #66778]	Type of spore	spore
[Ref.: #66778]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #66778]		NaCl		optimum	4	%(w/v)

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Observation

The predominant menaquinones are MK-9(H4), MK-9(H2), MK-9(H6) and MK-9(H0).

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #66778]	2-aminobutyrate ChEBI	+	nitrogen source
[Ref.: #66778]	adenine ChEBI	-	degradation
[Ref.: #66778]	cellobiose ChEBI	+	builds acid from
[Ref.: #66778]	crystalline cellulose	-	hydrolysis
[Ref.: #66778]	D-fructose ChEBI	+	builds acid from
[Ref.: #66778]	D-galactose ChEBI	+	builds acid from
[Ref.: #66778]	D-glucose ChEBI	+	builds acid from
[Ref.: #66778]	D-mannitol ChEBI	+	builds acid from
[Ref.: #66778]	D-ribose ChEBI	+	builds acid from
[Ref.: #66778]	D-xylose ChEBI	+	builds acid from
[Ref.: #66778]	dextran ChEBI	-	builds acid from
[Ref.: #66778]	glycerol ChEBI	+	builds acid from
[Ref.: #66778]	hydroxyproline ChEBI	-	nitrogen source
[Ref.: #66778]	hypoxanthine ChEBI	+	hydrolysis
[Ref.: #66778]	L-arginine ChEBI	+	nitrogen source
[Ref.: #66778]	L-asparagine ChEBI	+	nitrogen source
[Ref.: #66778]	L-cysteine ChEBI	+	nitrogen source
[Ref.: #66778]	L-histidine ChEBI	+	nitrogen source
[Ref.: #66778]	L-methionine ChEBI	-	nitrogen source
[Ref.: #66778]	L-phenylalanine ChEBI	+	nitrogen source
[Ref.: #66778]	L-proline ChEBI	+	nitrogen source
[Ref.: #66778]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #66778]	L-serine ChEBI	+	nitrogen source
[Ref.: #66778]	L-threonine ChEBI	+	nitrogen source
[Ref.: #66778]	L-valine ChEBI	+	nitrogen source
[Ref.: #66778]	lactose ChEBI	-	builds acid from
[Ref.: #66778]	melezitose ChEBI	-	builds acid from
[Ref.: #66778]	melibiose ChEBI	-	builds acid from
[Ref.: #66778]	raffinose ChEBI	-	builds acid from
[Ref.: #66778]	ribitol ChEBI	-	builds acid from
[Ref.: #66778]	salicin ChEBI	-	builds acid from
[Ref.: #66778]	starch ChEBI	+	hydrolysis
[Ref.: #66778]	sucrose ChEBI	+	builds acid from
[Ref.: #66778]	trehalose ChEBI	+	builds acid from
[Ref.: #66778]	tyrosine ChEBI	-	degradation
[Ref.: #66778]	xanthine ChEBI	-	hydrolysis
[Ref.: #66778]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #66778]	catalase	+	1.11.1.6
[Ref.: #66778]	cytochrome oxidase	+	1.9.3.1
[Ref.: #66778]	nitrate reductase	+	1.7.99.4
[Ref.: #66778]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #66778]	Sample type/isolated from	hot spring soil sample
[Ref.: #66778]	Geographic location (country and/or sea, region)	Ratchaburi province
[Ref.: #66778]	Country	Thailand
[Ref.: #66778]	Country ISO 3 Code	THA
[Ref.: #66778]	Continent	Asia
[Ref.: #66778]	Enrichment culture	soil extract agar
[Ref.: #66778]	Enrichment culture composition	1 g soluble starch, 0.1 g KNO3, 0.005 g FeSO4.7H2O, 0.005 g MgSO4.7H2O, 0.001 g CaCl2.2H2O, 1.5 g agar, 100 ml soil extract solution; pH 7.3) supplemented with 20 mg nalidixic acid and 50 mg nystatin /L.
[Ref.: #66778]	Enrichment culture duration	14 days
[Ref.: #66778]	Enrichment culture temperature	40 ̊C
[Ref.: #66778]	Isolation/enrichment procedure	The soil sample was air-dried at room temperature for 3 days. The dried soil sample was then ground and heated at 120 C for 1 h. The treated soil was kept continuously in a desiccator containing silica gel beads for 7 days. The soil sample was then diluted 1000-fold with 0.01% sterile SDS in distilled water and then 0.1 ml of the diluted sample was spread onto soil extract agar
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Environmental	#Terrestrial	#Soil
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66778]	Microbispora soli RTBAU4-9 gene for 16S ribosomal RNA, partial sequence	LC333392	1493	ENA	2053007 tax ID

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GC-content

70.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

TBRC 7648, NBRC 113147

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Microbispora soli sp. nov., isolated from soil of a hot spring.	Kittisrisopit S, Pittayakhajonwut P, Tadtong S, Thawai C	Int J Syst Evol Microbiol	10.1099/ijsem.0.003075	2018	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#66778

Suchada Kittisrisopit, Pattama Pittayakhajonwut, Sarin Tadtong and Chitti Thawai: Microbispora soli sp. nov., isolated from soil of a hot spring. IJSEM 68: 3863 - 3868 2018 ( DOI 10.1099/ijsem.0.003075 , PubMed 30325294 )

* These data were automatically processed and therefore are not curated

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